PMC:6988269 / 16296-16988 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T78","span":{"begin":0,"end":4},"obj":"Body_part"},{"id":"T79","span":{"begin":269,"end":272},"obj":"Body_part"},{"id":"T80","span":{"begin":372,"end":375},"obj":"Body_part"},{"id":"T81","span":{"begin":480,"end":484},"obj":"Body_part"},{"id":"T82","span":{"begin":594,"end":600},"obj":"Body_part"}],"attributes":[{"id":"A78","pred":"fma_id","subj":"T78","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A79","pred":"fma_id","subj":"T79","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A80","pred":"fma_id","subj":"T80","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A81","pred":"fma_id","subj":"T81","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A82","pred":"fma_id","subj":"T82","obj":"http://purl.org/sig/ont/fma/fma312401"}],"text":"Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. These were additionally mixed into negative human sputum samples. None of the tested viruses or virus preparations showed reactivity with any assay."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T5","span":{"begin":594,"end":600},"obj":"Body_part"}],"attributes":[{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0007311"}],"text":"Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. These were additionally mixed into negative human sputum samples. None of the tested viruses or virus preparations showed reactivity with any assay."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T146","span":{"begin":0,"end":4},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T147","span":{"begin":49,"end":54},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T148","span":{"begin":130,"end":136},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T149","span":{"begin":232,"end":237},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T150","span":{"begin":238,"end":244},"obj":"http://purl.obolibrary.org/obo/UBERON_0001005"},{"id":"T151","span":{"begin":480,"end":484},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T152","span":{"begin":511,"end":517},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T153","span":{"begin":588,"end":593},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T154","span":{"begin":622,"end":628},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T155","span":{"begin":629,"end":636},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T156","span":{"begin":640,"end":645},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"text":"Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. These were additionally mixed into negative human sputum samples. None of the tested viruses or virus preparations showed reactivity with any assay."}

{"project":"LitCovid-sentences","denotations":[{"id":"T139","span":{"begin":0,"end":180},"obj":"Sentence"},{"id":"T140","span":{"begin":181,"end":262},"obj":"Sentence"},{"id":"T141","span":{"begin":263,"end":437},"obj":"Sentence"},{"id":"T142","span":{"begin":438,"end":543},"obj":"Sentence"},{"id":"T143","span":{"begin":544,"end":609},"obj":"Sentence"},{"id":"T144","span":{"begin":610,"end":692},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. These were additionally mixed into negative human sputum samples. None of the tested viruses or virus preparations showed reactivity with any assay."}

{"project":"LitCovid-PubTator","denotations":[{"id":"287","span":{"begin":89,"end":104},"obj":"Gene"},{"id":"288","span":{"begin":49,"end":54},"obj":"Species"},{"id":"289","span":{"begin":55,"end":68},"obj":"Species"},{"id":"290","span":{"begin":70,"end":80},"obj":"Species"},{"id":"291","span":{"begin":116,"end":124},"obj":"Species"},{"id":"292","span":{"begin":204,"end":213},"obj":"Species"},{"id":"293","span":{"begin":232,"end":237},"obj":"Species"},{"id":"294","span":{"begin":588,"end":593},"obj":"Species"},{"id":"295","span":{"begin":83,"end":87},"obj":"Species"}],"attributes":[{"id":"A288","pred":"tao:has_database_id","subj":"288","obj":"Tax:9606"},{"id":"A289","pred":"tao:has_database_id","subj":"289","obj":"Tax:11118"},{"id":"A290","pred":"tao:has_database_id","subj":"290","obj":"Tax:11137"},{"id":"A291","pred":"tao:has_database_id","subj":"291","obj":"Tax:1335626"},{"id":"A292","pred":"tao:has_database_id","subj":"292","obj":"Tax:290028"},{"id":"A293","pred":"tao:has_database_id","subj":"293","obj":"Tax:9606"},{"id":"A294","pred":"tao:has_database_id","subj":"294","obj":"Tax:9606"},{"id":"A295","pred":"tao:has_database_id","subj":"295","obj":"Tax:277944"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. These were additionally mixed into negative human sputum samples. None of the tested viruses or virus preparations showed reactivity with any assay."}