PMC:5944998 / 37589-40875 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5944998","sourcedb":"PMC","sourceid":"5944998","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5944998","text":"FvJAZ proteins show conserved TIFY and Jas domains\nTo gain further insights in the primary protein structure and evolutionary relationships in JAZ and MYC protein families, multiple sequence alignment and phylogenetic analyses were performed (Figs 3–6, S2 and S3 Figs). FvJAZ proteins exhibited similar length and conserved structure according to that observed in Arabidopsis (Table 1) and previously reported in V. vinifera [51]. Analysis of the deduced amino acid sequence of F. vesca JAZs showed the conservation of the TIFY/ZIM domain that characterize this family (Figs 4A and 5A) [18,21] and a Jas domain (Figs 4B and 5B), which is specific of JAZ subfamily [10,20,21]. In F. vesca, the TIFY domain contains the highly conserved TIFY[F/Y]XG motif [18] (Figs 4A and 5A) as consensus sequence, however, some alternative sequences like TVFYXG and TIFFXG were found in FvJAZ10 and FvJAZ11, respectively (Fig 4A), as previously described for TIFY proteins in other species [21]. Jas domain maintained the conserved central amino acidic residues SLX2FLXKR[K/R]X[R/E] according to consensus sequence observed in F. vesca (Figs 4B and 5B) and similar to the previously reported SLX2FX2KRX2R sequence in Arabidopsis [20]. Besides, FvJAZ7, FvJAZ8.1 and FvJAZ8.2 displayed a variant Jas sequence (Fig 4B and S3 Fig) similar to that observed in Arabidopsis for JAZ7 and JAZ8 proteins [66]. Most of Arabidopsis JAZs contain a degron sequence at the Jas domain that is necessary for interaction with COI1 and JA-Ile [12], but only FvJAZ10 showed the canonical degron LPIAR(R/K) previously described for AtJAZ1, AtJAZ2, AtJAZ10 and AtJAZ12 proteins [66]. Other FvJAZ proteins displayed variations in degron sequences such as IPMQRK in FvJAZ1 (Fig 4B). Moreover, the degron sequence displayed the conserved residues R(K), and S and R (Fig 4B) interacting with COI1 [12] and MYC3 [67], respectively. Therefore, NLS located in C-terminal region [68] showed amino acidic residues conserved among JAZ proteins of F. vesca (Figs 4B and 5B). In some Arabidopsis JAZs, the N-terminal LxLxL type of EAR motif allows recruitment of TPL co-repressors to repress JA signalling pathway through a NINJA independent molecular mechanism [66]. In this regard, FvJAZ5 displayed the LxLxL type of EAR-motif at C-terminus, and FvJAZ7, FvJAZ8.1 and FvJAZ8.2 presented it at the N-terminus (S2 Fig) similar to that reported for AtJAZ5 and AtJAZ6, and AtJAZ7 and AtJAZ8 proteins, respectively (Fig 3) [66,69]. FvJAZ5 showed the lack of DLNEPT type of EAR-motif (Panel A in S2 Fig) in N-terminal region previously described in AtJAZ5 and AtJAZ6 proteins [66,69]. Phylogenetic analysis displayed evolutionary relationships between F. vesca, A. thaliana, M. × domestica, V. vinifera, S. lycopersicum and O. sativa JAZ proteins (Fig 6A). Protein grouping of FvJAZ proteins are related with high sequence identity respect to AtJAZ proteins (Fig 3A and S4 Table). The FvJAZ proteins clustered together with their Arabidopsis orthologs in a similar fashion than observed in several plant species [45,48,50,51], however, some phylogenetic trees contain lower bootstrap values [51]. At the same time, protein positions of FvJAZs in tree groups (Fig 6A) is in agreement with the identity and synteny analyses (Fig 1 and S1 Fig).","divisions":[{"label":"Title","span":{"begin":0,"end":50}}],"tracks":[{"project":"2_test","denotations":[{"id":"29746533-22984514-90904130","span":{"begin":426,"end":428},"obj":"22984514"},{"id":"29746533-17499004-90904131","span":{"begin":587,"end":589},"obj":"17499004"},{"id":"29746533-21616136-90904132","span":{"begin":590,"end":592},"obj":"21616136"},{"id":"29746533-27490895-90904133","span":{"begin":665,"end":667},"obj":"27490895"},{"id":"29746533-21963667-90904134","span":{"begin":668,"end":670},"obj":"21963667"},{"id":"29746533-21616136-90904135","span":{"begin":671,"end":673},"obj":"21616136"},{"id":"29746533-17499004-90904136","span":{"begin":754,"end":756},"obj":"17499004"},{"id":"29746533-21616136-90904137","span":{"begin":975,"end":977},"obj":"21616136"},{"id":"29746533-21963667-90904138","span":{"begin":1214,"end":1216},"obj":"21963667"},{"id":"29746533-22327740-90904139","span":{"begin":1379,"end":1381},"obj":"22327740"},{"id":"29746533-20927106-90904140","span":{"begin":1509,"end":1511},"obj":"20927106"},{"id":"29746533-22327740-90904141","span":{"begin":1641,"end":1643},"obj":"22327740"},{"id":"29746533-20927106-90904142","span":{"begin":1856,"end":1858},"obj":"20927106"},{"id":"29746533-26258305-90904143","span":{"begin":1870,"end":1872},"obj":"26258305"},{"id":"29746533-19575013-90904144","span":{"begin":1934,"end":1936},"obj":"19575013"},{"id":"29746533-22327740-90904145","span":{"begin":2213,"end":2215},"obj":"22327740"},{"id":"29746533-22327740-90904146","span":{"begin":2470,"end":2472},"obj":"22327740"},{"id":"29746533-26896849-90904147","span":{"begin":2473,"end":2475},"obj":"26896849"},{"id":"29746533-22327740-90904148","span":{"begin":2622,"end":2624},"obj":"22327740"},{"id":"29746533-26896849-90904149","span":{"begin":2625,"end":2627},"obj":"26896849"},{"id":"29746533-19618278-90904150","span":{"begin":3058,"end":3060},"obj":"19618278"},{"id":"29746533-28570564-90904151","span":{"begin":3061,"end":3063},"obj":"28570564"},{"id":"29746533-22984514-90904152","span":{"begin":3067,"end":3069},"obj":"22984514"},{"id":"29746533-22984514-90904153","span":{"begin":3137,"end":3139},"obj":"22984514"}],"attributes":[{"subj":"29746533-22984514-90904130","pred":"source","obj":"2_test"},{"subj":"29746533-17499004-90904131","pred":"source","obj":"2_test"},{"subj":"29746533-21616136-90904132","pred":"source","obj":"2_test"},{"subj":"29746533-27490895-90904133","pred":"source","obj":"2_test"},{"subj":"29746533-21963667-90904134","pred":"source","obj":"2_test"},{"subj":"29746533-21616136-90904135","pred":"source","obj":"2_test"},{"subj":"29746533-17499004-90904136","pred":"source","obj":"2_test"},{"subj":"29746533-21616136-90904137","pred":"source","obj":"2_test"},{"subj":"29746533-21963667-90904138","pred":"source","obj":"2_test"},{"subj":"29746533-22327740-90904139","pred":"source","obj":"2_test"},{"subj":"29746533-20927106-90904140","pred":"source","obj":"2_test"},{"subj":"29746533-22327740-90904141","pred":"source","obj":"2_test"},{"subj":"29746533-20927106-90904142","pred":"source","obj":"2_test"},{"subj":"29746533-26258305-90904143","pred":"source","obj":"2_test"},{"subj":"29746533-19575013-90904144","pred":"source","obj":"2_test"},{"subj":"29746533-22327740-90904145","pred":"source","obj":"2_test"},{"subj":"29746533-22327740-90904146","pred":"source","obj":"2_test"},{"subj":"29746533-26896849-90904147","pred":"source","obj":"2_test"},{"subj":"29746533-22327740-90904148","pred":"source","obj":"2_test"},{"subj":"29746533-26896849-90904149","pred":"source","obj":"2_test"},{"subj":"29746533-19618278-90904150","pred":"source","obj":"2_test"},{"subj":"29746533-28570564-90904151","pred":"source","obj":"2_test"},{"subj":"29746533-22984514-90904152","pred":"source","obj":"2_test"},{"subj":"29746533-22984514-90904153","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#bfec93","default":true}]}]}}