PMC:5944998 / 12481-13522 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5944998","sourcedb":"PMC","sourceid":"5944998","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5944998","text":"Determination of anthocyanins and proanthocyanidins (PAs) contents\nFive grams of frozen fruit skin tissue without achenes were grounded with liquid nitrogen, homogenized with 15 ml of acetone/water (80/20 v/v) and stored at -20°C until use. Anthocyanins quantification was performed according to previously reported [42]. Briefly, 50 μl of fruit extract and 150 μl of corresponding buffer were dispensed into a 96-well plate. The absorbance was measured at 509 nm and 700 nm, considering ε = 17,330 L/cm1.mol1. The results were expressed as mg of pelargonidin-3-glucoside equivalents per 100 g of fresh weight (FW). PAs content was measured according to previously reported [43]. Briefly, 70 μl of fruit extract diluted (1/10, v/v) and 210 μl of dimethylaminocinnamaldehyde (DMAC) reagent were dispensed into wells of a 96-well plate. The microplate was read for 20 min at 640 nm. The concentration was calculated from a calibration curve, using catechin as standard. The results were expressed as mg of catechin equivalents per 100 g of FW.","divisions":[{"label":"Title","span":{"begin":0,"end":66}}],"tracks":[{"project":"2_test","denotations":[{"id":"29746533-20549799-90904100","span":{"begin":675,"end":677},"obj":"20549799"}],"attributes":[{"subj":"29746533-20549799-90904100","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93daec","default":true}]}]}}