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LitCovid-PubTator

{"project":"LitCovid-PubTator","denotations":[{"id":"159","span":{"begin":129,"end":145},"obj":"Chemical"},{"id":"160","span":{"begin":155,"end":169},"obj":"Chemical"},{"id":"161","span":{"begin":179,"end":188},"obj":"Chemical"},{"id":"162","span":{"begin":434,"end":450},"obj":"Chemical"},{"id":"163","span":{"begin":489,"end":494},"obj":"Chemical"},{"id":"164","span":{"begin":600,"end":607},"obj":"Chemical"},{"id":"165","span":{"begin":702,"end":717},"obj":"Chemical"},{"id":"166","span":{"begin":922,"end":928},"obj":"Chemical"},{"id":"167","span":{"begin":962,"end":976},"obj":"Chemical"},{"id":"168","span":{"begin":243,"end":251},"obj":"Disease"},{"id":"169","span":{"begin":662,"end":673},"obj":"Disease"}],"attributes":[{"id":"A159","pred":"tao:has_database_id","subj":"159","obj":"MESH:C003043"},{"id":"A160","pred":"tao:has_database_id","subj":"160","obj":"MESH:D005976"},{"id":"A161","pred":"tao:has_database_id","subj":"161","obj":"MESH:D010710"},{"id":"A162","pred":"tao:has_database_id","subj":"162","obj":"MESH:D009993"},{"id":"A163","pred":"tao:has_database_id","subj":"163","obj":"MESH:D014867"},{"id":"A164","pred":"tao:has_database_id","subj":"164","obj":"MESH:D000431"},{"id":"A165","pred":"tao:has_database_id","subj":"165","obj":"MESH:C009068"},{"id":"A166","pred":"tao:has_database_id","subj":"166","obj":"MESH:D009532"},{"id":"A167","pred":"tao:has_database_id","subj":"167","obj":"MESH:C005460"},{"id":"A168","pred":"tao:has_database_id","subj":"168","obj":"MESH:D007239"},{"id":"A169","pred":"tao:has_database_id","subj":"169","obj":"MESH:D003681"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

LitCovid-PD-FMA-UBERON

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T33","span":{"begin":89,"end":94},"obj":"Body_part"},{"id":"T34","span":{"begin":237,"end":242},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A34","pred":"fma_id","subj":"T34","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

LitCovid-PD-CLO

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T64","span":{"begin":89,"end":94},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T65","span":{"begin":237,"end":242},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T66","span":{"begin":257,"end":262},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T67","span":{"begin":496,"end":498},"obj":"http://purl.obolibrary.org/obo/CLO_0037161"},{"id":"T68","span":{"begin":803,"end":805},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T69","span":{"begin":911,"end":912},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T70","span":{"begin":1010,"end":1011},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

LitCovid-PD-CHEBI

LitCovid-PD-GO-BP

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T10","span":{"begin":220,"end":229},"obj":"http://purl.obolibrary.org/obo/GO_0097264"},{"id":"T11","span":{"begin":220,"end":229},"obj":"http://purl.obolibrary.org/obo/GO_0001896"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

2_test

{"project":"2_test","denotations":[{"id":"32149036-26235643-81764452","span":{"begin":1115,"end":1117},"obj":"26235643"},{"id":"32149036-19844604-81764452","span":{"begin":1115,"end":1117},"obj":"19844604"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

LitCovid-sentences

{"project":"LitCovid-sentences","denotations":[{"id":"T64","span":{"begin":0,"end":2},"obj":"Sentence"},{"id":"T65","span":{"begin":3,"end":35},"obj":"Sentence"},{"id":"T66","span":{"begin":36,"end":263},"obj":"Sentence"},{"id":"T67","span":{"begin":264,"end":451},"obj":"Sentence"},{"id":"T68","span":{"begin":452,"end":553},"obj":"Sentence"},{"id":"T69","span":{"begin":554,"end":718},"obj":"Sentence"},{"id":"T70","span":{"begin":719,"end":812},"obj":"Sentence"},{"id":"T71","span":{"begin":813,"end":977},"obj":"Sentence"},{"id":"T72","span":{"begin":978,"end":1122},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

LitCovid-PD-HP

{"project":"LitCovid-PD-HP","denotations":[{"id":"T8","span":{"begin":662,"end":673},"obj":"Phenotype"}],"attributes":[{"id":"A8","pred":"hp_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/HP_0001944"}],"text":"6. Transmission electron microscopy\nFor transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]."}

PMC:7045880 / 6735-7857 JSONTXT

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PMC:7045880 / 6735-7857 JSON TXT