PMC:6080766 / 7663-8074 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6080766","sourcedb":"PMC","sourceid":"6080766","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6080766","text":"Cell Culture\nDermal fibroblasts were obtained by skin-punch biopsy and were maintained in amnioMAX C-100 complete medium (Life Technologies) in a 37°C incubator with 5% CO2 and 3% O2. siRNA oligonucleotides (siLUC: 5′-CUUACGCUGAGUACUUCGA-3′ [siTopIIIα SMARTpool M-005279-01-0005, Dharmacon]) were transfected into dermal fibroblasts with RNAiMAX (Life Technologies) according to the manufacturer’s instructions.","divisions":[{"label":"Title","span":{"begin":0,"end":12}}],"tracks":[]}