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LitCovid-PMC-OGER-BB

Id Subject Object Predicate Lexical cue
T760 22583-22591 SP_9 denotes MERS-CoV
T759 22598-22607 GO:0032991 denotes complexed
T758 22620-22629 CHEBI:36357;CHEBI:36357 denotes compounds
T757 22768-22776 SP_9 denotes MERS-CoV
T756 22786-22793 GO:0032991 denotes complex
T755 22858-22866 SP_9 denotes MERS-CoV
T754 22876-22883 GO:0032991 denotes complex
T753 22945-22954 CHEBI:36357;CHEBI:36357 denotes compounds
T752 23016-23022 SO:0000440 denotes vector
T751 23070-23080 CHEBI:39015;CHEBI:39015 denotes apoprotein
T750 23196-23204 SP_9 denotes MERS-CoV
T749 23337-23349 SO:0000409 denotes binding site
T748 23518-23526 SP_9 denotes MERS-CoV
T747 23718-23730 SO:0000409 denotes binding site
T746 23898-23906 SP_9 denotes MERS-CoV
T731 19419-19428 CHEBI:35222;CHEBI:35222 denotes inhibitor
T730 19437-19445 SP_9 denotes MERS-CoV
T729 19486-19494 SP_9 denotes MERS-CoV
T728 19635-19639 SP_9 denotes MERS
T727 19847-19855 SP_9 denotes MERS-CoV
T726 19856-19876 PG_4 denotes nucleocapsid protein
T725 19912-19932 PG_4 denotes Nucleocapsid protein
T724 19933-19944 GO:0010467 denotes expressions
T723 20002-20008 GO:0005634 denotes Nuclei
T722 20032-20036 CHEBI:51231;CHEBI:51231 denotes DAPI
T715 13865-13873 SP_9 denotes MERS-CoV
T714 13874-13883 PG_4 denotes N protein
T713 13924-13932 SP_9 denotes MERS-CoV
T712 13933-13942 PG_4 denotes N protein
T711 14098-14107 PG_4 denotes N protein
T710 14121-14128 GO:0032991 denotes complex
T709 14213-14222 PG_4 denotes N protein
T708 14240-14247 GO:0032991 denotes complex
T707 14340-14349 PG_4 denotes N protein
T706 14367-14374 GO:0032991 denotes complex
T705 14588-14596 SP_9 denotes MERS-CoV
T704 14597-14606 PG_4 denotes N protein
T703 14680-14684 CHEBI:9754;CHEBI:9754 denotes Tris
T702 14685-14688 CHEBI:17883;CHEBI:17883 denotes HCl
T701 14697-14701 CHEBI:26710;CHEBI:26710 denotes NaCl
T700 14862-14874 GO:0046983 denotes dimerization
T699 14906-14918 GO:0046983 denotes dimerization
T698 15021-15030 PG_4 denotes N protein
T697 15134-15141 SO:0000409 denotes surface
T696 15194-15211 GO:0005840 denotes ribonucleocapsids
T680 12495-12503 SP_9 denotes MERS-CoV
T679 12504-12513 PG_4 denotes N protein
T678 12705-12714 PG_4 denotes N protein
T677 12940-12944 CHEBI:9754;CHEBI:9754 denotes Tris
T676 12945-12948 CHEBI:17883;CHEBI:17883 denotes HCl
T675 12969-12973 CHEBI:26710;CHEBI:26710 denotes NaCl
T669 8607-8615 SP_9 denotes MERS-CoV
T668 8652-8660 SP_9 denotes MERS-CoV
T667 8791-8803 GO:0046983 denotes dimerization
T666 8871-8879 SP_9 denotes MERS-CoV
T665 8888-8900 GO:0051260 denotes Dimerization
T664 8923-8929 SO:0000440 denotes vector
T663 9456-9462 SO:0000440 denotes vector
T662 9737-9743 SO:0000440 denotes vector
T661 10011-10029 SO:0000366 denotes substitution sites
T660 10071-10083 GO:0046983 denotes dimerization
T652 37658-37662 CL_6 denotes Vero
T651 37761-37769 SP_9 denotes MERS-CoV
T650 37902-37914 CHEBI:9750;CHEBI:9750 denotes Triton X-100
T649 38004-38012 SP_9 denotes MERS-CoV
T648 38023-38031 GO:0042571 denotes antibody
T647 38109-38114 NCBITaxon:10239 denotes virus
T646 38195-38209 CHEBI:51766;CHEBI:51766 denotes Alex Fluor 568
T645 38215-38221 NCBITaxon:9986 denotes rabbit
T644 38232-38240 GO:0042571 denotes antibody
T643 38327-38344 CHEBI:51231;CHEBI:51231 denotes 4′,6-Diamidino-2-
T642 38344-38356 CHEBI:48559;CHEBI:48559 denotes phenylindole
T641 38358-38362 CHEBI:51231;CHEBI:51231 denotes DAPI
T640 38395-38399 SP_9 denotes MERS
T639 38413-38423 GO:0010467 denotes expression
T627 36913-36917 CL_6 denotes Vero
T626 37038-37059 GO:0001171 denotes Reverse transcription
T625 37288-37294 SO:0000112 denotes primer
T624 37321-37326 NCBITaxon:10239 denotes viral
T623 37354-37355 SO:0001030 denotes F
T622 37390-37391 SO:0001031 denotes R
T621 37423-37431 SP_9 denotes MERS-CoV
T620 37432-37433 SO:0001030 denotes F
T619 37464-37472 SP_9 denotes MERS-CoV
T618 37473-37474 SO:0001031 denotes R
T617 37516-37520 SP_9 denotes MERS
T616 37586-37594 SP_9 denotes MERS-CoV
T605 36339-36343 CL_6 denotes Vero
T604 36445-36453 SP_9 denotes MERS-CoV
T603 36676-36683 CHEBI:2511;CHEBI:2511 denotes agarose
T602 36752-36755 CHEBI:16526;CHEBI:16526 denotes CO2
T601 36805-36813 CHEBI:16842;CHEBI:16842 denotes formalin
T600 36836-36850 CHEBI:41688;CHEBI:41688 denotes crystal violet
T591 35876-35880 CL_6 denotes Vero
T590 36042-36050 SP_9 denotes MERS-CoV
T589 36258-36263 NCBITaxon:10239 denotes virus
T579 33998-34006 SP_9 denotes MERS-CoV
T578 34013-34021 SP_9 denotes MERS-CoV
T577 34027-34034 GO:0032991 denotes complex
T576 34145-34149 CHEBI:9754;CHEBI:9754 denotes Tris
T575 34150-34153 CHEBI:17883;CHEBI:17883 denotes HCl
T574 34174-34178 CHEBI:26710;CHEBI:26710 denotes NaCl
T573 34325-34333 CHEBI:75958;CHEBI:75958 denotes solution
T572 34510-34516 SO:0000440 denotes vector
T571 35264-35272 SP_9 denotes MERS-CoV
T570 35298-35306 SP_9 denotes MERS-CoV
T569 35349-35355 SO:0000417 denotes domain
T568 35359-35367 SP_9 denotes MERS-CoV
T567 35368-35377 PG_4 denotes N protein
T566 35699-35707 SP_9 denotes MERS-CoV
T565 35736-35744 SP_9 denotes MERS-CoV
T552 33347-33351 CL_6 denotes Vero
T551 33380-33388 SP_9 denotes MERS-CoV
T550 33428-33437 CHEBI:36357;CHEBI:36357 denotes compounds
T549 33485-33496 CHEBI:86370;CHEBI:86370 denotes neutral red
T548 33605-33610 CHEBI:23888;CHEBI:23888 denotes drugs
T547 33641-33645 SP_9 denotes MERS
T546 33667-33671 CHEBI:23888;CHEBI:23888 denotes drug
T541 32799-32814 GO:0031649 denotes Thermostability
T540 32898-32902 CHEBI:9754;CHEBI:9754 denotes Tris
T539 32903-32906 CHEBI:17883;CHEBI:17883 denotes HCl
T538 32927-32931 CHEBI:26710;CHEBI:26710 denotes NaCl
T537 33044-33053 PG_4 denotes N protein
T536 33107-33115 CHEBI:36357;CHEBI:36357 denotes compound
T533 32436-32440 CHEBI:9754;CHEBI:9754 denotes Tris
T532 32441-32444 CHEBI:17883;CHEBI:17883 denotes HCl
T531 32465-32469 CHEBI:26710;CHEBI:26710 denotes NaCl
T530 32486-32495 PG_4 denotes N protein
T529 32549-32557 CHEBI:36357;CHEBI:36357 denotes compound
T525 31703-31707 SP_9 denotes MERS
T524 31708-31717 PG_4 denotes N Protein
T523 31732-31741 CHEBI:36357;CHEBI:36357 denotes compounds
T522 31778-31782 SP_9 denotes MERS
T521 31810-31818 SP_9 denotes MERS-CoV
T520 31878-31882 CHEBI:23888;CHEBI:23888 denotes drug
T519 31938-31942 CHEBI:23888;CHEBI:23888 denotes drug
T518 32017-32026 CHEBI:36357;CHEBI:36357 denotes compounds
T517 32041-32050 PG_4 denotes N protein
T516 32056-32065 PG_4 denotes N protein
T515 32123-32131 CHEBI:36357;CHEBI:36357 denotes compound
T510 31283-31292 CHEBI:75958;CHEBI:75958 denotes solutions
T509 31334-31342 SP_9 denotes MERS-CoV
T508 31369-31383 CHEBI:64276;CHEBI:64276 denotes glutaraldehyde
T507 31516-31520 CHEBI:9754;CHEBI:9754 denotes Tris
T506 31521-31524 CHEBI:17883;CHEBI:17883 denotes HCl
T505 31587-31609 CHEBI:8984;CHEBI:8984 denotes sodium dodecyl sulfate
T504 31646-31649 CHEBI:8984;CHEBI:8984 denotes SDS
T496 29849-29857 SP_9 denotes MERS-CoV
T495 29878-29883 GO:0040007 denotes grown
T494 29911-29919 SP_9 denotes MERS-CoV
T493 30026-30034 CHEBI:75958;CHEBI:75958 denotes solution
T492 30104-30112 CHEBI:75958;CHEBI:75958 denotes solution
T491 30133-30149 CHEBI:62946;CHEBI:62946 denotes ammonium sulfate
T490 30156-30160 CHEBI:26710;CHEBI:26710 denotes NaBr
T489 30170-30173 CHEBI:53250;CHEBI:53250 denotes PEG
T488 30250-30258 CHEBI:75958;CHEBI:75958 denotes solution
T487 30260-30268 SP_9 denotes MERS-CoV
T486 30328-30336 CHEBI:75958;CHEBI:75958 denotes solution
T406 26104-26112 SP_9 denotes MERS-CoV
T405 26298-26311 NCBITaxon:11118 denotes coronaviruses
T404 26502-26511 CHEBI:36357;CHEBI:36357 denotes compounds
T403 26585-26593 SP_7 denotes COVID-19
T402 26616-26621 NCBITaxon:10088 denotes mouse
T401 26622-26631 NCBITaxon:5690 denotes hepatitis
T400 26632-26637 NCBITaxon:10239 denotes virus
T399 26639-26642 NCBITaxon:11757 denotes MHV
T398 26672-26677 NCBITaxon:10239 denotes viral
T397 26721-26724 NCBITaxon:11757 denotes MHV
T396 26725-26736 GO:0006260 denotes replication
T395 27527-27536 CHEBI:36357;CHEBI:36357 denotes compounds
T394 27670-27681 GO:0051259 denotes oligomerize
T393 27693-27701 SP_9 denotes MERS-CoV
T392 27702-27711 PG_4 denotes N protein
T391 27797-27805 CHEBI:36357;CHEBI:36357 denotes molecule
T390 28008-28012 CHEBI:23888;CHEBI:23888 denotes drug
T389 28078-28087 CHEBI:36357;CHEBI:36357 denotes compounds
T369 20060-20068 SP_9 denotes MERS-CoV
T368 20075-20084 GO:0032991 denotes Complexed
T367 20142-20150 SP_9 denotes MERS-CoV
T366 20160-20167 GO:1990524 denotes complex
T365 20173-20182 CHEBI:36357;CHEBI:36357 denotes compounds
T364 20268-20275 GO:0032991 denotes complex
T363 20413-20422 GO:0032991 denotes complexes
T362 20445-20453 SP_9 denotes MERS-CoV
T361 20460-20469 GO:0032991 denotes complexes
T360 20598-20607 CHEBI:36357;CHEBI:36357 denotes compounds
T359 20612-20620 SP_9 denotes MERS-CoV
T358 20668-20677 PG_4 denotes N protein
T357 20687-20695 CHEBI:36357;CHEBI:36357 denotes compound
T356 20869-20876 GO:0032991 denotes complex
T355 21101-21106 CHEBI:22563;CHEBI:22563 denotes anion
T354 21131-21143 CHEBI:52879;CHEBI:52879 denotes benzene ring
T353 21154-21162 CHEBI:43295;CHEBI:43295 denotes indoline
T352 21253-21260 CHEBI:52879;CHEBI:52879 denotes benzene
T351 21279-21289 CHEBI:50325;CHEBI:50325 denotes side chain
T350 21485-21499 SO:0000409 denotes binding region
T349 21794-21803 PG_4 denotes N protein
T348 21878-21890 SO:0000409 denotes binding site
T347 21938-21945 CHEBI:52879;CHEBI:52879 denotes benzene
T346 22128-22134 CHEBI:36916;CHEBI:36916 denotes cation
T345 22192-22198 CHEBI:24433;CHEBI:24433 denotes groups
T344 22548-22557 CHEBI:36357;CHEBI:36357 denotes compounds
T321 17867-17875 SP_9 denotes MERS-CoV
T320 17888-17897 PG_4 denotes N Protein
T319 18013-18021 SP_9 denotes MERS-CoV
T318 18082-18095 GO:0005576 denotes extracellular
T317 18096-18101 NCBITaxon:10239 denotes viral
T316 18113-18126 GO:0005622 denotes intracellular
T315 18127-18132 NCBITaxon:10239 denotes viral
T314 18178-18182 CL_6 denotes Vero
T313 18283-18288 NCBITaxon:10239 denotes viral
T312 18321-18326 NCBITaxon:10239;GO:0006260 denotes viral
T311 18327-18342 GO:0039703 denotes RNA replication
T310 18377-18382 NCBITaxon:10239;GO:0006260 denotes viral
T309 18383-18393 GO:0006260 denotes production
T308 18398-18409 GO:0006260 denotes replication
T307 18476-18484 CHEBI:36357;CHEBI:36357 denotes compound
T306 18503-18511 SP_9 denotes MERS-CoV
T305 18512-18521 PG_4 denotes N protein
T304 18539-18549 GO:0010467 denotes expression
T303 18700-18713 GO:0005622 denotes intracellular
T302 18714-18723 PG_4 denotes N protein
T301 18756-18760 CL_6 denotes Vero
T300 18813-18826 GO:0005622 denotes intracellular
T299 18827-18836 PG_4 denotes N protein
T298 18875-18876 PG_4 denotes N
T297 18877-18884 PG_4;BV_20 denotes protein
T296 18885-18895 BV_20;GO:0010467 denotes expression
T295 18949-18958 PG_4 denotes N protein
T294 18995-19003 SP_9 denotes MERS-CoV
T293 19171-19179 SP_9 denotes MERS-CoV
T292 19230-19238 SP_9 denotes MERS-CoV
T291 19279-19288 PG_4 denotes N protein
T272 13359-13367 SP_9 denotes MERS-CoV
T271 13368-13377 PG_4 denotes N Protein
T270 13450-13458 SP_9 denotes MERS-CoV
T269 13459-13468 PG_4 denotes N protein
T268 13741-13749 SP_9 denotes MERS-CoV
T267 13750-13759 PG_4 denotes N protein
T266 13763-13771 CHEBI:75958;CHEBI:75958 denotes solution
T265 15278-15287 PG_4 denotes N protein
T264 15433-15439 SO:0000417 denotes domain
T263 15487-15494 SO:0000417 denotes domains
T262 15574-15583 PG_4 denotes N protein
T261 15592-15599 GO:0032991 denotes complex
T260 15783-15792 PG_4 denotes N protein
T259 15854-15862 CHEBI:75958;CHEBI:75958 denotes solution
T258 15900-15908 CHEBI:75958;CHEBI:75958 denotes solution
T257 15997-16004 GO:0032991 denotes complex
T256 16295-16303 SP_9 denotes MERS-CoV
T255 16304-16313 PG_4 denotes N protein
T254 16381-16390 PG_4 denotes N protein
T253 16585-16597 GO:0046983 denotes dimerization
T252 16608-16616 SP_9 denotes MERS-CoV
T251 16632-16641 PG_4 denotes N protein
T250 16655-16660 NCBITaxon:10239 denotes viral
T249 16661-16667 SO:0001026 denotes genome
T248 16676-16679 GO:1990904 denotes RNP
T247 16680-16687 GO:0005662 denotes complex
T246 17058-17061 SO:0000694 denotes RNP
T245 17694-17703 PG_4 denotes N protein
T244 17704-17713 CHEBI:36357;CHEBI:36357 denotes molecules
T243 17796-17804 SP_9 denotes MERS-CoV
T242 17831-17840 PG_4 denotes N protein
T219 10373-10382 CHEBI:36357;CHEBI:36357 denotes compounds
T218 10406-10412 SO:0000440 denotes vector
T217 10991-10995 CHEBI:23888;CHEBI:23888 denotes drug
T216 11109-11118 CHEBI:36357;CHEBI:36357 denotes compounds
T215 11158-11164 CHEBI:22744;CHEBI:22744 denotes Benzyl
T214 11164-11168 CHEBI:75508;CHEBI:75508 denotes -2-(
T213 11168-11181 CHEBI:24712;CHEBI:24712 denotes hydroxymethyl
T212 11181-11204 CHEBI:75508;CHEBI:75508 denotes )-1-indolinecarboxylate
T211 11214-11215 CHEBI:75508;CHEBI:75508 denotes 5
T210 11216-11232 CHEBI:41237;CHEBI:41237 denotes benzyloxygramine
T209 11302-11306 CHEBI:23888;CHEBI:23888 denotes drug
T208 11307-11315 CHEBI:4909;CHEBI:4909 denotes etodolac
T207 11717-11726 PG_4 denotes N protein
T206 11864-11871 GO:0032508 denotes melting
T205 11948-11955 GO:0032508 denotes melting
T204 11966-11974 SP_9 denotes MERS-CoV
T203 12025-12045 GO:0030164 denotes protein denaturation
T202 12078-12086 SP_9 denotes MERS-CoV
T201 12218-12226 CHEBI:36357;CHEBI:36357 denotes compound
T200 12233-12237 CL_6 denotes Vero
T199 12261-12269 SP_9 denotes MERS-CoV
T198 12342-12351 CHEBI:36357;CHEBI:36357 denotes compounds
T197 12414-12423 CHEBI:35222;CHEBI:35222 denotes inhibitor
T196 12432-12440 SP_9 denotes MERS-CoV
T170 4790-4796 SO:0000417 denotes Domain
T169 4804-4812 SP_9 denotes MERS-CoV
T168 4813-4822 PG_4 denotes N Protein
T167 4862-4870 SP_9 denotes MERS-CoV
T166 5157-5166 CHEBI:36357;CHEBI:36357 denotes molecules
T165 5383-5396 SO:0000984 denotes five-stranded
T164 5811-5819 SP_9 denotes MERS-CoV
T163 5820-5829 PG_4 denotes N protein
T162 5940-5952 SO:0000409 denotes binding site
T161 6113-6122 PG_4 denotes N protein
T160 6242-6252 CHEBI:50325;CHEBI:50325 denotes side chain
T159 6470-6481 CHEBI:50325;CHEBI:50325 denotes side chains
T158 6732-6743 CHEBI:50325;CHEBI:50325 denotes side chains
T157 6828-6838 CHEBI:50325;CHEBI:50325 denotes side chain
T156 7299-7305 SO:0000440 denotes vector
T155 7508-7517 PG_4 denotes N protein
T154 7599-7603 SP_9 denotes MERS
T153 7625-7631 SO:0000440 denotes vector
T152 7651-7659 CHEBI:75958;CHEBI:75958 denotes solution
T151 7661-7665 SP_9 denotes MERS
T150 7702-7710 CHEBI:75958;CHEBI:75958 denotes solution
T149 7822-7828 SO:0000440 denotes vector
T148 8051-8057 SO:0000440 denotes vector
T147 8174-8182 SP_9 denotes MERS-CoV
T146 8283-8293 CHEBI:50325;CHEBI:50325 denotes side chain
T145 8467-8476 CHEBI:36357;CHEBI:36357 denotes compounds
T144 8492-8498 SO:0000440 denotes vector
T124 1341-1349 CHEBI:36357;CHEBI:36357 denotes molecule
T123 1441-1445 CHEBI:23888;CHEBI:23888 denotes drug
T122 1493-1498 NCBITaxon:10239 denotes viral
T121 1542-1551 CHEBI:36357;CHEBI:36357 denotes molecules
T120 1631-1646 GO:0051260 denotes oligomerization
T119 1692-1701 CHEBI:36357;CHEBI:36357 denotes molecules
T118 1705-1713 GO:0065007 denotes modulate
T117 1749-1759 CHEBI:35718;CHEBI:35718 denotes anticancer
T116 1760-1764 CHEBI:23888;CHEBI:23888 denotes drug
T115 1765-1775 CHEBI:45863;CHEBI:45863 denotes paclitaxel
T114 1814-1825 GO:0005874;GO:0090307 denotes microtubule
T113 1826-1844 GO:0090307 denotes structure assembly
T112 1861-1868 PR:000028799 denotes tubulin
T111 1892-1901 CHEBI:33893;CHEBI:33893 denotes rapamycin
T110 1911-1927 CHEBI:67079;CHEBI:67079 denotes anticancer agent
T109 1969-1975 PR:000007536 denotes FKBP12
T108 2021-2028 GO:0032991 denotes complex
T107 2089-2093 CHEBI:23888;CHEBI:23888;GO:0017144 denotes drug
T106 2094-2105 GO:0017144 denotes development
T105 2282-2286 CHEBI:23888;CHEBI:23888 denotes drug
T104 2421-2434 PG_4 denotes nucleoprotein
T103 2454-2463 NCBITaxon:7719 denotes influenza
T102 2464-2469 NCBITaxon:10239 denotes virus
T101 2497-2520 GO:0051259 denotes protein oligomerization
T100 2533-2538 NCBITaxon:10239 denotes viral
T99 2552-2563 SP_9 denotes Middle East
T98 2564-2575 SP_9;UBERON:0001004 denotes respiratory
T97 2576-2596 SP_9 denotes syndrome coronavirus
T96 2598-2606 SP_9 denotes MERS-CoV
T95 2623-2638 SP_8;NCBITaxon:694002 denotes betacoronavirus
T94 2673-2684 UBERON:0001004 denotes respiratory
T93 2724-2730 NCBITaxon:9606 denotes humans
T92 2771-2782 NCBITaxon:11118 denotes coronavirus
T91 2784-2795 NCBITaxon:11118 denotes coronavirus
T90 2810-2818 SP_7 denotes COVID-19
T89 3072-3080 SP_9 denotes MERS-CoV
T88 3094-3100 SO:0001026 denotes genome
T87 3143-3160 GO:0005840 denotes ribonucleoprotein
T86 3167-3174 GO:0032991 denotes complex
T85 3201-3206 NCBITaxon:10239 denotes viral
T84 3270-3280 GO:0065007 denotes modulation
T83 3288-3289 PG_4 denotes N
T82 3290-3297 PG_4;GO:0051259 denotes protein
T81 3298-3313 GO:0051259 denotes oligomerization
T80 3323-3332 CHEBI:36357;CHEBI:36357 denotes molecules
T79 3357-3361 CHEBI:36044;CHEBI:36044 denotes drug
T78 3393-3396 NCBITaxon:11886 denotes CoV
T77 3397-3406 PG_4 denotes N protein
T76 3440-3446 SO:0000417 denotes domain
T75 3472-3478 SO:0000417 denotes domain
T74 3496-3503 SO:0000417 denotes domains
T73 3675-3676 GO:0051260;PG_4 denotes N
T72 3677-3684 PG_4;GO:0051259 denotes protein
T71 3685-3700 GO:0051259 denotes oligomerization
T485 30357-30365 SP_9 denotes MERS-CoV
T484 30384-30391 GO:1990524 denotes complex
T483 30432-30440 SP_9 denotes MERS-CoV
T482 30506-30514 CHEBI:75958;CHEBI:75958 denotes solution
T481 30560-30568 SP_9 denotes MERS-CoV
T480 30588-30595 GO:1990524 denotes complex
T479 30763-30771 SP_9 denotes MERS-CoV
T478 30781-30788 GO:0032991 denotes complex
T461 28473-28480 BV_20 denotes Protein
T460 28481-28491 BV_20;GO:0010467 denotes Expression
T459 28514-28522 SP_9 denotes MERS-CoV
T458 28625-28633 SP_9 denotes MERS-CoV
T457 28672-28682 GO:0010467 denotes expression
T456 28683-28689 SO:0000440 denotes vector
T455 28901-28908 SO:0000112 denotes primers
T454 28933-28940 SO:0000440 denotes vectors
T453 28946-28957 GO:0009294 denotes transformed
T452 28963-28979 NCBITaxon:562 denotes Escherichia coli
T451 29019-29024 GO:0040007 denotes grown
T450 29087-29094 BV_20 denotes protein
T449 29095-29105 BV_20;GO:0010467 denotes expression
T448 29126-29135 CHEBI:30353;CHEBI:30353 denotes isopropyl
T447 29136-29163 CHEBI:75055;CHEBI:75055 denotes β-d-1-thiogalactopyranoside
T446 29165-29169 CHEBI:61448;CHEBI:61448 denotes IPTG
T445 29298-29303 GO:0019835 denotes lysis
T444 29319-29323 CHEBI:26710;CHEBI:26710 denotes NaCl
T443 29331-29335 CHEBI:9754;CHEBI:9754 denotes Tris
T442 29336-29339 CHEBI:17883;CHEBI:17883 denotes HCl
T441 29347-29356 CHEBI:14434;CHEBI:14434 denotes imidazole
T440 29367-29371 CHEBI:8102;CHEBI:8102 denotes PMSF
T439 29397-29402 GO:0019835 denotes lysed
T438 29526-29529 CHEBI:39054;CHEBI:39054 denotes NTA
T437 29599-29608 CHEBI:14434;CHEBI:14434 denotes imidazole
T436 29700-29704 CHEBI:24866;CHEBI:24866 denotes salt
T427 28152-28161 CHEBI:36357;CHEBI:36357 denotes compounds
T426 28289-28297 CHEBI:33893;CHEBI:33893 denotes reagents
T425 28391-28400 CHEBI:36357;CHEBI:36357 denotes compounds
T424 24092-24101 CHEBI:36357;CHEBI:36357 denotes molecules
T423 24215-24224 CHEBI:36357;CHEBI:36357 denotes compounds
T422 24378-24387 CHEBI:36357;CHEBI:36357 denotes compounds
T421 24630-24643 PG_4 denotes nucleoprotein
T420 24719-24728 CHEBI:25106;CHEBI:25106 denotes macrolide
T419 24760-24778 GO:0015629 denotes actin cytoskeleton
T418 24793-24803 CHEBI:67079;CHEBI:67079 denotes anticancer
T417 24864-24865 GO:0000811 denotes G
T416 24865-24871 GO:0031941 denotes -actin
T415 25344-25362 CHEBI:36357;CHEBI:36357 denotes molecule compounds
T414 25610-25618 SP_9 denotes MERS-CoV
T413 25661-25669 SP_9 denotes MERS-CoV
T412 25676-25688 GO:0046983 denotes dimerization
T411 25706-25715 PG_4 denotes N protein
T410 25815-25818 SO:0000694 denotes RNP
T409 26045-26049 CHEBI:23888;CHEBI:23888;GO:0017144 denotes drug
T408 26050-26061 GO:0017144 denotes development
T407 26082-26095 NCBITaxon:11118 denotes coronaviruses
T70 3781-3789 SP_9 denotes MERS-CoV
T69 4108-4112 CHEBI:23888;CHEBI:23888 denotes drug
T68 4141-4157 CHEBI:41237;CHEBI:41237 denotes benzyloxygramine
T67 4216-4225 PG_4 denotes N protein
T66 4329-4330 PG_4 denotes N
T65 4331-4338 PG_4;GO:0051259 denotes protein
T64 4339-4354 GO:0051259 denotes oligomerization
T63 4422-4430 SP_9 denotes MERS-CoV
T62 4437-4446 GO:0032991 denotes complexed
T61 4452-4470 CHEBI:41237;CHEBI:41237 denotes 5-benzyloxygramine
T21 242-246 CHEBI:23888;CHEBI:23888 denotes drug
T20 509-515 SO:0000417 denotes domain
T19 523-531 SP_9 denotes MERS-CoV
T18 532-552 PG_4 denotes nucleocapsid protein
T17 554-562 SP_9 denotes MERS-CoV
T16 645-649 CHEBI:23888;CHEBI:23888 denotes drug
T15 757-775 CHEBI:41237;CHEBI:41237 denotes 5-benzyloxygramine
T14 785-794 PG_4 denotes N protein
T13 960-978 CHEBI:41237;CHEBI:41237 denotes 5-benzyloxygramine
T12 1095-1096 PG_4 denotes N
T11 1097-1104 PG_4;GO:0051259 denotes protein
T10 1105-1120 GO:0051259 denotes oligomerization
T9 1250-1259 PG_4 denotes N protein
T8 1284-1288 CHEBI:23888;CHEBI:23888 denotes drug
T22 123-127 CHEBI:36044;CHEBI:36044 denotes Drug
T23 88-100 GO:0000786 denotes Nucleocapsid
T24 76-87 NCBITaxon:11118 denotes Coronavirus

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T1 44-51 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T2 52-59 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T3 101-109 Body_part denotes Proteins http://purl.org/sig/ont/fma/fma67257
T4 178-185 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T5 186-193 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T6 545-552 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T7 787-794 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T8 861-868 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T9 1097-1104 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T10 1155-1159 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T11 1252-1259 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T12 1367-1374 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T13 1375-1382 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T14 1666-1673 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T15 1814-1825 Body_part denotes microtubule http://purl.org/sig/ont/fma/fma63849
T16 1861-1868 Body_part denotes tubulin http://purl.org/sig/ont/fma/fma67116
T17 1886-1890 Body_part denotes hand http://purl.org/sig/ont/fma/fma9712
T18 2497-2504 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T19 3094-3100 Body_part denotes genome http://purl.org/sig/ont/fma/fma84116
T20 3123-3130 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T21 3290-3297 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T22 3399-3406 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T23 3521-3524 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T24 3677-3684 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T25 3705-3712 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T26 3713-3720 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T27 4218-4225 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T28 4267-4271 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T29 4331-4338 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T30 4375-4379 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T31 4625-4632 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T32 4815-4822 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T33 5457-5469 Body_part denotes right-handed http://purl.org/sig/ont/fma/fma9713
T34 5630-5638 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T35 5822-5829 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T36 5910-5920 Body_part denotes amino acid http://purl.org/sig/ont/fma/fma82739
T37 6115-6122 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T38 6540-6548 Body_part denotes backbone http://purl.org/sig/ont/fma/fma13478
T39 7053-7055 Body_part denotes Å2 http://purl.org/sig/ont/fma/fma66595
T40 7510-7517 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T41 8058-8066 Body_part denotes backbone http://purl.org/sig/ont/fma/fma13478
T42 9549-9556 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T43 9898-9906 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T44 11008-11013 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T45 11564-11575 Body_part denotes tryptophans http://purl.org/sig/ont/fma/fma82767
T46 11583-11590 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T47 11719-11726 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T48 12025-12032 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T49 12241-12246 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T50 12506-12513 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T51 12707-12714 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T52 12762-12770 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T53 13306-13311 Body_part denotes d NA http://purl.org/sig/ont/fma/fma74412
T54 13370-13377 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T55 13461-13468 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T56 13529-13536 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T57 13627-13633 Body_part denotes radius http://purl.org/sig/ont/fma/fma23463
T58 13658-13665 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T59 13752-13759 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T60 13876-13883 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T61 13935-13942 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T62 14047-14053 Body_part denotes radius http://purl.org/sig/ont/fma/fma23463
T63 14100-14107 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T64 14215-14222 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T65 14342-14349 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T66 14599-14606 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T67 14788-14791 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T68 14795-14803 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T69 14995-15002 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T70 15023-15030 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T71 15122-15125 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T72 15280-15287 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T73 15382-15386 Body_part denotes body http://purl.org/sig/ont/fma/fma256135
T74 15576-15583 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T75 15706-15713 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T76 15785-15792 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T77 15976-15984 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T78 16306-16313 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T79 16383-16390 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T80 16491-16498 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T81 16634-16641 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T82 16661-16667 Body_part denotes genome http://purl.org/sig/ont/fma/fma84116
T83 16879-16885 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T84 16916-16919 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T85 17016-17022 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T86 17107-17110 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T87 17233-17239 Body_part denotes cuboid http://purl.org/sig/ont/fma/fma24527
T88 17262-17265 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T89 17449-17456 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T90 17572-17579 Body_part denotes cuboids http://purl.org/sig/ont/fma/fma24527
T91 17633-17636 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T92 17696-17703 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T93 17833-17840 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T94 17890-17897 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T95 18044-18048 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T96 18133-18136 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T97 18186-18191 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T98 18327-18330 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T99 18514-18521 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T100 18566-18571 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T101 18716-18723 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T102 18764-18769 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T103 18829-18836 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T104 18877-18884 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T105 18904-18909 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T106 18951-18958 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T107 19006-19014 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T108 19035-19040 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T109 19138-19143 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T110 19281-19288 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T111 19300-19304 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T112 19475-19478 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T113 19595-19598 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T114 19672-19675 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T115 19869-19876 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T116 19925-19932 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T117 20670-20677 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T118 21454-21464 Body_part denotes amino acid http://purl.org/sig/ont/fma/fma82739
T119 21700-21708 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T120 21796-21803 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T121 24760-24778 Body_part denotes actin cytoskeleton http://purl.org/sig/ont/fma/fma84684
T122 24864-24871 Body_part denotes G-actin http://purl.org/sig/ont/fma/fma67893
T123 25016-25023 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T124 25024-25031 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T125 25036-25043 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T126 25554-25561 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T127 25708-25715 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T128 26017-26025 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T129 26118-26129 Body_part denotes amino acids http://purl.org/sig/ont/fma/fma82739
T130 26763-26767 Body_part denotes hand http://purl.org/sig/ont/fma/fma9712
T131 27152-27159 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T132 27650-27658 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T133 27704-27711 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T134 28473-28480 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T135 28525-28533 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T136 28636-28644 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T137 28731-28740 Body_part denotes histidine http://purl.org/sig/ont/fma/fma82755
T138 28997-29002 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T139 29008-29013 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T140 29087-29094 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T141 29218-29223 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T142 29386-29391 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T143 29648-29655 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T144 30018-30025 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T145 31275-31282 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T146 31710-31717 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T147 32043-32050 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T148 32058-32065 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T149 32488-32495 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T150 32583-32593 Body_part denotes Tryptophan http://purl.org/sig/ont/fma/fma82767
T151 33046-33053 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T152 33355-33360 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T153 33448-33452 Body_part denotes Cell http://purl.org/sig/ont/fma/fma68646
T154 33546-33550 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T155 33586-33591 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T156 33655-33660 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T157 33975-33982 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T158 34075-34082 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T159 34361-34370 Body_part denotes capillary http://purl.org/sig/ont/fma/fma63194
T160 35139-35143 Body_part denotes body http://purl.org/sig/ont/fma/fma256135
T161 35370-35377 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T162 35884-35889 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T163 36131-36136 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T164 36347-36352 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T165 36897-36900 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T166 36921-36926 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T167 37327-37330 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T168 37521-37524 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T169 37666-37671 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T170 37791-37796 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T171 38023-38031 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T172 38120-38125 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T173 38232-38240 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T174 38486-38488 Body_part denotes AG http://purl.org/sig/ont/fma/fma61898
T175 38825-38832 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T176 38848-38851 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T177 40496-40503 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T178 40504-40511 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T179 40892-40898 Body_part denotes radius http://purl.org/sig/ont/fma/fma23463

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1886-1890 Body_part denotes hand http://purl.obolibrary.org/obo/UBERON_0002398
T2 26763-26767 Body_part denotes hand http://purl.obolibrary.org/obo/UBERON_0002398
T3 34361-34370 Body_part denotes capillary http://purl.obolibrary.org/obo/UBERON_0001982

LitCovid_AGAC

Id Subject Object Predicate Lexical cue
p35652s4 1631-1658 MPA denotes oligomerization equilibrium
p35652s8 1666-1673 Protein denotes protein
p35652s29 1805-1813 PosReg denotes enhances
p35652s30 1814-1844 MPA denotes microtubule structure assembly
p35655s32 2505-2520 MPA denotes oligomerization
p35655s34 2525-2529 NegReg denotes loss
p35655s37 2539-2548 MPA denotes viability
p35702s4 7929-7936 NegReg denotes reduced
p35702s6 7941-7960 MPA denotes oligomeric tendency
p35702s9 7964-7969 MPA denotes N-NTD
p35813s5 16373-16380 NegReg denotes delayed
p35813s6 16381-16411 MPA denotes N protein thermal denaturation
p35853s3 19847-19851 Protein denotes MERS
p35853s7 19869-19876 MPA denotes protein
p35853s8 19877-19886 NegReg denotes decreased
p35869s25 21774-21786 MPA denotes conformation
p35904s12 24748-24755 NegReg denotes disrupt
p35904s14 24760-24778 MPA denotes actin cytoskeleton
p35911s11 25697-25727 MPA denotes abnormal N protein aggregation
p35918s15 26659-26668 NegReg denotes reduction

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 565-568 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T3 857-860 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T5 996-999 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T7 1493-1509 Disease denotes viral infections http://purl.obolibrary.org/obo/MONDO_0005108
T8 2454-2463 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T9 2784-2808 Disease denotes coronavirus disease 2019 http://purl.obolibrary.org/obo/MONDO_0100096
T10 2810-2818 Disease denotes COVID-19 http://purl.obolibrary.org/obo/MONDO_0100096
T11 2843-2852 Disease denotes pneumonia http://purl.obolibrary.org/obo/MONDO_0005249
T12 3448-3451 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T14 3549-3552 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T16 3792-3795 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T18 4433-4436 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T20 4731-4741 Disease denotes infectious http://purl.obolibrary.org/obo/MONDO_0005550
T21 4873-4876 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T23 4942-4945 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T25 5153-5156 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T27 7606-7609 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T29 7668-7671 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T31 7876-7879 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T33 7966-7969 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T35 8185-8188 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T37 8618-8621 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T39 8663-8666 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T41 10295-10298 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T43 10667-10670 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T45 10888-10891 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T47 11484-11487 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T49 11847-11850 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T51 11977-11980 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T53 12089-12092 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T55 12858-12861 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T57 14455-14458 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T59 15441-15444 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T61 15832-15835 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T63 16123-16126 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T65 16581-16584 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T67 17362-17365 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T69 19737-19739 Disease denotes SE http://purl.obolibrary.org/obo/MONDO_0002125
T70 20071-20074 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T72 20153-20156 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T74 20292-20295 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T76 20623-20626 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T78 21436-21439 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T80 22427-22430 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T82 22594-22597 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T84 22676-22679 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T86 23207-23210 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T88 23529-23532 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T90 23909-23912 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T92 24500-24509 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T93 25672-25675 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T95 26183-26186 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T97 26585-26593 Disease denotes COVID-19 http://purl.obolibrary.org/obo/MONDO_0100096
T98 26622-26631 Disease denotes hepatitis http://purl.obolibrary.org/obo/MONDO_0002251
T99 29860-29863 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T101 29922-29925 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T103 30368-30371 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T105 30443-30446 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T107 30571-30574 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T109 31049-31052 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T111 31345-31348 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T113 31821-31824 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T115 33821-33834 Disease denotes monochromatic http://purl.obolibrary.org/obo/MONDO_0018852
T116 35273-35276 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T118 35860-35875 Disease denotes Viral Infection http://purl.obolibrary.org/obo/MONDO_0005108
T119 35866-35875 Disease denotes Infection http://purl.obolibrary.org/obo/MONDO_0005550
T120 36031-36040 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T121 36057-36060 Disease denotes EMC http://purl.obolibrary.org/obo/MONDO_0012825
T122 36088-36097 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T123 37746-37755 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T124 38693-38696 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T126 38745-38748 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T128 39029-39038 Disease denotes hepatitis http://purl.obolibrary.org/obo/MONDO_0002251
T129 40579-40582 Disease denotes NTD http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075
T131 40921-40930 Disease denotes hepatitis http://purl.obolibrary.org/obo/MONDO_0002251

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T1 217-218 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T2 281-284 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T3 292-299 http://purl.obolibrary.org/obo/CLO_0009985 denotes focused
T4 413-414 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T5 592-593 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T6 616-622 http://purl.obolibrary.org/obo/UBERON_0002553 denotes cavity
T7 779-780 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T8 881-891 http://purl.obolibrary.org/obo/CLO_0001658 denotes activities
T9 1155-1159 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T10 1409-1418 http://www.ebi.ac.uk/efo/EFO_0000876 denotes extremely
T11 2207-2214 http://www.ebi.ac.uk/efo/EFO_0000876 denotes extreme
T12 2244-2245 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T13 2340-2348 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T14 2464-2469 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T15 2549-2551 http://purl.obolibrary.org/obo/CLO_0053733 denotes 11
T16 2699-2700 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T17 2724-2730 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes humans
T18 2747-2748 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T19 3104-3105 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T20 3141-3142 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T21 3336-3337 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T22 3410-3419 http://purl.obolibrary.org/obo/OBI_0000245 denotes organized
T23 3578-3579 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T24 3799-3800 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T25 4082-4084 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T26 4198-4208 http://purl.obolibrary.org/obo/CLO_0001658 denotes activities
T27 4267-4271 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T28 4375-4379 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T29 4564-4565 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T30 4612-4613 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T31 4903-4905 http://purl.obolibrary.org/obo/CLO_0007860 denotes MR
T32 5081-5082 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T33 5101-5102 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T34 5110-5112 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T35 5232-5233 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T36 5265-5266 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T37 5289-5290 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T38 5327-5328 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T39 5381-5382 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T40 5455-5456 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T41 5692-5693 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T42 6196-6197 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T43 6295-6296 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T44 6560-6564 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T45 6565-6566 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T46 6583-6584 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T47 6771-6775 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T48 6807-6808 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T49 6867-6868 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T50 6929-6933 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1 at
T51 6934-6935 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T52 6952-6953 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T53 7053-7055 http://purl.obolibrary.org/obo/CLO_0001562 denotes Å2
T54 7053-7055 http://purl.obolibrary.org/obo/CLO_0001563 denotes Å2
T55 7183-7184 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T56 7425-7428 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T57 7676-7677 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T58 8203-8205 http://purl.obolibrary.org/obo/CLO_0050509 denotes 27
T59 8217-8218 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T60 8624-8625 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T61 8716-8717 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T62 8844-8850 http://purl.obolibrary.org/obo/CLO_0001869 denotes B). (B
T63 9131-9138 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T64 9345-9346 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T65 9776-9783 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T66 10207-10208 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T67 10491-10493 http://purl.obolibrary.org/obo/CLO_0001236 denotes 2A
T68 10502-10503 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T69 10557-10559 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T70 10557-10559 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T71 11008-11013 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T72 11206-11208 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T73 11317-11319 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T74 11325-11326 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T75 11347-11348 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T76 11393-11394 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T77 11431-11432 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T78 11732-11734 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T79 11738-11740 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T80 11909-11911 http://purl.obolibrary.org/obo/CLO_0053799 denotes 45
T81 12233-12246 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T82 12297-12298 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T83 12352-12358 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T84 12468-12469 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T85 12516-12517 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T86 12773-12774 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T87 12844-12846 http://purl.obolibrary.org/obo/CLO_0003358 denotes FL
T88 12884-12886 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T89 12911-12912 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T90 13120-13122 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T91 13142-13144 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T92 13187-13188 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T93 13233-13234 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T94 13682-13683 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T95 13702-13703 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T96 13886-13889 http://purl.obolibrary.org/obo/CLO_0051456 denotes A–E
T97 13945-13946 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T98 14088-14089 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T99 14180-14181 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T100 14224-14225 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T101 14570-14572 http://purl.obolibrary.org/obo/CLO_0003358 denotes FL
T102 14651-14652 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T103 14804-14812 http://purl.obolibrary.org/obo/OBI_0000245 denotes organize
T104 14804-14812 http://purl.obolibrary.org/obo/OBI_0100026 denotes organize
T105 14804-14812 http://purl.obolibrary.org/obo/UBERON_0000468 denotes organize
T106 14816-14817 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T107 15576-15599 http://purl.obolibrary.org/obo/GO_0043234 denotes protein and its complex
T108 15800-15801 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T109 16012-16013 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T110 16039-16040 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T111 16093-16094 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T112 16232-16233 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T113 16862-16867 http://purl.obolibrary.org/obo/UBERON_0001456 denotes faces
T114 16993-16998 http://purl.obolibrary.org/obo/UBERON_0001456 denotes faces
T115 17491-17492 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T116 17588-17589 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T117 17966-17967 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T118 18022-18030 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T119 18044-18048 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T120 18178-18191 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T121 18253-18258 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T122 18301-18303 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T123 18359-18364 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T124 18416-18418 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T125 18566-18571 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T126 18669-18671 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4C
T127 18737-18743 http://purl.obolibrary.org/obo/SO_0000418 denotes signal
T128 18756-18769 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T129 18787-18791 http://purl.obolibrary.org/obo/CLO_0007842 denotes M P3
T130 18858-18863 http://purl.obolibrary.org/obo/CLO_0007842 denotes M, P3
T131 18904-18909 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T132 18920-18921 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T133 18959-18966 http://purl.obolibrary.org/obo/SO_0000418 denotes signals
T134 19035-19040 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T135 19138-19143 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T136 19300-19304 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T137 19407-19408 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T138 19448-19449 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T139 19451-19452 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T140 19468-19469 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T141 19480-19481 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T142 19580-19582 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T143 19893-19895 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T144 19971-19972 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T145 20183-20185 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T146 20187-20189 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T147 20260-20262 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T148 20301-20303 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T149 20355-20356 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T150 20378-20380 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T151 20866-20868 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T152 20969-20971 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T153 20982-20983 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T154 21066-21068 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T155 21097-21098 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T156 21151-21153 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T157 21208-21209 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T158 21250-21252 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T159 21328-21330 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T160 21398-21399 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T161 21651-21652 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T162 22077-22078 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T163 22253-22254 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T164 22292-22293 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T165 22520-22530 http://purl.obolibrary.org/obo/CLO_0001658 denotes activities
T166 23108-23109 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T167 23116-23117 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T168 23215-23217 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T169 23219-23220 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T170 23222-23223 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T171 23250-23252 http://purl.obolibrary.org/obo/CLO_0003377 denotes Fo
T172 23253-23255 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T173 23288-23289 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T174 23334-23336 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T175 23437-23439 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T176 23459-23466 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T177 23490-23491 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T178 23506-23508 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T179 23563-23565 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T180 23840-23847 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T181 24130-24133 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T182 24151-24152 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T183 24347-24349 http://purl.obolibrary.org/obo/CLO_0001000 denotes 35
T184 24560-24561 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T185 24697-24701 http://purl.obolibrary.org/obo/CLO_0001627 denotes A, a
T186 24730-24733 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T187 24823-24826 http://purl.obolibrary.org/obo/CLO_0050884 denotes ten
T188 24875-24876 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T189 24906-24908 http://purl.obolibrary.org/obo/CLO_0053794 denotes 41
T190 24987-24988 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T191 25244-25252 http://purl.obolibrary.org/obo/CLO_0009985 denotes focusing
T192 25415-25416 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T193 25487-25488 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T194 25531-25539 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T195 25926-25929 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T196 26039-26040 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T197 26532-26540 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T198 26549-26550 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T199 26603-26609 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T200 26616-26621 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T201 26632-26637 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T202 26657-26658 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T203 26745-26747 http://purl.obolibrary.org/obo/CLO_0008933 denotes S5
T204 27367-27369 http://purl.obolibrary.org/obo/CLO_0007874 denotes MS
T205 27370-27372 http://purl.obolibrary.org/obo/CLO_0053799 denotes 45
T206 27986-27992 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T207 28162-28164 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T208 28166-28168 http://purl.obolibrary.org/obo/CLO_0008307 denotes P2
T209 28357-28359 http://purl.obolibrary.org/obo/CLO_0009141 denotes St
T210 28357-28359 http://purl.obolibrary.org/obo/CLO_0050980 denotes St
T211 28368-28370 http://purl.obolibrary.org/obo/CLO_0007815 denotes MO
T212 28662-28663 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T213 28729-28730 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T214 28997-29002 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T215 29008-29013 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T216 29218-29223 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T217 29273-29277 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T218 29386-29391 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T219 29450-29454 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T220 29521-29522 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T221 29612-29613 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T222 30016-30017 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T223 30048-30052 http://purl.obolibrary.org/obo/CLO_0007735 denotes mL–1
T224 30201-30203 http://purl.obolibrary.org/obo/CLO_0009141 denotes St
T225 30201-30203 http://purl.obolibrary.org/obo/CLO_0050980 denotes St
T226 30212-30214 http://purl.obolibrary.org/obo/CLO_0007815 denotes MO
T227 30241-30242 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T228 30310-30311 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T229 30397-30399 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T230 30488-30489 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T231 30531-30533 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T232 30601-30603 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T233 31015-31017 http://purl.obolibrary.org/obo/CLO_0007860 denotes MR
T234 31387-31388 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T235 31791-31792 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T236 31919-31927 http://purl.obolibrary.org/obo/OBI_0100026 denotes Organics
T237 31919-31927 http://purl.obolibrary.org/obo/UBERON_0000468 denotes Organics
T238 32100-32101 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T239 32162-32163 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T240 32338-32340 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T241 32338-32340 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T242 32407-32408 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T243 32569-32573 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T244 32625-32626 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T245 32869-32870 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T246 32941-32942 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T247 33127-33131 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T248 33236-33237 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T249 33347-33360 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T250 33442-33444 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T251 33448-33452 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T252 33546-33550 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T253 33586-33591 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T254 33655-33660 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T255 33819-33820 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T256 33857-33858 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T257 33920-33921 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T258 34093-34097 http://purl.obolibrary.org/obo/CLO_0007842 denotes M P3
T259 34116-34117 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T260 34201-34202 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T261 34250-34251 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T262 34352-34353 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T263 34497-34498 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T264 34538-34539 http://purl.obolibrary.org/obo/CLO_0001020 denotes Å
T265 35291-35293 http://purl.obolibrary.org/obo/CLO_0050509 denotes 27
T266 35508-35509 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T267 35708-35710 http://purl.obolibrary.org/obo/CLO_0008192 denotes NP
T268 35876-35889 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T269 36070-36071 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T270 36131-36136 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T271 36258-36263 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T272 36339-36352 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T273 36671-36674 http://purl.obolibrary.org/obo/CLO_0050644 denotes w/v
T274 36685-36688 http://purl.obolibrary.org/obo/CLO_0053733 denotes 1:1
T275 36831-36834 http://purl.obolibrary.org/obo/CLO_0050644 denotes w/v
T276 36913-36926 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T277 37210-37211 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T278 37354-37358 http://purl.obolibrary.org/obo/CLO_0002983 denotes F: 5
T279 37432-37436 http://purl.obolibrary.org/obo/CLO_0002983 denotes F: 5
T280 37622-37624 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T281 37658-37671 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T282 37791-37796 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T283 37853-37857 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T284 37960-37961 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T285 38109-38114 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T286 38120-38125 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T287 38443-38444 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T288 38705-38707 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T289 38779-38781 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T290 38779-38781 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T291 38995-39003 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T292 39023-39028 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T293 39039-39044 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T294 39054-39056 http://purl.obolibrary.org/obo/CLO_0008933 denotes S5
T295 39125-39127 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T296 39245-39247 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T297 39245-39247 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T298 39502-39504 http://purl.obolibrary.org/obo/CLO_0008285 denotes P1
T299 40717-40719 http://purl.obolibrary.org/obo/CLO_0007860 denotes MR
T300 40915-40920 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T301 40931-40936 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T1 44-51 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T2 52-59 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T3 178-185 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T4 186-193 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T5 242-246 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T6 545-552 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T7 645-649 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T8 787-794 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T9 795-798 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T11 811-821 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T12 841-850 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T13 861-868 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T14 1097-1104 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T15 1252-1259 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T16 1284-1288 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T17 1341-1349 Chemical denotes molecule http://purl.obolibrary.org/obo/CHEBI_25367
T18 1367-1374 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T19 1375-1382 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T20 1441-1445 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T21 1542-1551 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T22 1666-1673 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T23 1692-1701 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T24 1760-1764 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T25 1765-1775 Chemical denotes paclitaxel http://purl.obolibrary.org/obo/CHEBI_45863
T26 1892-1901 Chemical denotes rapamycin http://purl.obolibrary.org/obo/CHEBI_9168
T27 1911-1927 Chemical denotes anticancer agent http://purl.obolibrary.org/obo/CHEBI_35610
T28 2089-2093 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T29 2282-2286 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T30 2330-2339 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T31 2379-2382 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T33 2497-2504 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T34 3036-3052 Chemical denotes antiviral agents http://purl.obolibrary.org/obo/CHEBI_22587
T35 3123-3130 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T36 3290-3297 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T37 3323-3332 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T38 3347-3361 Chemical denotes antiviral drug http://purl.obolibrary.org/obo/CHEBI_36044
T39 3347-3356 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T40 3357-3361 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T41 3399-3406 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T42 3677-3684 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T43 3705-3712 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T44 3713-3720 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T45 3928-3938 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T46 4082-4084 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T47 4108-4112 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T48 4172-4181 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T49 4218-4225 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T50 4331-4338 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T51 4625-4632 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T52 4815-4822 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T53 4903-4905 Chemical denotes MR http://purl.obolibrary.org/obo/CHEBI_74698
T54 4951-4953 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T55 5157-5166 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T56 5630-5638 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T57 5822-5829 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T58 5910-5920 Chemical denotes amino acid http://purl.obolibrary.org/obo/CHEBI_33709
T59 5910-5915 Chemical denotes amino http://purl.obolibrary.org/obo/CHEBI_46882
T60 5916-5920 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527
T61 6115-6122 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T62 6332-6335 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T63 6513-6521 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T64 6708-6716 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T65 6869-6877 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T66 6903-6909 Chemical denotes oxygen http://purl.obolibrary.org/obo/CHEBI_25805
T67 7323-7326 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T68 7510-7517 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T69 7651-7659 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T70 7702-7710 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T71 8194-8196 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T72 8533-8536 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T74 9549-9556 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T75 9898-9906 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T76 10318-10321 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T77 10557-10559 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T78 10874-10881 Chemical denotes ligands http://purl.obolibrary.org/obo/CHEBI_52214
T79 10937-10939 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T80 10991-10995 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T81 11168-11181 Chemical denotes hydroxymethyl http://purl.obolibrary.org/obo/CHEBI_24712
T82 11206-11208 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T83 11249-11251 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T84 11302-11306 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T85 11307-11315 Chemical denotes etodolac http://purl.obolibrary.org/obo/CHEBI_4909
T86 11317-11319 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T87 11338-11340 Chemical denotes SL http://purl.obolibrary.org/obo/CHEBI_74815
T88 11583-11590 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T89 11719-11726 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T90 11732-11734 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T91 11738-11740 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T92 12025-12032 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T93 12337-12351 Chemical denotes lead compounds http://purl.obolibrary.org/obo/CHEBI_33585
T94 12414-12423 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T95 12477-12487 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T96 12506-12513 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T97 12586-12596 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T98 12627-12637 Chemical denotes stabilizer http://purl.obolibrary.org/obo/CHEBI_77966
T99 12707-12714 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T100 12762-12770 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T101 12844-12846 Chemical denotes FL http://purl.obolibrary.org/obo/CHEBI_91150
T102 12884-12886 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T103 12913-12919 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T104 12940-12944 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T105 12945-12948 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T106 12969-12973 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T107 13120-13122 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T108 13142-13144 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T109 13283-13285 Chemical denotes TI http://purl.obolibrary.org/obo/CHEBI_141446
T110 13309-13311 Chemical denotes NA http://purl.obolibrary.org/obo/CHEBI_33696
T111 13370-13377 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T112 13461-13468 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T113 13529-13536 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T114 13647-13649 Chemical denotes Rg http://purl.obolibrary.org/obo/CHEBI_33368
T115 13658-13665 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T116 13752-13759 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T117 13763-13771 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T118 13876-13883 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T119 13935-13942 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T120 14100-14107 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T121 14215-14222 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T122 14342-14349 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T123 14570-14572 Chemical denotes FL http://purl.obolibrary.org/obo/CHEBI_91150
T124 14599-14606 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T125 14653-14659 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T126 14680-14684 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T127 14685-14688 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T128 14697-14701 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T129 14795-14803 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T130 15023-15030 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T131 15280-15287 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T132 15505-15507 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T133 15576-15583 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T134 15706-15713 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T135 15785-15792 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T136 15854-15862 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T137 15900-15908 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T138 15976-15984 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T139 16192-16198 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T140 16306-16313 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T141 16383-16390 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T142 16491-16498 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T143 16634-16641 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T144 17423-17425 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T145 17696-17703 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T146 17704-17713 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T147 17833-17840 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T148 17890-17897 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T149 18466-18475 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T150 18514-18521 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T151 18716-18723 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T152 18829-18836 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T153 18877-18884 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T154 18951-18958 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T155 19006-19014 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T156 19281-19288 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T157 19419-19428 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T158 19737-19739 Chemical denotes SE http://purl.obolibrary.org/obo/CHEBI_74813
T159 19869-19876 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T160 19925-19932 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T161 20032-20036 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T162 20183-20185 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T163 20187-20189 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T164 20222-20228 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T165 20260-20262 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T166 20301-20303 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T167 20670-20677 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T168 20866-20868 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T169 20969-20971 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T170 21066-21068 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T171 21101-21106 Chemical denotes anion http://purl.obolibrary.org/obo/CHEBI_22563
T172 21131-21143 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T173 21131-21138 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T174 21151-21153 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T175 21154-21162 Chemical denotes indoline http://purl.obolibrary.org/obo/CHEBI_43295
T176 21212-21217 Chemical denotes donor http://purl.obolibrary.org/obo/CHEBI_17891
T177 21218-21226 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T178 21250-21252 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T179 21253-21265 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T180 21253-21260 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T181 21311-21313 Chemical denotes 5B http://purl.obolibrary.org/obo/CHEBI_27560
T182 21328-21330 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T183 21454-21464 Chemical denotes amino acid http://purl.obolibrary.org/obo/CHEBI_33709
T184 21454-21459 Chemical denotes amino http://purl.obolibrary.org/obo/CHEBI_46882
T185 21460-21464 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527
T186 21700-21708 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T187 21713-21720 Chemical denotes ligands http://purl.obolibrary.org/obo/CHEBI_52214
T188 21796-21803 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T189 21938-21950 Chemical denotes benzene ring http://purl.obolibrary.org/obo/CHEBI_52879
T190 21938-21945 Chemical denotes benzene http://purl.obolibrary.org/obo/CHEBI_16716
T191 22128-22134 Chemical denotes cation http://purl.obolibrary.org/obo/CHEBI_36916
T192 22510-22519 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T193 23070-23080 Chemical denotes apoprotein http://purl.obolibrary.org/obo/CHEBI_13850
T194 23215-23217 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T195 23250-23252 Chemical denotes Fo http://purl.obolibrary.org/obo/CHEBI_42485
T196 23334-23336 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T197 23437-23439 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T198 23506-23508 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T199 23563-23565 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T200 24092-24101 Chemical denotes molecules http://purl.obolibrary.org/obo/CHEBI_25367
T201 24115-24118 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T203 24277-24280 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T205 24441-24444 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T207 24644-24653 Chemical denotes oligomers http://purl.obolibrary.org/obo/CHEBI_132554
T208 24719-24728 Chemical denotes macrolide http://purl.obolibrary.org/obo/CHEBI_25106
T209 24793-24809 Chemical denotes anticancer agent http://purl.obolibrary.org/obo/CHEBI_35610
T210 25016-25023 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T211 25024-25031 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T212 25036-25043 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T213 25044-25050 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T214 25313-25319 Chemical denotes ligand http://purl.obolibrary.org/obo/CHEBI_52214
T215 25344-25352 Chemical denotes molecule http://purl.obolibrary.org/obo/CHEBI_25367
T216 25554-25561 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T217 25708-25715 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T218 26017-26025 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T219 26045-26049 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T220 26118-26129 Chemical denotes amino acids http://purl.obolibrary.org/obo/CHEBI_33709
T221 26118-26123 Chemical denotes amino http://purl.obolibrary.org/obo/CHEBI_46882
T222 26124-26129 Chemical denotes acids http://purl.obolibrary.org/obo/CHEBI_37527
T223 26432-26434 Chemical denotes S4 http://purl.obolibrary.org/obo/CHEBI_29401
T224 26745-26747 Chemical denotes S5 http://purl.obolibrary.org/obo/CHEBI_29386
T225 26925-26928 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T227 27152-27159 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T228 27339-27347 Chemical denotes hydrogen http://purl.obolibrary.org/obo/CHEBI_49637
T229 27348-27357 Chemical denotes deuterium http://purl.obolibrary.org/obo/CHEBI_29237
T230 27367-27369 Chemical denotes MS http://purl.obolibrary.org/obo/CHEBI_73613
T231 27650-27658 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T232 27704-27711 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T233 27797-27805 Chemical denotes molecule http://purl.obolibrary.org/obo/CHEBI_25367
T234 28008-28012 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T235 28073-28087 Chemical denotes lead compounds http://purl.obolibrary.org/obo/CHEBI_33585
T236 28162-28164 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T237 28166-28168 Chemical denotes P2 http://purl.obolibrary.org/obo/CHEBI_33472
T238 28289-28297 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893
T239 28352-28354 Chemical denotes Co http://purl.obolibrary.org/obo/CHEBI_27638
T240 28473-28480 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T241 28525-28533 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T242 28636-28644 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T243 28731-28740 Chemical denotes histidine http://purl.obolibrary.org/obo/CHEBI_27570
T244 28925-28927 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T245 29087-29094 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T246 29126-29135 Chemical denotes isopropyl http://purl.obolibrary.org/obo/CHEBI_30353
T247 29165-29169 Chemical denotes IPTG http://purl.obolibrary.org/obo/CHEBI_61448
T248 29304-29310 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T249 29319-29323 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T250 29331-29335 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T251 29336-29339 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T252 29347-29356 Chemical denotes imidazole http://purl.obolibrary.org/obo/CHEBI_14434|http://purl.obolibrary.org/obo/CHEBI_16069
T254 29367-29371 Chemical denotes PMSF http://purl.obolibrary.org/obo/CHEBI_8102
T255 29523-29525 Chemical denotes Ni http://purl.obolibrary.org/obo/CHEBI_28112
T256 29526-29529 Chemical denotes NTA http://purl.obolibrary.org/obo/CHEBI_39054|http://purl.obolibrary.org/obo/CHEBI_44557
T258 29581-29587 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T259 29599-29608 Chemical denotes imidazole http://purl.obolibrary.org/obo/CHEBI_14434|http://purl.obolibrary.org/obo/CHEBI_16069
T261 29648-29655 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T262 29700-29704 Chemical denotes salt http://purl.obolibrary.org/obo/CHEBI_24866|http://purl.obolibrary.org/obo/CHEBI_26710
T264 29705-29711 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T265 30018-30025 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T266 30026-30034 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T267 30104-30112 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T268 30133-30149 Chemical denotes ammonium sulfate http://purl.obolibrary.org/obo/CHEBI_62946
T269 30133-30141 Chemical denotes ammonium http://purl.obolibrary.org/obo/CHEBI_28938
T270 30142-30149 Chemical denotes sulfate http://purl.obolibrary.org/obo/CHEBI_16189
T271 30156-30160 Chemical denotes NaBr http://purl.obolibrary.org/obo/CHEBI_63004
T272 30170-30173 Chemical denotes PEG http://purl.obolibrary.org/obo/CHEBI_46793
T273 30250-30258 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T274 30328-30336 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T275 30397-30399 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T276 30506-30514 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T277 30531-30533 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T278 30601-30603 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T279 30650-30655 Chemical denotes Light http://purl.obolibrary.org/obo/CHEBI_30212
T280 31015-31017 Chemical denotes MR http://purl.obolibrary.org/obo/CHEBI_74698
T281 31275-31282 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T282 31369-31383 Chemical denotes glutaraldehyde http://purl.obolibrary.org/obo/CHEBI_64276
T283 31516-31520 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T284 31521-31524 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T285 31587-31609 Chemical denotes sodium dodecyl sulfate http://purl.obolibrary.org/obo/CHEBI_8984
T286 31587-31593 Chemical denotes sodium http://purl.obolibrary.org/obo/CHEBI_26708
T287 31594-31609 Chemical denotes dodecyl sulfate http://purl.obolibrary.org/obo/CHEBI_23872
T288 31594-31601 Chemical denotes dodecyl http://purl.obolibrary.org/obo/CHEBI_23870
T289 31602-31609 Chemical denotes sulfate http://purl.obolibrary.org/obo/CHEBI_16189
T290 31610-31624 Chemical denotes polyacrylamide http://purl.obolibrary.org/obo/CHEBI_51135|http://purl.obolibrary.org/obo/CHEBI_53656|http://purl.obolibrary.org/obo/CHEBI_60766
T293 31646-31649 Chemical denotes SDS http://purl.obolibrary.org/obo/CHEBI_8984
T294 31683-31686 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T296 31710-31717 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T297 31766-31769 Chemical denotes PPI http://purl.obolibrary.org/obo/CHEBI_53266|http://purl.obolibrary.org/obo/CHEBI_60614
T299 31837-31840 Chemical denotes H37 http://purl.obolibrary.org/obo/CHEBI_34780
T300 31878-31882 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T301 31938-31942 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T302 32043-32050 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T303 32058-32065 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T304 32227-32234 Chemical denotes ligands http://purl.obolibrary.org/obo/CHEBI_52214
T305 32338-32340 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T306 32409-32415 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T307 32436-32440 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T308 32441-32444 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T309 32465-32469 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T310 32488-32495 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T311 32534-32540 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T312 32583-32593 Chemical denotes Tryptophan http://purl.obolibrary.org/obo/CHEBI_16828|http://purl.obolibrary.org/obo/CHEBI_27897
T314 32633-32635 Chemical denotes FP http://purl.obolibrary.org/obo/CHEBI_74750
T315 32688-32690 Chemical denotes Co http://purl.obolibrary.org/obo/CHEBI_27638
T316 32871-32877 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T317 32898-32902 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T318 32903-32906 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T319 32927-32931 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T320 32949-32951 Chemical denotes FP http://purl.obolibrary.org/obo/CHEBI_74750
T321 33004-33006 Chemical denotes Co http://purl.obolibrary.org/obo/CHEBI_27638
T322 33046-33053 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T323 33092-33098 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T324 33423-33437 Chemical denotes lead compounds http://purl.obolibrary.org/obo/CHEBI_33585
T325 33485-33496 Chemical denotes neutral red http://purl.obolibrary.org/obo/CHEBI_86370
T326 33605-33610 Chemical denotes drugs http://purl.obolibrary.org/obo/CHEBI_23888
T327 33667-33671 Chemical denotes drug http://purl.obolibrary.org/obo/CHEBI_23888
T328 33975-33982 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T329 34075-34082 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T330 34118-34124 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T331 34145-34149 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T332 34150-34153 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T333 34174-34178 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T334 34325-34333 Chemical denotes solution http://purl.obolibrary.org/obo/CHEBI_75958
T335 34354-34360 Chemical denotes quartz http://purl.obolibrary.org/obo/CHEBI_46727
T336 34398-34404 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T337 34862-34864 Chemical denotes Rg http://purl.obolibrary.org/obo/CHEBI_33368
T338 35282-35284 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T339 35370-35377 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T340 35383-35385 Chemical denotes ID http://purl.obolibrary.org/obo/CHEBI_141439
T341 35708-35710 Chemical denotes NP http://purl.obolibrary.org/obo/CHEBI_50803|http://purl.obolibrary.org/obo/CHEBI_53793|http://purl.obolibrary.org/obo/CHEBI_73425
T344 36201-36210 Chemical denotes phosphate http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474
T348 36676-36683 Chemical denotes agarose http://purl.obolibrary.org/obo/CHEBI_2511
T349 36752-36755 Chemical denotes CO2 http://purl.obolibrary.org/obo/CHEBI_16526
T350 36836-36850 Chemical denotes crystal violet http://purl.obolibrary.org/obo/CHEBI_41688
T351 37902-37914 Chemical denotes Triton X-100 http://purl.obolibrary.org/obo/CHEBI_9750
T352 37971-37977 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T353 38200-38205 Chemical denotes Fluor http://purl.obolibrary.org/obo/CHEBI_24061
T354 38294-38296 Chemical denotes MA http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325
T357 38344-38356 Chemical denotes phenylindole http://purl.obolibrary.org/obo/CHEBI_48559
T358 38358-38362 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T359 38486-38488 Chemical denotes AG http://purl.obolibrary.org/obo/CHEBI_27569|http://purl.obolibrary.org/obo/CHEBI_40618|http://purl.obolibrary.org/obo/CHEBI_73757
T362 38779-38781 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T363 38825-38832 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T364 38894-38896 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T365 38941-38950 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T366 38980-38982 Chemical denotes S4 http://purl.obolibrary.org/obo/CHEBI_29401
T367 38985-38994 Chemical denotes antiviral http://purl.obolibrary.org/obo/CHEBI_22587
T368 39054-39056 Chemical denotes S5 http://purl.obolibrary.org/obo/CHEBI_29386
T369 39245-39247 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T370 39300-39302 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T371 39502-39504 Chemical denotes P1 http://purl.obolibrary.org/obo/CHEBI_60949
T372 39771-39774 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T373 39845-39848 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T374 39858-39861 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T375 39985-39993 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893
T376 40021-40024 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T377 40034-40037 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T378 40098-40101 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T379 40111-40114 Chemical denotes Lin http://purl.obolibrary.org/obo/CHEBI_32386
T380 40496-40503 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T381 40504-40511 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T382 40717-40719 Chemical denotes MR http://purl.obolibrary.org/obo/CHEBI_74698

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T1 1097-1120 http://purl.obolibrary.org/obo/GO_0051259 denotes protein oligomerization
T2 1493-1509 http://purl.obolibrary.org/obo/GO_0016032 denotes viral infections
T3 2497-2520 http://purl.obolibrary.org/obo/GO_0051259 denotes protein oligomerization
T4 3201-3220 http://purl.obolibrary.org/obo/GO_0019083 denotes viral transcription
T5 3207-3220 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T6 3290-3313 http://purl.obolibrary.org/obo/GO_0051259 denotes protein oligomerization
T7 3677-3700 http://purl.obolibrary.org/obo/GO_0051259 denotes protein oligomerization
T8 4331-4354 http://purl.obolibrary.org/obo/GO_0051259 denotes protein oligomerization
T9 7886-7895 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T10 8080-8089 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T11 10684-10693 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T12 12025-12045 http://purl.obolibrary.org/obo/GO_0030164 denotes protein denaturation
T13 15169-15178 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T14 16756-16765 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T15 17748-17757 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T16 17809-17818 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T17 18327-18342 http://purl.obolibrary.org/obo/GO_0039703 denotes RNA replication
T18 19059-19068 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T19 25758-25766 http://purl.obolibrary.org/obo/GO_0007610 denotes behavior
T20 25819-25828 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T21 26842-26851 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T22 27358-27366 http://purl.obolibrary.org/obo/GO_0015297 denotes exchange
T23 29298-29303 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T24 33497-33503 http://purl.obolibrary.org/obo/GO_0098739 denotes uptake
T25 33497-33503 http://purl.obolibrary.org/obo/GO_0098657 denotes uptake
T26 35860-35875 http://purl.obolibrary.org/obo/GO_0016032 denotes Viral Infection
T27 37038-37059 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T28 37046-37059 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T29 38825-38843 http://purl.obolibrary.org/obo/GO_0050821 denotes protein sequesters
T30 38825-38843 http://purl.obolibrary.org/obo/GO_0045185 denotes protein sequesters

LitCovid-PD-HP

Id Subject Object Predicate Lexical cue hp_id
T1 2673-2693 Phenotype denotes respiratory distress http://purl.obolibrary.org/obo/HP_0002098
T2 2843-2852 Phenotype denotes pneumonia http://purl.obolibrary.org/obo/HP_0002090
T3 11604-11612 Phenotype denotes rigidity http://purl.obolibrary.org/obo/HP_0002063
T4 26622-26631 Phenotype denotes hepatitis http://purl.obolibrary.org/obo/HP_0012115
T5 36537-36546 Phenotype denotes agitation http://purl.obolibrary.org/obo/HP_0000713
T6 39029-39038 Phenotype denotes hepatitis http://purl.obolibrary.org/obo/HP_0012115
T7 40921-40930 Phenotype denotes hepatitis http://purl.obolibrary.org/obo/HP_0012115

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T1 0-134 Sentence denotes Structure-Based Stabilization of Non-native Protein–Protein Interactions of Coronavirus Nucleocapsid Proteins in Antiviral Drug Design
T2 136-144 Sentence denotes Abstract
T3 145-257 Sentence denotes Structure-based stabilization of protein–protein interactions (PPIs) is a promising strategy for drug discovery.
T4 258-392 Sentence denotes However, this approach has mainly focused on the stabilization of native PPIs, and non-native PPIs have received little consideration.
T5 393-570 Sentence denotes Here, we identified a non-native interaction interface on the three-dimensional dimeric structure of the N-terminal domain of the MERS-CoV nucleocapsid protein (MERS-CoV N-NTD).
T6 571-660 Sentence denotes The interface formed a conserved hydrophobic cavity suitable for targeted drug screening.
T7 661-892 Sentence denotes By considering the hydrophobic complementarity during the virtual screening step, we identified 5-benzyloxygramine as a new N protein PPI orthosteric stabilizer that exhibits both antiviral and N-NTD protein-stabilizing activities.
T8 893-1160 Sentence denotes X-ray crystallography and small-angle X-ray scattering showed that 5-benzyloxygramine stabilizes the N-NTD dimers through simultaneous hydrophobic interactions with both partners, resulting in abnormal N protein oligomerization that was further confirmed in the cell.
T9 1161-1320 Sentence denotes This unique approach based on the identification and stabilization of non-native PPIs of N protein could be applied toward drug discovery against CoV diseases.
T10 1322-1334 Sentence denotes Introduction
T11 1335-1456 Sentence denotes Small-molecule stabilization of protein–protein interactions (PPIs) is an extremely promising strategy in drug discovery.
T12 1457-1606 Sentence denotes It can be used to treat cancers and viral infections.1−3 Stabilizing PPIs with small molecules may be allosteric or direct (also called orthosteric).
T13 1607-2151 Sentence denotes This process alters the oligomerization equilibrium of the protein and enables small molecules to modulate its physiological function.4−7 The anticancer drug paclitaxel, for example, allosterically enhances microtubule structure assembly by binding to β-tubulin.8,9 On the other hand, rapamycin, another anticancer agent, binds directly to the interface between FKBP12 and mTOR and stabilizes the structure of the complex.10 The most well-characterized PPIs suitable as targets for drug development form natively under physiological conditions.
T14 2152-2295 Sentence denotes However, non-native interactions, which may form under extreme circumstances such as inside a crystal lattice, are also potential drug targets.
T15 2296-2551 Sentence denotes For example, nucleozin exerts its antiviral activity by stabilizing the non-native PPI interface between the two neighboring nucleoprotein trimers within the influenza virus, which results in abnormal protein oligomerization and loss of viral viability.11
T16 2552-2655 Sentence denotes Middle East respiratory syndrome coronavirus (MERS-CoV) belongs to the betacoronavirus (β-CoVs) family.
T17 2656-2990 Sentence denotes It causes severe respiratory distress with a high mortality rate in humans.12−14 Recently, a closely related novel coronavirus, coronavirus disease 2019 (COVID-19), caused an outbreak of pneumonia in Wuhan, which further underscored the risk of CoVs to the global public health.15,16 However, there is no effective treatment for CoVs.
T18 2991-3175 Sentence denotes Thus, there is an urgent need to develop new antiviral agents against CoVs.14,17 MERS-CoV packages its genome in a nucleocapsid (N) protein and forms a ribonucleoprotein (RNP) complex.
T19 3176-3234 Sentence denotes The RNP is essential for viral transcription and assembly.
T20 3235-3603 Sentence denotes Several studies suggested that the modulation of CoV N protein oligomerization by small molecules is a feasible antiviral drug development strategy.18,19 The CoV N protein is organized into the N-terminal domain (NTD) and the C-terminal domain (CTD), with both domains participating in RNA binding.20,21 All CoV N-NTD structures are folded in a monomeric conformation.
T21 3604-3739 Sentence denotes In contrast, the CoV N-CTDs are always dimeric and are responsible for N protein oligomerization via protein–protein interactions.22,23
T22 3740-3834 Sentence denotes Here, we report the crystal structure of MERS-CoV N-NTD in a non-native dimeric configuration.
T23 3835-3939 Sentence denotes We used the non-native dimer interface as the target in virtual screening for an orthosteric stabilizer.
T24 3940-4123 Sentence denotes To this end, we considered the binding scores and hydrophobic complementarity of the acquired poses, and further selected the potential leads P1–P3 from Acros and ZINC drug databases.
T25 4124-4226 Sentence denotes Of these, only 5-benzyloxygramine (P3) had both antiviral and stabilizing activities on the N protein.
T26 4227-4386 Sentence denotes Small-angle X-ray scattering (SAXS) and cell-based assays showed that P3 induces abnormal full-length N protein oligomerization in vitro and at the cell level.
T27 4387-4510 Sentence denotes We also described the structure of MERS-CoV N-NTD complexed with 5-benzyloxygramine and revealed its stabilizing mechanism.
T28 4511-4655 Sentence denotes Our findings provide insight into the development of a new therapeutic approach based on stabilizing a non-native protein interaction interface.
T29 4656-4751 Sentence denotes It may lead to the discovery and development of new treatments for various infectious diseases.
T30 4753-4760 Sentence denotes Results
T31 4762-4822 Sentence denotes Structure of the N-Terminal Domain of the MERS-CoV N Protein
T32 4823-4954 Sentence denotes We determined the crystal structure of MERS-CoV N-NTD by molecular replacement (MR) using the structure of HCoV-OC43 N-NTD (PDB ID:
T33 4955-5114 Sentence denotes 4J3K) as the search model.24 The final structure was refined to R-factor and R-free values of 0.26 and 0.29, respectively, at a resolution of 2.6 Å (Table S1).
T34 5115-5268 Sentence denotes Each asymmetric unit contained four N-NTD molecules assembled into two identical dimers with an overall RMSD of 0.28 Å between the dimers (Figure S1A,B).
T35 5269-5358 Sentence denotes The monomers shared a similar structural core preceded by a flexible region (Figure S1D).
T36 5359-5650 Sentence denotes The core consisted of a five-stranded antiparallel β-sheet sandwiched between loops arranged in a right-handed, fist-shaped structure conserved among the CoVs.25 In our structure, however, the loop connecting strands β2 and β3 protruding out of the core into other CoV N proteins was absent.
T37 5651-5755 Sentence denotes Unlike the reported structures that have a monomeric conformation, our structure was atypically dimeric.
T38 5756-5836 Sentence denotes Figure 1B shows the details of the interactions in the MERS-CoV N protein dimer.
T39 5837-5892 Sentence denotes We named the units monomer 1 and monomer 2 (Figure 1A).
T40 5893-6123 Sentence denotes According to the amino acid composition of the binding site on monomer 2, we divided the dimeric interface into two areas: one located on the N-terminus flexible region and the other on the loop between β4 and β5 of the N protein.
T41 6124-6331 Sentence denotes In the first area, W43, N66, N68, Y102, and F135 of monomer 1 generated a conserved hydrophobic pocket permitting the side chain of M38 of monomer 2 to enter this hole by a hydrophobic contact (Figure 1C,D).
T42 6332-6465 Sentence denotes H37 and N39 of monomer 2 were packed against W43 and F135 of monomer 1, respectively, and contributed to the hydrophobic interaction.
T43 6466-6585 Sentence denotes The side chains of N39 of monomer 2 formed one hydrogen bond with the N68 backbone in monomer 1 at a distance of 2.6 Å.
T44 6586-6634 Sentence denotes The second area was relatively more hydrophilic.
T45 6635-6823 Sentence denotes The main chain oxygens of G104, F135, and T137 of monomer 2 formed three hydrogen bonds with the side chains of Q73 and T134 of monomer 1 at distances of 3.8, 3.2, and 3.7 Å, respectively.
T46 6824-6954 Sentence denotes The side chain of N139 on monomer 2 formed a hydrogen bond with the main chain oxygen of T137 on monomer 1 at a distance of 3.6 Å.
T47 6955-7070 Sentence denotes The interactions of the first and second areas comprise buried surface areas (BSA) of 289 and 103 Å2, respectively.
T48 7071-7218 Sentence denotes The small surface area buried at the interface accounts for ∼5 kcal mol–1 binding energy,26 which translates to a dissociation constant of ∼200 mM.
T49 7219-7367 Sentence denotes Thus, the dimer described here is unique in that it is non-native and relies on vector-fusion residues (H37 and M38) to maintain its dimeric status.
T50 7368-7660 Sentence denotes This property may also explain why the present structure has an oligomeric status different from previously reported structures for the CoV N protein.24,27−30 We used cross-linking experiments to analyze the oligomeric capacity of MERS N-NTD containing the vector-fusion residues in solution.
T51 7661-7711 Sentence denotes MERS N-NTD had a dimeric conformation in solution.
T52 7712-7896 Sentence denotes Our structure indicated that W43 played an essential role in forming the hydrophobic pocket accommodating the vector-fusion residues and, therefore, mediated the N-NTD dimer formation.
T53 7897-7983 Sentence denotes The W43A mutation significantly reduced the oligomeric tendency of N-NTD (Figure S1C).
T54 7984-8114 Sentence denotes This further supports that the “exogenous residues” encoded by the vector backbone mediated the formation of the non-native dimer.
T55 8115-8197 Sentence denotes We also superimposed the previously published structure of MERS-CoV N-NTD (PDB ID:
T56 8198-8278 Sentence denotes 4ud1)27 containing a native N-terminal flexible region with our dimer structure.
T57 8279-8424 Sentence denotes The side chain of N38 in the native structure could not interact with the hydrophobic pocket as the former is hydrophilic and short (Figure S1E).
T58 8425-8570 Sentence denotes Thus, it may be possible to utilize small compounds to replace the vector-fusion residues and stabilize the PPI through hydrophobic interactions.
T59 8571-8769 Sentence denotes Figure 1 Structure and sequence of MERS-CoV N-NTD. (A) Overall structure of the MERS-CoV N-NTD dimer containing monomers 1 and 2 is depicted as a cartoon and colored yellow and green, respectively.
T60 8770-8887 Sentence denotes Residues involved in dimerization are shown as sticks and highlighted in (B). (B) Interactions among MERS-CoV N-NTDs.
T61 8888-9085 Sentence denotes Dimerization is mediated mainly by vector-fusion residues interacting with the conserved hydrophobic regions on the core structure (first area) along with the residues surrounding it (second area).
T62 9086-9171 Sentence denotes Interacting residues on monomers 1 and 2 are labeled in black and blue, respectively.
T63 9172-9259 Sentence denotes Vector-fusion and conserved hydrophobic regions are colored cyan and red, respectively.
T64 9260-9348 Sentence denotes The color of all other interacting residues is the same as that for each monomer in (A).
T65 9349-9479 Sentence denotes Polar contacts are indicated with red dashed lines. (C) Upper panel: close-up of the interacting region of vector-fusion residues.
T66 9480-9565 Sentence denotes The surface was colored according to the hydrophobicity level at the protein surface.
T67 9566-9653 Sentence denotes Vector-fusion residues (black) are shown as sticks to emphasize the hydrophobic pocket.
T68 9654-9666 Sentence denotes Lower panel:
T69 9667-9760 Sentence denotes 2D diagrams of the interaction between the hydrophobic pocket and the vector-fusion residues.
T70 9761-9793 Sentence denotes The latter are labeled in black.
T71 9794-9932 Sentence denotes The hydrophobic contacts are indicated with black dashed lines. (D) Sequence alignment of various CoV N proteins in the N-terminal region.
T72 9933-9982 Sentence denotes Red letters indicate strictly conserved residues.
T73 9983-10030 Sentence denotes Cyan indicates conservative substitution sites.
T74 10031-10117 Sentence denotes Hydrophobic regions involved in unusual dimerization are indicated by black triangles.
T75 10119-10193 Sentence denotes Direct Targeting of the Non-native Dimer Interface for Antiviral Screening
T76 10194-10317 Sentence denotes We performed a structure-based virtual screening by targeting W43 in the hydrophobic pocket of the N-NTD dimeric interface.
T77 10318-10505 Sentence denotes H37 and M38 were removed from the template to identify compounds that could replace the vector-fusion residues and, therefore, contribute to the stabilizing effect (Figures 2A and S2A,B).
T78 10506-10671 Sentence denotes We chose the highest-scoring hits, listed in Table S2, based on shape complementarity, the presence of aromatic moieties, and the ability to stack onto W43 of N-NTD.
T79 10672-11073 Sentence denotes Because the formation of the non-native dimers was primarily mediated by hydrophobic interactions in our structure (Figure 1C,D), we next considered the hydrophobic complementarity between the acquired ligands and N-NTD in the form of the lipophilic match surface (SL/L).31 We also took into account the ability of the drug to permeate cells by aiming for lower topological polar surface areas (TPSA).
T80 11074-11157 Sentence denotes Based on the above criteria, three compounds were finally chosen for further study.
T81 11158-11288 Sentence denotes Benzyl-2-(hydroxymethyl)-1-indolinecarboxylate (P1) and 5-benzyloxygramine (P3) had higher SL/L and docking scores and lower TPSA.
T82 11289-11369 Sentence denotes The clinical drug etodolac (P2) had a comparable SL/L but a lower docking score.
T83 11370-11405 Sentence denotes It too was selected as a candidate.
T84 11406-11788 Sentence denotes However, only P3 induced a comparatively larger blue shift in the intrinsic N-NTD fluorescence spectrum, indicating that the microenvironment surrounding the tryptophans of the protein increased in rigidity and hydrophobicity in the presence of P3.32 The result also indicated that P3 bound more tightly to the N protein than P1 or P2 by interacting with the W43 pocket (Figure 2B).
T85 11789-11933 Sentence denotes Fluorescent thermal stability assays disclosed that the N-NTD denaturation melting temperature had increased from 42 to 45 °C when P3 was added.
T86 11934-12011 Sentence denotes The sigmoidal melting curve for MERS-CoV N-NTD changed in the presence of P3.
T87 12012-12121 Sentence denotes The delay in protein denaturation suggests that P3 stabilized the MERS-CoV N-NTD dimer structure (Figure 2C).
T88 12122-12270 Sentence denotes We then measured the cytotoxic concentration (CC50) and effective concentration (EC50) for each compound using Vero E6 cells infected with MERS-CoV.
T89 12271-12373 Sentence denotes Table 1 shows that P3 had a favorable therapeutic index among the lead compounds tested in this study.
T90 12374-12441 Sentence denotes Therefore, P3 is an excellent candidate inhibitor against MERS-CoV.
T91 12442-12611 Sentence denotes Figure 2 Compound P3 was a potent stabilizer of the MERS-CoV N protein. (A) Schematic depicting the rationale used in designing the allosteric stabilizer of this study.
T92 12612-12974 Sentence denotes An orthosteric stabilizer may be used to bind to the non-native interaction interface of the N protein and stabilize the abnormal interaction between proteins. (B) Conformation and (C) stability analyses were performed based on the FL spectra of NTD (1 μM) incubated with P1–P3 (10 μM) for 1 h with a buffer consisting of 50 mM Tris-HCl (pH 8.3) and 150 mM NaCl.
T93 12975-13039 Sentence denotes Table 1 CC50 and EC50 and Therapeutic Indexes of Lead Compounds
T94 13040-13079 Sentence denotes quantal dose–response relationship (μM)
T95 13080-13119 Sentence denotes compound CC50a EC50b TIc (CC50/EC50)
T96 13120-13141 Sentence denotes P1 459.69 >100 NAd
T97 13142-13163 Sentence denotes P2 569.77 >100 NAd
T98 13164-13186 Sentence denotes P3 805.32 32.1 25.1
T99 13187-13195 Sentence denotes a CC50:
T100 13196-13232 Sentence denotes Half maximal toxicity concentration.
T101 13233-13241 Sentence denotes b EC50:
T102 13242-13279 Sentence denotes Half maximum effective concentration.
T103 13280-13286 Sentence denotes c TI:
T104 13287-13305 Sentence denotes Therapeutic index.
T105 13306-13312 Sentence denotes d NA:
T106 13313-13326 Sentence denotes Nonavailable.
T107 13328-13389 Sentence denotes Structural Model of P3-Induced MERS-CoV N Protein Aggregation
T108 13390-13479 Sentence denotes We used SAXS to assess the effects of P3 on the full-length MERS-CoV N protein structure.
T109 13480-13580 Sentence denotes The fitted distance distribution function of the protein with and without P3 are shown in Figure 3A.
T110 13581-13718 Sentence denotes P3 increased the maximum dimension (Dmax) and radius of gyration (Rg) of the protein from 207 to 230 Å and from 58 to 65 Å, respectively.
T111 13719-13803 Sentence denotes Thus, the size of the MERS-CoV N protein in solution was altered upon binding to P3.
T112 13804-14042 Sentence denotes Figure 3 P3-induced abnormal aggregation on the full-length MERS-CoV N protein. (A–E) SAXS analysis of the full-length MERS-CoV N protein. (A) Normalized results from GNOM showing pairwise distance distribution P(r) and maximum distance.
T113 14043-14428 Sentence denotes The radius of gyration fitted to 207 and 230 Å for the N protein and the N-P3 complex, respectively. “r” represents pairwise distances. (B, C) Scattering profiles of the N protein (B) and the N-P3 complex (C) and normalization fitting with GNOM (dashed lines). (D, E) Representative models of the N protein (D) and the N-P3 complex (E) generated by CRYSOL simulations of the SAXS data.
T114 14429-14454 Sentence denotes Only α carbons are shown.
T115 14455-14757 Sentence denotes NTD (yellow), CTD (green), and disorder region (cyan). (F, G) Conformation (F) and stability (G) analyses based on FL spectra of the MERS-CoV N protein (1 μM) incubated with P3 (10 μM) for 1 h in a buffer consisting of 50 mM Tris-HCl, 150 mM NaCl (pH 8.3). (H) Schematic of the P3 inhibition mechanism.
T116 14758-14875 Sentence denotes Left panel: in the absence of RNA, N proteins organize as a dimeric building block contributed by N-CTD dimerization.
T117 14876-15052 Sentence denotes Middle panel: P3 promoted the dimerization of N-NTDs from different building blocks, by which the distance between CTD cuboids was shortened and N protein aggregation occurred.
T118 15053-15152 Sentence denotes Right panel: octameric conformation of building blocks buried in the RNA-binding surface of N-CTDs.
T119 15153-15212 Sentence denotes It hindered the formation of filamentous ribonucleocapsids.
T120 15213-15358 Sentence denotes The presence of multiple intrinsically disordered regions in the N protein precluded the determination of its structure by X-ray crystallography.
T121 15359-15508 Sentence denotes Instead, we used rigid body modeling of the SAXS data with the N-terminal domain (NTD; solved in this study) and the C-terminal domains (CTD, PDB ID:
T122 15509-15622 Sentence denotes 6G13).23 In this way, we obtained structural models for the free N protein and its complex with P3 (Figure 3B,C).
T123 15623-15652 Sentence denotes Excellent fits were obtained.
T124 15653-15773 Sentence denotes Representative structural models for the full-length protein without and with P3 are shown in Figure 3D,E, respectively.
T125 15774-15875 Sentence denotes The free N protein formed a tetramer through CTD with the NTD freely hanging in solution (Figure 3D).
T126 15876-16076 Sentence denotes The conformation of the solution was consistent with structures previously reported for other CoV N proteins.33 The N-P3 complex formed a compact hexadecamer with a sunburst configuration (Figure 3E).
T127 16077-16175 Sentence denotes The CTDs formed a central ring and non-native NTD dimers formed “spikes” protruding from the ring.
T128 16176-16348 Sentence denotes Consistent with ligand-induced aggregation, we observed a “blue shift” in the fluorescence spectrum of the full-length MERS-CoV N protein in the presence of P3 (Figure 3F).
T129 16349-16551 Sentence denotes The addition of P3 also delayed N protein thermal denaturation and changed the shape of the denaturation curve, further suggesting that large protein aggregates formed in the presence of P3 (Figure 3G).
T130 16552-16627 Sentence denotes The structure explains how N-NTD dimerization decreased MERS-CoV viability.
T131 16628-16688 Sentence denotes The N protein packages the viral genome into an RNP complex.
T132 16689-16948 Sentence denotes Several models for N-CTD dimer assembly have been proposed for the formation of filamentous RNPs.33 All of the proposed interfaces between N-CTD dimers occurred on the side-faces of the CTD cuboid perpendicular to the proposed RNA-binding surface (Figure 3H).
T133 16949-17240 Sentence denotes Combinatorial use of any region on the side-faces of the CTD dimer cuboid may facilitate manipulation of the RNP length and curvature without obstructing the RNA-binding surface.28,34 However, the SAXS results indicated that N-CTD aggregation occurred on the β-sheet floor of the CTD cuboid.
T134 17241-17427 Sentence denotes For this reason, the RNA-binding surface of the CTD is occluded by the neighboring CTD on the ring and by the non-native NTD dimer making direct contact with the CTD (Figures 3H and S3).
T135 17428-17611 Sentence denotes In addition, the CTD cuboids in the aggregation naturally form a topologically closed octamer, leaving no open ends for further addition of CTD cuboids to form a long filamentous RNP.
T136 17612-17769 Sentence denotes Both the loss of the RNA-binding surface and the inability to incorporate further N protein molecules beyond an octamer may inhibit the formation of the RNP.
T137 17770-17853 Sentence denotes Therefore, P3 may inhibit MERS-CoV RNP formation by inducing N protein aggregation.
T138 17855-17909 Sentence denotes P3 Inhibits MERS-CoV by Inducing N Protein Aggregation
T139 17910-17990 Sentence denotes We demonstrated that P3 had the best characteristics as a therapeutic candidate.
T140 17991-18245 Sentence denotes To determine the anti-MERS-CoV activity of P3 in the cell, the effects of P3 incubation on extracellular viral titers and intracellular viral RNA levels were assessed by plaque assays on Vero E6 cells (Figure 4A) and by RT-qPCR (Figure 4B), respectively.
T141 18246-18350 Sentence denotes At 50 μM, P3 marginally affected the viral titer after 48 h but suppressed viral RNA replication by 40%.
T142 18351-18421 Sentence denotes At 100 μM, P3 halted both viral production and replication after 48 h.
T143 18422-18485 Sentence denotes This result proved the capacity of P3 as an antiviral compound.
T144 18486-18630 Sentence denotes We then examined MERS-CoV N protein distribution and expression in the infected cells with or without P3 treatment to confirm our SAXS findings.
T145 18631-18792 Sentence denotes Immunofluorescence microscopy (Figure 4C) showed condensation of the intracellular N protein fluorescence signal in infected Vero E6 cells treated with 50 μM P3.
T146 18793-18849 Sentence denotes Thus, P3 may induce intracellular N protein aggregation.
T147 18850-18910 Sentence denotes At 100 μM, P3 suppressed N protein expression in most cells.
T148 18911-18967 Sentence denotes However, a few presented with intense N protein signals.
T149 18968-19111 Sentence denotes P3 may have restrained the MERS-CoV N proteins inside the infected cells that promoted the formation of new virions that could not be released.
T150 19112-19180 Sentence denotes In this way, the adjacent cells could not be infected with MERS-CoV.
T151 19181-19305 Sentence denotes The data, therefore, suggest that P3 may inhibit MERS-CoV by inducing abnormal aggregation of the N protein inside the cell.
T152 19306-19380 Sentence denotes This finding is consistent with the results of our structure-based assays.
T153 19381-19585 Sentence denotes Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h.
T154 19586-19665 Sentence denotes Relative RNA levels were determined by comparing MERS alone at each time point.
T155 19666-19701 Sentence denotes GAPDH RNA was the internal control.
T156 19702-19765 Sentence denotes All values are presented as mean ± SE (standard error of mean).
T157 19766-19911 Sentence denotes One-way Anova was used for statistics (*p < 0.05, **p < 0.01, ***p < 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment.
T158 19912-20001 Sentence denotes Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680.
T159 20002-20037 Sentence denotes Nuclei were stained blue with DAPI.
T160 20039-20106 Sentence denotes Crystal Structure of MERS-CoV N-NTD Complexed with Potent Compounds
T161 20107-20237 Sentence denotes We attempted to obtain crystals of MERS-CoV N-NTD in complex with compounds P1, P2, and P3 by cocrystallization or ligand-soaking.
T162 20238-20382 Sentence denotes With the exception of P2, the complex structures of N-NTD with P1 and P3 were solved at resolutions of 3.09 and 2.77 Å, respectively (Table S1).
T163 20383-20454 Sentence denotes The overall structures of the complexes resembled that of apo-MERS-CoV.
T164 20455-20638 Sentence denotes Both complexes revealed well-defined unbiased densities in the dimer interface and permitted detailed analysis of the interactions between the compounds and MERS-CoV N-NTD (Figure 5).
T165 20639-20761 Sentence denotes The interactions between the N protein and each compound were calculated with the Discovery Studio Client (v19.1.0.18287).
T166 20762-20858 Sentence denotes Most interactions were hydrophobic contacts, which were consistent with our selection rationale.
T167 20859-21002 Sentence denotes In the P1 complex, N68, F135, and D143 on monomer 1 and V41, G106, P107, and T137 on monomer 2 packed against P1 to create a dimer (Figure 5A).
T168 21003-21086 Sentence denotes In addition, two nonbonding interactions were detected between P1 and the monomers.
T169 21087-21192 Sentence denotes There was a π-anion interaction between the benzene ring of the P1 indoline moiety and D143 of monomer 1.
T170 21193-21315 Sentence denotes There was also a π-donor hydrogen bond between the other P1 benzene ring and the T137 side chain of monomer 2 (Figure 5B).
T171 21316-21449 Sentence denotes Relative to P1, P3 bound more deeply into the dimer interface and interacted with a larger number of residues on both N-NTD monomers.
T172 21450-21611 Sentence denotes The amino acid composition of this binding region was W43, N66, N68, S69, T70, N73, and F135 on monomer 1 and V41, G104, T105, G106, A109, and T137 on monomer 2.
T173 21612-21816 Sentence denotes These residues along with P3 generated a massive hydrophobic driving force allowing the proteins and ligands to pack against each other and stabilize the dimeric conformation of the N protein (Figure 5C).
T174 21817-21891 Sentence denotes Several nonbonding interactions were also observed at the P3-binding site.
T175 21892-22035 Sentence denotes These included the interaction between the P3 benzene ring and N68 of monomer 1 and A109 of monomer 2 via π-lone pair and π-alkyl interactions.
T176 22036-22119 Sentence denotes The dimethylaminomethyl moiety of P3 was a major source of nonbonding interactions.
T177 22120-22228 Sentence denotes Three π-cation interactions formed between this moiety and the aromatic groups of W43 and F135 in monomer 1.
T178 22229-22348 Sentence denotes This moiety also formed a π-lone pair interaction with N66 and a π-sigma interaction with W43 of monomer 1 (Figure 5D).
T179 22349-22558 Sentence denotes The structural analyses explain the comparatively stronger binding of P3 to N-NTD (Figure 2B) and corroborated the thermal stabilization effects (Figure 2C) and antiviral activities (Table 1) of the compounds.
T180 22559-22630 Sentence denotes Figure 5 Structures of MERS-CoV N-NTD complexed with potent compounds.
T181 22631-23119 Sentence denotes The structures were solved using HCoV-OC43 N-NTD (PDB:4J3K) as the search model.24 Left panel: (Upper) structural superimposition of the MERS-CoV N-NTD:P1 complex (monomers 1 and 2 are in purple and pink, respectively) and the MERS-CoV N-NTD:P3 complex (monomers 1 and 2 are in brown and green, respectively) with compounds depicted as stick structures. (Lower) Interactions involving vector-fusion residues in the non-native dimer of the apoprotein shown for comparison with (A) and (B).
T182 23120-23154 Sentence denotes Color is the same as in Figure 1A.
T183 23155-23239 Sentence denotes Right panel: detailed interactions among MERS-CoV N-NTD and P1 (A, B) and P3 (C, D).
T184 23240-23350 Sentence denotes Different Fo–Fc maps were contoured at ∼2.5 σ. (A) Detailed stereoview of interactions at the P1-binding site.
T185 23351-23410 Sentence denotes The color of each monomer is the same as in the left panel.
T186 23411-23533 Sentence denotes Residues constructing the P1-binding pocket are labeled and showed as sticks. (B) Schematic of P1 bound to MERS-CoV N-NTD.
T187 23534-23613 Sentence denotes Hydrophobic contacts between P1 and each monomer are displayed as dashed lines.
T188 23614-23731 Sentence denotes Nonbonding interactions are indicated by cyan arrows. (C) Detailed stereoview of interactions at the P3-binding site.
T189 23732-23791 Sentence denotes The color of each monomer is the same as in the left panel.
T190 23792-23913 Sentence denotes Residues belonging to the P3-binding pocket are labeled and shown as sticks. (D) Schematic of P3 bound to MERS-CoV N-NTD.
T191 23914-23993 Sentence denotes Hydrophobic contacts between P3 and each monomer are displayed as dashed lines.
T192 23994-24046 Sentence denotes Nonbonding interactions are indicated by red arrows.
T193 24048-24074 Sentence denotes Discussion and Conclusions
T194 24075-24209 Sentence denotes The use of small molecules to stabilize PPI interfaces has been shown to be a viable approach for the development of new therapeutics.
T195 24210-25201 Sentence denotes Most compounds created by structure-based design to manipulate the PPI depend on detailed knowledge of the native interacting interfaces.35−37 In contrast, most of the compounds with therapeutic potential that stabilize non-native PPI interfaces were discovered by chance alone.38 The anti-influenza compound nucleozin was initially discovered using a chemical genetics approach, and its ability to stabilize non-native nucleoprotein oligomers was elucidated much later.39,40 Swinholide A, a cytotoxic marine macrolide, has been known to disrupt the actin cytoskeleton and act as an anticancer agent, but it took ten years to discover that it stabilized G-actin as a non-native homodimer complex.41−43 However, these examples did underscore the importance of hydrophobicity as a crucial factor stabilizing protein–protein and protein–ligand associations.44 Here, we demonstrated the possibility of using the hydrophobic interactions on non-native interfaces as targets for virtual screening.
T196 25202-25433 Sentence denotes Combined with suitable selection criteria focusing on both shape and hydrophobic complementarities between the ligand and the receptor, small-molecule compounds that stabilize non-native PPIs may be identified in a rational manner.
T197 25434-25637 Sentence denotes Using the above approach, we successfully identified a compound P3 that affected the biochemical activity of our target protein and showed efficacy against our target pathogen MERS-CoV (Figures 2 and 4).
T198 25638-25829 Sentence denotes P3-mediated non-native MERS-CoV N-NTD dimerization induced abnormal N protein aggregation by influencing the oligomeric behavior of N-CTD and eventually halting its function in RNP formation.
T199 25830-25973 Sentence denotes To the best of our knowledge, this structure-based strategy for targeting non-native interfaces has never been proposed for therapeutic design.
T200 25974-26075 Sentence denotes Thus, non-native interaction interfaces in proteins may comprise a new drug development target class.
T201 26076-26212 Sentence denotes For β-coronaviruses such as MERS-CoV, the amino acids comprising the non-native interaction interface on N-NTD are relatively conserved.
T202 26213-26436 Sentence denotes The hydrophobic pocket surrounding W43 and F135 on monomer 1 is shared among other β-coronaviruses.24,28 The interacting surface on monomer 2, which includes G104, T105, G106, and A109, is also highly conserved (Figure S4).
T203 26437-26594 Sentence denotes This conservation may provide certain advantages when developing compounds with broad-spectrum activity against a target pathogen family, including COVID-19.
T204 26595-26749 Sentence denotes When we tested P3 on mouse hepatitis virus (MHV), we observed a reduction in viral titer, indicating that P3 may also inhibit MHV replication (Figure S5).
T205 26750-26828 Sentence denotes On the other hand, targeting non-native interaction interfaces is not trivial.
T206 26829-27000 Sentence denotes As interface formation is induced by an external agent, computations aimed at predicting native PPI structures may not be able to identify potential non-native interfaces.
T207 27001-27114 Sentence denotes Nevertheless, several stratagems may assist in the identification of potential non-native interaction interfaces.
T208 27115-27237 Sentence denotes One strategy is to search for target protein structures with crystal packing contacts known to be biologically irrelevant.
T209 27238-27564 Sentence denotes Another approach is to identify weakly interacting sites through NMR chemical shift perturbation and hydrogen-deuterium exchange MS.45 Once these potential non-native interaction interfaces are identified, standard screening and then functional characterization may be conducted for small compounds that bind to the interface.
T210 27565-27682 Sentence denotes To summarize, we demonstrated that non-native interaction interfaces might form when proteins abnormally oligomerize.
T211 27683-27833 Sentence denotes Using the MERS-CoV N protein as an example, we showed that non-native interfaces might serve as targets for small-molecule, structure-based screening.
T212 27834-27946 Sentence denotes We also proposed several stratagems to rationally identify potential non-native interface targets for screening.
T213 27947-28114 Sentence denotes We believe that we have discovered and tested an alternative drug discovery paradigm that could help expand the repertoire of lead compounds against various pathogens.
T214 28116-28136 Sentence denotes Experimental Section
T215 28138-28147 Sentence denotes Chemicals
T216 28148-28284 Sentence denotes The compounds P1, P2, and P3 were purchased from Maybridge Chemical Company, TCI Chemicals, and Sigma-Aldrich Corporation, respectively.
T217 28285-28372 Sentence denotes The reagents used in this study were purchased from Sigma Chemical Co. (St. Louis, MO).
T218 28373-28462 Sentence denotes The purity of all compounds is higher than 95% and was used without further purification.
T219 28464-28509 Sentence denotes Cloning, Protein Expression, and Purification
T220 28510-28763 Sentence denotes The MERS-CoV N proteins were prepared according to previously described methods.46 In brief, the cDNA fragments of MERS-CoV N proteins were cloned into a pET-28a expression vector (Merck, Darmstadt, Germany) containing a histidine tag-encoding sequence.
T221 28764-28928 Sentence denotes Vectors encoding the single mutants N39A, N39G, and W43A were generated using the QuikChange site-directed mutagenesis protocol with the primers listed in Table S3.
T222 28929-29003 Sentence denotes The vectors were transformed into Escherichia coli BL21 (DE3) pLysS cells.
T223 29004-29213 Sentence denotes The cells were grown to an optical density range of 0.6–0.8 at 600 nm at 37 °C and protein expression induced with 1.0 mM isopropyl β-d-1-thiogalactopyranoside (IPTG), followed by incubation at 10 °C for 24 h.
T224 29214-29381 Sentence denotes The cells were harvested by centrifugation (6000g, 12 min, 4 °C) and resuspended in lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 15 mM imidazole, and 1 mM PMSF; pH 7.5).
T225 29382-29473 Sentence denotes The cells were lysed by sonication and centrifuged (10000g, 40 min, 4 °C) to remove debris.
T226 29474-29642 Sentence denotes The supernatant was purified by injection into a Ni–NTA column (Merck, Darmstadt, Germany) and eluted with buffer containing imidazole at a gradient range of 15–300 mM.
T227 29643-29811 Sentence denotes Pure protein fractions were collected, dialyzed with low-salt buffer, concentrated, and quantified by the Bradford method (BioShop Canada Inc., Burlington, ON, Canada).
T228 29813-29848 Sentence denotes Crystallization and Data Collection
T229 29849-30015 Sentence denotes MERS-CoV N-NTD crystals were grown as previously described:46 MERS-CoV N-NTD was crystallized at room temperature (∼25 °C) by the sitting-drop vapor-diffusion method.
T230 30016-30259 Sentence denotes A protein solution (2 μL; 10 mg mL–1) was mixed with an equal volume of crystallization solution consisting of 75 mM ammonium sulfate, 2 mM NaBr, and 29% PEG 3350 (Sigma-Aldrich Corp., St. Louis, MO) and equilibrated against a 300 μL solution.
T231 30260-30356 Sentence denotes MERS-CoV N-NTD:P3 co-crystals were obtained using a crystallization solution containing 2 mM P3.
T232 30357-30534 Sentence denotes MERS-CoV N-NTD crystals in complex with P1 were obtained by soaking native MERS-CoV N-NTD crystals for 90 s at room temperature in a crystallization solution containing 2 mM P1.
T233 30535-30743 Sentence denotes Diffraction datasets for MERS-CoV N-NTD alone and in complex with P1 were collected at beamline 13B1 of the Taiwan Light Source (TLS) of the National Synchrotron Research Center (NSRRC; Hsinchu City, Taiwan).
T234 30744-30853 Sentence denotes Diffraction of the MERS-CoV N-NTD:P3 complex was performed at the beamline SP44XU of SPring-8 (Hyogo, Japan).
T235 30855-30894 Sentence denotes Structural Determination and Refinement
T236 30895-30961 Sentence denotes Diffraction data were processed and scaled with HKL-2000 software.
T237 30962-31152 Sentence denotes The structures were solved by molecular replacement (MR) in Phenix47 using HCoV-OC43 N-NTD (PDB:4J3K) as the search model.24 The initial models were rebuilt and refined by Coot48 and Phenix.
T238 31153-31247 Sentence denotes Structures were visualized using PyMOL (The PyMOL Molecular Graphics System, version 2.3.0).49
T239 31249-31274 Sentence denotes Chemical Cross-Link Assay
T240 31275-31419 Sentence denotes Protein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v.
T241 31420-31534 Sentence denotes The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5).
T242 31535-31656 Sentence denotes The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
T243 31658-31717 Sentence denotes Discovery of Orthosteric PPI Stabilizers for MERS N Protein
T244 31718-31893 Sentence denotes To screen for compounds that induce hydrophobic PPI between MERS N-NTDs, a model of dimeric MERS-CoV N-NTD without the H37 and M38 residues was used in virtual drug screening.
T245 31894-32051 Sentence denotes The Sigma-Aldrich, Acros Organics, and ZINC drug databases were screened with LIBDOCK molecular docking software to obtain compounds acting on the N protein.
T246 32052-32117 Sentence denotes The N protein binding pocket was represented by a set of spheres.
T247 32118-32186 Sentence denotes Each compound in the database was docked in a pocket comprising W43.
T248 32187-32341 Sentence denotes The hydrophobic complementarity between ligands and receptors was calculated with PLATINUM.31 Compounds with higher docking scores are listed in Table S2.
T249 32343-32368 Sentence denotes Fluorescence Measurements
T250 32369-32470 Sentence denotes Fluorescence assays were performed in a buffer consisting of 50 mM Tris-HCl (pH 8.3) and 150 mM NaCl.
T251 32471-32582 Sentence denotes One micromolar N protein was incubated either with the control buffer or each compound (10 μM) at 4 °C for 1 h.
T252 32583-32691 Sentence denotes Tryptophan fluorescence was acquired with a Jasco FP-8300 fluorescence spectrometer (JASCO International Co.
T253 32692-32797 Sentence denotes Ltd., Tokyo, Japan) at an excitation wavelength of 280 nm and an emission wavelength range of 300–400 nm.
T254 32799-32827 Sentence denotes Thermostability Measurements
T255 32828-33007 Sentence denotes Thermostability assays were conducted in a buffer consisting of 50 mM Tris-HCl (pH 7.5) and 150 mM NaCl and with a JASCO FP-8300 fluorescence spectrometer (JASCO International Co.
T256 33008-33028 Sentence denotes Ltd., Tokyo, Japan).
T257 33029-33140 Sentence denotes One micromolar N protein was incubated either with the control buffer or each compound (10 μM) at 4 °C for 2 h.
T258 33141-33302 Sentence denotes UV absorbance vs temperature profiles were acquired by ramping the temperature from 4–95 °C at a 1 °C min–1 and recording the absorbance at 280 nm every 0.5 min.
T259 33304-33346 Sentence denotes Determining CC50 and EC50 of Hit Compounds
T260 33347-33447 Sentence denotes Vero E6 cells were infected with MERS-CoV with M.O.I = 0.1 and treated with lead compounds for 48 h.
T261 33448-33510 Sentence denotes Cell viability was determined by the neutral red uptake assay.
T262 33511-33561 Sentence denotes CC50 and EC50 were determined by % cell viability.
T263 33562-33616 Sentence denotes CC50 was determined for cells treated with drugs only.
T264 33617-33683 Sentence denotes EC50 was determined for MERS-infected cells after drug treatments.
T265 33685-33732 Sentence denotes Small-Angle X-ray Scattering (SAXS) Experiments
T266 33733-33956 Sentence denotes SAXS experiments were performed at the BL23A SAXS beamline at the TLS of NSRRC, using a monochromatic X-ray beam (λ = 0.828 Å), with an integrated HPLC system of an Agilent-Bio SEC-3 300 Å column (Agilent Technologies, Inc.
T267 33957-33974 Sentence denotes Santa Clara, CA).
T268 33975-34194 Sentence denotes Protein samples (44 μM MERS-CoV N and MERS-CoV N:P3 complex prepared by incubating the 44 μM native protein with 440 μM P3) were prepared in a buffer consisting of 50 mM Tris-HCl (pH 8.5) and 150 mM NaCl on ice for 1 h.
T269 34195-34279 Sentence denotes Then, a 100 μL aliquot was injected into the column at a flow rate of 0.02 mL min–1.
T270 34280-34442 Sentence denotes After passing through the column, the sample solution was directed into a quartz capillary (2 mm dia.) for subsequent buffer and sample SAXS measurement at 288 K.
T271 34443-34542 Sentence denotes The sample-to-detector distance of 2.5 m used covered a scattering vector q range of 0.01–0.20 Å–1.
T272 34543-34612 Sentence denotes Here, q is defined as q = (4π/λ) sin θ, with the scattering angle 2θ.
T273 34613-34712 Sentence denotes Thirty-six frames were collected for each sample elution with an X-ray frame exposure time of 30 s.
T274 34713-34892 Sentence denotes Frames of good data overlapping (namely, of low radiation damage effects) were merged for improved data statistics and analyzed to determine initial Rg using PRIMUS (version 3.1).
T275 34893-35013 Sentence denotes The P(r) distance distribution and Dmax were calculated from the experimental scattering curve using GNOM (version 4.1).
T276 35014-35227 Sentence denotes An ensemble optimization method (EOM) analysis was performed through the EMBL Hamburg web interface.50 Modeling of the rigid body crystal structure was calculated and generated using CRYSOL (ATSAS Program Suite v.
T277 35228-35285 Sentence denotes 2.8.2).51 The crystal structures of MERS-CoV NTD (PDB ID:
T278 35286-35386 Sentence denotes 4UD1)27 and MERS-CoV NTD:P3 (solved in this study) and the CTD domain of MERS-CoV N protein (PDB ID:
T279 35387-35437 Sentence denotes 6G13)23 were used as rigid bodies in EOM analysis.
T280 35438-35526 Sentence denotes With the EOM analysis, 1000 models were generated in the beginning as a structural pool.
T281 35527-35687 Sentence denotes Selected from the SAXS profiles of the structural pool was an ensemble of models that could fit the experimental scattering curve with their linear combination.
T282 35688-35858 Sentence denotes Tetrameric MERS-CoV NP conformations and 16-mer MERS-CoV:P3 conformations were selected because their ensemble generated curves fit best to the experimental SAXS results.
T283 35860-35875 Sentence denotes Viral Infection
T284 35876-36041 Sentence denotes Vero E6 cells (ATCC No: CRL-1586) were seeded onto culture plates with complete Dulbecco’s modified Eagle’s medium (DMEM) and incubated overnight prior to infection.
T285 36042-36264 Sentence denotes MERS-CoV (HCoV-EMC/2012) at a multiplicity of infection (M.O.I.) of 0.1 was added to the cells and incubated at 37 °C for 1 h, followed by washing thrice with phosphate-buffered saline (PBS) to remove the unattached virus.
T286 36265-36324 Sentence denotes Fresh complete culture medium was then added to the plates.
T287 36326-36338 Sentence denotes Plaque Assay
T288 36339-36425 Sentence denotes Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays.
T289 36426-36560 Sentence denotes Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min.
T290 36561-36624 Sentence denotes After incubation, the inocula were removed and washed with PBS.
T291 36625-36767 Sentence denotes An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days.
T292 36768-36881 Sentence denotes The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted.
T293 36883-36890 Sentence denotes RT-qPCR
T294 36891-37037 Sentence denotes Total RNA of infected Vero E6 cells was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
T295 37038-37178 Sentence denotes Reverse transcription and PCR amplification were performed with an iTaq Universal One-Step RT-qPCR Kit (Bio-Rad Laboratories, Hercules, CA).
T296 37179-37283 Sentence denotes Real-time PCR was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA).
T297 37284-37356 Sentence denotes The primer pairs used to amplify the viral RNA were as follows: GAPDH-F:
T298 37357-37392 Sentence denotes 5′-GAAGGTGAAGGTCGGAGTC-3′; GAPDH-R:
T299 37393-37434 Sentence denotes 5′-GAAGATGGTGATGGGATTTC-3′;53 MERS-CoV-F:
T300 37435-37475 Sentence denotes 5′-CCACTACTCCCATTTCGTCAG-3′; MERS-CoV-R:
T301 37476-37631 Sentence denotes 5′-CAGTATGTGTAGTGCGCATATAAGCA-3′.54 The MERS RNA levels were normalized to that of GAPDH and compared between MERS-CoV groups at 24 h.p.i. and at 48 h.p.i.
T302 37633-37657 Sentence denotes Immunofluorescence Assay
T303 37658-37786 Sentence denotes Vero E6 cells were seeded in eight-well chamber slides and incubated overnight prior to infection with MERS-CoV at M.O.I. = 0.1.
T304 37787-37926 Sentence denotes The cells were fixed with 4% (v/v) paraformaldehyde for 20 min at 4 °C, followed by permeabilization in 0.1% (v/v) Triton X-100 for 10 min.
T305 37927-37998 Sentence denotes Then, 7.5% (v/v) BSA was used as a blocking buffer for 30 min at 37 °C.
T306 37999-38115 Sentence denotes Anti-MERS-CoV N primary antibody (1:500 dilution; Sino Biological Inc., Beijing, China) was used to stain the virus.
T307 38116-38326 Sentence denotes The cells were incubated overnight, washed thrice with PBS, and incubated with Alex Fluor 568 anti-rabbit secondary antibody (1:1000 dilution; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature.
T308 38327-38394 Sentence denotes 4′,6-Diamidino-2-phenylindole (DAPI) was added during the PBS wash.
T309 38395-38511 Sentence denotes MERS nucleocapsid expression was examined under a confocal microscope (LSM-700; Carl Zeiss AG, Oberkochen, Germany).
T310 38513-38545 Sentence denotes Supporting Information Available
T311 38546-39336 Sentence denotes The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jmedchem.9b01913.Dimeric conformation of MERS-CoV N-NTD (Figure S1); LibDock pose of dimeric MERS-CoV N-NTD with potent compounds (Figure S2); P3-induced aggregation of the MERS-CoV N protein sequesters the RNA-binding region on the N-CTD dimer (Figure S3); potential target sites for broad-spectrum antiviral compound development (Figure S4); antiviral activity of P3 against MHV (mouse hepatitis virus) (Figure S5); crystallographic data collection and refinement statistics (Table S1); pW43 docking pose with chemical structures, docking scores, and biochemical properties of 17 potential hits (Table S2); primers used for mutagenesis in this study (Table S3); molecular formula strings (PDF)
T312 39337-39381 Sentence denotes Molecular Formula Strings_JMC_revision (CSV)
T313 39383-39405 Sentence denotes Supplementary Material
T314 39406-39426 Sentence denotes jm9b01913_si_001.pdf
T315 39427-39447 Sentence denotes jm9b01913_si_002.csv
T316 39449-39464 Sentence denotes Accession Codes
T317 39465-39631 Sentence denotes For PDB codes 6KL2 (native), 6KL5 (N-P1 complex), and 6KL6 (N-P3 complex), authors will release the atomic coordinates and experimental data upon article publication.
T318 39633-39653 Sentence denotes Author Contributions
T319 39654-39728 Sentence denotes ○ S.-M.L., S.-C.L., J.-N.H., and C.-k.C. contributed equally to this work.
T320 39730-39750 Sentence denotes Author Contributions
T321 39751-39757 Sentence denotes Jia N.
T322 39758-39770 Sentence denotes Hsu, Shih C.
T323 39771-39783 Sentence denotes Lin, Yong S.
T324 39784-39801 Sentence denotes Wang, and Ming H.
T325 39802-39831 Sentence denotes Hou designed research; Jia N.
T326 39832-39844 Sentence denotes Hsu, Shih C.
T327 39845-39857 Sentence denotes Lin, Shan M.
T328 39858-39871 Sentence denotes Lin, Ching M.
T329 39872-39890 Sentence denotes Chien, and Yong S.
T330 39891-39923 Sentence denotes Wang performed research; Hung Y.
T331 39924-39932 Sentence denotes Wu, U S.
T332 39933-39941 Sentence denotes Jeng, K.
T333 39942-39964 Sentence denotes Kehn-Hall, and Ming H.
T334 39965-40020 Sentence denotes Hou contributed new reagents or analytic tools; Shih C.
T335 40021-40033 Sentence denotes Lin, Shan M.
T336 40034-40047 Sentence denotes Lin, Ching M.
T337 40048-40066 Sentence denotes Chien, and Ming H.
T338 40067-40097 Sentence denotes Hou analyzed the data; Shih C.
T339 40098-40110 Sentence denotes Lin, Shan M.
T340 40111-40124 Sentence denotes Lin, Chung K.
T341 40125-40140 Sentence denotes Chang, Ching M.
T342 40141-40159 Sentence denotes Chien, and Ming H.
T343 40160-40180 Sentence denotes Hou wrote the paper.
T344 40182-40241 Sentence denotes This work was supported by the MOST 106-2628-M-005-001-MY3.
T345 40243-40295 Sentence denotes The authors declare no competing financial interest.
T346 40297-40312 Sentence denotes Acknowledgments
T347 40313-40476 Sentence denotes We thank the National Synchrotron Radiation Research Center (Taiwan) and the synchrotron radiation facility SPring-8 (Japan) for X-ray diffraction data collection.
T348 40477-40490 Sentence denotes Abbreviations
T349 40491-40524 Sentence denotes PPIs protein–protein interactions
T350 40525-40578 Sentence denotes MERS-CoV Middle East Respiratory Syndrome Coronavirus
T351 40579-40600 Sentence denotes NTD N-terminal domain
T352 40601-40622 Sentence denotes CTD C-terminal domain
T353 40623-40637 Sentence denotes N nucleocapsid
T354 40638-40660 Sentence denotes β-CoVs betacoronavirus
T355 40661-40682 Sentence denotes RNP ribonucleoprotein
T356 40683-40716 Sentence denotes SAXS small-angle X-ray scattering
T357 40717-40741 Sentence denotes MR molecular replacement
T358 40742-40766 Sentence denotes BSA buried surface areas
T359 40767-40803 Sentence denotes TPSA topological polar surface areas
T360 40804-40832 Sentence denotes CC50 cytotoxic concentration
T361 40833-40861 Sentence denotes EC50 effective concentration
T362 40862-40886 Sentence denotes D max maximum dimension
T363 40887-40910 Sentence denotes R g radius of gyration
T364 40911-40936 Sentence denotes MHV mouse hepatitis virus

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id tao:has_standard_notation
1 76-87 Species denotes Coronavirus Tax:11118
8 523-531 Species denotes MERS-CoV Tax:1335626
9 554-562 Species denotes MERS-CoV Tax:1335626
10 757-775 Chemical denotes 5-benzyloxygramine MESH:C006736
11 960-978 Chemical denotes 5-benzyloxygramine MESH:C006736
12 994-999 Chemical denotes N-NTD
13 1307-1319 Disease denotes CoV diseases MESH:C000657245
22 1969-1975 Gene denotes FKBP12 Gene:2285
23 1980-1984 Gene denotes mTOR Gene:2475
24 2454-2469 Species denotes influenza virus Tax:11309
25 1765-1775 Chemical denotes paclitaxel MESH:D017239
26 1892-1901 Chemical denotes rapamycin MESH:D020123
27 2309-2318 Chemical denotes nucleozin
28 1481-1488 Disease denotes cancers MESH:D009369
29 1493-1509 Disease denotes viral infections MESH:D001102
49 3162-3165 Gene denotes RNP Gene:55599
50 3180-3183 Gene denotes RNP Gene:55599
51 2552-2607 Species denotes Middle East respiratory syndrome coronavirus (MERS-CoV) Tax:1335626
52 2623-2638 Species denotes betacoronavirus Tax:694002
53 2724-2730 Species denotes humans Tax:9606
54 2765-2782 Species denotes novel coronavirus Tax:2697049
55 2901-2905 Species denotes CoVs Tax:11118
56 2985-2989 Species denotes CoVs Tax:11118
57 3061-3065 Species denotes CoVs Tax:11118
58 3072-3080 Species denotes MERS-CoV Tax:1335626
59 3284-3287 Species denotes CoV Tax:11118
60 3393-3396 Species denotes CoV Tax:11118
61 3543-3546 Species denotes CoV Tax:11118
62 2640-2646 Species denotes β-CoVs Tax:694002
63 2673-2693 Disease denotes respiratory distress MESH:D012128
64 2784-2808 Disease denotes coronavirus disease 2019 MESH:C000657245
65 2810-2818 Disease denotes COVID-19 MESH:C000657245
66 2843-2852 Disease denotes pneumonia MESH:D011014
67 3621-3631 Disease denotes CoV N-CTDs MESH:C000657245
77 3781-3789 Species denotes MERS-CoV Tax:1335626
78 4422-4430 Species denotes MERS-CoV Tax:1335626
79 4082-4087 Chemical denotes P1–P3
80 4139-4157 Chemical denotes 5-benzyloxygramine MESH:C006736
81 4159-4161 Chemical denotes P3
82 4297-4299 Chemical denotes P3
83 4431-4436 Chemical denotes N-NTD
84 4452-4470 Chemical denotes 5-benzyloxygramine MESH:C006736
85 4731-4750 Disease denotes infectious diseases MESH:D003141
87 4804-4812 Species denotes MERS-CoV Tax:1335626
93 4862-4870 Species denotes MERS-CoV Tax:1335626
94 4930-4939 Species denotes HCoV-OC43 Tax:31631
95 5513-5517 Species denotes CoVs Tax:11118
96 5624-5627 Species denotes CoV Tax:11118
97 5151-5156 Chemical denotes N-NTD
111 6096-6098 Gene denotes β4 Gene:28898
112 6103-6105 Gene denotes β5 Gene:28884
113 5811-5819 Species denotes MERS-CoV Tax:1335626
114 7504-7507 Species denotes CoV Tax:11118
115 8174-8182 Species denotes MERS-CoV Tax:1335626
116 6513-6521 Chemical denotes hydrogen MESH:D006859
117 6536-6539 Chemical denotes N68 MESH:C495502
118 6650-6657 Chemical denotes oxygens MESH:D010100
119 6708-6716 Chemical denotes hydrogen MESH:D006859
120 6842-6846 Chemical denotes N139
121 6869-6877 Chemical denotes hydrogen MESH:D006859
122 6903-6909 Chemical denotes oxygen MESH:D010100
123 7874-7879 Chemical denotes N-NTD
131 8684-8700 Gene denotes monomers 1 and 2
132 9110-9126 Gene denotes monomers 1 and 2
133 8607-8615 Species denotes MERS-CoV Tax:1335626
134 8652-8660 Species denotes MERS-CoV Tax:1335626
135 9892-9895 Species denotes CoV Tax:11118
136 8661-8666 Chemical denotes N-NTD
137 8876-8886 Disease denotes CoV N-NTDs MESH:C000657245
163 11966-11974 Species denotes MERS-CoV Tax:1335626
164 12078-12086 Species denotes MERS-CoV Tax:1335626
165 12261-12269 Species denotes MERS-CoV Tax:1335626
166 12432-12440 Species denotes MERS-CoV Tax:1335626
167 10293-10298 Chemical denotes N-NTD
168 10665-10670 Chemical denotes N-NTD
169 10886-10891 Chemical denotes N-NTD
170 11158-11204 Chemical denotes Benzyl-2-(hydroxymethyl)-1-indolinecarboxylate
171 11206-11208 Chemical denotes P1 MESH:C480041
172 11214-11232 Chemical denotes 5-benzyloxygramine MESH:C006736
173 11234-11236 Chemical denotes P3
174 11307-11315 Chemical denotes etodolac MESH:D017308
175 11317-11319 Chemical denotes P2 MESH:C020845
176 11420-11422 Chemical denotes P3
177 11482-11487 Chemical denotes N-NTD
178 11564-11575 Chemical denotes tryptophans MESH:D014364
179 11651-11653 Chemical denotes P3
180 11845-11850 Chemical denotes N-NTD
181 11920-11922 Chemical denotes P3
182 12008-12010 Chemical denotes P3
183 12060-12062 Chemical denotes P3
184 12385-12387 Chemical denotes P3
185 11604-11612 Disease denotes rigidity MESH:D009127
186 12247-12255 Disease denotes infected MESH:D007239
187 12238-12240 CellLine denotes E6 CVCL:4582
193 12495-12503 Species denotes MERS-CoV Tax:1335626
194 12461-12463 Chemical denotes P3
195 12884-12889 Chemical denotes P1–P3
196 12940-12948 Chemical denotes Tris-HCl
197 12969-12973 Chemical denotes NaCl MESH:D012965
201 13076-13078 Gene denotes μM
202 13138-13144 Chemical denotes NAd P2
203 13160-13166 Chemical denotes NAd P3
205 13209-13217 Disease denotes toxicity MESH:D064420
208 13348-13350 Chemical denotes P3
209 13359-13389 Disease denotes MERS-CoV N Protein Aggregation MESH:C000657245
214 13450-13458 Species denotes MERS-CoV Tax:1335626
215 13741-13749 Species denotes MERS-CoV Tax:1335626
216 13554-13556 Chemical denotes P3
217 13800-13802 Chemical denotes P3
233 13865-13873 Species denotes MERS-CoV Tax:1335626
234 13924-13932 Species denotes MERS-CoV Tax:1335626
235 14588-14596 Species denotes MERS-CoV Tax:1335626
236 13814-13816 Chemical denotes P3
237 14116-14120 Chemical denotes N-P3
238 14235-14239 Chemical denotes N-P3
239 14362-14366 Chemical denotes N-P3
240 14436-14443 Chemical denotes carbons MESH:D002244
241 14629-14631 Chemical denotes P3
242 14680-14688 Chemical denotes Tris-HCl
243 14697-14701 Chemical denotes NaCl MESH:D012965
244 14890-14892 Chemical denotes P3
245 14922-14928 Chemical denotes N-NTDs
246 15145-15151 Chemical denotes N-CTDs
247 13825-13845 Disease denotes abnormal aggregation MESH:D001791
264 16676-16679 Gene denotes RNP Gene:55599
265 17805-17808 Gene denotes RNP Gene:55599
266 17765-17768 Gene denotes RNP Gene:55599
267 17607-17610 Gene denotes RNP Gene:55599
268 17058-17061 Gene denotes RNP Gene:55599
269 15970-15973 Species denotes CoV Tax:11118
270 16295-16303 Species denotes MERS-CoV Tax:1335626
271 16608-16616 Species denotes MERS-CoV Tax:1335626
272 17796-17804 Species denotes MERS-CoV Tax:1335626
273 15992-15996 Chemical denotes N-P3
274 16365-16367 Chemical denotes P3
275 16536-16538 Chemical denotes P3
276 16579-16584 Chemical denotes N-NTD
277 16708-16713 Chemical denotes N-CTD
278 15633-15637 Disease denotes fits MESH:D012640
279 17174-17191 Disease denotes N-CTD aggregation MESH:D001791
281 17867-17875 Species denotes MERS-CoV Tax:1335626
299 18503-18511 Species denotes MERS-CoV Tax:1335626
300 18995-19003 Species denotes MERS-CoV Tax:1335626
301 19171-19179 Species denotes MERS-CoV Tax:1335626
302 19230-19238 Species denotes MERS-CoV Tax:1335626
303 18013-18021 Species denotes MERS-CoV Tax:1335626
304 17931-17933 Chemical denotes P3
305 18362-18364 Chemical denotes P3
306 18457-18459 Chemical denotes P3
307 18789-18791 Chemical denotes P3
308 18799-18801 Chemical denotes P3
309 18557-18565 Disease denotes infected MESH:D007239
310 18747-18755 Disease denotes infected MESH:D007239
311 19026-19034 Disease denotes infected MESH:D007239
312 19157-19165 Disease denotes infected MESH:D007239
313 19251-19271 Disease denotes abnormal aggregation MESH:D001791
314 18183-18185 CellLine denotes E6 CVCL:4582
315 18761-18763 CellLine denotes E6 CVCL:4582
322 19666-19671 Gene denotes GAPDH Gene:2597
323 19437-19445 Species denotes MERS-CoV Tax:1335626
324 19486-19494 Species denotes MERS-CoV Tax:1335626
325 19847-19855 Species denotes MERS-CoV Tax:1335626
326 19898-19900 Chemical denotes P3
327 20032-20036 Chemical denotes DAPI MESH:C007293
330 20060-20068 Species denotes MERS-CoV Tax:1335626
331 20070-20074 Chemical denotes -NTD
352 20142-20150 Species denotes MERS-CoV Tax:1335626
353 20445-20453 Species denotes MERS-CoV Tax:1335626
354 20612-20620 Species denotes MERS-CoV Tax:1335626
355 20290-20295 Chemical denotes N-NTD
356 21131-21138 Chemical denotes benzene MESH:D001554
357 21154-21162 Chemical denotes indoline MESH:C057812
358 21174-21178 Chemical denotes D143
359 21218-21226 Chemical denotes hydrogen MESH:D006859
360 21253-21260 Chemical denotes benzene MESH:D001554
361 21328-21330 Chemical denotes P1 MESH:C480041
362 21332-21334 Chemical denotes P3
363 21434-21439 Chemical denotes N-NTD
364 21638-21640 Chemical denotes P3
365 21938-21945 Chemical denotes benzene MESH:D001554
366 21955-21958 Chemical denotes N68 MESH:C495502
367 21976-21980 Chemical denotes A109
368 22040-22059 Chemical denotes dimethylaminomethyl
369 22070-22072 Chemical denotes P3
370 22419-22421 Chemical denotes P3
371 22425-22430 Chemical denotes N-NTD
386 22783-22811 Gene denotes P1 complex (monomers 1 and 2
387 22885-22901 Gene denotes monomers 1 and 2
388 22583-22591 Species denotes MERS-CoV Tax:1335626
389 22664-22673 Species denotes HCoV-OC43 Tax:31631
390 22768-22776 Species denotes MERS-CoV Tax:1335626
391 22858-22866 Species denotes MERS-CoV Tax:1335626
392 23196-23204 Species denotes MERS-CoV Tax:1335626
393 23518-23526 Species denotes MERS-CoV Tax:1335626
394 23898-23906 Species denotes MERS-CoV Tax:1335626
395 22592-22597 Chemical denotes N-NTD
396 22674-22679 Chemical denotes N-NTD
397 22873-22875 Chemical denotes P3
398 23205-23210 Chemical denotes N-NTD
399 23886-23888 Chemical denotes P3
418 25815-25818 Gene denotes RNP Gene:55599
419 25610-25618 Species denotes MERS-CoV Tax:1335626
420 25661-25669 Species denotes MERS-CoV Tax:1335626
421 26080-26095 Species denotes β-coronaviruses Tax:694002
422 26104-26112 Species denotes MERS-CoV Tax:1335626
423 26296-26311 Species denotes β-coronaviruses Tax:694002
424 26616-26637 Species denotes mouse hepatitis virus Tax:11138
425 24500-24509 Species denotes influenza Tax:11309
426 26639-26642 Species denotes MHV Tax:11138
427 26721-26724 Species denotes MHV Tax:11138
428 24519-24528 Chemical denotes nucleozin
429 24712-24728 Chemical denotes marine macrolide
430 25770-25775 Chemical denotes N-CTD
431 26181-26186 Chemical denotes N-NTD
432 27339-27347 Chemical denotes hydrogen MESH:D006859
433 27348-27357 Chemical denotes deuterium MESH:D003903
434 25697-25727 Disease denotes abnormal N protein aggregation MESH:D001791
435 26585-26593 Disease denotes COVID-19 MESH:C000657245
437 27693-27701 Species denotes MERS-CoV Tax:1335626
446 28514-28522 Species denotes MERS-CoV Tax:1335626
447 28625-28633 Species denotes MERS-CoV Tax:1335626
448 28963-28990 Species denotes Escherichia coli BL21 (DE3) Tax:469008
449 28731-28740 Chemical denotes histidine MESH:D006639
450 29523-29529 Chemical denotes Ni–NTA
451 29599-29608 Chemical denotes imidazole MESH:C029899
452 29700-29704 Chemical denotes salt MESH:D012492
453 28806-28810 Mutation denotes N39G p.N39G
470 30048-30052 Gene denotes mL–1 Gene:23961
471 30819-30837 Gene denotes SP44XU of SPring-8
472 29849-29857 Species denotes MERS-CoV Tax:1335626
473 29911-29919 Species denotes MERS-CoV Tax:1335626
474 30260-30268 Species denotes MERS-CoV Tax:1335626
475 30357-30365 Species denotes MERS-CoV Tax:1335626
476 30432-30440 Species denotes MERS-CoV Tax:1335626
477 30560-30568 Species denotes MERS-CoV Tax:1335626
478 30763-30771 Species denotes MERS-CoV Tax:1335626
479 30133-30149 Chemical denotes ammonium sulfate MESH:D000645
480 30156-30160 Chemical denotes NaBr
481 30170-30173 Chemical denotes PEG
482 30275-30277 Chemical denotes P3
483 30353-30355 Chemical denotes P3
484 30772-30777 Chemical denotes N-NTD
485 30778-30780 Chemical denotes P3
487 31037-31046 Species denotes HCoV-OC43 Tax:31631
494 31334-31342 Species denotes MERS-CoV Tax:1335626
495 31369-31383 Chemical denotes glutaraldehyde MESH:D005976
496 31516-31524 Chemical denotes Tris-HCl
497 31587-31609 Chemical denotes sodium dodecyl sulfate MESH:D012967
498 31610-31624 Chemical denotes polyacrylamide MESH:C016679
499 31646-31649 Chemical denotes SDS MESH:D012967
504 31810-31818 Species denotes MERS-CoV Tax:1335626
505 31820-31824 Chemical denotes -NTD
506 32269-32277 Chemical denotes PLATINUM MESH:D010984
507 31778-31789 Disease denotes MERS N-NTDs MESH:D018352
511 32436-32444 Chemical denotes Tris-HCl
512 32465-32469 Chemical denotes NaCl MESH:D012965
513 32583-32593 Chemical denotes Tryptophan MESH:D014364
516 32898-32906 Chemical denotes Tris-HCl
517 32927-32931 Chemical denotes NaCl MESH:D012965
522 33380-33388 Species denotes MERS-CoV Tax:1335626
523 33366-33374 Disease denotes infected MESH:D007239
524 33641-33654 Disease denotes MERS-infected MESH:D018352
525 33352-33354 CellLine denotes E6 CVCL:4582
538 33910-33915 Gene denotes SEC-3 Gene:55763
539 33998-34006 Species denotes MERS-CoV Tax:1335626
540 34013-34021 Species denotes MERS-CoV Tax:1335626
541 35264-35272 Species denotes MERS-CoV Tax:1335626
542 35298-35306 Species denotes MERS-CoV Tax:1335626
543 35359-35367 Species denotes MERS-CoV Tax:1335626
544 35699-35707 Species denotes MERS-CoV Tax:1335626
545 35736-35744 Species denotes MERS-CoV Tax:1335626
546 34024-34026 Chemical denotes P3
547 34095-34097 Chemical denotes P3
548 34145-34153 Chemical denotes Tris-HCl
549 34174-34178 Chemical denotes NaCl MESH:D012965
551 35860-35875 Disease denotes Viral Infection MESH:D001102
560 36042-36050 Species denotes MERS-CoV Tax:1335626
561 35956-35990 Chemical denotes Dulbecco’s modified Eagle’s medium
562 35992-35996 Chemical denotes DMEM
563 36201-36226 Chemical denotes phosphate-buffered saline
564 36228-36231 Chemical denotes PBS
565 36031-36040 Disease denotes infection MESH:D007239
566 36088-36097 Disease denotes infection MESH:D007239
567 35881-35883 CellLine denotes E6 CVCL:4582
576 36445-36453 Species denotes MERS-CoV Tax:1335626
577 36620-36623 Chemical denotes PBS
578 36676-36683 Chemical denotes agarose MESH:D012685
579 36752-36755 Chemical denotes CO2 MESH:D002245
580 36805-36813 Chemical denotes formalin MESH:D005557
581 36836-36850 Chemical denotes crystal violet MESH:D005840
582 36537-36546 Disease denotes agitation MESH:D011595
583 36344-36346 CellLine denotes E6 CVCL:4582
594 37348-37353 Gene denotes GAPDH Gene:2597
595 37384-37389 Gene denotes GAPDH Gene:2597
596 37559-37564 Gene denotes GAPDH Gene:2597
597 36962-36965 Gene denotes Kit Gene:103235672
598 37137-37140 Gene denotes Kit Gene:103235672
599 37423-37431 Species denotes MERS-CoV Tax:1335626
600 37464-37472 Species denotes MERS-CoV Tax:1335626
601 37586-37594 Species denotes MERS-CoV Tax:1335626
602 36904-36912 Disease denotes infected MESH:D007239
603 36918-36920 CellLine denotes E6 CVCL:4582
615 37761-37769 Species denotes MERS-CoV Tax:1335626
616 38004-38012 Species denotes MERS-CoV Tax:1335626
617 37822-37838 Chemical denotes paraformaldehyde MESH:C003043
618 37902-37914 Chemical denotes Triton X-100 MESH:D017830
619 38171-38174 Chemical denotes PBS
620 38195-38209 Chemical denotes Alex Fluor 568
621 38327-38356 Chemical denotes 4′,6-Diamidino-2-phenylindole MESH:C007293
622 38358-38362 Chemical denotes DAPI MESH:C007293
623 38385-38388 Chemical denotes PBS
624 37746-37755 Disease denotes infection MESH:D007239
625 37663-37665 CellLine denotes E6 CVCL:4582
631 38682-38690 Species denotes MERS-CoV Tax:1335626
632 38734-38742 Species denotes MERS-CoV Tax:1335626
633 38814-38822 Species denotes MERS-CoV Tax:1335626
634 39023-39044 Species denotes mouse hepatitis virus Tax:11138
635 39018-39021 Species denotes MHV Tax:11138
637 39525-39529 Chemical denotes N-P3
639 40921-40936 Disease denotes hepatitis virus MESH:D006525

2_test

Id Subject Object Predicate Lexical cue
32105468-28968506-61929489 1512-1513 28968506 denotes 3
32105468-30957264-61929490 1741-1742 30957264 denotes 4
32105468-30913483-61929491 1743-1744 30913483 denotes 7
32105468-28283069-61929492 2029-2031 28283069 denotes 10
32105468-29268608-61929493 2549-2551 29268608 denotes 11
32105468-23075143-61929494 2731-2733 23075143 denotes 12
32105468-32402160-61929495 2934-2936 32402160 denotes 15
32105468-31953166-61929496 2937-2939 31953166 denotes 16
32105468-31099550-61929497 3069-3071 31099550 denotes 17
32105468-26691874-61929498 3383-3385 26691874 denotes 18
32105468-26868298-61929499 3386-3388 26868298 denotes 19
32105468-25105276-61929500 3533-3535 25105276 denotes 20
32105468-27344959-61929501 3536-3538 27344959 denotes 21
32105468-24564608-61929502 3734-3736 24564608 denotes 22
32105468-30644840-61929503 3737-3739 30644840 denotes 23
32105468-16338414-61929504 5518-5520 16338414 denotes 25
32105468-23389845-61929505 7160-7162 23389845 denotes 26
32105468-26894667-61929506 7521-7523 26894667 denotes 27
32105468-19782089-61929507 7524-7526 19782089 denotes 30
32105468-26894667-61929508 8203-8205 26894667 denotes 27
32105468-19244385-61929509 10943-10945 19244385 denotes 31
32105468-30644840-61929510 15515-15517 30644840 denotes 23
32105468-28044277-61929511 15985-15987 28044277 denotes 33
32105468-28044277-61929512 16786-16788 28044277 denotes 33
32105468-16775348-61929513 17127-17129 16775348 denotes 28
32105468-17379242-61929514 17130-17132 17379242 denotes 34
32105468-31141413-61929515 24347-24349 31141413 denotes 35
32105468-28300404-61929516 24350-24352 28300404 denotes 37
32105468-27820802-61929517 24488-24490 27820802 denotes 38
32105468-20512121-61929518 24683-24685 20512121 denotes 40
32105468-15797212-61929519 24906-24908 15797212 denotes 41
32105468-7876075-61929520 24909-24911 7876075 denotes 43
32105468-29308120-61929521 25064-25066 29308120 denotes 44
32105468-16775348-61929522 26315-26317 16775348 denotes 28
32105468-16503614-61929523 27370-27372 16503614 denotes 45
32105468-26249685-61929524 28590-28592 26249685 denotes 46
32105468-26249685-61929525 29908-29910 26249685 denotes 46
32105468-12393927-61929526 31028-31030 12393927 denotes 47
32105468-15572765-61929527 31138-31140 15572765 denotes 48
32105468-19244385-61929528 32278-32280 19244385 denotes 31
32105468-25484842-61929529 35114-35116 25484842 denotes 50
32105468-26894667-61929530 35291-35293 26894667 denotes 27
32105468-30644840-61929531 35392-35394 30644840 denotes 23
32105468-22026976-61929532 37420-37422 22026976 denotes 53
32105468-26432410-61929533 37509-37511 26432410 denotes 54