PMC:7094172 / 37633-38511
Annnotations
LitCovid-PMC-OGER-BB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T652 | 25-29 | CL_6 | denotes | Vero |
| T651 | 128-136 | SP_9 | denotes | MERS-CoV |
| T650 | 269-281 | CHEBI:9750;CHEBI:9750 | denotes | Triton X-100 |
| T649 | 371-379 | SP_9 | denotes | MERS-CoV |
| T648 | 390-398 | GO:0042571 | denotes | antibody |
| T647 | 476-481 | NCBITaxon:10239 | denotes | virus |
| T646 | 562-576 | CHEBI:51766;CHEBI:51766 | denotes | Alex Fluor 568 |
| T645 | 582-588 | NCBITaxon:9986 | denotes | rabbit |
| T644 | 599-607 | GO:0042571 | denotes | antibody |
| T643 | 694-711 | CHEBI:51231;CHEBI:51231 | denotes | 4′,6-Diamidino-2- |
| T642 | 711-723 | CHEBI:48559;CHEBI:48559 | denotes | phenylindole |
| T641 | 725-729 | CHEBI:51231;CHEBI:51231 | denotes | DAPI |
| T640 | 762-766 | SP_9 | denotes | MERS |
| T639 | 780-790 | GO:0010467 | denotes | expression |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T169 | 33-38 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T170 | 158-163 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T171 | 390-398 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T172 | 487-492 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T173 | 599-607 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T174 | 853-855 | Body_part | denotes | AG | http://purl.org/sig/ont/fma/fma61898 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T123 | 113-122 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T281 | 25-38 | http://purl.obolibrary.org/obo/CLO_0051719 | denotes | Vero E6 cells |
| T282 | 158-163 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T283 | 220-224 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T284 | 327-328 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T285 | 476-481 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T286 | 487-492 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T287 | 810-811 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T351 | 269-281 | Chemical | denotes | Triton X-100 | http://purl.obolibrary.org/obo/CHEBI_9750 |
| T352 | 338-344 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T353 | 567-572 | Chemical | denotes | Fluor | http://purl.obolibrary.org/obo/CHEBI_24061 |
| T354 | 661-663 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
| T357 | 711-723 | Chemical | denotes | phenylindole | http://purl.obolibrary.org/obo/CHEBI_48559 |
| T358 | 725-729 | Chemical | denotes | DAPI | http://purl.obolibrary.org/obo/CHEBI_51231 |
| T359 | 853-855 | Chemical | denotes | AG | http://purl.obolibrary.org/obo/CHEBI_27569|http://purl.obolibrary.org/obo/CHEBI_40618|http://purl.obolibrary.org/obo/CHEBI_73757 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T302 | 0-24 | Sentence | denotes | Immunofluorescence Assay |
| T303 | 25-153 | Sentence | denotes | Vero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1. |
| T304 | 154-293 | Sentence | denotes | The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min. |
| T305 | 294-365 | Sentence | denotes | Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C. |
| T306 | 366-482 | Sentence | denotes | Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus. |
| T307 | 483-693 | Sentence | denotes | The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature. |
| T308 | 694-761 | Sentence | denotes | 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash. |
| T309 | 762-878 | Sentence | denotes | MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany). |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 615 | 128-136 | Species | denotes | MERS-CoV | Tax:1335626 |
| 616 | 371-379 | Species | denotes | MERS-CoV | Tax:1335626 |
| 617 | 189-205 | Chemical | denotes | paraformaldehyde | MESH:C003043 |
| 618 | 269-281 | Chemical | denotes | Triton X-100 | MESH:D017830 |
| 619 | 538-541 | Chemical | denotes | PBS | |
| 620 | 562-576 | Chemical | denotes | Alex Fluor 568 | |
| 621 | 694-723 | Chemical | denotes | 4′,6-Diamidino-2-phenylindole | MESH:C007293 |
| 622 | 725-729 | Chemical | denotes | DAPI | MESH:C007293 |
| 623 | 752-755 | Chemical | denotes | PBS | |
| 624 | 113-122 | Disease | denotes | infection | MESH:D007239 |
| 625 | 30-32 | CellLine | denotes | E6 | CVCL:4582 |