PMC:6988269 / 14522-15115 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T73","span":{"begin":38,"end":41},"obj":"Body_part"},{"id":"T74","span":{"begin":157,"end":160},"obj":"Body_part"},{"id":"T75","span":{"begin":553,"end":556},"obj":"Body_part"},{"id":"T76","span":{"begin":589,"end":592},"obj":"Body_part"}],"attributes":[{"id":"A73","pred":"fma_id","subj":"T73","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A74","pred":"fma_id","subj":"T74","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A76","pred":"fma_id","subj":"T76","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T45","span":{"begin":29,"end":37},"obj":"Disease"},{"id":"T46","span":{"begin":29,"end":33},"obj":"Disease"},{"id":"T47","span":{"begin":257,"end":265},"obj":"Disease"},{"id":"T48","span":{"begin":257,"end":261},"obj":"Disease"},{"id":"T49","span":{"begin":580,"end":588},"obj":"Disease"},{"id":"T50","span":{"begin":580,"end":584},"obj":"Disease"}],"attributes":[{"id":"A45","pred":"mondo_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A46","pred":"mondo_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A49","pred":"mondo_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A50","pred":"mondo_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T126","span":{"begin":57,"end":58},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T127","span":{"begin":223,"end":224},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T128","span":{"begin":485,"end":490},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T129","span":{"begin":522,"end":524},"obj":"http://purl.obolibrary.org/obo/CLO_0008307"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T44","span":{"begin":237,"end":242},"obj":"Chemical"},{"id":"T45","span":{"begin":298,"end":303},"obj":"Chemical"},{"id":"T46","span":{"begin":505,"end":510},"obj":"Chemical"},{"id":"T47","span":{"begin":522,"end":524},"obj":"Chemical"}],"attributes":[{"id":"A44","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A45","pred":"chebi_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A46","pred":"chebi_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A47","pred":"chebi_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/CHEBI_33472"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T12","span":{"begin":557,"end":567},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-sentences","denotations":[{"id":"T125","span":{"begin":0,"end":336},"obj":"Sentence"},{"id":"T126","span":{"begin":337,"end":491},"obj":"Sentence"},{"id":"T127","span":{"begin":492,"end":593},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PubTator","denotations":[{"id":"256","span":{"begin":180,"end":184},"obj":"Gene"},{"id":"257","span":{"begin":29,"end":37},"obj":"Species"},{"id":"258","span":{"begin":147,"end":156},"obj":"Species"},{"id":"259","span":{"begin":257,"end":265},"obj":"Species"},{"id":"260","span":{"begin":270,"end":279},"obj":"Species"},{"id":"261","span":{"begin":326,"end":335},"obj":"Species"},{"id":"262","span":{"begin":543,"end":552},"obj":"Species"},{"id":"263","span":{"begin":580,"end":588},"obj":"Species"}],"attributes":[{"id":"A256","pred":"tao:has_database_id","subj":"256","obj":"Gene:43740578"},{"id":"A257","pred":"tao:has_database_id","subj":"257","obj":"Tax:694009"},{"id":"A258","pred":"tao:has_database_id","subj":"258","obj":"Tax:2697049"},{"id":"A259","pred":"tao:has_database_id","subj":"259","obj":"Tax:694009"},{"id":"A260","pred":"tao:has_database_id","subj":"260","obj":"Tax:2697049"},{"id":"A261","pred":"tao:has_database_id","subj":"261","obj":"Tax:2697049"},{"id":"A262","pred":"tao:has_database_id","subj":"262","obj":"Tax:2697049"},{"id":"A263","pred":"tao:has_database_id","subj":"263","obj":"Tax:694009"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Following the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}