PMC:7094172 / 37633-38511 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T652","span":{"begin":25,"end":29},"obj":"CL_6"},{"id":"T651","span":{"begin":128,"end":136},"obj":"SP_9"},{"id":"T650","span":{"begin":269,"end":281},"obj":"CHEBI:9750;CHEBI:9750"},{"id":"T649","span":{"begin":371,"end":379},"obj":"SP_9"},{"id":"T648","span":{"begin":390,"end":398},"obj":"GO:0042571"},{"id":"T647","span":{"begin":476,"end":481},"obj":"NCBITaxon:10239"},{"id":"T646","span":{"begin":562,"end":576},"obj":"CHEBI:51766;CHEBI:51766"},{"id":"T645","span":{"begin":582,"end":588},"obj":"NCBITaxon:9986"},{"id":"T644","span":{"begin":599,"end":607},"obj":"GO:0042571"},{"id":"T643","span":{"begin":694,"end":711},"obj":"CHEBI:51231;CHEBI:51231"},{"id":"T642","span":{"begin":711,"end":723},"obj":"CHEBI:48559;CHEBI:48559"},{"id":"T641","span":{"begin":725,"end":729},"obj":"CHEBI:51231;CHEBI:51231"},{"id":"T640","span":{"begin":762,"end":766},"obj":"SP_9"},{"id":"T639","span":{"begin":780,"end":790},"obj":"GO:0010467"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T169","span":{"begin":33,"end":38},"obj":"Body_part"},{"id":"T170","span":{"begin":158,"end":163},"obj":"Body_part"},{"id":"T171","span":{"begin":390,"end":398},"obj":"Body_part"},{"id":"T172","span":{"begin":487,"end":492},"obj":"Body_part"},{"id":"T173","span":{"begin":599,"end":607},"obj":"Body_part"},{"id":"T174","span":{"begin":853,"end":855},"obj":"Body_part"}],"attributes":[{"id":"A169","pred":"fma_id","subj":"T169","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A170","pred":"fma_id","subj":"T170","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A171","pred":"fma_id","subj":"T171","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A172","pred":"fma_id","subj":"T172","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A173","pred":"fma_id","subj":"T173","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A174","pred":"fma_id","subj":"T174","obj":"http://purl.org/sig/ont/fma/fma61898"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T123","span":{"begin":113,"end":122},"obj":"Disease"}],"attributes":[{"id":"A123","pred":"mondo_id","subj":"T123","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T281","span":{"begin":25,"end":38},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T282","span":{"begin":158,"end":163},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T283","span":{"begin":220,"end":224},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T284","span":{"begin":327,"end":328},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T285","span":{"begin":476,"end":481},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T286","span":{"begin":487,"end":492},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T287","span":{"begin":810,"end":811},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T351","span":{"begin":269,"end":281},"obj":"Chemical"},{"id":"T352","span":{"begin":338,"end":344},"obj":"Chemical"},{"id":"T353","span":{"begin":567,"end":572},"obj":"Chemical"},{"id":"T354","span":{"begin":661,"end":663},"obj":"Chemical"},{"id":"T357","span":{"begin":711,"end":723},"obj":"Chemical"},{"id":"T358","span":{"begin":725,"end":729},"obj":"Chemical"},{"id":"T359","span":{"begin":853,"end":855},"obj":"Chemical"}],"attributes":[{"id":"A351","pred":"chebi_id","subj":"T351","obj":"http://purl.obolibrary.org/obo/CHEBI_9750"},{"id":"A352","pred":"chebi_id","subj":"T352","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A353","pred":"chebi_id","subj":"T353","obj":"http://purl.obolibrary.org/obo/CHEBI_24061"},{"id":"A354","pred":"chebi_id","subj":"T354","obj":"http://purl.obolibrary.org/obo/CHEBI_474859"},{"id":"A355","pred":"chebi_id","subj":"T354","obj":"http://purl.obolibrary.org/obo/CHEBI_73610"},{"id":"A356","pred":"chebi_id","subj":"T354","obj":"http://purl.obolibrary.org/obo/CHEBI_90325"},{"id":"A357","pred":"chebi_id","subj":"T357","obj":"http://purl.obolibrary.org/obo/CHEBI_48559"},{"id":"A358","pred":"chebi_id","subj":"T358","obj":"http://purl.obolibrary.org/obo/CHEBI_51231"},{"id":"A359","pred":"chebi_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/CHEBI_27569"},{"id":"A360","pred":"chebi_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/CHEBI_40618"},{"id":"A361","pred":"chebi_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/CHEBI_73757"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T302","span":{"begin":0,"end":24},"obj":"Sentence"},{"id":"T303","span":{"begin":25,"end":153},"obj":"Sentence"},{"id":"T304","span":{"begin":154,"end":293},"obj":"Sentence"},{"id":"T305","span":{"begin":294,"end":365},"obj":"Sentence"},{"id":"T306","span":{"begin":366,"end":482},"obj":"Sentence"},{"id":"T307","span":{"begin":483,"end":693},"obj":"Sentence"},{"id":"T308","span":{"begin":694,"end":761},"obj":"Sentence"},{"id":"T309","span":{"begin":762,"end":878},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"615","span":{"begin":128,"end":136},"obj":"Species"},{"id":"616","span":{"begin":371,"end":379},"obj":"Species"},{"id":"617","span":{"begin":189,"end":205},"obj":"Chemical"},{"id":"618","span":{"begin":269,"end":281},"obj":"Chemical"},{"id":"619","span":{"begin":538,"end":541},"obj":"Chemical"},{"id":"620","span":{"begin":562,"end":576},"obj":"Chemical"},{"id":"621","span":{"begin":694,"end":723},"obj":"Chemical"},{"id":"622","span":{"begin":725,"end":729},"obj":"Chemical"},{"id":"623","span":{"begin":752,"end":755},"obj":"Chemical"},{"id":"624","span":{"begin":113,"end":122},"obj":"Disease"},{"id":"625","span":{"begin":30,"end":32},"obj":"CellLine"}],"attributes":[{"id":"A615","pred":"tao:has_database_id","subj":"615","obj":"Tax:1335626"},{"id":"A616","pred":"tao:has_database_id","subj":"616","obj":"Tax:1335626"},{"id":"A617","pred":"tao:has_database_id","subj":"617","obj":"MESH:C003043"},{"id":"A618","pred":"tao:has_database_id","subj":"618","obj":"MESH:D017830"},{"id":"A621","pred":"tao:has_database_id","subj":"621","obj":"MESH:C007293"},{"id":"A622","pred":"tao:has_database_id","subj":"622","obj":"MESH:C007293"},{"id":"A624","pred":"tao:has_database_id","subj":"624","obj":"MESH:D007239"},{"id":"A625","pred":"tao:has_database_id","subj":"625","obj":"CVCL:4582"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Immunofluorescence Assay\nVero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany)."}