PMC:7091888 / 27411-28364 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1099","span":{"begin":519,"end":524},"obj":"Gene"},{"id":"1100","span":{"begin":529,"end":534},"obj":"Gene"},{"id":"1101","span":{"begin":45,"end":55},"obj":"Species"},{"id":"1102","span":{"begin":120,"end":128},"obj":"Species"},{"id":"1103","span":{"begin":133,"end":141},"obj":"Species"},{"id":"1104","span":{"begin":239,"end":249},"obj":"Species"},{"id":"1105","span":{"begin":251,"end":259},"obj":"Species"}],"attributes":[{"id":"A1099","pred":"tao:has_database_id","subj":"1099","obj":"Gene:59272"},{"id":"A1100","pred":"tao:has_database_id","subj":"1100","obj":"Gene:1803"},{"id":"A1101","pred":"tao:has_database_id","subj":"1101","obj":"Tax:2697049"},{"id":"A1102","pred":"tao:has_database_id","subj":"1102","obj":"Tax:694009"},{"id":"A1103","pred":"tao:has_database_id","subj":"1103","obj":"Tax:1335626"},{"id":"A1104","pred":"tao:has_database_id","subj":"1104","obj":"Tax:2697049"},{"id":"A1105","pred":"tao:has_database_id","subj":"1105","obj":"Tax:694009"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T222","span":{"begin":60,"end":67},"obj":"Body_part"},{"id":"T223","span":{"begin":165,"end":172},"obj":"Body_part"},{"id":"T224","span":{"begin":334,"end":338},"obj":"Body_part"},{"id":"T225","span":{"begin":399,"end":406},"obj":"Body_part"},{"id":"T226","span":{"begin":492,"end":499},"obj":"Body_part"},{"id":"T227","span":{"begin":549,"end":557},"obj":"Body_part"},{"id":"T228","span":{"begin":653,"end":656},"obj":"Body_part"},{"id":"T229","span":{"begin":657,"end":665},"obj":"Body_part"}],"attributes":[{"id":"A222","pred":"fma_id","subj":"T222","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A223","pred":"fma_id","subj":"T223","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A224","pred":"fma_id","subj":"T224","obj":"http://purl.org/sig/ont/fma/fma62100"},{"id":"A225","pred":"fma_id","subj":"T225","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A226","pred":"fma_id","subj":"T226","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A227","pred":"fma_id","subj":"T227","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A228","pred":"fma_id","subj":"T228","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A229","pred":"fma_id","subj":"T229","obj":"http://purl.org/sig/ont/fma/fma62871"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T4","span":{"begin":334,"end":338},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T474","span":{"begin":45,"end":53},"obj":"Disease"},{"id":"T475","span":{"begin":45,"end":49},"obj":"Disease"},{"id":"T476","span":{"begin":120,"end":128},"obj":"Disease"},{"id":"T477","span":{"begin":120,"end":124},"obj":"Disease"},{"id":"T478","span":{"begin":239,"end":247},"obj":"Disease"},{"id":"T479","span":{"begin":239,"end":243},"obj":"Disease"},{"id":"T480","span":{"begin":251,"end":259},"obj":"Disease"},{"id":"T481","span":{"begin":251,"end":255},"obj":"Disease"}],"attributes":[{"id":"A474","pred":"mondo_id","subj":"T474","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A475","pred":"mondo_id","subj":"T475","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A476","pred":"mondo_id","subj":"T476","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A477","pred":"mondo_id","subj":"T477","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A478","pred":"mondo_id","subj":"T478","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A479","pred":"mondo_id","subj":"T479","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A480","pred":"mondo_id","subj":"T480","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A481","pred":"mondo_id","subj":"T481","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T480","span":{"begin":300,"end":304},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T481","span":{"begin":325,"end":328},"obj":"http://purl.obolibrary.org/obo/UBERON_0001013"},{"id":"T482","span":{"begin":544,"end":548},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T483","span":{"begin":570,"end":573},"obj":"http://purl.obolibrary.org/obo/CLO_0008693"},{"id":"T484","span":{"begin":570,"end":573},"obj":"http://purl.obolibrary.org/obo/CLO_0008770"},{"id":"T485","span":{"begin":648,"end":652},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T486","span":{"begin":818,"end":820},"obj":"http://purl.obolibrary.org/obo/CLO_0009141"},{"id":"T487","span":{"begin":818,"end":820},"obj":"http://purl.obolibrary.org/obo/CLO_0050980"},{"id":"T488","span":{"begin":829,"end":831},"obj":"http://purl.obolibrary.org/obo/CLO_0007815"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T150","span":{"begin":60,"end":67},"obj":"Chemical"},{"id":"T151","span":{"begin":165,"end":172},"obj":"Chemical"},{"id":"T152","span":{"begin":399,"end":406},"obj":"Chemical"},{"id":"T153","span":{"begin":492,"end":499},"obj":"Chemical"},{"id":"T154","span":{"begin":848,"end":853},"obj":"Chemical"}],"attributes":[{"id":"A150","pred":"chebi_id","subj":"T150","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A151","pred":"chebi_id","subj":"T151","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A152","pred":"chebi_id","subj":"T152","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A153","pred":"chebi_id","subj":"T153","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A154","pred":"chebi_id","subj":"T154","obj":"http://purl.obolibrary.org/obo/CHEBI_26836"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T160","span":{"begin":0,"end":196},"obj":"Sentence"},{"id":"T161","span":{"begin":197,"end":364},"obj":"Sentence"},{"id":"T162","span":{"begin":365,"end":462},"obj":"Sentence"},{"id":"T163","span":{"begin":463,"end":719},"obj":"Sentence"},{"id":"T164","span":{"begin":720,"end":860},"obj":"Sentence"},{"id":"T165","span":{"begin":861,"end":953},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}

{"project":"2_test","denotations":[{"id":"32203189-23824801-19774545","span":{"begin":114,"end":116},"obj":"23824801"},{"id":"32203189-24355931-19774546","span":{"begin":117,"end":119},"obj":"24355931"}],"text":"ELISA was performed to detect the binding of SARS-CoV-2 RBD protein to sACE2 receptor, as previously described.27,32,34 SARS-CoV and MERS-CoV RBDs, as well as sDPP4 protein, were used as controls. Briefly, ELISA plates were precoated with SARS-CoV-2, SARS-CoV, or MERS-CoV RBD (1 μg/ml) overnight at 4 °C and blocked with 2% fat-free milk in PBST for 2 h at 37 °C. Serially diluted sACE2, or sDPP4, protein was added to the plates and incubated for 2 h at 37 °C. After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C. The reaction was visualized by addition of substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma, St. Louis, MO) and stopped by H2SO4 (1 N). The absorbance at 450 nm (A450) was measured by an ELISA plate reader (Tecan, San Jose, CA)."}