PMC:6988269 / 14445-15115 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T73","span":{"begin":115,"end":118},"obj":"Body_part"},{"id":"T74","span":{"begin":234,"end":237},"obj":"Body_part"},{"id":"T75","span":{"begin":630,"end":633},"obj":"Body_part"},{"id":"T76","span":{"begin":666,"end":669},"obj":"Body_part"}],"attributes":[{"id":"A73","pred":"fma_id","subj":"T73","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A74","pred":"fma_id","subj":"T74","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A76","pred":"fma_id","subj":"T76","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T44","span":{"begin":46,"end":50},"obj":"Disease"},{"id":"T45","span":{"begin":106,"end":114},"obj":"Disease"},{"id":"T46","span":{"begin":106,"end":110},"obj":"Disease"},{"id":"T47","span":{"begin":334,"end":342},"obj":"Disease"},{"id":"T48","span":{"begin":334,"end":338},"obj":"Disease"},{"id":"T49","span":{"begin":657,"end":665},"obj":"Disease"},{"id":"T50","span":{"begin":657,"end":661},"obj":"Disease"}],"attributes":[{"id":"A44","pred":"mondo_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A45","pred":"mondo_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A46","pred":"mondo_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A49","pred":"mondo_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A50","pred":"mondo_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T126","span":{"begin":134,"end":135},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T127","span":{"begin":300,"end":301},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T128","span":{"begin":562,"end":567},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T129","span":{"begin":599,"end":601},"obj":"http://purl.obolibrary.org/obo/CLO_0008307"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T44","span":{"begin":314,"end":319},"obj":"Chemical"},{"id":"T45","span":{"begin":375,"end":380},"obj":"Chemical"},{"id":"T46","span":{"begin":582,"end":587},"obj":"Chemical"},{"id":"T47","span":{"begin":599,"end":601},"obj":"Chemical"}],"attributes":[{"id":"A44","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A45","pred":"chebi_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A46","pred":"chebi_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A47","pred":"chebi_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/CHEBI_33472"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T12","span":{"begin":634,"end":644},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-sentences","denotations":[{"id":"T124","span":{"begin":0,"end":76},"obj":"Sentence"},{"id":"T125","span":{"begin":77,"end":413},"obj":"Sentence"},{"id":"T126","span":{"begin":414,"end":568},"obj":"Sentence"},{"id":"T127","span":{"begin":569,"end":670},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}

{"project":"LitCovid-PubTator","denotations":[{"id":"245","span":{"begin":66,"end":70},"obj":"Gene"},{"id":"246","span":{"begin":18,"end":40},"obj":"Species"},{"id":"247","span":{"begin":46,"end":62},"obj":"Species"},{"id":"256","span":{"begin":257,"end":261},"obj":"Gene"},{"id":"257","span":{"begin":106,"end":114},"obj":"Species"},{"id":"258","span":{"begin":224,"end":233},"obj":"Species"},{"id":"259","span":{"begin":334,"end":342},"obj":"Species"},{"id":"260","span":{"begin":347,"end":356},"obj":"Species"},{"id":"261","span":{"begin":403,"end":412},"obj":"Species"},{"id":"262","span":{"begin":620,"end":629},"obj":"Species"},{"id":"263","span":{"begin":657,"end":665},"obj":"Species"}],"attributes":[{"id":"A245","pred":"tao:has_database_id","subj":"245","obj":"Gene:43740578"},{"id":"A246","pred":"tao:has_database_id","subj":"246","obj":"Tax:2697049"},{"id":"A247","pred":"tao:has_database_id","subj":"247","obj":"Tax:694009"},{"id":"A256","pred":"tao:has_database_id","subj":"256","obj":"Gene:43740578"},{"id":"A257","pred":"tao:has_database_id","subj":"257","obj":"Tax:694009"},{"id":"A258","pred":"tao:has_database_id","subj":"258","obj":"Tax:2697049"},{"id":"A259","pred":"tao:has_database_id","subj":"259","obj":"Tax:694009"},{"id":"A260","pred":"tao:has_database_id","subj":"260","obj":"Tax:2697049"},{"id":"A261","pred":"tao:has_database_id","subj":"261","obj":"Tax:2697049"},{"id":"A262","pred":"tao:has_database_id","subj":"262","obj":"Tax:2697049"},{"id":"A263","pred":"tao:has_database_id","subj":"263","obj":"Tax:694009"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Discrimination of 2019 novel coronavirus from SARS coronavirus by RdRp assay\nFollowing the rationale that SARS-CoV RNA can be used as a positive control for the entire laboratory procedure, thus obviating the need to handle 2019-nCoV RNA, we formulated the RdRp assay so that it contains two probes: a broad-range probe reacting with SARS-CoV and 2019-nCoV and an additional probe that reacts only with 2019-nCoV. By limiting dilution experiments, we confirmed that both probes, whether used individually or in combination, provided the same LOD for each target virus. The specific probe RdRP_SARSr-P2 detected only the 2019-nCoV RNA transcript but not the SARS-CoV RNA."}