PMC:7036342 / 3069-4007 JSONTXT

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Until 5 days after inoculation, cytopathic effects were not distinct, which is compatible with the previous findings that no specific cytopathic effects were observed in the Vero E6 cells until 6 days after inoculation in the report about first isolation of SARS-CoV-2.3 Five days after inoculation, we did blind passage of culture supernatant into T-25 culture flask (ThermoFisher Scientific Inc., Waltham, MA, USA) with monolayers of Vero cells, and cytopathic effects consisting of rounding and detachment of cells were observed in the whole area of the T-25 flask 3 days after the first blind passage (Fig. 1A and B).","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"96","span":{"begin":4,"end":11},"obj":"Species"},{"id":"97","span":{"begin":575,"end":585},"obj":"Species"},{"id":"98","span":{"begin":225,"end":231},"obj":"Chemical"},{"id":"99","span":{"begin":290,"end":304},"obj":"Chemical"}],"attributes":[{"id":"A96","pred":"tao:has_database_id","subj":"96","obj":"Tax:9606"},{"id":"A97","pred":"tao:has_database_id","subj":"97","obj":"Tax:2697049"},{"id":"A99","pred":"tao:has_database_id","subj":"99","obj":"MESH:D002245"},{"subj":"96","pred":"source","obj":"LitCovid-PubTator"},{"subj":"97","pred":"source","obj":"LitCovid-PubTator"},{"subj":"98","pred":"source","obj":"LitCovid-PubTator"},{"subj":"99","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T124","span":{"begin":14,"end":27},"obj":"UBERON:0001179"},{"id":"T123","span":{"begin":87,"end":92},"obj":"NCBITaxon:10239"},{"id":"T122","span":{"begin":206,"end":210},"obj":"CL_6"},{"id":"T121","span":{"begin":297,"end":304},"obj":"CHEBI:16526;CHEBI:16526"},{"id":"T120","span":{"begin":491,"end":495},"obj":"CL_6"},{"id":"T119","span":{"begin":575,"end":585},"obj":"SP_7"},{"id":"T118","span":{"begin":753,"end":757},"obj":"CL_6"}],"attributes":[{"subj":"T124","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T123","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T122","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T121","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T120","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T119","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T118","pred":"source","obj":"LitCovid-PMC-OGER-BB"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T16","span":{"begin":211,"end":216},"obj":"Body_part"},{"id":"T17","span":{"begin":268,"end":273},"obj":"Body_part"},{"id":"T18","span":{"begin":499,"end":504},"obj":"Body_part"},{"id":"T19","span":{"begin":758,"end":763},"obj":"Body_part"},{"id":"T20","span":{"begin":829,"end":834},"obj":"Body_part"}],"attributes":[{"id":"A16","pred":"fma_id","subj":"T16","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A17","pred":"fma_id","subj":"T17","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A18","pred":"fma_id","subj":"T18","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A19","pred":"fma_id","subj":"T19","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A20","pred":"fma_id","subj":"T20","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T35","span":{"begin":575,"end":583},"obj":"Disease"},{"id":"T36","span":{"begin":575,"end":579},"obj":"Disease"}],"attributes":[{"id":"A35","pred":"mondo_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A36","pred":"mondo_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T35","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T33","span":{"begin":206,"end":210},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T34","span":{"begin":206,"end":210},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T35","span":{"begin":211,"end":216},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T36","span":{"begin":255,"end":273},"obj":"http://purl.obolibrary.org/obo/CL_0000010"},{"id":"T37","span":{"begin":285,"end":286},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T38","span":{"begin":491,"end":504},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T39","span":{"begin":753,"end":757},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T40","span":{"begin":753,"end":757},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T41","span":{"begin":758,"end":763},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T42","span":{"begin":829,"end":834},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T43","span":{"begin":935,"end":936},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"}],"attributes":[{"subj":"T33","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T34","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T35","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T37","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T38","pred":"source","obj":"LitCovid-PD-CLO"},{"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