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LitCovid-PubTator

LitCovid-PD-FMA-UBERON

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T81","span":{"begin":29,"end":36},"obj":"Body_part"},{"id":"T82","span":{"begin":103,"end":110},"obj":"Body_part"},{"id":"T83","span":{"begin":272,"end":276},"obj":"Body_part"},{"id":"T84","span":{"begin":431,"end":436},"obj":"Body_part"},{"id":"T85","span":{"begin":827,"end":843},"obj":"Body_part"},{"id":"T86","span":{"begin":1068,"end":1076},"obj":"Body_part"},{"id":"T87","span":{"begin":1161,"end":1170},"obj":"Body_part"},{"id":"T88","span":{"begin":1310,"end":1317},"obj":"Body_part"},{"id":"T89","span":{"begin":1434,"end":1441},"obj":"Body_part"},{"id":"T90","span":{"begin":1541,"end":1548},"obj":"Body_part"}],"attributes":[{"id":"A81","pred":"fma_id","subj":"T81","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A82","pred":"fma_id","subj":"T82","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A83","pred":"fma_id","subj":"T83","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A84","pred":"fma_id","subj":"T84","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A85","pred":"fma_id","subj":"T85","obj":"http://purl.org/sig/ont/fma/fma54529"},{"id":"A86","pred":"fma_id","subj":"T86","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A87","pred":"fma_id","subj":"T87","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A88","pred":"fma_id","subj":"T88","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A89","pred":"fma_id","subj":"T89","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A90","pred":"fma_id","subj":"T90","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid_AGAC

{"project":"LitCovid_AGAC","denotations":[{"id":"p12744s11","span":{"begin":205,"end":214},"obj":"CPA"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid-PD-MONDO

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T75","span":{"begin":80,"end":88},"obj":"Disease"},{"id":"T76","span":{"begin":92,"end":96},"obj":"Disease"},{"id":"T77","span":{"begin":165,"end":173},"obj":"Disease"},{"id":"T78","span":{"begin":757,"end":761},"obj":"Disease"},{"id":"T79","span":{"begin":867,"end":875},"obj":"Disease"},{"id":"T80","span":{"begin":937,"end":941},"obj":"Disease"},{"id":"T81","span":{"begin":1233,"end":1237},"obj":"Disease"},{"id":"T82","span":{"begin":1484,"end":1492},"obj":"Disease"},{"id":"T83","span":{"begin":1497,"end":1501},"obj":"Disease"}],"attributes":[{"id":"A75","pred":"mondo_id","subj":"T75","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A76","pred":"mondo_id","subj":"T76","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A77","pred":"mondo_id","subj":"T77","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A78","pred":"mondo_id","subj":"T78","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A79","pred":"mondo_id","subj":"T79","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A80","pred":"mondo_id","subj":"T80","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A81","pred":"mondo_id","subj":"T81","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A82","pred":"mondo_id","subj":"T82","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A83","pred":"mondo_id","subj":"T83","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid-PD-CLO

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T194","span":{"begin":6,"end":7},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T195","span":{"begin":255,"end":260},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T196","span":{"begin":272,"end":282},"obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"T197","span":{"begin":377,"end":378},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T198","span":{"begin":410,"end":414},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T199","span":{"begin":410,"end":414},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T200","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T201","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T202","span":{"begin":426,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T203","span":{"begin":431,"end":436},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T204","span":{"begin":454,"end":455},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T205","span":{"begin":683,"end":693},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T206","span":{"begin":753,"end":756},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T207","span":{"begin":857,"end":863},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T208","span":{"begin":877,"end":879},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T209","span":{"begin":897,"end":905},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T210","span":{"begin":1011,"end":1021},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T211","span":{"begin":1110,"end":1120},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T212","span":{"begin":1204,"end":1205},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T213","span":{"begin":1229,"end":1232},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T214","span":{"begin":1403,"end":1404},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T215","span":{"begin":1515,"end":1516},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T216","span":{"begin":1518,"end":1519},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid-PD-CHEBI

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T112","span":{"begin":29,"end":36},"obj":"Chemical"},{"id":"T113","span":{"begin":103,"end":110},"obj":"Chemical"},{"id":"T114","span":{"begin":836,"end":843},"obj":"Chemical"},{"id":"T115","span":{"begin":886,"end":896},"obj":"Chemical"},{"id":"T116","span":{"begin":980,"end":990},"obj":"Chemical"},{"id":"T117","span":{"begin":1068,"end":1076},"obj":"Chemical"},{"id":"T118","span":{"begin":1099,"end":1109},"obj":"Chemical"},{"id":"T119","span":{"begin":1310,"end":1317},"obj":"Chemical"},{"id":"T120","span":{"begin":1434,"end":1441},"obj":"Chemical"},{"id":"T121","span":{"begin":1541,"end":1548},"obj":"Chemical"}],"attributes":[{"id":"A112","pred":"chebi_id","subj":"T112","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A113","pred":"chebi_id","subj":"T113","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A114","pred":"chebi_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/CHEBI_33252"},{"id":"A115","pred":"chebi_id","subj":"T115","obj":"http://purl.obolibrary.org/obo/CHEBI_48706"},{"id":"A116","pred":"chebi_id","subj":"T116","obj":"http://purl.obolibrary.org/obo/CHEBI_48706"},{"id":"A117","pred":"chebi_id","subj":"T117","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A118","pred":"chebi_id","subj":"T118","obj":"http://purl.obolibrary.org/obo/CHEBI_48706"},{"id":"A119","pred":"chebi_id","subj":"T119","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A120","pred":"chebi_id","subj":"T120","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A121","pred":"chebi_id","subj":"T121","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid-PD-GO-BP

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T10","span":{"begin":330,"end":347},"obj":"http://purl.obolibrary.org/obo/GO_0019079"},{"id":"T11","span":{"begin":330,"end":347},"obj":"http://purl.obolibrary.org/obo/GO_0019058"},{"id":"T12","span":{"begin":493,"end":501},"obj":"http://purl.obolibrary.org/obo/GO_0070265"},{"id":"T13","span":{"begin":493,"end":501},"obj":"http://purl.obolibrary.org/obo/GO_0019835"},{"id":"T14","span":{"begin":493,"end":501},"obj":"http://purl.obolibrary.org/obo/GO_0008219"},{"id":"T15","span":{"begin":493,"end":501},"obj":"http://purl.obolibrary.org/obo/GO_0001906"},{"id":"T16","span":{"begin":564,"end":572},"obj":"http://purl.obolibrary.org/obo/GO_0070265"},{"id":"T17","span":{"begin":564,"end":572},"obj":"http://purl.obolibrary.org/obo/GO_0019835"},{"id":"T18","span":{"begin":564,"end":572},"obj":"http://purl.obolibrary.org/obo/GO_0008219"},{"id":"T19","span":{"begin":564,"end":572},"obj":"http://purl.obolibrary.org/obo/GO_0001906"},{"id":"T20","span":{"begin":577,"end":586},"obj":"http://purl.obolibrary.org/obo/GO_0097194"},{"id":"T21","span":{"begin":577,"end":586},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T22","span":{"begin":669,"end":678},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T23","span":{"begin":683,"end":693},"obj":"http://purl.obolibrary.org/obo/GO_0023052"},{"id":"T24","span":{"begin":836,"end":856},"obj":"http://purl.obolibrary.org/obo/GO_0051647"},{"id":"T25","span":{"begin":844,"end":856},"obj":"http://purl.obolibrary.org/obo/GO_0051179"},{"id":"T26","span":{"begin":1179,"end":1191},"obj":"http://purl.obolibrary.org/obo/GO_0051179"},{"id":"T27","span":{"begin":1269,"end":1281},"obj":"http://purl.obolibrary.org/obo/GO_0009405"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

LitCovid-sentences

{"project":"LitCovid-sentences","denotations":[{"id":"T181","span":{"begin":0,"end":5},"obj":"Sentence"},{"id":"T182","span":{"begin":6,"end":145},"obj":"Sentence"},{"id":"T183","span":{"begin":146,"end":357},"obj":"Sentence"},{"id":"T184","span":{"begin":358,"end":613},"obj":"Sentence"},{"id":"T185","span":{"begin":614,"end":699},"obj":"Sentence"},{"id":"T186","span":{"begin":700,"end":881},"obj":"Sentence"},{"id":"T187","span":{"begin":882,"end":1022},"obj":"Sentence"},{"id":"T188","span":{"begin":1023,"end":1282},"obj":"Sentence"},{"id":"T189","span":{"begin":1283,"end":1373},"obj":"Sentence"},{"id":"T190","span":{"begin":1374,"end":1383},"obj":"Sentence"},{"id":"T191","span":{"begin":1384,"end":1402},"obj":"Sentence"},{"id":"T192","span":{"begin":1403,"end":1405},"obj":"Sentence"},{"id":"T193","span":{"begin":1406,"end":1514},"obj":"Sentence"},{"id":"T194","span":{"begin":1515,"end":1517},"obj":"Sentence"},{"id":"T195","span":{"begin":1518,"end":1564},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

2_test

{"project":"2_test","denotations":[{"id":"31987001-16282490-27754487","span":{"begin":284,"end":286},"obj":"16282490"},{"id":"31987001-16965829-27754488","span":{"begin":609,"end":611},"obj":"16965829"},{"id":"31987001-17108024-27754489","span":{"begin":695,"end":697},"obj":"17108024"},{"id":"31987001-22012463-27754490","span":{"begin":877,"end":879},"obj":"22012463"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

MyTest

{"project":"MyTest","denotations":[{"id":"31987001-16282490-27754487","span":{"begin":284,"end":286},"obj":"16282490"},{"id":"31987001-16965829-27754488","span":{"begin":609,"end":611},"obj":"16965829"},{"id":"31987001-17108024-27754489","span":{"begin":695,"end":697},"obj":"17108024"},{"id":"31987001-22012463-27754490","span":{"begin":877,"end":879},"obj":"22012463"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"Orf3b\nA novel short putative protein with 4 helices and no homology to existing SARS-CoV or SARS-r-CoV protein was found within Orf3b (Figure 4). It is notable that SARS-CoV deletion mutants lacking orf3b replicate to levels similar to those of wild-type virus in several cell types [19], suggesting that orf3b is dispensable for viral replication in vitro. But orf3b may have a role in viral pathogenicity as Vero E6 but not 293T cells transfected with a construct expressing Orf3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time points [20]. Orf3b was also shown to inhibit expression of IFN-β at synthesis and signalling [21]. Subsequently, orf3b homologues identified from three bat SARS-related-CoV strains were C-terminally truncated and lacked the C-terminal nucleus localization signal of SARS-CoV [22]. IFN antagonist activity analysis demonstrated that one SARS-related-CoV orf3b still possessed IFN antagonist and IRF3-modulating activities. These results indicated that different orf3b proteins display different IFN antagonist activities and this function is independent of the protein's nuclear localization, suggesting a potential link between bat SARS-related-CoV orf3b function and pathogenesis. The importance of this new protein in 2019-nCoV will require further validation and study.\nFigure 4. Analysis of orf3b. A. Multiple alignment of orf3b protein sequence between 2019-nCoV (HKU-SZ-005b), SARS-CoV and SARS-related CoV. B. A novel putative short protein found in orf3b."}

PMC:7067204 / 16949-18513 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:7067204 / 16949-18513 JSON TXT