PMC:6988269 / 13622-14443 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6988269","sourcedb":"PMC","sourceid":"6988269","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6988269","text":"Sensitivity based on in vitro-transcribed RNA identical to 2019 novel coronavirus target sequences\nAlthough both assays detected 2019-nCoV without polymorphisms at oligonucleotide binding sites (Figure 2), we additionally generated in vitro-transcribed RNA standards that exactly matched the sequence of 2019-nCoV for absolute quantification and studying the limit of detection (LOD). Replicate reactions were done at concentrations around the detection end point determined in preliminary dilution experiments. The resulting LOD from replicate tests was 3.9 copies per reaction for the E gene assay and 3.6 copies per reaction for the RdRp assay (Figure 3C and D). These figures were close to the 95% hit rate of 2.9 copies per reaction, according to the Poisson distribution, expected when one RNA molecule is detected.","divisions":[{"label":"title","span":{"begin":0,"end":98}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T68","span":{"begin":42,"end":45},"obj":"Body_part"},{"id":"T69","span":{"begin":253,"end":256},"obj":"Body_part"},{"id":"T70","span":{"begin":589,"end":593},"obj":"Body_part"},{"id":"T71","span":{"begin":796,"end":808},"obj":"Body_part"},{"id":"T72","span":{"begin":796,"end":799},"obj":"Body_part"}],"attributes":[{"id":"A68","pred":"fma_id","subj":"T68","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A69","pred":"fma_id","subj":"T69","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A70","pred":"fma_id","subj":"T70","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A71","pred":"fma_id","subj":"T71","obj":"http://purl.org/sig/ont/fma/fma84126"},{"id":"A72","pred":"fma_id","subj":"T72","obj":"http://purl.org/sig/ont/fma/fma67095"},{"subj":"T68","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T69","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T70","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T71","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T124","span":{"begin":545,"end":550},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T125","span":{"begin":589,"end":593},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"}],"attributes":[{"subj":"T124","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T125","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T42","span":{"begin":164,"end":179},"obj":"Chemical"},{"id":"T43","span":{"begin":800,"end":808},"obj":"Chemical"}],"attributes":[{"id":"A42","pred":"chebi_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"},{"subj":"T42","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T43","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T119","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"T120","span":{"begin":99,"end":384},"obj":"Sentence"},{"id":"T121","span":{"begin":385,"end":511},"obj":"Sentence"},{"id":"T122","span":{"begin":512,"end":665},"obj":"Sentence"},{"id":"T123","span":{"begin":666,"end":821},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T119","pred":"source","obj":"LitCovid-sentences"},{"subj":"T120","pred":"source","obj":"LitCovid-sentences"},{"subj":"T121","pred":"source","obj":"LitCovid-sentences"},{"subj":"T122","pred":"source","obj":"LitCovid-sentences"},{"subj":"T123","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"233","span":{"begin":59,"end":81},"obj":"Species"},{"id":"238","span":{"begin":636,"end":640},"obj":"Gene"},{"id":"239","span":{"begin":129,"end":138},"obj":"Species"},{"id":"240","span":{"begin":304,"end":313},"obj":"Species"},{"id":"241","span":{"begin":164,"end":179},"obj":"Chemical"}],"attributes":[{"id":"A233","pred":"tao:has_database_id","subj":"233","obj":"Tax:2697049"},{"id":"A238","pred":"tao:has_database_id","subj":"238","obj":"Gene:43740578"},{"id":"A239","pred":"tao:has_database_id","subj":"239","obj":"Tax:2697049"},{"id":"A240","pred":"tao:has_database_id","subj":"240","obj":"Tax:2697049"},{"id":"A241","pred":"tao:has_database_id","subj":"241","obj":"MESH:D009841"},{"subj":"233","pred":"source","obj":"LitCovid-PubTator"},{"subj":"238","pred":"source","obj":"LitCovid-PubTator"},{"subj":"239","pred":"source","obj":"LitCovid-PubTator"},{"subj":"240","pred":"source","obj":"LitCovid-PubTator"},{"subj":"241","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#ec93ba","default":true},{"id":"LitCovid-PD-CLO","color":"#93d4ec"},{"id":"LitCovid-PD-CHEBI","color":"#ecea93"},{"id":"LitCovid-sentences","color":"#d093ec"},{"id":"LitCovid-PubTator","color":"#93ecb6"}]}]}}