PMC:7025468 / 14198-14933 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7025468","sourcedb":"PMC","sourceid":"7025468","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7025468","text":"Healthy donor CD4+ T-cells were treated daily with 100 μM Meth, and miR-146a expression was assessed. We observed that Meth significantly up-regulated miR-146a on day 3 of treatment (Figure 2A). Likewise, we assessed IL-1β mRNA levels in untreated and Meth treated cells. Unlike extracellular IL-1β, which increased after 1 day of Meth treatment, IL-1β mRNA showed increased expression only on day 3 (Figure 2A). Notably, IL-1β release and mRNA expression are controlled by distinct mechanisms (45). In addition, CD4+ T-cells constitutively express pro-IL-1β in their cytoplasm (46). As such, our results indicate that Meth first enhances release of mature IL-1β, followed by increased IL-1β gene transcription and miR-146a expression.","tracks":[{"project":"TEST0","denotations":[{"id":"32117283-82-88-3683908","span":{"begin":495,"end":497},"obj":"[\"30300855\"]"},{"id":"32117283-79-85-3683909","span":{"begin":579,"end":581},"obj":"[\"24356306\"]"}],"attributes":[{"subj":"32117283-82-88-3683908","pred":"source","obj":"TEST0"},{"subj":"32117283-79-85-3683909","pred":"source","obj":"TEST0"}]},{"project":"2_test","denotations":[{"id":"32117283-30300855-35221714","span":{"begin":495,"end":497},"obj":"30300855"},{"id":"32117283-24356306-35221715","span":{"begin":579,"end":581},"obj":"24356306"}],"attributes":[{"subj":"32117283-30300855-35221714","pred":"source","obj":"2_test"},{"subj":"32117283-24356306-35221715","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"32117283-30300855-35221714","span":{"begin":495,"end":497},"obj":"30300855"},{"id":"32117283-24356306-35221715","span":{"begin":579,"end":581},"obj":"24356306"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32117283-30300855-35221714","pred":"source","obj":"MyTest"},{"subj":"32117283-24356306-35221715","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#99ec93","default":true},{"id":"2_test","color":"#ec93b4"},{"id":"MyTest","color":"#93ceec"}]}]}}