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PMC:7094172 / 4753-24046 JSONTXT

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LitCovid-PMC-OGER-BB

Id Subject Object Predicate Lexical cue
T760 17830-17838 SP_9 denotes MERS-CoV
T759 17845-17854 GO:0032991 denotes complexed
T758 17867-17876 CHEBI:36357;CHEBI:36357 denotes compounds
T757 18015-18023 SP_9 denotes MERS-CoV
T756 18033-18040 GO:0032991 denotes complex
T755 18105-18113 SP_9 denotes MERS-CoV
T754 18123-18130 GO:0032991 denotes complex
T753 18192-18201 CHEBI:36357;CHEBI:36357 denotes compounds
T752 18263-18269 SO:0000440 denotes vector
T751 18317-18327 CHEBI:39015;CHEBI:39015 denotes apoprotein
T750 18443-18451 SP_9 denotes MERS-CoV
T749 18584-18596 SO:0000409 denotes binding site
T748 18765-18773 SP_9 denotes MERS-CoV
T747 18965-18977 SO:0000409 denotes binding site
T746 19145-19153 SP_9 denotes MERS-CoV
T731 14666-14675 CHEBI:35222;CHEBI:35222 denotes inhibitor
T730 14684-14692 SP_9 denotes MERS-CoV
T729 14733-14741 SP_9 denotes MERS-CoV
T728 14882-14886 SP_9 denotes MERS
T727 15094-15102 SP_9 denotes MERS-CoV
T726 15103-15123 PG_4 denotes nucleocapsid protein
T725 15159-15179 PG_4 denotes Nucleocapsid protein
T724 15180-15191 GO:0010467 denotes expressions
T723 15249-15255 GO:0005634 denotes Nuclei
T722 15279-15283 CHEBI:51231;CHEBI:51231 denotes DAPI
T715 9112-9120 SP_9 denotes MERS-CoV
T714 9121-9130 PG_4 denotes N protein
T713 9171-9179 SP_9 denotes MERS-CoV
T712 9180-9189 PG_4 denotes N protein
T711 9345-9354 PG_4 denotes N protein
T710 9368-9375 GO:0032991 denotes complex
T709 9460-9469 PG_4 denotes N protein
T708 9487-9494 GO:0032991 denotes complex
T707 9587-9596 PG_4 denotes N protein
T706 9614-9621 GO:0032991 denotes complex
T705 9835-9843 SP_9 denotes MERS-CoV
T704 9844-9853 PG_4 denotes N protein
T703 9927-9931 CHEBI:9754;CHEBI:9754 denotes Tris
T702 9932-9935 CHEBI:17883;CHEBI:17883 denotes HCl
T701 9944-9948 CHEBI:26710;CHEBI:26710 denotes NaCl
T700 10109-10121 GO:0046983 denotes dimerization
T699 10153-10165 GO:0046983 denotes dimerization
T698 10268-10277 PG_4 denotes N protein
T697 10381-10388 SO:0000409 denotes surface
T696 10441-10458 GO:0005840 denotes ribonucleocapsids
T680 7742-7750 SP_9 denotes MERS-CoV
T679 7751-7760 PG_4 denotes N protein
T678 7952-7961 PG_4 denotes N protein
T677 8187-8191 CHEBI:9754;CHEBI:9754 denotes Tris
T676 8192-8195 CHEBI:17883;CHEBI:17883 denotes HCl
T675 8216-8220 CHEBI:26710;CHEBI:26710 denotes NaCl
T669 3854-3862 SP_9 denotes MERS-CoV
T668 3899-3907 SP_9 denotes MERS-CoV
T667 4038-4050 GO:0046983 denotes dimerization
T666 4118-4126 SP_9 denotes MERS-CoV
T665 4135-4147 GO:0051260 denotes Dimerization
T664 4170-4176 SO:0000440 denotes vector
T663 4703-4709 SO:0000440 denotes vector
T662 4984-4990 SO:0000440 denotes vector
T661 5258-5276 SO:0000366 denotes substitution sites
T660 5318-5330 GO:0046983 denotes dimerization
T369 15307-15315 SP_9 denotes MERS-CoV
T368 15322-15331 GO:0032991 denotes Complexed
T367 15389-15397 SP_9 denotes MERS-CoV
T366 15407-15414 GO:1990524 denotes complex
T365 15420-15429 CHEBI:36357;CHEBI:36357 denotes compounds
T364 15515-15522 GO:0032991 denotes complex
T363 15660-15669 GO:0032991 denotes complexes
T362 15692-15700 SP_9 denotes MERS-CoV
T361 15707-15716 GO:0032991 denotes complexes
T360 15845-15854 CHEBI:36357;CHEBI:36357 denotes compounds
T359 15859-15867 SP_9 denotes MERS-CoV
T358 15915-15924 PG_4 denotes N protein
T357 15934-15942 CHEBI:36357;CHEBI:36357 denotes compound
T356 16116-16123 GO:0032991 denotes complex
T355 16348-16353 CHEBI:22563;CHEBI:22563 denotes anion
T354 16378-16390 CHEBI:52879;CHEBI:52879 denotes benzene ring
T353 16401-16409 CHEBI:43295;CHEBI:43295 denotes indoline
T352 16500-16507 CHEBI:52879;CHEBI:52879 denotes benzene
T351 16526-16536 CHEBI:50325;CHEBI:50325 denotes side chain
T350 16732-16746 SO:0000409 denotes binding region
T349 17041-17050 PG_4 denotes N protein
T348 17125-17137 SO:0000409 denotes binding site
T347 17185-17192 CHEBI:52879;CHEBI:52879 denotes benzene
T346 17375-17381 CHEBI:36916;CHEBI:36916 denotes cation
T345 17439-17445 CHEBI:24433;CHEBI:24433 denotes groups
T344 17795-17804 CHEBI:36357;CHEBI:36357 denotes compounds
T321 13114-13122 SP_9 denotes MERS-CoV
T320 13135-13144 PG_4 denotes N Protein
T319 13260-13268 SP_9 denotes MERS-CoV
T318 13329-13342 GO:0005576 denotes extracellular
T317 13343-13348 NCBITaxon:10239 denotes viral
T316 13360-13373 GO:0005622 denotes intracellular
T315 13374-13379 NCBITaxon:10239 denotes viral
T314 13425-13429 CL_6 denotes Vero
T313 13530-13535 NCBITaxon:10239 denotes viral
T312 13568-13573 NCBITaxon:10239;GO:0006260 denotes viral
T311 13574-13589 GO:0039703 denotes RNA replication
T310 13624-13629 NCBITaxon:10239;GO:0006260 denotes viral
T309 13630-13640 GO:0006260 denotes production
T308 13645-13656 GO:0006260 denotes replication
T307 13723-13731 CHEBI:36357;CHEBI:36357 denotes compound
T306 13750-13758 SP_9 denotes MERS-CoV
T305 13759-13768 PG_4 denotes N protein
T304 13786-13796 GO:0010467 denotes expression
T303 13947-13960 GO:0005622 denotes intracellular
T302 13961-13970 PG_4 denotes N protein
T301 14003-14007 CL_6 denotes Vero
T300 14060-14073 GO:0005622 denotes intracellular
T299 14074-14083 PG_4 denotes N protein
T298 14122-14123 PG_4 denotes N
T297 14124-14131 PG_4;BV_20 denotes protein
T296 14132-14142 BV_20;GO:0010467 denotes expression
T295 14196-14205 PG_4 denotes N protein
T294 14242-14250 SP_9 denotes MERS-CoV
T293 14418-14426 SP_9 denotes MERS-CoV
T292 14477-14485 SP_9 denotes MERS-CoV
T291 14526-14535 PG_4 denotes N protein
T272 8606-8614 SP_9 denotes MERS-CoV
T271 8615-8624 PG_4 denotes N Protein
T270 8697-8705 SP_9 denotes MERS-CoV
T269 8706-8715 PG_4 denotes N protein
T268 8988-8996 SP_9 denotes MERS-CoV
T267 8997-9006 PG_4 denotes N protein
T266 9010-9018 CHEBI:75958;CHEBI:75958 denotes solution
T265 10525-10534 PG_4 denotes N protein
T264 10680-10686 SO:0000417 denotes domain
T263 10734-10741 SO:0000417 denotes domains
T262 10821-10830 PG_4 denotes N protein
T261 10839-10846 GO:0032991 denotes complex
T260 11030-11039 PG_4 denotes N protein
T259 11101-11109 CHEBI:75958;CHEBI:75958 denotes solution
T258 11147-11155 CHEBI:75958;CHEBI:75958 denotes solution
T257 11244-11251 GO:0032991 denotes complex
T256 11542-11550 SP_9 denotes MERS-CoV
T255 11551-11560 PG_4 denotes N protein
T254 11628-11637 PG_4 denotes N protein
T253 11832-11844 GO:0046983 denotes dimerization
T252 11855-11863 SP_9 denotes MERS-CoV
T251 11879-11888 PG_4 denotes N protein
T250 11902-11907 NCBITaxon:10239 denotes viral
T249 11908-11914 SO:0001026 denotes genome
T248 11923-11926 GO:1990904 denotes RNP
T247 11927-11934 GO:0005662 denotes complex
T246 12305-12308 SO:0000694 denotes RNP
T245 12941-12950 PG_4 denotes N protein
T244 12951-12960 CHEBI:36357;CHEBI:36357 denotes molecules
T243 13043-13051 SP_9 denotes MERS-CoV
T242 13078-13087 PG_4 denotes N protein
T219 5620-5629 CHEBI:36357;CHEBI:36357 denotes compounds
T218 5653-5659 SO:0000440 denotes vector
T217 6238-6242 CHEBI:23888;CHEBI:23888 denotes drug
T216 6356-6365 CHEBI:36357;CHEBI:36357 denotes compounds
T215 6405-6411 CHEBI:22744;CHEBI:22744 denotes Benzyl
T214 6411-6415 CHEBI:75508;CHEBI:75508 denotes -2-(
T213 6415-6428 CHEBI:24712;CHEBI:24712 denotes hydroxymethyl
T212 6428-6451 CHEBI:75508;CHEBI:75508 denotes )-1-indolinecarboxylate
T211 6461-6462 CHEBI:75508;CHEBI:75508 denotes 5
T210 6463-6479 CHEBI:41237;CHEBI:41237 denotes benzyloxygramine
T209 6549-6553 CHEBI:23888;CHEBI:23888 denotes drug
T208 6554-6562 CHEBI:4909;CHEBI:4909 denotes etodolac
T207 6964-6973 PG_4 denotes N protein
T206 7111-7118 GO:0032508 denotes melting
T205 7195-7202 GO:0032508 denotes melting
T204 7213-7221 SP_9 denotes MERS-CoV
T203 7272-7292 GO:0030164 denotes protein denaturation
T202 7325-7333 SP_9 denotes MERS-CoV
T201 7465-7473 CHEBI:36357;CHEBI:36357 denotes compound
T200 7480-7484 CL_6 denotes Vero
T199 7508-7516 SP_9 denotes MERS-CoV
T198 7589-7598 CHEBI:36357;CHEBI:36357 denotes compounds
T197 7661-7670 CHEBI:35222;CHEBI:35222 denotes inhibitor
T196 7679-7687 SP_9 denotes MERS-CoV
T170 37-43 SO:0000417 denotes Domain
T169 51-59 SP_9 denotes MERS-CoV
T168 60-69 PG_4 denotes N Protein
T167 109-117 SP_9 denotes MERS-CoV
T166 404-413 CHEBI:36357;CHEBI:36357 denotes molecules
T165 630-643 SO:0000984 denotes five-stranded
T164 1058-1066 SP_9 denotes MERS-CoV
T163 1067-1076 PG_4 denotes N protein
T162 1187-1199 SO:0000409 denotes binding site
T161 1360-1369 PG_4 denotes N protein
T160 1489-1499 CHEBI:50325;CHEBI:50325 denotes side chain
T159 1717-1728 CHEBI:50325;CHEBI:50325 denotes side chains
T158 1979-1990 CHEBI:50325;CHEBI:50325 denotes side chains
T157 2075-2085 CHEBI:50325;CHEBI:50325 denotes side chain
T156 2546-2552 SO:0000440 denotes vector
T155 2755-2764 PG_4 denotes N protein
T154 2846-2850 SP_9 denotes MERS
T153 2872-2878 SO:0000440 denotes vector
T152 2898-2906 CHEBI:75958;CHEBI:75958 denotes solution
T151 2908-2912 SP_9 denotes MERS
T150 2949-2957 CHEBI:75958;CHEBI:75958 denotes solution
T149 3069-3075 SO:0000440 denotes vector
T148 3298-3304 SO:0000440 denotes vector
T147 3421-3429 SP_9 denotes MERS-CoV
T146 3530-3540 CHEBI:50325;CHEBI:50325 denotes side chain
T145 3714-3723 CHEBI:36357;CHEBI:36357 denotes compounds
T144 3739-3745 SO:0000440 denotes vector

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T32 62-69 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T33 704-716 Body_part denotes right-handed http://purl.org/sig/ont/fma/fma9713
T34 877-885 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T35 1069-1076 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T36 1157-1167 Body_part denotes amino acid http://purl.org/sig/ont/fma/fma82739
T37 1362-1369 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T38 1787-1795 Body_part denotes backbone http://purl.org/sig/ont/fma/fma13478
T39 2300-2302 Body_part denotes Å2 http://purl.org/sig/ont/fma/fma66595
T40 2757-2764 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T41 3305-3313 Body_part denotes backbone http://purl.org/sig/ont/fma/fma13478
T42 4796-4803 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T43 5145-5153 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T44 6255-6260 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T45 6811-6822 Body_part denotes tryptophans http://purl.org/sig/ont/fma/fma82767
T46 6830-6837 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T47 6966-6973 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T48 7272-7279 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T49 7488-7493 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T50 7753-7760 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T51 7954-7961 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T52 8009-8017 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T53 8553-8558 Body_part denotes d NA http://purl.org/sig/ont/fma/fma74412
T54 8617-8624 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T55 8708-8715 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T56 8776-8783 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T57 8874-8880 Body_part denotes radius http://purl.org/sig/ont/fma/fma23463
T58 8905-8912 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T59 8999-9006 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T60 9123-9130 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T61 9182-9189 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T62 9294-9300 Body_part denotes radius http://purl.org/sig/ont/fma/fma23463
T63 9347-9354 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T64 9462-9469 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T65 9589-9596 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T66 9846-9853 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T67 10035-10038 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T68 10042-10050 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T69 10242-10249 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T70 10270-10277 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T71 10369-10372 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T72 10527-10534 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T73 10629-10633 Body_part denotes body http://purl.org/sig/ont/fma/fma256135
T74 10823-10830 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T75 10953-10960 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T76 11032-11039 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T77 11223-11231 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T78 11553-11560 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T79 11630-11637 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T80 11738-11745 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T81 11881-11888 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T82 11908-11914 Body_part denotes genome http://purl.org/sig/ont/fma/fma84116
T83 12126-12132 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T84 12163-12166 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T85 12263-12269 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T86 12354-12357 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T87 12480-12486 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T88 12509-12512 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T89 12696-12703 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T90 12819-12826 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T91 12880-12883 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T92 12943-12950 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T93 13080-13087 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T94 13137-13144 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T95 13291-13295 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T96 13380-13383 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T97 13433-13438 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T98 13574-13577 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T99 13761-13768 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T100 13813-13818 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T101 13963-13970 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T102 14011-14016 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T103 14076-14083 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T104 14124-14131 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T105 14151-14156 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T106 14198-14205 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T107 14253-14261 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T108 14282-14287 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T109 14385-14390 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T110 14528-14535 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T111 14547-14551 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T112 14722-14725 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T113 14842-14845 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T114 14919-14922 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T115 15116-15123 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T116 15172-15179 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T117 15917-15924 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T118 16701-16711 Body_part denotes amino acid http://purl.org/sig/ont/fma/fma82739
T119 16947-16955 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T120 17043-17050 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257

LitCovid_AGAC

Id Subject Object Predicate Lexical cue
p35702s4 3176-3183 NegReg denotes reduced
p35702s6 3188-3207 MPA denotes oligomeric tendency
p35702s9 3211-3216 MPA denotes N-NTD
p35813s5 11620-11627 NegReg denotes delayed
p35813s6 11628-11658 MPA denotes N protein thermal denaturation
p35853s3 15094-15098 Protein denotes MERS
p35853s7 15116-15123 MPA denotes protein
p35853s8 15124-15133 NegReg denotes decreased
p35869s25 17021-17033 MPA denotes conformation

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T21 120-123 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T23 189-192 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T25 400-403 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T27 2853-2856 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T29 2915-2918 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T31 3123-3126 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T33 3213-3216 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T35 3432-3435 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T37 3865-3868 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T39 3910-3913 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T41 5542-5545 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T43 5914-5917 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T45 6135-6138 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T47 6731-6734 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T49 7094-7097 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T51 7224-7227 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T53 7336-7339 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T55 8105-8108 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T57 9702-9705 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T59 10688-10691 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T61 11079-11082 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T63 11370-11373 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T65 11828-11831 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T67 12609-12612 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T69 14984-14986 Disease denotes SE http://purl.obolibrary.org/obo/MONDO_0002125
T70 15318-15321 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T72 15400-15403 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T74 15539-15542 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T76 15870-15873 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T78 16683-16686 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T80 17674-17677 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T82 17841-17844 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T84 17923-17926 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T86 18454-18457 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T88 18776-18779 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T90 19156-19159 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T31 150-152 http://purl.obolibrary.org/obo/CLO_0007860 denotes MR
T32 328-329 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T33 348-349 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T34 357-359 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T35 479-480 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T36 512-513 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T37 536-537 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T38 574-575 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T39 628-629 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T40 702-703 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T41 939-940 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T42 1443-1444 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T43 1542-1543 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T44 1807-1811 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T45 1812-1813 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T46 1830-1831 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T47 2018-2022 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T48 2054-2055 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T49 2114-2115 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T50 2176-2180 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T51 2181-2182 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T52 2199-2200 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T53 2300-2302 http://purl.obolibrary.org/obo/CLO_0001562 denotes Å2
T54 2300-2302 http://purl.obolibrary.org/obo/CLO_0001563 denotes Å2
T55 2430-2431 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T56 2672-2675 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T57 2923-2924 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T58 3450-3452 http://purl.obolibrary.org/obo/CLO_0050509 denotes 27
T59 3464-3465 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T60 3871-3872 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T61 3963-3964 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T62 4091-4097 http://purl.obolibrary.org/obo/CLO_0001869 denotes B). (B
T63 4378-4385 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T64 4592-4593 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T65 5023-5030 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T66 5454-5455 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T67 5738-5740 http://purl.obolibrary.org/obo/CLO_0001236 denotes 2A
T68 5749-5750 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T69 5804-5806 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T70 5804-5806 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T71 6255-6260 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T72 6453-6455 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T73 6564-6566 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T74 6572-6573 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T75 6594-6595 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T76 6640-6641 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T77 6678-6679 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T78 6979-6981 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T79 6985-6987 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T80 7156-7158 http://purl.obolibrary.org/obo/CLO_0053799 denotes 45
T81 7480-7493 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T82 7544-7545 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T83 7599-7605 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T84 7715-7716 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T85 7763-7764 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T86 8020-8021 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T87 8091-8093 http://purl.obolibrary.org/obo/CLO_0003358 denotes FL
T88 8131-8133 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T89 8158-8159 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T90 8367-8369 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T91 8389-8391 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T92 8434-8435 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T93 8480-8481 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T94 8929-8930 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T95 8949-8950 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T96 9133-9136 http://purl.obolibrary.org/obo/CLO_0051456 denotes A–E
T97 9192-9193 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T98 9335-9336 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T99 9427-9428 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T100 9471-9472 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T101 9817-9819 http://purl.obolibrary.org/obo/CLO_0003358 denotes FL
T102 9898-9899 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T103 10051-10059 http://purl.obolibrary.org/obo/OBI_0000245 denotes organize
T104 10051-10059 http://purl.obolibrary.org/obo/OBI_0100026 denotes organize
T105 10051-10059 http://purl.obolibrary.org/obo/UBERON_0000468 denotes organize
T106 10063-10064 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T107 10823-10846 http://purl.obolibrary.org/obo/GO_0043234 denotes protein and its complex
T108 11047-11048 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T109 11259-11260 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T110 11286-11287 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T111 11340-11341 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T112 11479-11480 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T113 12109-12114 http://purl.obolibrary.org/obo/UBERON_0001456 denotes faces
T114 12240-12245 http://purl.obolibrary.org/obo/UBERON_0001456 denotes faces
T115 12738-12739 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T116 12835-12836 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T117 13213-13214 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T118 13269-13277 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T119 13291-13295 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T120 13425-13438 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T121 13500-13505 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T122 13548-13550 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T123 13606-13611 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T124 13663-13665 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T125 13813-13818 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T126 13916-13918 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4C
T127 13984-13990 http://purl.obolibrary.org/obo/SO_0000418 denotes signal
T128 14003-14016 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T129 14034-14038 http://purl.obolibrary.org/obo/CLO_0007842 denotes M P3
T130 14105-14110 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T131 14151-14156 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T132 14167-14168 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T133 14206-14213 http://purl.obolibrary.org/obo/SO_0000418 denotes signals
T134 14282-14287 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T135 14385-14390 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T136 14547-14551 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T137 14654-14655 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T138 14695-14696 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T139 14698-14699 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T140 14715-14716 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T141 14727-14728 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T142 14827-14829 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T143 15140-15142 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T144 15218-15219 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T145 15430-15432 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T146 15434-15436 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T147 15507-15509 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T148 15548-15550 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T149 15602-15603 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T150 15625-15627 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T151 16113-16115 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T152 16216-16218 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T153 16229-16230 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T154 16313-16315 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T155 16344-16345 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T156 16398-16400 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T157 16455-16456 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T158 16497-16499 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T159 16575-16577 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T160 16645-16646 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T161 16898-16899 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T162 17324-17325 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T163 17500-17501 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T164 17539-17540 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T165 17767-17777 http://purl.obolibrary.org/obo/CLO_0001658 denotes activities
T166 18355-18356 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T167 18363-18364 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T168 18462-18464 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T169 18466-18467 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T170 18469-18470 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T171 18497-18499 http://purl.obolibrary.org/obo/CLO_0003377 denotes Fo
T172 18500-18502 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T173 18535-18536 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T174 18581-18583 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T175 18684-18686 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T176 18706-18713 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T177 18737-18738 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T178 18753-18755 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T179 18810-18812 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T180 19087-19094 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T52 62-69 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T53 150-152 Chemical denotes MR http://purl.obolibrary.org/obo/CHEBI_74698
T54 198-200 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T55 404-413 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T56 877-885 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T57 1069-1076 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T58 1157-1167 Chemical denotes amino acid http://purl.obolibrary.org/obo/CHEBI_33709
T59 1157-1162 Chemical denotes amino http://purl.obolibrary.org/obo/CHEBI_46882
T60 1163-1167 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527
T61 1362-1369 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T62 1579-1582 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T63 1760-1768 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T64 1955-1963 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T65 2116-2124 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T66 2150-2156 Chemical denotes oxygen http://purl.obolibrary.org/obo/CHEBI_25805
T67 2570-2573 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T68 2757-2764 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T69 2898-2906 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T70 2949-2957 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T71 3441-3443 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T72 3780-3783 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T74 4796-4803 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T75 5145-5153 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T76 5565-5568 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T77 5804-5806 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T78 6121-6128 Chemical denotes ligands http://purl.obolibrary.org/obo/CHEBI_52214
T79 6184-6186 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T80 6238-6242 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T81 6415-6428 Chemical denotes hydroxymethyl http://purl.obolibrary.org/obo/CHEBI_24712
T82 6453-6455 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T83 6496-6498 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T84 6549-6553 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T85 6554-6562 Chemical denotes etodolac http://purl.obolibrary.org/obo/CHEBI_4909
T86 6564-6566 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T87 6585-6587 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T88 6830-6837 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T89 6966-6973 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T90 6979-6981 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T91 6985-6987 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T92 7272-7279 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T93 7584-7598 Chemical denotes lead compounds http://purl.obolibrary.org/obo/CHEBI_33585
T94 7661-7670 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T95 7724-7734 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T96 7753-7760 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T97 7833-7843 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T98 7874-7884 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T99 7954-7961 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T100 8009-8017 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T101 8091-8093 Chemical denotes FL http://purl.obolibrary.org/obo/CHEBI_91150
T102 8131-8133 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T103 8160-8166 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T104 8187-8191 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T105 8192-8195 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T106 8216-8220 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T107 8367-8369 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T108 8389-8391 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T109 8530-8532 Chemical denotes TI http://purl.obolibrary.org/obo/CHEBI_141446
T110 8556-8558 Chemical denotes NA http://purl.obolibrary.org/obo/CHEBI_33696
T111 8617-8624 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T112 8708-8715 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T113 8776-8783 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T114 8894-8896 Chemical denotes Rg http://purl.obolibrary.org/obo/CHEBI_33368
T115 8905-8912 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T116 8999-9006 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T117 9010-9018 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T118 9123-9130 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T119 9182-9189 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T120 9347-9354 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T121 9462-9469 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T122 9589-9596 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T123 9817-9819 Chemical denotes FL http://purl.obolibrary.org/obo/CHEBI_91150
T124 9846-9853 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T125 9900-9906 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T126 9927-9931 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T127 9932-9935 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T128 9944-9948 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T129 10042-10050 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T130 10270-10277 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T131 10527-10534 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T132 10752-10754 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T133 10823-10830 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T134 10953-10960 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T135 11032-11039 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T136 11101-11109 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T137 11147-11155 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T138 11223-11231 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T139 11439-11445 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T140 11553-11560 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T141 11630-11637 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T142 11738-11745 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T143 11881-11888 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T144 12670-12672 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T145 12943-12950 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T146 12951-12960 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T147 13080-13087 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T148 13137-13144 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T149 13713-13722 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T150 13761-13768 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T151 13963-13970 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T152 14076-14083 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T153 14124-14131 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T154 14198-14205 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T155 14253-14261 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T156 14528-14535 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T157 14666-14675 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T158 14984-14986 Chemical denotes SE http://purl.obolibrary.org/obo/CHEBI_74813
T159 15116-15123 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T160 15172-15179 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T161 15279-15283 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T162 15430-15432 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T163 15434-15436 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T164 15469-15475 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T165 15507-15509 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T166 15548-15550 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T167 15917-15924 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T168 16113-16115 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T169 16216-16218 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T170 16313-16315 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T171 16348-16353 Chemical denotes anion http://purl.obolibrary.org/obo/CHEBI_22563
T172 16378-16390 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T173 16378-16385 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T174 16398-16400 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T175 16401-16409 Chemical denotes indoline http://purl.obolibrary.org/obo/CHEBI_43295
T176 16459-16464 Chemical denotes donor http://purl.obolibrary.org/obo/CHEBI_17891
T177 16465-16473 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T178 16497-16499 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T179 16500-16512 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T180 16500-16507 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T181 16558-16560 Chemical denotes 5B http://purl.obolibrary.org/obo/CHEBI_27560
T182 16575-16577 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T183 16701-16711 Chemical denotes amino acid http://purl.obolibrary.org/obo/CHEBI_33709
T184 16701-16706 Chemical denotes amino http://purl.obolibrary.org/obo/CHEBI_46882
T185 16707-16711 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527
T186 16947-16955 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T187 16960-16967 Chemical denotes ligands http://purl.obolibrary.org/obo/CHEBI_52214
T188 17043-17050 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T189 17185-17197 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T190 17185-17192 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T191 17375-17381 Chemical denotes cation http://purl.obolibrary.org/obo/CHEBI_36916
T192 17757-17766 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T193 18317-18327 Chemical denotes apoprotein http://purl.obolibrary.org/obo/CHEBI_13850
T194 18462-18464 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T195 18497-18499 Chemical denotes Fo http://purl.obolibrary.org/obo/CHEBI_42485
T196 18581-18583 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T197 18684-18686 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T198 18753-18755 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T199 18810-18812 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T9 3133-3142 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T10 3327-3336 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T11 5931-5940 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T12 7272-7292 http://purl.obolibrary.org/obo/GO_0030164 denotes protein denaturation
T13 10416-10425 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T14 12003-12012 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T15 12995-13004 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T16 13056-13065 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T17 13574-13589 http://purl.obolibrary.org/obo/GO_0039703 denotes RNA replication
T18 14306-14315 http://purl.obolibrary.org/obo/GO_0009058 denotes formation

LitCovid-PD-HP

Id Subject Object Predicate Lexical cue hp_id
T3 6851-6859 Phenotype denotes rigidity http://purl.obolibrary.org/obo/HP_0002063

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T30 0-7 Sentence denotes Results
T31 9-69 Sentence denotes Structure of the N-Terminal Domain of the MERS-CoV N Protein
T32 70-201 Sentence denotes We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID:
T33 202-361 Sentence denotes 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1).
T34 362-515 Sentence denotes Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B).
T35 516-605 Sentence denotes The monomers shared a similar structural core preceded by a flexible region (Figure S1D).
T36 606-897 Sentence denotes The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent.
T37 898-1002 Sentence denotes Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric.
T38 1003-1083 Sentence denotes Figure 1B shows the details of the interactions in the MERS-CoV N protein dimer.
T39 1084-1139 Sentence denotes We named the units monomer 1 and monomer 2 (Figure 1A).
T40 1140-1370 Sentence denotes According to the amino acid composition of the binding site on monomer 2, we divided the dimeric interface into two areas: one located on the N-terminus flexible region and the other on the loop between β4 and β5 of the N protein.
T41 1371-1578 Sentence denotes In the first area, W43, N66, N68, Y102, and F135 of monomer 1 generated a conserved hydrophobic pocket permitting the side chain of M38 of monomer 2 to enter this hole by a hydrophobic contact (Figure 1C,D).
T42 1579-1712 Sentence denotes H37 and N39 of monomer 2 were packed against W43 and F135 of monomer 1, respectively, and contributed to the hydrophobic interaction.
T43 1713-1832 Sentence denotes The side chains of N39 of monomer 2 formed one hydrogen bond with the N68 backbone in monomer 1 at a distance of 2.6 Å.
T44 1833-1881 Sentence denotes The second area was relatively more hydrophilic.
T45 1882-2070 Sentence denotes The main chain oxygens of G104, F135, and T137 of monomer 2 formed three hydrogen bonds with the side chains of Q73 and T134 of monomer 1 at distances of 3.8, 3.2, and 3.7 Å, respectively.
T46 2071-2201 Sentence denotes The side chain of N139 on monomer 2 formed a hydrogen bond with the main chain oxygen of T137 on monomer 1 at a distance of 3.6 Å.
T47 2202-2317 Sentence denotes The interactions of the first and second areas comprise buried surface areas (BSA) of 289 and 103 Å2, respectively.
T48 2318-2465 Sentence denotes The small surface area buried at the interface accounts for ∼5 kcal mol–1 binding energy,26 which translates to a dissociation constant of ∼200 mM.
T49 2466-2614 Sentence denotes Thus, the dimer described here is unique in that it is non-native and relies on vector-fusion residues (H37 and M38) to maintain its dimeric status.
T50 2615-2907 Sentence denotes This property may also explain why the present structure has an oligomeric status different from previously reported structures for the CoV N protein.24,27−30 We used cross-linking experiments to analyze the oligomeric capacity of MERS N-NTD containing the vector-fusion residues in solution.
T51 2908-2958 Sentence denotes MERS N-NTD had a dimeric conformation in solution.
T52 2959-3143 Sentence denotes Our structure indicated that W43 played an essential role in forming the hydrophobic pocket accommodating the vector-fusion residues and, therefore, mediated the N-NTD dimer formation.
T53 3144-3230 Sentence denotes The W43A mutation significantly reduced the oligomeric tendency of N-NTD (Figure S1C).
T54 3231-3361 Sentence denotes This further supports that the “exogenous residues” encoded by the vector backbone mediated the formation of the non-native dimer.
T55 3362-3444 Sentence denotes We also superimposed the previously published structure of MERS-CoV N-NTD (PDB ID:
T56 3445-3525 Sentence denotes 4ud1)27 containing a native N-terminal flexible region with our dimer structure.
T57 3526-3671 Sentence denotes The side chain of N38 in the native structure could not interact with the hydrophobic pocket as the former is hydrophilic and short (Figure S1E).
T58 3672-3817 Sentence denotes Thus, it may be possible to utilize small compounds to replace the vector-fusion residues and stabilize the PPI through hydrophobic interactions.
T59 3818-4016 Sentence denotes Figure 1 Structure and sequence of MERS-CoV N-NTD. (A) Overall structure of the MERS-CoV N-NTD dimer containing monomers 1 and 2 is depicted as a cartoon and colored yellow and green, respectively.
T60 4017-4134 Sentence denotes Residues involved in dimerization are shown as sticks and highlighted in (B). (B) Interactions among MERS-CoV N-NTDs.
T61 4135-4332 Sentence denotes Dimerization is mediated mainly by vector-fusion residues interacting with the conserved hydrophobic regions on the core structure (first area) along with the residues surrounding it (second area).
T62 4333-4418 Sentence denotes Interacting residues on monomers 1 and 2 are labeled in black and blue, respectively.
T63 4419-4506 Sentence denotes Vector-fusion and conserved hydrophobic regions are colored cyan and red, respectively.
T64 4507-4595 Sentence denotes The color of all other interacting residues is the same as that for each monomer in (A).
T65 4596-4726 Sentence denotes Polar contacts are indicated with red dashed lines. (C) Upper panel: close-up of the interacting region of vector-fusion residues.
T66 4727-4812 Sentence denotes The surface was colored according to the hydrophobicity level at the protein surface.
T67 4813-4900 Sentence denotes Vector-fusion residues (black) are shown as sticks to emphasize the hydrophobic pocket.
T68 4901-4913 Sentence denotes Lower panel:
T69 4914-5007 Sentence denotes 2D diagrams of the interaction between the hydrophobic pocket and the vector-fusion residues.
T70 5008-5040 Sentence denotes The latter are labeled in black.
T71 5041-5179 Sentence denotes The hydrophobic contacts are indicated with black dashed lines. (D) Sequence alignment of various CoV N proteins in the N-terminal region.
T72 5180-5229 Sentence denotes Red letters indicate strictly conserved residues.
T73 5230-5277 Sentence denotes Cyan indicates conservative substitution sites.
T74 5278-5364 Sentence denotes Hydrophobic regions involved in unusual dimerization are indicated by black triangles.
T75 5366-5440 Sentence denotes Direct Targeting of the Non-native Dimer Interface for Antiviral Screening
T76 5441-5564 Sentence denotes We performed a structure-based virtual screening by targeting W43 in the hydrophobic pocket of the N-NTD dimeric interface.
T77 5565-5752 Sentence denotes H37 and M38 were removed from the template to identify compounds that could replace the vector-fusion residues and, therefore, contribute to the stabilizing effect (Figures 2A and S2A,B).
T78 5753-5918 Sentence denotes We chose the highest-scoring hits, listed in Table S2, based on shape complementarity, the presence of aromatic moieties, and the ability to stack onto W43 of N-NTD.
T79 5919-6320 Sentence denotes Because the formation of the non-native dimers was primarily mediated by hydrophobic interactions in our structure (Figure 1C,D), we next considered the hydrophobic complementarity between the acquired ligands and N-NTD in the form of the lipophilic match surface (SL/L).31 We also took into account the ability of the drug to permeate cells by aiming for lower topological polar surface areas (TPSA).
T80 6321-6404 Sentence denotes Based on the above criteria, three compounds were finally chosen for further study.
T81 6405-6535 Sentence denotes Benzyl-2-(hydroxymethyl)-1-indolinecarboxylate (P1) and 5-benzyloxygramine (P3) had higher SL/L and docking scores and lower TPSA.
T82 6536-6616 Sentence denotes The clinical drug etodolac (P2) had a comparable SL/L but a lower docking score.
T83 6617-6652 Sentence denotes It too was selected as a candidate.
T84 6653-7035 Sentence denotes However, only P3 induced a comparatively larger blue shift in the intrinsic N-NTD fluorescence spectrum, indicating that the microenvironment surrounding the tryptophans of the protein increased in rigidity and hydrophobicity in the presence of P3.32 The result also indicated that P3 bound more tightly to the N protein than P1 or P2 by interacting with the W43 pocket (Figure 2B).
T85 7036-7180 Sentence denotes Fluorescent thermal stability assays disclosed that the N-NTD denaturation melting temperature had increased from 42 to 45 °C when P3 was added.
T86 7181-7258 Sentence denotes The sigmoidal melting curve for MERS-CoV N-NTD changed in the presence of P3.
T87 7259-7368 Sentence denotes The delay in protein denaturation suggests that P3 stabilized the MERS-CoV N-NTD dimer structure (Figure 2C).
T88 7369-7517 Sentence denotes We then measured the cytotoxic concentration (CC50) and effective concentration (EC50) for each compound using Vero E6 cells infected with MERS-CoV.
T89 7518-7620 Sentence denotes Table 1 shows that P3 had a favorable therapeutic index among the lead compounds tested in this study.
T90 7621-7688 Sentence denotes Therefore, P3 is an excellent candidate inhibitor against MERS-CoV.
T91 7689-7858 Sentence denotes Figure 2 Compound P3 was a potent stabilizer of the MERS-CoV N protein. (A) Schematic depicting the rationale used in designing the allosteric stabilizer of this study.
T92 7859-8221 Sentence denotes An orthosteric stabilizer may be used to bind to the non-native interaction interface of the N protein and stabilize the abnormal interaction between proteins. (B) Conformation and (C) stability analyses were performed based on the FL spectra of NTD (1 μM) incubated with P1–P3 (10 μM) for 1 h with a buffer consisting of 50 mM Tris-HCl (pH 8.3) and 150 mM NaCl.
T93 8222-8286 Sentence denotes Table 1 CC50 and EC50 and Therapeutic Indexes of Lead Compounds
T94 8287-8326 Sentence denotes quantal dose–response relationship (μM)
T95 8327-8366 Sentence denotes compound CC50a EC50b TIc (CC50/EC50)
T96 8367-8388 Sentence denotes P1 459.69 >100 NAd
T97 8389-8410 Sentence denotes P2 569.77 >100 NAd
T98 8411-8433 Sentence denotes P3 805.32 32.1 25.1
T99 8434-8442 Sentence denotes a CC50:
T100 8443-8479 Sentence denotes Half maximal toxicity concentration.
T101 8480-8488 Sentence denotes b EC50:
T102 8489-8526 Sentence denotes Half maximum effective concentration.
T103 8527-8533 Sentence denotes c TI:
T104 8534-8552 Sentence denotes Therapeutic index.
T105 8553-8559 Sentence denotes d NA:
T106 8560-8573 Sentence denotes Nonavailable.
T107 8575-8636 Sentence denotes Structural Model of P3-Induced MERS-CoV N Protein Aggregation
T108 8637-8726 Sentence denotes We used SAXS to assess the effects of P3 on the full-length MERS-CoV N protein structure.
T109 8727-8827 Sentence denotes The fitted distance distribution function of the protein with and without P3 are shown in Figure 3A.
T110 8828-8965 Sentence denotes P3 increased the maximum dimension (Dmax) and radius of gyration (Rg) of the protein from 207 to 230 Å and from 58 to 65 Å, respectively.
T111 8966-9050 Sentence denotes Thus, the size of the MERS-CoV N protein in solution was altered upon binding to P3.
T112 9051-9289 Sentence denotes Figure 3 P3-induced abnormal aggregation on the full-length MERS-CoV N protein. (A–E) SAXS analysis of the full-length MERS-CoV N protein. (A) Normalized results from GNOM showing pairwise distance distribution P(r) and maximum distance.
T113 9290-9675 Sentence denotes The radius of gyration fitted to 207 and 230 Å for the N protein and the N-P3 complex, respectively. “r” represents pairwise distances. (B, C) Scattering profiles of the N protein (B) and the N-P3 complex (C) and normalization fitting with GNOM (dashed lines). (D, E) Representative models of the N protein (D) and the N-P3 complex (E) generated by CRYSOL simulations of the SAXS data.
T114 9676-9701 Sentence denotes Only α carbons are shown.
T115 9702-10004 Sentence denotes NTD (yellow), CTD (green), and disorder region (cyan). (F, G) Conformation (F) and stability (G) analyses based on FL spectra of the MERS-CoV N protein (1 μM) incubated with P3 (10 μM) for 1 h in a buffer consisting of 50 mM Tris-HCl, 150 mM NaCl (pH 8.3). (H) Schematic of the P3 inhibition mechanism.
T116 10005-10122 Sentence denotes Left panel: in the absence of RNA, N proteins organize as a dimeric building block contributed by N-CTD dimerization.
T117 10123-10299 Sentence denotes Middle panel: P3 promoted the dimerization of N-NTDs from different building blocks, by which the distance between CTD cuboids was shortened and N protein aggregation occurred.
T118 10300-10399 Sentence denotes Right panel: octameric conformation of building blocks buried in the RNA-binding surface of N-CTDs.
T119 10400-10459 Sentence denotes It hindered the formation of filamentous ribonucleocapsids.
T120 10460-10605 Sentence denotes The presence of multiple intrinsically disordered regions in the N protein precluded the determination of its structure by X-ray crystallography.
T121 10606-10755 Sentence denotes Instead, we used rigid body modeling of the SAXS data with the N-terminal domain (NTD; solved in this study) and the C-terminal domains (CTD, PDB ID:
T122 10756-10869 Sentence denotes 6G13).23 In this way, we obtained structural models for the free N protein and its complex with P3 (Figure 3B,C).
T123 10870-10899 Sentence denotes Excellent fits were obtained.
T124 10900-11020 Sentence denotes Representative structural models for the full-length protein without and with P3 are shown in Figure 3D,E, respectively.
T125 11021-11122 Sentence denotes The free N protein formed a tetramer through CTD with the NTD freely hanging in solution (Figure 3D).
T126 11123-11323 Sentence denotes The conformation of the solution was consistent with structures previously reported for other CoV N proteins.33 The N-P3 complex formed a compact hexadecamer with a sunburst configuration (Figure 3E).
T127 11324-11422 Sentence denotes The CTDs formed a central ring and non-native NTD dimers formed “spikes” protruding from the ring.
T128 11423-11595 Sentence denotes Consistent with ligand-induced aggregation, we observed a “blue shift” in the fluorescence spectrum of the full-length MERS-CoV N protein in the presence of P3 (Figure 3F).
T129 11596-11798 Sentence denotes The addition of P3 also delayed N protein thermal denaturation and changed the shape of the denaturation curve, further suggesting that large protein aggregates formed in the presence of P3 (Figure 3G).
T130 11799-11874 Sentence denotes The structure explains how N-NTD dimerization decreased MERS-CoV viability.
T131 11875-11935 Sentence denotes The N protein packages the viral genome into an RNP complex.
T132 11936-12195 Sentence denotes Several models for N-CTD dimer assembly have been proposed for the formation of filamentous RNPs.33 All of the proposed interfaces between N-CTD dimers occurred on the side-faces of the CTD cuboid perpendicular to the proposed RNA-binding surface (Figure 3H).
T133 12196-12487 Sentence denotes Combinatorial use of any region on the side-faces of the CTD dimer cuboid may facilitate manipulation of the RNP length and curvature without obstructing the RNA-binding surface.28,34 However, the SAXS results indicated that N-CTD aggregation occurred on the β-sheet floor of the CTD cuboid.
T134 12488-12674 Sentence denotes For this reason, the RNA-binding surface of the CTD is occluded by the neighboring CTD on the ring and by the non-native NTD dimer making direct contact with the CTD (Figures 3H and S3).
T135 12675-12858 Sentence denotes In addition, the CTD cuboids in the aggregation naturally form a topologically closed octamer, leaving no open ends for further addition of CTD cuboids to form a long filamentous RNP.
T136 12859-13016 Sentence denotes Both the loss of the RNA-binding surface and the inability to incorporate further N protein molecules beyond an octamer may inhibit the formation of the RNP.
T137 13017-13100 Sentence denotes Therefore, P3 may inhibit MERS-CoV RNP formation by inducing N protein aggregation.
T138 13102-13156 Sentence denotes P3 Inhibits MERS-CoV by Inducing N Protein Aggregation
T139 13157-13237 Sentence denotes We demonstrated that P3 had the best characteristics as a therapeutic candidate.
T140 13238-13492 Sentence denotes To determine the anti-MERS-CoV activity of P3 in the cell, the effects of P3 incubation on extracellular viral titers and intracellular viral RNA levels were assessed by plaque assays on Vero E6 cells (Figure 4A) and by RT-qPCR (Figure 4B), respectively.
T141 13493-13597 Sentence denotes At 50 μM, P3 marginally affected the viral titer after 48 h but suppressed viral RNA replication by 40%.
T142 13598-13668 Sentence denotes At 100 μM, P3 halted both viral production and replication after 48 h.
T143 13669-13732 Sentence denotes This result proved the capacity of P3 as an antiviral compound.
T144 13733-13877 Sentence denotes We then examined MERS-CoV N protein distribution and expression in the infected cells with or without P3 treatment to confirm our SAXS findings.
T145 13878-14039 Sentence denotes Immunofluorescence microscopy (Figure 4C) showed condensation of the intracellular N protein fluorescence signal in infected Vero E6 cells treated with 50 μM P3.
T146 14040-14096 Sentence denotes Thus, P3 may induce intracellular N protein aggregation.
T147 14097-14157 Sentence denotes At 100 μM, P3 suppressed N protein expression in most cells.
T148 14158-14214 Sentence denotes However, a few presented with intense N protein signals.
T149 14215-14358 Sentence denotes P3 may have restrained the MERS-CoV N proteins inside the infected cells that promoted the formation of new virions that could not be released.
T150 14359-14427 Sentence denotes In this way, the adjacent cells could not be infected with MERS-CoV.
T151 14428-14552 Sentence denotes The data, therefore, suggest that P3 may inhibit MERS-CoV by inducing abnormal aggregation of the N protein inside the cell.
T152 14553-14627 Sentence denotes This finding is consistent with the results of our structure-based assays.
T153 14628-14832 Sentence denotes Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h.
T154 14833-14912 Sentence denotes Relative RNA levels were determined by comparing MERS alone at each time point.
T155 14913-14948 Sentence denotes GAPDH RNA was the internal control.
T156 14949-15012 Sentence denotes All values are presented as mean ± SE (standard error of mean).
T157 15013-15158 Sentence denotes One-way Anova was used for statistics (*p < 0.05, **p < 0.01, ***p < 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment.
T158 15159-15248 Sentence denotes Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680.
T159 15249-15284 Sentence denotes Nuclei were stained blue with DAPI.
T160 15286-15353 Sentence denotes Crystal Structure of MERS-CoV N-NTD Complexed with Potent Compounds
T161 15354-15484 Sentence denotes We attempted to obtain crystals of MERS-CoV N-NTD in complex with compounds P1, P2, and P3 by cocrystallization or ligand-soaking.
T162 15485-15629 Sentence denotes With the exception of P2, the complex structures of N-NTD with P1 and P3 were solved at resolutions of 3.09 and 2.77 Å, respectively (Table S1).
T163 15630-15701 Sentence denotes The overall structures of the complexes resembled that of apo-MERS-CoV.
T164 15702-15885 Sentence denotes Both complexes revealed well-defined unbiased densities in the dimer interface and permitted detailed analysis of the interactions between the compounds and MERS-CoV N-NTD (Figure 5).
T165 15886-16008 Sentence denotes The interactions between the N protein and each compound were calculated with the Discovery Studio Client (v19.1.0.18287).
T166 16009-16105 Sentence denotes Most interactions were hydrophobic contacts, which were consistent with our selection rationale.
T167 16106-16249 Sentence denotes In the P1 complex, N68, F135, and D143 on monomer 1 and V41, G106, P107, and T137 on monomer 2 packed against P1 to create a dimer (Figure 5A).
T168 16250-16333 Sentence denotes In addition, two nonbonding interactions were detected between P1 and the monomers.
T169 16334-16439 Sentence denotes There was a π-anion interaction between the benzene ring of the P1 indoline moiety and D143 of monomer 1.
T170 16440-16562 Sentence denotes There was also a π-donor hydrogen bond between the other P1 benzene ring and the T137 side chain of monomer 2 (Figure 5B).
T171 16563-16696 Sentence denotes Relative to P1, P3 bound more deeply into the dimer interface and interacted with a larger number of residues on both N-NTD monomers.
T172 16697-16858 Sentence denotes The amino acid composition of this binding region was W43, N66, N68, S69, T70, N73, and F135 on monomer 1 and V41, G104, T105, G106, A109, and T137 on monomer 2.
T173 16859-17063 Sentence denotes These residues along with P3 generated a massive hydrophobic driving force allowing the proteins and ligands to pack against each other and stabilize the dimeric conformation of the N protein (Figure 5C).
T174 17064-17138 Sentence denotes Several nonbonding interactions were also observed at the P3-binding site.
T175 17139-17282 Sentence denotes These included the interaction between the P3 benzene ring and N68 of monomer 1 and A109 of monomer 2 via π-lone pair and π-alkyl interactions.
T176 17283-17366 Sentence denotes The dimethylaminomethyl moiety of P3 was a major source of nonbonding interactions.
T177 17367-17475 Sentence denotes Three π-cation interactions formed between this moiety and the aromatic groups of W43 and F135 in monomer 1.
T178 17476-17595 Sentence denotes This moiety also formed a π-lone pair interaction with N66 and a π-sigma interaction with W43 of monomer 1 (Figure 5D).
T179 17596-17805 Sentence denotes The structural analyses explain the comparatively stronger binding of P3 to N-NTD (Figure 2B) and corroborated the thermal stabilization effects (Figure 2C) and antiviral activities (Table 1) of the compounds.
T180 17806-17877 Sentence denotes Figure 5 Structures of MERS-CoV N-NTD complexed with potent compounds.
T181 17878-18366 Sentence denotes The structures were solved using HCoV-OC43 N-NTD (PDB:4J3K) as the search model.24 Left panel: (Upper) structural superimposition of the MERS-CoV N-NTD:P1 complex (monomers 1 and 2 are in purple and pink, respectively) and the MERS-CoV N-NTD:P3 complex (monomers 1 and 2 are in brown and green, respectively) with compounds depicted as stick structures. (Lower) Interactions involving vector-fusion residues in the non-native dimer of the apoprotein shown for comparison with (A) and (B).
T182 18367-18401 Sentence denotes Color is the same as in Figure 1A.
T183 18402-18486 Sentence denotes Right panel: detailed interactions among MERS-CoV N-NTD and P1 (A, B) and P3 (C, D).
T184 18487-18597 Sentence denotes Different Fo–Fc maps were contoured at ∼2.5 σ. (A) Detailed stereoview of interactions at the P1-binding site.
T185 18598-18657 Sentence denotes The color of each monomer is the same as in the left panel.
T186 18658-18780 Sentence denotes Residues constructing the P1-binding pocket are labeled and showed as sticks. (B) Schematic of P1 bound to MERS-CoV N-NTD.
T187 18781-18860 Sentence denotes Hydrophobic contacts between P1 and each monomer are displayed as dashed lines.
T188 18861-18978 Sentence denotes Nonbonding interactions are indicated by cyan arrows. (C) Detailed stereoview of interactions at the P3-binding site.
T189 18979-19038 Sentence denotes The color of each monomer is the same as in the left panel.
T190 19039-19160 Sentence denotes Residues belonging to the P3-binding pocket are labeled and shown as sticks. (D) Schematic of P3 bound to MERS-CoV N-NTD.
T191 19161-19240 Sentence denotes Hydrophobic contacts between P3 and each monomer are displayed as dashed lines.
T192 19241-19293 Sentence denotes Nonbonding interactions are indicated by red arrows.

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
87 51-59 Species denotes MERS-CoV Tax:1335626
93 109-117 Species denotes MERS-CoV Tax:1335626
94 177-186 Species denotes HCoV-OC43 Tax:31631
95 760-764 Species denotes CoVs Tax:11118
96 871-874 Species denotes CoV Tax:11118
97 398-403 Chemical denotes N-NTD
111 1343-1345 Gene denotes β4 Gene:28898
112 1350-1352 Gene denotes β5 Gene:28884
113 1058-1066 Species denotes MERS-CoV Tax:1335626
114 2751-2754 Species denotes CoV Tax:11118
115 3421-3429 Species denotes MERS-CoV Tax:1335626
116 1760-1768 Chemical denotes hydrogen MESH:D006859
117 1783-1786 Chemical denotes N68 MESH:C495502
118 1897-1904 Chemical denotes oxygens MESH:D010100
119 1955-1963 Chemical denotes hydrogen MESH:D006859
120 2089-2093 Chemical denotes N139
121 2116-2124 Chemical denotes hydrogen MESH:D006859
122 2150-2156 Chemical denotes oxygen MESH:D010100
123 3121-3126 Chemical denotes N-NTD
131 3931-3947 Gene denotes monomers 1 and 2
132 4357-4373 Gene denotes monomers 1 and 2
133 3854-3862 Species denotes MERS-CoV Tax:1335626
134 3899-3907 Species denotes MERS-CoV Tax:1335626
135 5139-5142 Species denotes CoV Tax:11118
136 3908-3913 Chemical denotes N-NTD
137 4123-4133 Disease denotes CoV N-NTDs MESH:C000657245
163 7213-7221 Species denotes MERS-CoV Tax:1335626
164 7325-7333 Species denotes MERS-CoV Tax:1335626
165 7508-7516 Species denotes MERS-CoV Tax:1335626
166 7679-7687 Species denotes MERS-CoV Tax:1335626
167 5540-5545 Chemical denotes N-NTD
168 5912-5917 Chemical denotes N-NTD
169 6133-6138 Chemical denotes N-NTD
170 6405-6451 Chemical denotes Benzyl-2-(hydroxymethyl)-1-indolinecarboxylate
171 6453-6455 Chemical denotes P1 MESH:C480041
172 6461-6479 Chemical denotes 5-benzyloxygramine MESH:C006736
173 6481-6483 Chemical denotes P3
174 6554-6562 Chemical denotes etodolac MESH:D017308
175 6564-6566 Chemical denotes P2 MESH:C020845
176 6667-6669 Chemical denotes P3
177 6729-6734 Chemical denotes N-NTD
178 6811-6822 Chemical denotes tryptophans MESH:D014364
179 6898-6900 Chemical denotes P3
180 7092-7097 Chemical denotes N-NTD
181 7167-7169 Chemical denotes P3
182 7255-7257 Chemical denotes P3
183 7307-7309 Chemical denotes P3
184 7632-7634 Chemical denotes P3
185 6851-6859 Disease denotes rigidity MESH:D009127
186 7494-7502 Disease denotes infected MESH:D007239
187 7485-7487 CellLine denotes E6 CVCL:4582
193 7742-7750 Species denotes MERS-CoV Tax:1335626
194 7708-7710 Chemical denotes P3
195 8131-8136 Chemical denotes P1–P3
196 8187-8195 Chemical denotes Tris-HCl
197 8216-8220 Chemical denotes NaCl MESH:D012965
201 8323-8325 Gene denotes μM
202 8385-8391 Chemical denotes NAd P2
203 8407-8413 Chemical denotes NAd P3
205 8456-8464 Disease denotes toxicity MESH:D064420
208 8595-8597 Chemical denotes P3
209 8606-8636 Disease denotes MERS-CoV N Protein Aggregation MESH:C000657245
214 8697-8705 Species denotes MERS-CoV Tax:1335626
215 8988-8996 Species denotes MERS-CoV Tax:1335626
216 8801-8803 Chemical denotes P3
217 9047-9049 Chemical denotes P3
233 9112-9120 Species denotes MERS-CoV Tax:1335626
234 9171-9179 Species denotes MERS-CoV Tax:1335626
235 9835-9843 Species denotes MERS-CoV Tax:1335626
236 9061-9063 Chemical denotes P3
237 9363-9367 Chemical denotes N-P3
238 9482-9486 Chemical denotes N-P3
239 9609-9613 Chemical denotes N-P3
240 9683-9690 Chemical denotes carbons MESH:D002244
241 9876-9878 Chemical denotes P3
242 9927-9935 Chemical denotes Tris-HCl
243 9944-9948 Chemical denotes NaCl MESH:D012965
244 10137-10139 Chemical denotes P3
245 10169-10175 Chemical denotes N-NTDs
246 10392-10398 Chemical denotes N-CTDs
247 9072-9092 Disease denotes abnormal aggregation MESH:D001791
264 11923-11926 Gene denotes RNP Gene:55599
265 13052-13055 Gene denotes RNP Gene:55599
266 13012-13015 Gene denotes RNP Gene:55599
267 12854-12857 Gene denotes RNP Gene:55599
268 12305-12308 Gene denotes RNP Gene:55599
269 11217-11220 Species denotes CoV Tax:11118
270 11542-11550 Species denotes MERS-CoV Tax:1335626
271 11855-11863 Species denotes MERS-CoV Tax:1335626
272 13043-13051 Species denotes MERS-CoV Tax:1335626
273 11239-11243 Chemical denotes N-P3
274 11612-11614 Chemical denotes P3
275 11783-11785 Chemical denotes P3
276 11826-11831 Chemical denotes N-NTD
277 11955-11960 Chemical denotes N-CTD
278 10880-10884 Disease denotes fits MESH:D012640
279 12421-12438 Disease denotes N-CTD aggregation MESH:D001791
281 13114-13122 Species denotes MERS-CoV Tax:1335626
299 13750-13758 Species denotes MERS-CoV Tax:1335626
300 14242-14250 Species denotes MERS-CoV Tax:1335626
301 14418-14426 Species denotes MERS-CoV Tax:1335626
302 14477-14485 Species denotes MERS-CoV Tax:1335626
303 13260-13268 Species denotes MERS-CoV Tax:1335626
304 13178-13180 Chemical denotes P3
305 13609-13611 Chemical denotes P3
306 13704-13706 Chemical denotes P3
307 14036-14038 Chemical denotes P3
308 14046-14048 Chemical denotes P3
309 13804-13812 Disease denotes infected MESH:D007239
310 13994-14002 Disease denotes infected MESH:D007239
311 14273-14281 Disease denotes infected MESH:D007239
312 14404-14412 Disease denotes infected MESH:D007239
313 14498-14518 Disease denotes abnormal aggregation MESH:D001791
314 13430-13432 CellLine denotes E6 CVCL:4582
315 14008-14010 CellLine denotes E6 CVCL:4582
322 14913-14918 Gene denotes GAPDH Gene:2597
323 14684-14692 Species denotes MERS-CoV Tax:1335626
324 14733-14741 Species denotes MERS-CoV Tax:1335626
325 15094-15102 Species denotes MERS-CoV Tax:1335626
326 15145-15147 Chemical denotes P3
327 15279-15283 Chemical denotes DAPI MESH:C007293
330 15307-15315 Species denotes MERS-CoV Tax:1335626
331 15317-15321 Chemical denotes -NTD
352 15389-15397 Species denotes MERS-CoV Tax:1335626
353 15692-15700 Species denotes MERS-CoV Tax:1335626
354 15859-15867 Species denotes MERS-CoV Tax:1335626
355 15537-15542 Chemical denotes N-NTD
356 16378-16385 Chemical denotes benzene MESH:D001554
357 16401-16409 Chemical denotes indoline MESH:C057812
358 16421-16425 Chemical denotes D143
359 16465-16473 Chemical denotes hydrogen MESH:D006859
360 16500-16507 Chemical denotes benzene MESH:D001554
361 16575-16577 Chemical denotes P1 MESH:C480041
362 16579-16581 Chemical denotes P3
363 16681-16686 Chemical denotes N-NTD
364 16885-16887 Chemical denotes P3
365 17185-17192 Chemical denotes benzene MESH:D001554
366 17202-17205 Chemical denotes N68 MESH:C495502
367 17223-17227 Chemical denotes A109
368 17287-17306 Chemical denotes dimethylaminomethyl
369 17317-17319 Chemical denotes P3
370 17666-17668 Chemical denotes P3
371 17672-17677 Chemical denotes N-NTD
386 18030-18058 Gene denotes P1 complex (monomers 1 and 2
387 18132-18148 Gene denotes monomers 1 and 2
388 17830-17838 Species denotes MERS-CoV Tax:1335626
389 17911-17920 Species denotes HCoV-OC43 Tax:31631
390 18015-18023 Species denotes MERS-CoV Tax:1335626
391 18105-18113 Species denotes MERS-CoV Tax:1335626
392 18443-18451 Species denotes MERS-CoV Tax:1335626
393 18765-18773 Species denotes MERS-CoV Tax:1335626
394 19145-19153 Species denotes MERS-CoV Tax:1335626
395 17839-17844 Chemical denotes N-NTD
396 17921-17926 Chemical denotes N-NTD
397 18120-18122 Chemical denotes P3
398 18452-18457 Chemical denotes N-NTD
399 19133-19135 Chemical denotes P3

2_test

Id Subject Object Predicate Lexical cue
32105468-16338414-61929504 765-767 16338414 denotes 25
32105468-23389845-61929505 2407-2409 23389845 denotes 26
32105468-26894667-61929506 2768-2770 26894667 denotes 27
32105468-19782089-61929507 2771-2773 19782089 denotes 30
32105468-26894667-61929508 3450-3452 26894667 denotes 27
32105468-19244385-61929509 6190-6192 19244385 denotes 31
32105468-30644840-61929510 10762-10764 30644840 denotes 23
32105468-28044277-61929511 11232-11234 28044277 denotes 33
32105468-28044277-61929512 12033-12035 28044277 denotes 33
32105468-16775348-61929513 12374-12376 16775348 denotes 28
32105468-17379242-61929514 12377-12379 17379242 denotes 34