PMC:6599329 / 29810-30520 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6599329","sourcedb":"PMC","sourceid":"6599329","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6599329","text":"For confocal imaging, an overnight culture of E. faecalis SK460 was diluted to OD600 = 0.1 and 4 ml of diluted broth was distributed to 6 well microtitre plate (Nunc, Roskilde, Denmark). A sterile square glass coverslip was inserted horizontally in each well and incubated at 37 °C in a static condition for two-time intervals (12 h, 24 h). Biofilm staining was carried out with Syto9 (Invitrogen, California, United States) as described previously [42]. The slides were then observed using a Nikon Eclipse Ti Confocal Laser scanning inverted microscope (Nikon Instruments Corporation, New York, United States). The measurement of biofilm thickness was performed using NIS-Element AR software, version 4.00.04.","tracks":[{"project":"2_test","denotations":[{"id":"31253082-16332862-12221389","span":{"begin":450,"end":452},"obj":"16332862"}],"attributes":[{"subj":"31253082-16332862-12221389","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93bb","default":true}]}]}}