Cell Culture
Dermal fibroblasts were obtained by skin-punch biopsy and were maintained in amnioMAX C-100 complete medium (Life Technologies) in a 37°C incubator with 5% CO2 and 3% O2. siRNA oligonucleotides (siLUC: 5′-CUUACGCUGAGUACUUCGA-3′ [siTopIIIα SMARTpool M-005279-01-0005, Dharmacon]) were transfected into dermal fibroblasts with RNAiMAX (Life Technologies) according to the manufacturer’s instructions.