PMC:5985359 / 17521-17898
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5985359","sourcedb":"PMC","sourceid":"5985359","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5985359","text":"Immunohistochemistry\nEyes were enucleated and immersion fixed in 4% PFA for 24 hr prior to rapid freezing in OCT imbedding compound. Eyes were removed and cryoprotected by overnight incubation (at 4°C) in 30% sucrose solution. Corneal tissue sections (16 μm) were cut on a cryostat and collected onto charged slides and viewed immediately on a confocal fluorescence microscope.","divisions":[{"label":"title","span":{"begin":0,"end":20}}],"tracks":[]}