PMC:7094172 / 29849-30853
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7094172","sourcedb":"PMC","sourceid":"7094172","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7094172","text":"MERS-CoV N-NTD crystals were grown as previously described:46 MERS-CoV N-NTD was crystallized at room temperature (∼25 °C) by the sitting-drop vapor-diffusion method. A protein solution (2 μL; 10 mg mL–1) was mixed with an equal volume of crystallization solution consisting of 75 mM ammonium sulfate, 2 mM NaBr, and 29% PEG 3350 (Sigma-Aldrich Corp., St. Louis, MO) and equilibrated against a 300 μL solution. MERS-CoV N-NTD:P3 co-crystals were obtained using a crystallization solution containing 2 mM P3. MERS-CoV N-NTD crystals in complex with P1 were obtained by soaking native MERS-CoV N-NTD crystals for 90 s at room temperature in a crystallization solution containing 2 mM P1. Diffraction datasets for MERS-CoV N-NTD alone and in complex with P1 were collected at beamline 13B1 of the Taiwan Light Source (TLS) of the National Synchrotron Research Center (NSRRC; Hsinchu City, Taiwan). Diffraction of the MERS-CoV N-NTD:P3 complex was performed at the beamline SP44XU of SPring-8 (Hyogo, Japan).","tracks":[{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T496","span":{"begin":0,"end":8},"obj":"SP_9"},{"id":"T495","span":{"begin":29,"end":34},"obj":"GO:0040007"},{"id":"T494","span":{"begin":62,"end":70},"obj":"SP_9"},{"id":"T493","span":{"begin":177,"end":185},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T492","span":{"begin":255,"end":263},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T491","span":{"begin":284,"end":300},"obj":"CHEBI:62946;CHEBI:62946"},{"id":"T490","span":{"begin":307,"end":311},"obj":"CHEBI:26710;CHEBI:26710"},{"id":"T489","span":{"begin":321,"end":324},"obj":"CHEBI:53250;CHEBI:53250"},{"id":"T488","span":{"begin":401,"end":409},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T487","span":{"begin":411,"end":419},"obj":"SP_9"},{"id":"T486","span":{"begin":479,"end":487},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T485","span":{"begin":508,"end":516},"obj":"SP_9"},{"id":"T484","span":{"begin":535,"end":542},"obj":"GO:1990524"},{"id":"T483","span":{"begin":583,"end":591},"obj":"SP_9"},{"id":"T482","span":{"begin":657,"end":665},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T481","span":{"begin":711,"end":719},"obj":"SP_9"},{"id":"T480","span":{"begin":739,"end":746},"obj":"GO:1990524"},{"id":"T479","span":{"begin":914,"end":922},"obj":"SP_9"},{"id":"T478","span":{"begin":932,"end":939},"obj":"GO:0032991"}],"attributes":[{"subj":"T496","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T495","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T494","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T493","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T492","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T491","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T490","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T489","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T488","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T487","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T486","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T485","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T484","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T483","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T482","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T481","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T480","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T479","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T478","pred":"source","obj":"LitCovid-PMC-OGER-BB"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T144","span":{"begin":169,"end":176},"obj":"Body_part"}],"attributes":[{"id":"A144","pred":"fma_id","subj":"T144","obj":"http://purl.org/sig/ont/fma/fma67257"},{"subj":"T144","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T99","span":{"begin":11,"end":14},"obj":"Disease"},{"id":"T101","span":{"begin":73,"end":76},"obj":"Disease"},{"id":"T103","span":{"begin":519,"end":522},"obj":"Disease"},{"id":"T105","span":{"begin":594,"end":597},"obj":"Disease"},{"id":"T107","span":{"begin":722,"end":725},"obj":"Disease"}],"attributes":[{"id":"A99","pred":"mondo_id","subj":"T99","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A100","pred":"mondo_id","subj":"T99","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A101","pred":"mondo_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A102","pred":"mondo_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A103","pred":"mondo_id","subj":"T103","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A104","pred":"mondo_id","subj":"T103","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A105","pred":"mondo_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A106","pred":"mondo_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A107","pred":"mondo_id","subj":"T107","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A108","pred":"mondo_id","subj":"T107","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"subj":"T99","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T101","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T103","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T105","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T107","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T222","span":{"begin":167,"end":168},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T223","span":{"begin":199,"end":203},"obj":"http://purl.obolibrary.org/obo/CLO_0007735"},{"id":"T224","span":{"begin":352,"end":354},"obj":"http://purl.obolibrary.org/obo/CLO_0009141"},{"id":"T225","span":{"begin":352,"end":354},"obj":"http://purl.obolibrary.org/obo/CLO_0050980"},{"id":"T226","span":{"begin":363,"end":365},"obj":"http://purl.obolibrary.org/obo/CLO_0007815"},{"id":"T227","span":{"begin":392,"end":393},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T228","span":{"begin":461,"end":462},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T229","span":{"begin":548,"end":550},"obj":"http://purl.obolibrary.org/obo/CLO_0008285"},{"id":"T230","span":{"begin":639,"end":640},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T231","span":{"begin":682,"end":684},"obj":"http://purl.obolibrary.org/obo/CLO_0008285"},{"id":"T232","span":{"begin":752,"end":754},"obj":"http://purl.obolibrary.org/obo/CLO_0008285"}],"attributes":[{"subj":"T222","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T223","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T224","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T225","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T226","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T227","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T228","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T229","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T230","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T231","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T232","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T265","span":{"begin":169,"end":176},"obj":"Chemical"},{"id":"T266","span":{"begin":177,"end":185},"obj":"Chemical"},{"id":"T267","span":{"begin":255,"end":263},"obj":"Chemical"},{"id":"T268","span":{"begin":284,"end":300},"obj":"Chemical"},{"id":"T269","span":{"begin":284,"end":292},"obj":"Chemical"},{"id":"T270","span":{"begin":293,"end":300},"obj":"Chemical"},{"id":"T271","span":{"begin":307,"end":311},"obj":"Chemical"},{"id":"T272","span":{"begin":321,"end":324},"obj":"Chemical"},{"id":"T273","span":{"begin":401,"end":409},"obj":"Chemical"},{"id":"T274","span":{"begin":479,"end":487},"obj":"Chemical"},{"id":"T275","span":{"begin":548,"end":550},"obj":"Chemical"},{"id":"T276","span":{"begin":657,"end":665},"obj":"Chemical"},{"id":"T277","span":{"begin":682,"end":684},"obj":"Chemical"},{"id":"T278","span":{"begin":752,"end":754},"obj":"Chemical"},{"id":"T279","span":{"begin":801,"end":806},"obj":"Chemical"}],"attributes":[{"id":"A265","pred":"chebi_id","subj":"T265","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A266","pred":"chebi_id","subj":"T266","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A267","pred":"chebi_id","subj":"T267","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A268","pred":"chebi_id","subj":"T268","obj":"http://purl.obolibrary.org/obo/CHEBI_62946"},{"id":"A269","pred":"chebi_id","subj":"T269","obj":"http://purl.obolibrary.org/obo/CHEBI_28938"},{"id":"A270","pred":"chebi_id","subj":"T270","obj":"http://purl.obolibrary.org/obo/CHEBI_16189"},{"id":"A271","pred":"chebi_id","subj":"T271","obj":"http://purl.obolibrary.org/obo/CHEBI_63004"},{"id":"A272","pred":"chebi_id","subj":"T272","obj":"http://purl.obolibrary.org/obo/CHEBI_46793"},{"id":"A273","pred":"chebi_id","subj":"T273","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A274","pred":"chebi_id","subj":"T274","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A275","pred":"chebi_id","subj":"T275","obj":"http://purl.obolibrary.org/obo/CHEBI_60949"},{"id":"A276","pred":"chebi_id","subj":"T276","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A277","pred":"chebi_id","subj":"T277","obj":"http://purl.obolibrary.org/obo/CHEBI_60949"},{"id":"A278","pred":"chebi_id","subj":"T278","obj":"http://purl.obolibrary.org/obo/CHEBI_60949"},{"id":"A279","pred":"chebi_id","subj":"T279","obj":"http://purl.obolibrary.org/obo/CHEBI_30212"},{"subj":"T265","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T266","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T267","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T268","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T269","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T270","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T271","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T272","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T273","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T274","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T275","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T276","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T277","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T278","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T279","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T229","span":{"begin":0,"end":166},"obj":"Sentence"},{"id":"T230","span":{"begin":167,"end":410},"obj":"Sentence"},{"id":"T231","span":{"begin":411,"end":507},"obj":"Sentence"},{"id":"T232","span":{"begin":508,"end":685},"obj":"Sentence"},{"id":"T233","span":{"begin":686,"end":894},"obj":"Sentence"},{"id":"T234","span":{"begin":895,"end":1004},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T229","pred":"source","obj":"LitCovid-sentences"},{"subj":"T230","pred":"source","obj":"LitCovid-sentences"},{"subj":"T231","pred":"source","obj":"LitCovid-sentences"},{"subj":"T232","pred":"source","obj":"LitCovid-sentences"},{"subj":"T233","pred":"source","obj":"LitCovid-sentences"},{"subj":"T234","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"470","span":{"begin":199,"end":203},"obj":"Gene"},{"id":"471","span":{"begin":970,"end":988},"obj":"Gene"},{"id":"472","span":{"begin":0,"end":8},"obj":"Species"},{"id":"473","span":{"begin":62,"end":70},"obj":"Species"},{"id":"474","span":{"begin":411,"end":419},"obj":"Species"},{"id":"475","span":{"begin":508,"end":516},"obj":"Species"},{"id":"476","span":{"begin":583,"end":591},"obj":"Species"},{"id":"477","span":{"begin":711,"end":719},"obj":"Species"},{"id":"478","span":{"begin":914,"end":922},"obj":"Species"},{"id":"479","span":{"begin":284,"end":300},"obj":"Chemical"},{"id":"480","span":{"begin":307,"end":311},"obj":"Chemical"},{"id":"481","span":{"begin":321,"end":324},"obj":"Chemical"},{"id":"482","span":{"begin":426,"end":428},"obj":"Chemical"},{"id":"483","span":{"begin":504,"end":506},"obj":"Chemical"},{"id":"484","span":{"begin":923,"end":928},"obj":"Chemical"},{"id":"485","span":{"begin":929,"end":931},"obj":"Chemical"}],"attributes":[{"id":"A470","pred":"tao:has_database_id","subj":"470","obj":"Gene:23961"},{"id":"A472","pred":"tao:has_database_id","subj":"472","obj":"Tax:1335626"},{"id":"A473","pred":"tao:has_database_id","subj":"473","obj":"Tax:1335626"},{"id":"A474","pred":"tao:has_database_id","subj":"474","obj":"Tax:1335626"},{"id":"A475","pred":"tao:has_database_id","subj":"475","obj":"Tax:1335626"},{"id":"A476","pred":"tao:has_database_id","subj":"476","obj":"Tax:1335626"},{"id":"A477","pred":"tao:has_database_id","subj":"477","obj":"Tax:1335626"},{"id":"A478","pred":"tao:has_database_id","subj":"478","obj":"Tax:1335626"},{"id":"A479","pred":"tao:has_database_id","subj":"479","obj":"MESH:D000645"},{"subj":"470","pred":"source","obj":"LitCovid-PubTator"},{"subj":"471","pred":"source","obj":"LitCovid-PubTator"},{"subj":"472","pred":"source","obj":"LitCovid-PubTator"},{"subj":"473","pred":"source","obj":"LitCovid-PubTator"},{"subj":"474","pred":"source","obj":"LitCovid-PubTator"},{"subj":"475","pred":"source","obj":"LitCovid-PubTator"},{"subj":"476","pred":"source","obj":"LitCovid-PubTator"},{"subj":"477","pred":"source","obj":"LitCovid-PubTator"},{"subj":"478","pred":"source","obj":"LitCovid-PubTator"},{"subj":"479","pred":"source","obj":"LitCovid-PubTator"},{"subj":"480","pred":"source","obj":"LitCovid-PubTator"},{"subj":"481","pred":"source","obj":"LitCovid-PubTator"},{"subj":"482","pred":"source","obj":"LitCovid-PubTator"},{"subj":"483","pred":"source","obj":"LitCovid-PubTator"},{"subj":"484","pred":"source","obj":"LitCovid-PubTator"},{"subj":"485","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"2_test","denotations":[{"id":"32105468-26249685-61929525","span":{"begin":59,"end":61},"obj":"26249685"}],"attributes":[{"subj":"32105468-26249685-61929525","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PMC-OGER-BB","color":"#ecbe93","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#a493ec"},{"id":"LitCovid-PD-MONDO","color":"#9cec93"},{"id":"LitCovid-PD-CLO","color":"#ec93b6"},{"id":"LitCovid-PD-CHEBI","color":"#93d0ec"},{"id":"LitCovid-sentences","color":"#eaec93"},{"id":"LitCovid-PubTator","color":"#d493ec"},{"id":"2_test","color":"#93ecba"}]}]}}