PMC:7019868 / 5619-6464 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"99","span":{"begin":115,"end":119},"obj":"Species"},{"id":"100","span":{"begin":387,"end":394},"obj":"Chemical"},{"id":"101","span":{"begin":460,"end":467},"obj":"Chemical"},{"id":"102","span":{"begin":536,"end":543},"obj":"Chemical"},{"id":"103","span":{"begin":544,"end":547},"obj":"Chemical"}],"attributes":[{"id":"A99","pred":"tao:has_database_id","subj":"99","obj":"Tax:28295"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In the present study, we utilized reverse genetics to examine the role of the S gene in the attenuation process of PEDV using the highly virulent and attenuated G2b PEDV Pintung (PEDVPT) 52 strains, the PEDVPT-P5 and PEDVPT-P96 viruses. Nonetheless, our findings were discordant with previous reported data [17]. We found that replacement of the S gene with the iPEDVPT-P5 virus enabled iPEDVPT-P96 to regain its virulence. A reciprocal approach revealed that iPEDVPT-P5 virus became partly attenuated after the S gene was exchanged to iPEDVPT-P96. Collectively, we concluded that the S gene is of critical importance to the attenuation process of the PEDVPT 52 strain. However, mutation in the S gene alone could not completely attenuate the iPEDVPT-P5 virus. Thus, gene(s) other than S gene could also play a role in determining the virulence."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T28","span":{"begin":80,"end":84},"obj":"Body_part"},{"id":"T29","span":{"begin":348,"end":352},"obj":"Body_part"},{"id":"T30","span":{"begin":514,"end":518},"obj":"Body_part"},{"id":"T31","span":{"begin":587,"end":591},"obj":"Body_part"},{"id":"T32","span":{"begin":697,"end":701},"obj":"Body_part"},{"id":"T33","span":{"begin":767,"end":771},"obj":"Body_part"},{"id":"T34","span":{"begin":788,"end":792},"obj":"Body_part"}],"attributes":[{"id":"A28","pred":"fma_id","subj":"T28","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A29","pred":"fma_id","subj":"T29","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A30","pred":"fma_id","subj":"T30","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A32","pred":"fma_id","subj":"T32","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A34","pred":"fma_id","subj":"T34","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"In the present study, we utilized reverse genetics to examine the role of the S gene in the attenuation process of PEDV using the highly virulent and attenuated G2b PEDV Pintung (PEDVPT) 52 strains, the PEDVPT-P5 and PEDVPT-P96 viruses. Nonetheless, our findings were discordant with previous reported data [17]. We found that replacement of the S gene with the iPEDVPT-P5 virus enabled iPEDVPT-P96 to regain its virulence. A reciprocal approach revealed that iPEDVPT-P5 virus became partly attenuated after the S gene was exchanged to iPEDVPT-P96. Collectively, we concluded that the S gene is of critical importance to the attenuation process of the PEDVPT 52 strain. However, mutation in the S gene alone could not completely attenuate the iPEDVPT-P5 virus. Thus, gene(s) other than S gene could also play a role in determining the virulence."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T57","span":{"begin":80,"end":84},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T58","span":{"begin":187,"end":189},"obj":"http://purl.obolibrary.org/obo/CLO_0001407"},{"id":"T59","span":{"begin":228,"end":235},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T60","span":{"begin":348,"end":352},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T61","span":{"begin":373,"end":378},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T62","span":{"begin":424,"end":425},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T63","span":{"begin":471,"end":476},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T64","span":{"begin":514,"end":518},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T65","span":{"begin":587,"end":591},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T66","span":{"begin":659,"end":661},"obj":"http://purl.obolibrary.org/obo/CLO_0001407"},{"id":"T67","span":{"begin":697,"end":701},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T68","span":{"begin":754,"end":759},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T69","span":{"begin":767,"end":771},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T70","span":{"begin":788,"end":792},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T71","span":{"begin":809,"end":810},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"In the present study, we utilized reverse genetics to examine the role of the S gene in the attenuation process of PEDV using the highly virulent and attenuated G2b PEDV Pintung (PEDVPT) 52 strains, the PEDVPT-P5 and PEDVPT-P96 viruses. Nonetheless, our findings were discordant with previous reported data [17]. We found that replacement of the S gene with the iPEDVPT-P5 virus enabled iPEDVPT-P96 to regain its virulence. A reciprocal approach revealed that iPEDVPT-P5 virus became partly attenuated after the S gene was exchanged to iPEDVPT-P96. Collectively, we concluded that the S gene is of critical importance to the attenuation process of the PEDVPT 52 strain. However, mutation in the S gene alone could not completely attenuate the iPEDVPT-P5 virus. Thus, gene(s) other than S gene could also play a role in determining the virulence."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T21","span":{"begin":413,"end":422},"obj":"http://purl.obolibrary.org/obo/GO_0016032"},{"id":"T22","span":{"begin":413,"end":422},"obj":"http://purl.obolibrary.org/obo/GO_0009405"},{"id":"T23","span":{"begin":835,"end":844},"obj":"http://purl.obolibrary.org/obo/GO_0016032"},{"id":"T24","span":{"begin":835,"end":844},"obj":"http://purl.obolibrary.org/obo/GO_0009405"}],"text":"In the present study, we utilized reverse genetics to examine the role of the S gene in the attenuation process of PEDV using the highly virulent and attenuated G2b PEDV Pintung (PEDVPT) 52 strains, the PEDVPT-P5 and PEDVPT-P96 viruses. Nonetheless, our findings were discordant with previous reported data [17]. We found that replacement of the S gene with the iPEDVPT-P5 virus enabled iPEDVPT-P96 to regain its virulence. A reciprocal approach revealed that iPEDVPT-P5 virus became partly attenuated after the S gene was exchanged to iPEDVPT-P96. Collectively, we concluded that the S gene is of critical importance to the attenuation process of the PEDVPT 52 strain. However, mutation in the S gene alone could not completely attenuate the iPEDVPT-P5 virus. Thus, gene(s) other than S gene could also play a role in determining the virulence."}

{"project":"LitCovid-sentences","denotations":[{"id":"T35","span":{"begin":0,"end":236},"obj":"Sentence"},{"id":"T36","span":{"begin":237,"end":312},"obj":"Sentence"},{"id":"T37","span":{"begin":313,"end":423},"obj":"Sentence"},{"id":"T38","span":{"begin":424,"end":548},"obj":"Sentence"},{"id":"T39","span":{"begin":549,"end":669},"obj":"Sentence"},{"id":"T40","span":{"begin":670,"end":760},"obj":"Sentence"},{"id":"T41","span":{"begin":761,"end":845},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In the present study, we utilized reverse genetics to examine the role of the S gene in the attenuation process of PEDV using the highly virulent and attenuated G2b PEDV Pintung (PEDVPT) 52 strains, the PEDVPT-P5 and PEDVPT-P96 viruses. Nonetheless, our findings were discordant with previous reported data [17]. We found that replacement of the S gene with the iPEDVPT-P5 virus enabled iPEDVPT-P96 to regain its virulence. A reciprocal approach revealed that iPEDVPT-P5 virus became partly attenuated after the S gene was exchanged to iPEDVPT-P96. Collectively, we concluded that the S gene is of critical importance to the attenuation process of the PEDVPT 52 strain. However, mutation in the S gene alone could not completely attenuate the iPEDVPT-P5 virus. Thus, gene(s) other than S gene could also play a role in determining the virulence."}