PMC:7045880 / 87-1366 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7045880","sourcedb":"PMC","sourceid":"7045880","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7045880","text":"Objectives\nFollowing reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.\n\nMethods\nUpper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.\n\nResults\nThe virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.\n\nConclusion\nSARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. 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