PMC:7091888 / 24514-26543
Annnotations
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
1005 | 113-118 | Gene | denotes | hACE2 | Gene:59272 |
1006 | 160-165 | Gene | denotes | hACE2 | Gene:59272 |
1007 | 167-177 | Gene | denotes | hACE2/293T | Gene:59272 |
1008 | 269-279 | Gene | denotes | hDPP4/293T | Gene:1803 |
1009 | 703-707 | Gene | denotes | ACE2 | Gene:59272 |
1010 | 939-943 | Gene | denotes | DPP4 | Gene:1803 |
1011 | 87-97 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
1012 | 185-193 | Species | denotes | SARS-CoV | Tax:694009 |
1013 | 198-206 | Species | denotes | MERS-CoV | Tax:1335626 |
1014 | 358-368 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
1015 | 370-378 | Species | denotes | SARS-CoV | Tax:694009 |
1016 | 383-391 | Species | denotes | MERS-CoV | Tax:1335626 |
1017 | 697-702 | Species | denotes | human | Tax:9606 |
1018 | 787-797 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
1019 | 799-807 | Species | denotes | SARS-CoV | Tax:694009 |
1020 | 812-820 | Species | denotes | MERS-CoV | Tax:1335626 |
1021 | 933-938 | Species | denotes | human | Tax:9606 |
1022 | 525-530 | Species | denotes | human | Tax:9606 |
1023 | 502-506 | Chemical | denotes | FITC | MESH:D016650 |
1024 | 945-950 | Chemical | denotes | sDPP4 | |
1025 | 131-135 | CellLine | denotes | 293T | CVCL:0063 |
1026 | 224-228 | CellLine | denotes | 293T | CVCL:0063 |
1027 | 751-753 | CellLine | denotes | MN | CVCL:U508 |
1035 | 1022-1027 | Gene | denotes | hACE2 | Gene:59272 |
1036 | 1050-1060 | Gene | denotes | hACE2/293T | Gene:59272 |
1037 | 1065-1070 | Gene | denotes | hDPP4 | Gene:1803 |
1038 | 1093-1103 | Gene | denotes | hDPP4/293T | Gene:1803 |
1039 | 1240-1245 | Gene | denotes | hACE2 | Gene:59272 |
1040 | 1250-1255 | Gene | denotes | hDPP4 | Gene:1803 |
1041 | 1339-1343 | Chemical | denotes | FITC | MESH:D016650 |
1054 | 1514-1519 | Gene | denotes | hACE2 | Gene:59272 |
1055 | 1528-1532 | Gene | denotes | ACE2 | Gene:59272 |
1056 | 1632-1637 | Gene | denotes | hACE2 | Gene:59272 |
1057 | 1495-1505 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
1058 | 1732-1742 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
1059 | 1813-1821 | Species | denotes | SARS-CoV | Tax:694009 |
1060 | 1826-1834 | Species | denotes | MERS-CoV | Tax:1335626 |
1061 | 1913-1918 | Species | denotes | human | Tax:9606 |
1062 | 1665-1682 | Chemical | denotes | calcium phosphate | MESH:C020243 |
1063 | 1887-1891 | Chemical | denotes | FITC | MESH:D016650 |
1064 | 1578-1582 | CellLine | denotes | 293T | CVCL:0063 |
1065 | 1599-1603 | CellLine | denotes | 293T | CVCL:0063 |
LitCovid-PD-FMA-UBERON
Id | Subject | Object | Predicate | Lexical cue | fma_id |
---|---|---|---|---|---|
T196 | 102-109 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
T197 | 136-141 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T198 | 229-234 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T199 | 314-319 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T200 | 531-534 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
T201 | 535-543 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T202 | 897-904 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
T203 | 1028-1035 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
T204 | 1071-1078 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
T205 | 1112-1116 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
T206 | 1201-1206 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T207 | 1270-1278 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T208 | 1362-1365 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
T209 | 1366-1374 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
T210 | 1583-1588 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T211 | 1604-1609 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
T212 | 1747-1754 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
T213 | 1919-1922 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
T214 | 1923-1931 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
LitCovid-PD-MONDO
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T448 | 87-95 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T449 | 87-91 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T450 | 185-193 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T451 | 185-189 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T452 | 358-366 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T453 | 358-362 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T454 | 370-378 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T455 | 370-374 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T456 | 787-795 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T457 | 787-791 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T458 | 799-807 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T459 | 799-803 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T460 | 1495-1503 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T461 | 1495-1499 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T462 | 1732-1740 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T463 | 1732-1736 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
T464 | 1813-1821 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
T465 | 1813-1817 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
LitCovid-PD-CLO
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T413 | 131-135 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T414 | 131-135 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T415 | 131-135 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T416 | 136-141 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T417 | 173-177 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T418 | 173-177 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T419 | 173-177 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T420 | 224-228 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T421 | 224-228 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T422 | 224-228 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T423 | 229-234 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T424 | 275-279 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T425 | 275-279 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T426 | 275-279 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T427 | 314-319 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T428 | 403-404 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
T429 | 507-514 | http://purl.obolibrary.org/obo/CLO_0007225 | denotes | labeled |
T430 | 515-519 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T431 | 525-534 | http://purl.obolibrary.org/obo/CLO_0053695 | denotes | human IgG |
T432 | 697-702 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
T433 | 725-728 | http://purl.obolibrary.org/obo/CLO_0008693 | denotes | R&D |
T434 | 725-728 | http://purl.obolibrary.org/obo/CLO_0008770 | denotes | R&D |
T435 | 894-896 | http://purl.obolibrary.org/obo/CLO_0052676 | denotes | Fc |
T436 | 933-938 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
T437 | 961-964 | http://purl.obolibrary.org/obo/CLO_0008693 | denotes | R&D |
T438 | 961-964 | http://purl.obolibrary.org/obo/CLO_0008770 | denotes | R&D |
T439 | 1056-1060 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T440 | 1056-1060 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T441 | 1056-1060 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T442 | 1099-1103 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T443 | 1099-1103 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T444 | 1099-1103 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T445 | 1112-1122 | http://purl.obolibrary.org/obo/CLO_0000031 | denotes | cell lines |
T446 | 1201-1206 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T447 | 1265-1269 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T448 | 1291-1294 | http://purl.obolibrary.org/obo/CLO_0008693 | denotes | R&D |
T449 | 1291-1294 | http://purl.obolibrary.org/obo/CLO_0008770 | denotes | R&D |
T450 | 1344-1351 | http://purl.obolibrary.org/obo/CLO_0007225 | denotes | labeled |
T451 | 1357-1361 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T452 | 1416-1420 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
T453 | 1524-1527 | http://purl.obolibrary.org/obo/NCBITaxon_9397 | denotes | bat |
T454 | 1578-1582 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T455 | 1578-1582 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T456 | 1578-1582 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T457 | 1583-1588 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T458 | 1599-1603 | http://purl.obolibrary.org/obo/CLO_0050894 | denotes | 293T |
T459 | 1599-1603 | http://purl.obolibrary.org/obo/CLO_0051650 | denotes | 293T |
T460 | 1599-1603 | http://purl.obolibrary.org/obo/CLO_0052052 | denotes | 293T |
T461 | 1604-1609 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T462 | 1695-1697 | http://purl.obolibrary.org/obo/CLO_0001382 | denotes | 48 |
T463 | 1903-1907 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
T464 | 1913-1922 | http://purl.obolibrary.org/obo/CLO_0053695 | denotes | human IgG |
LitCovid-PD-CHEBI
Id | Subject | Object | Predicate | Lexical cue | chebi_id |
---|---|---|---|---|---|
T124 | 102-109 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
T125 | 502-506 | Chemical | denotes | FITC | http://purl.obolibrary.org/obo/CHEBI_37926 |
T126 | 751-753 | Chemical | denotes | MN | http://purl.obolibrary.org/obo/CHEBI_141442 |
T127 | 897-904 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
T128 | 1028-1035 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
T129 | 1071-1078 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
T130 | 1339-1343 | Chemical | denotes | FITC | http://purl.obolibrary.org/obo/CHEBI_37926 |
T131 | 1401-1403 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
T134 | 1665-1682 | Chemical | denotes | calcium phosphate | http://purl.obolibrary.org/obo/CHEBI_77635|http://purl.obolibrary.org/obo/CHEBI_9679 |
T136 | 1665-1672 | Chemical | denotes | calcium | http://purl.obolibrary.org/obo/CHEBI_22984|http://purl.obolibrary.org/obo/CHEBI_29320 |
T138 | 1673-1682 | Chemical | denotes | phosphate | http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474 |
T142 | 1747-1754 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
T143 | 1887-1891 | Chemical | denotes | FITC | http://purl.obolibrary.org/obo/CHEBI_37926 |
T144 | 1971-1978 | Chemical | denotes | mixture | http://purl.obolibrary.org/obo/CHEBI_60004 |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T143 | 0-23 | Sentence | denotes | Flow cytometry analysis |
T144 | 24-304 | Sentence | denotes | Flow cytometry analysis was performed to detect the binding of SARS-CoV-2 RBD protein to hACE2 receptor in 293T cells stably expressing hACE2 (hACE2/293T).26,32 SARS-CoV and MERS-CoV RBDs, as well as 293T cells stably expressing hDPP4 receptor (hDPP4/293T), were used as controls. |
T145 | 305-620 | Sentence | denotes | Briefly, cells were incubated with respective RBD of SARS-CoV-2, SARS-CoV, or MERS-CoV containing a C-terminal hFc at 20 μg/ml for 30 min at room temperature, which was followed by incubation with FITC-labeled goat anti-human IgG antibody (1:500; Thermo Fisher Scientific) for 30 min and analyzed by flow cytometry. |
T146 | 621-910 | Sentence | denotes | The blockage of RBD-receptor binding was performed by incubation of soluble human ACE2 (sACE2; 5 μg/ml; R&D Systems, Minneapolis, MN) receptor with respective RBD of SARS-CoV-2, SARS-CoV, or MERS-CoV (20 μg/ml), followed by the same procedure as that described above. hIgG-Fc protein (hFc: |
T147 | 911-1008 | Sentence | denotes | 20 μg/ml), or soluble human DPP4 (sDPP4; 5 μg/ml; R&D Systems) receptor, was included as control. |
T148 | 1009-1421 | Sentence | denotes | Detection of hACE2 protein expression in hACE2/293T, or hDPP4 protein expression in hDPP4/293T, stable cell lines was performed by flow cytometry analysis, as described above, except that the cells were sequentially incubated with hACE2- or hDPP4-specific goat antibody (0.5 μg/ml; R&D Systems) at room temperature for 20 min and FITC-labeled anti-goat IgG antibody (1:200; Abcam, Cambridge, MA) for 1 h at 4 °C. |
T149 | 1422-1589 | Sentence | denotes | Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. |
T150 | 1590-1812 | Sentence | denotes | Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. |
T151 | 1813-1866 | Sentence | denotes | SARS-CoV and MERS-CoV RBDs were included as controls. |
T152 | 1867-2029 | Sentence | denotes | After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above. |
2_test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
32203189-24719424-19774542 | 179-181 | 24719424 | denotes | 26 |
32203189-23824801-19774543 | 182-184 | 23824801 | denotes | 32 |