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LitCovid-PubTator

LitCovid-PD-FMA-UBERON

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T105","span":{"begin":161,"end":166},"obj":"Body_part"},{"id":"T106","span":{"begin":252,"end":257},"obj":"Body_part"},{"id":"T107","span":{"begin":474,"end":479},"obj":"Body_part"},{"id":"T108","span":{"begin":707,"end":712},"obj":"Body_part"},{"id":"T109","span":{"begin":798,"end":803},"obj":"Body_part"},{"id":"T110","span":{"begin":1021,"end":1026},"obj":"Body_part"},{"id":"T111","span":{"begin":1115,"end":1120},"obj":"Body_part"}],"attributes":[{"id":"A105","pred":"fma_id","subj":"T105","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A106","pred":"fma_id","subj":"T106","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A107","pred":"fma_id","subj":"T107","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A108","pred":"fma_id","subj":"T108","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A109","pred":"fma_id","subj":"T109","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A110","pred":"fma_id","subj":"T110","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A111","pred":"fma_id","subj":"T111","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"To assess the effect of GA on non-enveloped virus, we also tested its antiviral activity on adenovirus, which is internalized by endocytosis. Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells then were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) or GFP-HSV-1 virus, at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. The inoculated Vero cells were evaluated by fluorescence microscopy (Fig. 4). The results showed significant HSV-1 inhibition, as well as inhibition of entry of the adenovirus.\nFigure 4 GA inhibits non-enveloped human adenovirus. (A) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells were infected with GFP-HSV-1 virus at an MOI of 0.5 in medium containing 10 µM GA C15:1 for 24 hours. Infection was evaluated by light (lower panels) and fluorescence (upper panels) microscopy. (B) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM of GA C15:1 or DMSO vehicle. The cells were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. Inhibition of entry was evaluated by light (lower panels) and fluorescence (upper panels) microscopy."}

LitCovid-PD-MONDO

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T51","span":{"begin":906,"end":915},"obj":"Disease"}],"attributes":[{"id":"A51","pred":"mondo_id","subj":"T51","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"To assess the effect of GA on non-enveloped virus, we also tested its antiviral activity on adenovirus, which is internalized by endocytosis. Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells then were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) or GFP-HSV-1 virus, at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. The inoculated Vero cells were evaluated by fluorescence microscopy (Fig. 4). The results showed significant HSV-1 inhibition, as well as inhibition of entry of the adenovirus.\nFigure 4 GA inhibits non-enveloped human adenovirus. (A) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells were infected with GFP-HSV-1 virus at an MOI of 0.5 in medium containing 10 µM GA C15:1 for 24 hours. Infection was evaluated by light (lower panels) and fluorescence (upper panels) microscopy. (B) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM of GA C15:1 or DMSO vehicle. The cells were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. Inhibition of entry was evaluated by light (lower panels) and fluorescence (upper panels) microscopy."}

LitCovid-PD-CLO

LitCovid-PD-CHEBI

LitCovid-PD-GO-BP

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T37","span":{"begin":129,"end":140},"obj":"http://purl.obolibrary.org/obo/GO_0006897"}],"text":"To assess the effect of GA on non-enveloped virus, we also tested its antiviral activity on adenovirus, which is internalized by endocytosis. Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells then were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) or GFP-HSV-1 virus, at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. The inoculated Vero cells were evaluated by fluorescence microscopy (Fig. 4). The results showed significant HSV-1 inhibition, as well as inhibition of entry of the adenovirus.\nFigure 4 GA inhibits non-enveloped human adenovirus. (A) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells were infected with GFP-HSV-1 virus at an MOI of 0.5 in medium containing 10 µM GA C15:1 for 24 hours. Infection was evaluated by light (lower panels) and fluorescence (upper panels) microscopy. (B) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM of GA C15:1 or DMSO vehicle. The cells were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. Inhibition of entry was evaluated by light (lower panels) and fluorescence (upper panels) microscopy."}

LitCovid-sentences

{"project":"LitCovid-sentences","denotations":[{"id":"T99","span":{"begin":0,"end":141},"obj":"Sentence"},{"id":"T100","span":{"begin":142,"end":247},"obj":"Sentence"},{"id":"T101","span":{"begin":248,"end":453},"obj":"Sentence"},{"id":"T102","span":{"begin":454,"end":531},"obj":"Sentence"},{"id":"T103","span":{"begin":532,"end":630},"obj":"Sentence"},{"id":"T104","span":{"begin":631,"end":793},"obj":"Sentence"},{"id":"T105","span":{"begin":794,"end":905},"obj":"Sentence"},{"id":"T106","span":{"begin":906,"end":1110},"obj":"Sentence"},{"id":"T107","span":{"begin":1111,"end":1291},"obj":"Sentence"},{"id":"T108","span":{"begin":1292,"end":1393},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To assess the effect of GA on non-enveloped virus, we also tested its antiviral activity on adenovirus, which is internalized by endocytosis. Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells then were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) or GFP-HSV-1 virus, at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. The inoculated Vero cells were evaluated by fluorescence microscopy (Fig. 4). The results showed significant HSV-1 inhibition, as well as inhibition of entry of the adenovirus.\nFigure 4 GA inhibits non-enveloped human adenovirus. (A) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM GA C15:1 or DMSO vehicle. The cells were infected with GFP-HSV-1 virus at an MOI of 0.5 in medium containing 10 µM GA C15:1 for 24 hours. Infection was evaluated by light (lower panels) and fluorescence (upper panels) microscopy. (B) Monolayers of Vero cells were incubated for 1 hour with medium containing 10 µM of GA C15:1 or DMSO vehicle. The cells were inoculated with a replication-defective human adenovirus type 5 (dE1/E3) containing GFP (Ad-GFP) at an MOI of 0.5 in a medium containing 10 µM GA C15:1 for 24 hours. Inhibition of entry was evaluated by light (lower panels) and fluorescence (upper panels) microscopy."}