PMC:6705340 / 11157-14829 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6705340","sourcedb":"PMC","sourceid":"6705340","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6705340","text":"Results\nThe statistical analysis showed that the weight of the restricted group decreased significantly (by 9%) in the first three days, and then increased (by 6.2% of the weight of the third day), which was conserved until the tenth day. Up to the 10th day there was a loss of approximately 4% of the initial weight. The weight of the sham group increased significantly from the first day to the tenth day, with an increase of 5.2% of the initial weight. The weight of the control group increased significantly from the first day to the tenth day, with an increase of 11% of the initial weight (Fig. 1).\nFigure 1 – The evolution of weight over 10 days in the normal, sham and restriction groups.\nIn the between-group comparisons of polymorphonuclear and lymphomononuclear cells, the polymorphonuclear cells and lymphocytes behaved similarly between the groups; there was a significant difference in the cell types, with pmn \u003e lymphocytes in all groups, and there was a significant difference between the groups independent of cell type (Fig. 2). In HE, it was found that after 10 days of gastric restriction, the height of the crypts in general decreased when compared to the sham and control animals (p\u003c0.05).\nFigure 2 – Polymorphonuclear and lymphomononuclear cell counts showing similarly between the groups; a significant difference in the cell types, with pmn \u003e lymphocytes in all groups (p\u003c0.001***).\nThe number of goblet cells showed no difference compared to the sham group (Figs. 3 and 7). There was a trend of maintaining trophism for 10 days observed in HE (Fig. 3), with an increase in neutral mucins and their cells and an increase in acid mucins (sulfomucins) and their respective crypts (Figs. 4 and 5). There were no differences in sialomucins or sulfomucins in the restricted group compared to the sham group, as well as height and the number of cells compared to the control group. We found a significant increase in total collagen in the restricted group compared to the sham and control groups (Figs. 6 and 7).\nFigure 3 – (A) Goblet cell counts, showing a difference between the groups; the restricted and sham groups have fewer goblet cells than the control group. (B) Crypt height, showing the decrease in height of the restrict group when compared to the sham and control animals (p\u003c0.05**).\nFigure 4 – (A) Semiquantification of neutral mucins; the restriction group has more neutral mucins than the other groups. (B) The difference in crypt height is only related to the control group; the goblet cells are similar to A (p\u003c0.05**).\nFigure 5 – (A) Semiquantification of AB acid mucins; the restriction group has more acid mucins than the other groups. (B) The difference in crypt height is only related to the restricted and sham groups; the goblet cells show that the restrict group is enhanced only in relation to the control group (p\u003c0.05**).\nFigure 7 – Goblet cells x400: (A-C) HE; (D-F) PAS; (G-H) AB; (J-L) Sirius Red. Not much difference can be observed between the crypts in A, B and C. There are more goblet cells in D and G. In J-L, the restrict group has more collagen than the control and sham groups.\nFigure 6 – Semiquantification of total collagen: collagen was enhanced in the restrict group compared to the control and sham groups (p\u003c0.05**).\nThere was no correlation of weight with the goblet cells stained by PAS, AB, or HID-AB (p = 0.051), and although not significant, these data indicate that for the height of the crypt controlled for PAS, the weight and height of the crypt increase.\nThere was a correlation between control weight and polymorphonuclear control cells; when polymorphonuclear cells increased, weight decreased.","divisions":[{"label":"title","span":{"begin":0,"end":7}},{"label":"p","span":{"begin":8,"end":604}},{"label":"p","span":{"begin":605,"end":696}},{"label":"figure","span":{"begin":605,"end":696}},{"label":"caption","span":{"begin":605,"end":696}},{"label":"title","span":{"begin":605,"end":696}},{"label":"p","span":{"begin":697,"end":1211}},{"label":"p","span":{"begin":1212,"end":1407}},{"label":"figure","span":{"begin":1212,"end":1407}},{"label":"caption","span":{"begin":1212,"end":1407}},{"label":"title","span":{"begin":1212,"end":1407}},{"label":"p","span":{"begin":1408,"end":2031}},{"label":"p","span":{"begin":2032,"end":2315}},{"label":"figure","span":{"begin":2032,"end":2315}},{"label":"caption","span":{"begin":2032,"end":2315}},{"label":"title","span":{"begin":2032,"end":2315}},{"label":"p","span":{"begin":2316,"end":2556}},{"label":"figure","span":{"begin":2316,"end":2556}},{"label":"label","span":{"begin":2316,"end":2324}},{"label":"caption","span":{"begin":2325,"end":2556}},{"label":"title","span":{"begin":2325,"end":2556}},{"label":"p","span":{"begin":2557,"end":2869}},{"label":"figure","span":{"begin":2557,"end":2869}},{"label":"caption","span":{"begin":2557,"end":2869}},{"label":"title","span":{"begin":2557,"end":2869}},{"label":"p","span":{"begin":2870,"end":3137}},{"label":"figure","span":{"begin":2870,"end":3137}},{"label":"caption","span":{"begin":2870,"end":3137}},{"label":"title","span":{"begin":2870,"end":3137}},{"label":"p","span":{"begin":3138,"end":3282}},{"label":"figure","span":{"begin":3138,"end":3282}},{"label":"caption","span":{"begin":3138,"end":3282}},{"label":"title","span":{"begin":3138,"end":3282}},{"label":"p","span":{"begin":3283,"end":3530}}],"tracks":[]}