PMC:7025468 / 5786-6717 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7025468","sourcedb":"PMC","sourceid":"7025468","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7025468","text":"Reagents, Treatment of Cells\nFor methamphetamine (Meth) treatment, cells were plated at an initial density of 2 × 106cells/mL. Meth was administered once per day at a final concentration of 100 μM, consistent with observed concentrations of Meth in samples from Meth abusers (35). For cultures with HIV-1, cells were pretreated with Meth for 24 h, followed by infection with HIV-1. Cells treated with IL-1RA were given 200 or 400 ng/mL IL-1RA as described (Shenandoah Biotechnology Inc., Warwick, PA), followed by treatment with 100 μM Meth 2 h later if applicable. Cells treated with both HIV-1 and IL-1RA were pretreated for 24 h with IL-1RA before becoming infected with HIV-1. Cells treated with IFNα2 were administered 100U/mL IFNα2 (PBL Assay Science, Piscataway, NJ) for 2 h prior to treatment with 100 μM Meth. Cells treated with both HIV-1 and IFNα2 were pretreated for 24 h with IFNα2 before becoming infected with HIV-1.","divisions":[{"label":"title","span":{"begin":0,"end":28}}],"tracks":[{"project":"TEST0","denotations":[{"id":"32117283-149-155-3683892","span":{"begin":276,"end":278},"obj":"[\"20668216\"]"}],"attributes":[{"subj":"32117283-149-155-3683892","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"32117283-20668216-35221698","span":{"begin":276,"end":278},"obj":"20668216"}],"attributes":[{"subj":"32117283-20668216-35221698","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"32117283-20668216-35221698","span":{"begin":276,"end":278},"obj":"20668216"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32117283-20668216-35221698","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#939dec","default":true},{"id":"2_test","color":"#b7ec93"},{"id":"MyTest","color":"#ec93d1"}]}]}}