PMC:7091888 / 23720-24512
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 972 | 375-383 | Species | denotes | SARS-CoV | Tax:694009 |
| 973 | 555-566 | Species | denotes | horseradish | Tax:3704 |
| 974 | 418-423 | Species | denotes | mouse | Tax:10090 |
| 975 | 477-482 | Species | denotes | mouse | Tax:10090 |
| 976 | 605-610 | Species | denotes | mouse | Tax:10090 |
| 977 | 43-46 | Chemical | denotes | SDS | MESH:D012967 |
| 978 | 139-143 | Chemical | denotes | Tris | |
| 979 | 144-151 | Chemical | denotes | glycine | MESH:D005998 |
| 980 | 152-155 | Chemical | denotes | SDS | MESH:D012967 |
| 981 | 178-202 | Chemical | denotes | Coomassie brilliant blue | MESH:C004692 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T189 | 17-25 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T190 | 108-116 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T191 | 144-151 | Body_part | denotes | glycine | http://purl.org/sig/ont/fma/fma82753 |
| T192 | 287-291 | Body_part | denotes | milk | http://purl.org/sig/ont/fma/fma62100 |
| T193 | 408-416 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T194 | 467-475 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T195 | 611-614 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T3 | 287-291 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T446 | 375-383 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T447 | 375-379 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T406 | 236-245 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T407 | 278-281 | http://purl.obolibrary.org/obo/UBERON_0001013 | denotes | fat |
| T408 | 418-423 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T409 | 477-482 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T410 | 514-518 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T411 | 595-599 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T412 | 605-610 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T110 | 17-25 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T111 | 43-46 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T112 | 108-116 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T113 | 139-143 | Chemical | denotes | Tris | http://purl.obolibrary.org/obo/CHEBI_9754 |
| T114 | 144-151 | Chemical | denotes | glycine | http://purl.obolibrary.org/obo/CHEBI_15428|http://purl.obolibrary.org/obo/CHEBI_29947|http://purl.obolibrary.org/obo/CHEBI_57305 |
| T117 | 152-155 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T118 | 221-235 | Chemical | denotes | nitrocellulose | http://purl.obolibrary.org/obo/CHEBI_53325 |
| T119 | 660-662 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
| T122 | 744-752 | Chemical | denotes | reagents | http://purl.obolibrary.org/obo/CHEBI_33893 |
| T123 | 777-779 | Chemical | denotes | GE | http://purl.obolibrary.org/obo/CHEBI_73801 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T3 | 555-583 | http://purl.obolibrary.org/obo/GO_0004601 | denotes | horseradish peroxidase (HRP) |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T140 | 0-246 | Sentence | denotes | The purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. |
| T141 | 247-519 | Sentence | denotes | The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. |
| T142 | 520-792 | Sentence | denotes | The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare). |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 32203189-19683779-19774539 | 96-98 | 19683779 | denotes | 29 |
| 32203189-20374001-19774540 | 439-441 | 20374001 | denotes | 30 |
| 32203189-27750111-19774541 | 498-500 | 27750111 | denotes | 31 |