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PMC:7091888 / 9890-13134 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
329 52-56 Gene denotes ACE2 Gene:59272
330 192-196 Gene denotes ACE2 Gene:59272
331 198-203 Gene denotes hACE2 Gene:59272
332 232-237 Gene denotes hDPP4 Gene:1803
333 298-308 Gene denotes hACE2/293T Gene:59272
334 331-336 Gene denotes hACE2 Gene:59272
335 360-365 Gene denotes hDPP4 Gene:1803
336 416-426 Gene denotes hDPP4/293T Gene:1803
337 449-454 Gene denotes hDPP4 Gene:1803
338 476-481 Gene denotes hACE2 Gene:59272
339 737-742 Gene denotes hACE2 Gene:59272
340 755-765 Gene denotes hACE2/293T Gene:59272
341 826-831 Gene denotes hACE2 Gene:59272
342 960-965 Gene denotes hACE2 Gene:59272
343 1105-1110 Gene denotes hACE2 Gene:59272
344 1505-1510 Gene denotes hACE2 Gene:59272
345 1523-1533 Gene denotes hACE2/293T Gene:59272
346 1722-1727 Gene denotes hACE2 Gene:59272
347 1757-1762 Gene denotes hACE2 Gene:59272
348 1890-1895 Gene denotes hACE2 Gene:59272
349 1905-1910 Gene denotes hACE2 Gene:59272
350 1955-1960 Gene denotes hACE2 Gene:59272
351 2168-2173 Gene denotes hACE2 Gene:59272
352 2557-2562 Gene denotes hACE2 Gene:59272
353 2623-2628 Gene denotes hDPP4 Gene:1803
354 2736-2741 Gene denotes hACE2 Gene:59272
355 2792-2797 Gene denotes hACE2 Gene:59272
356 20-30 Species denotes SARS-CoV-2 Tax:2697049
357 46-51 Species denotes human Tax:9606
358 186-191 Species denotes human Tax:9606
359 695-705 Species denotes SARS-CoV-2 Tax:2697049
360 785-795 Species denotes SARS-CoV-2 Tax:2697049
361 930-938 Species denotes SARS-CoV Tax:694009
362 1463-1473 Species denotes SARS-CoV-2 Tax:2697049
363 1541-1551 Species denotes SARS-CoV-2 Tax:2697049
364 1576-1584 Species denotes SARS-CoV Tax:694009
365 2134-2144 Species denotes SARS-CoV-2 Tax:2697049
366 2205-2215 Species denotes SARS-CoV-2 Tax:2697049
367 2291-2299 Species denotes SARS-CoV Tax:694009
368 2308-2316 Species denotes MERS-CoV Tax:1335626
369 2508-2518 Species denotes SARS-CoV-2 Tax:2697049
370 2534-2542 Species denotes SARS-CoV Tax:694009
371 2702-2712 Species denotes SARS-CoV-2 Tax:2697049
372 2774-2784 Species denotes SARS-CoV-2 Tax:2697049
373 1220-1225 Species denotes Human Tax:9606
374 1694-1699 Species denotes human Tax:9606
375 2239-2244 Gene denotes hDPP4 Gene:1803
376 1671-1675 Chemical denotes FITC MESH:D016650
377 1786-1801 Chemical denotes Alexa-Fluor 647 MESH:C569686
378 1998-2027 Chemical denotes 4’,6-diamidino-2-phenylindole MESH:C007293
379 2029-2033 Chemical denotes DAPI MESH:C007293
380 151-155 CellLine denotes 293T CVCL:0063
381 292-296 CellLine denotes 293T CVCL:0063
382 410-414 CellLine denotes 293T CVCL:0063
383 832-836 CellLine denotes 293T CVCL:0063
384 888-892 CellLine denotes 293T CVCL:0063
385 966-970 CellLine denotes 293T CVCL:0063
386 1024-1028 CellLine denotes 293T CVCL:0063
387 1111-1115 CellLine denotes 293T CVCL:0063
388 1177-1181 CellLine denotes 293T CVCL:0063
389 1961-1965 CellLine denotes 293T CVCL:0063
390 2742-2746 CellLine denotes 293T CVCL:0063
391 2798-2802 CellLine denotes 293T CVCL:0063

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T61 126-130 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T62 156-161 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T63 310-315 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T64 428-433 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T65 512-517 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T66 721-725 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T67 773-777 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T68 800-807 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T69 837-842 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T70 893-898 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T71 943-950 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T72 971-976 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T73 1029-1034 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T74 1081-1088 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T75 1116-1121 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T76 1182-1187 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T77 1226-1229 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872
T78 1260-1267 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T79 1278-1283 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T80 1315-1320 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T81 1489-1493 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T82 1534-1539 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T83 1556-1563 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T84 1589-1596 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T85 1700-1703 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872
T86 1704-1712 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T87 1763-1771 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T88 1820-1828 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T89 1866-1873 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T90 1966-1971 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T91 2149-2156 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T92 2321-2329 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T93 2384-2391 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T94 2563-2570 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T95 2629-2636 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T96 2717-2724 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T97 2747-2752 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T98 2803-2808 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T99 2902-2909 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T100 2920-2925 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T101 2957-2962 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 2042-2047 Body_part denotes Scale http://purl.obolibrary.org/obo/UBERON_0002542

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T178 20-28 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T179 20-24 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T180 695-703 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T181 695-699 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T182 785-793 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T183 785-789 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T184 930-938 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T185 930-934 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T186 1463-1471 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T187 1463-1467 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T188 1541-1549 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T189 1541-1545 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T190 1576-1584 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T191 1576-1580 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T192 2134-2142 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T193 2134-2138 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T194 2205-2213 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T195 2205-2209 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T196 2291-2299 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T197 2291-2295 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T198 2508-2516 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T199 2508-2512 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T200 2534-2542 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T201 2534-2538 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T202 2702-2710 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T203 2702-2706 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T204 2774-2782 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T205 2774-2778 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T140 46-51 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T141 67-68 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T142 126-136 http://purl.obolibrary.org/obo/CLO_0000031 denotes cell lines
T143 151-155 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T144 151-155 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T145 151-155 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T146 156-161 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T147 186-191 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T148 292-296 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T149 292-296 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T150 292-296 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T151 304-308 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T152 304-308 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T153 304-308 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T154 310-315 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T155 410-414 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T156 410-414 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T157 410-414 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T158 422-426 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T159 422-426 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T160 422-426 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T161 428-433 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T162 512-517 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T163 663-664 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T164 721-725 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T165 761-765 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T166 761-765 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T167 761-765 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T168 773-783 http://purl.obolibrary.org/obo/CLO_0000031 denotes cell lines
T169 832-836 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T170 832-836 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T171 832-836 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T172 837-842 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T173 844-845 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T174 888-892 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T175 888-892 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T176 888-892 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T177 893-898 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T178 966-970 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T179 966-970 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T180 966-970 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T181 971-976 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T182 978-979 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T183 1024-1028 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T184 1024-1028 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T185 1024-1028 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T186 1029-1034 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T187 1111-1115 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T188 1111-1115 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T189 1111-1115 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T190 1116-1121 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T191 1123-1124 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T192 1177-1181 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T193 1177-1181 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T194 1177-1181 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T195 1182-1187 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T196 1220-1229 http://purl.obolibrary.org/obo/CLO_0053695 denotes Human IgG
T197 1230-1232 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T198 1239-1241 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T199 1278-1283 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T200 1315-1320 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T201 1363-1364 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T202 1489-1493 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T203 1529-1533 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T204 1529-1533 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T205 1529-1533 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T206 1534-1539 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T207 1635-1636 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T208 1676-1683 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T209 1684-1688 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T210 1694-1703 http://purl.obolibrary.org/obo/CLO_0053695 denotes human IgG
T211 1745-1746 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T212 1747-1751 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T213 1802-1809 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T214 1815-1819 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T215 1830-1837 http://purl.obolibrary.org/obo/CLO_0008776 denotes red) (1
T216 1830-1837 http://purl.obolibrary.org/obo/CLO_0008777 denotes red) (1
T217 1844-1846 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T218 1961-1965 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T219 1961-1965 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T220 1961-1965 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T221 1966-1971 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T222 2363-2369 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T223 2742-2746 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T224 2742-2746 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T225 2742-2746 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T226 2747-2752 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T227 2798-2802 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T228 2798-2802 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T229 2798-2802 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T230 2803-2808 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T231 2920-2925 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T232 2957-2962 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T47 800-807 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T48 943-950 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T49 1081-1088 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T50 1260-1267 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T51 1556-1563 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T52 1589-1596 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T53 1671-1675 Chemical denotes FITC http://purl.obolibrary.org/obo/CHEBI_37926
T54 1792-1797 Chemical denotes Fluor http://purl.obolibrary.org/obo/CHEBI_24061
T55 1866-1873 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T56 2015-2027 Chemical denotes phenylindole http://purl.obolibrary.org/obo/CHEBI_48559
T57 2029-2033 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T58 2149-2156 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T59 2321-2329 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T60 2384-2391 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T61 2563-2570 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T62 2629-2636 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T63 2717-2724 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T64 2902-2909 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T51 0-496 Sentence denotes Fig. 2 Detection of SARS-CoV-2 RBD binding to human ACE2 receptor. a Flow cytometry analysis of receptor expression in stable cell lines. (left panel) 293T cells alone expressed neither human ACE2 (hACE2) receptor (orange line) nor hDPP4 receptor (cyan line); (middle panel) hACE2-expressing 293T (hACE2/293T) cells expressed only hACE2 (orange line), but not hDPP4 (cyan line); (right panel) hDPP4-expressing 293T (hDPP4/293T) cells expressed only hDPP4 (cyan line), but not hACE2 (orange line).
T52 497-554 Sentence denotes Mock-incubated cells (gray shading) were used as control.
T53 555-784 Sentence denotes Representative images and median fluorescence intensity (MFI) ± standard error (s.e.m.) were shown (n = 4). b, c Flow cytometry analysis of SARS-CoV-2 RBD binding to cell-associated hACE2 receptor in hACE2/293T stable cell lines.
T54 785-929 Sentence denotes SARS-CoV-2 RBD protein bound strongly to hACE2/293T cells (b (left panel, red line)), but not to hDPP4/293T cells (c (left panel, violet line)).
T55 930-1067 Sentence denotes SARS-CoV RBD protein bound to hACE2/293T cells (b (middle panel, red line)), but not to hDPP4/293T cells (c (middle panel, violet line)).
T56 1068-1219 Sentence denotes MERS-CoV RBD protein did not bind to hACE2/293T cells (b (right panel, red line)), but rather bound to hDPP4/293T cells (c (right panel, violet line)).
T57 1220-1369 Sentence denotes Human IgG Fc (hIgG-Fc, hereinafter hFc) protein-incubated cells (blue line) and mock-incubated cells (gray shading) were included as controls (b, c).
T58 1370-1540 Sentence denotes Representative images and MFI ± s.e.m. were shown (n = 4). d Immunofluorescence detection of SARS-CoV-2 RBD binding to cell-associated hACE2 receptor in hACE2/293T cells.
T59 1541-1843 Sentence denotes SARS-CoV-2 RBD protein (green) and SARS-CoV RBD protein (green), each of which was fused with a C-terminal hFc, were stained with FITC-labeled goat anti-human IgG antibody (1:500). hACE2 was stained with a goat-anti-hACE2 antibody (5 μg/ml) and Alexa-Fluor 647-labeled anti-goat antibody (red) (1:200).
T60 1844-1972 Sentence denotes Fc-fused MERS-CoV RBD protein did not bind to hACE2, so only hACE2 (red), but not RBD (green), was detected in hACE2/293T cells.
T61 1973-2041 Sentence denotes Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).
T62 2042-2052 Sentence denotes Scale bar:
T63 2053-2059 Sentence denotes 10 μm.
T64 2060-2200 Sentence denotes Representative images are shown. e Detection of dose-dependent binding of SARS-CoV-2 RBD protein to soluble hACE2 (sACE2) receptor by ELISA.
T65 2201-2370 Sentence denotes The SARS-CoV-2 RBD binding to soluble hDPP4 (sDPP4) receptor (f), and the binding of both SARS-CoV RBD and MERS-CoV RBD proteins to sACE2 (e), or sDPP4 (f), were tested.
T66 2371-2392 Sentence denotes Control: hFc protein.
T67 2393-2442 Sentence denotes Data are presented as mean A450 ± s.e.m. (n = 4).
T68 2443-2762 Sentence denotes 50% effective dose (EC50) was calculated for the binding between SARS-CoV-2 RBD (black) or SARS-CoV RBD (red) and hACE2 protein (e, sACE2), or the binding between MERS-CoV RBD and hDPP4 protein (sDPP4, green) (f). g–i Flow cytometry analysis of inhibition of SARS-CoV-2 RBD protein binding to hACE2/293T cells by sACE2.
T69 2763-3011 Sentence denotes Binding of SARS-CoV-2 RBD to hACE2/293T cells (g, h, green line) was blocked by sACE2 (g, black line), but not by sDPP4 (h, red line). hFc protein-incubated cells (blue line) and mock-incubated cells (gray shading) were included as controls (g, h).
T70 3012-3145 Sentence denotes Representative images are shown. i The blocking ability of sACE2 or sDPP4, as described above, was expressed as MFI ± s.e.m. (n = 4).
T71 3146-3184 Sentence denotes Low MFI correlates with high blockage.
T72 3185-3244 Sentence denotes Experiments were repeated twice and yielded similar results