PMC:7025476 / 6025-7068
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7025476","sourcedb":"PMC","sourceid":"7025476","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7025476","text":"OVA-Induced Allergic Airway Inflammation and Interventions\nThe murine model of allergic airway inflammation was established according to previous study (27). Briefly, the mice (6–8 weeks old) were sensitized on day 0 with an intra-peritoneal injection of OVA (Sigma, St. Louis, MO, USA) emulsified with 1 mg potassium aluminum sulfate (Sangon Biotech, Shanghai, China) in 500 μl of saline. Airway inflammation was induced by inhalation of 1% aerosolized OVA 30 min per day, for consecutive 7 days. Control mice received saline-sensitization and inhalation of nebulized saline solution. The mice from WT-OVA-Melatonin group were administered with melatonin (10 mg/kg, Sigma), and the mice from TLR2−/−-OVA-Luzindole group were administered with luzindole (30 mg/kg, Sigma) 1 h before allergen-challenge through intra-peritoneal injection, respectively, the dose of melatonin and luzindole was used according to previous studies (19, 28). Experiments were performed with six mice per group. Mice were harvested 24 h following the last challenge.","divisions":[{"label":"title","span":{"begin":0,"end":58}}],"tracks":[{"project":"TEST0","denotations":[{"id":"32117301-94-100-3829154","span":{"begin":153,"end":155},"obj":"[\"17237192\"]"},{"id":"32117301-235-241-3829155","span":{"begin":928,"end":930},"obj":"[\"25161126\"]"}],"attributes":[{"subj":"32117301-94-100-3829154","pred":"source","obj":"TEST0"},{"subj":"32117301-235-241-3829155","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"32117301-17237192-35348479","span":{"begin":153,"end":155},"obj":"17237192"},{"id":"32117301-25161126-35348480","span":{"begin":928,"end":930},"obj":"25161126"}],"attributes":[{"subj":"32117301-17237192-35348479","pred":"source","obj":"2_test"},{"subj":"32117301-25161126-35348480","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"32117301-17237192-35348479","span":{"begin":153,"end":155},"obj":"17237192"},{"id":"32117301-25161126-35348480","span":{"begin":928,"end":930},"obj":"25161126"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32117301-17237192-35348479","pred":"source","obj":"MyTest"},{"subj":"32117301-25161126-35348480","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#d393ec","default":true},{"id":"2_test","color":"#93ecb9"},{"id":"MyTest","color":"#ec9f93"}]}]}}