PMC:5711939 / 28805-29475 JSONTXT

{"project":"2_test","denotations":[{"id":"29214045-18274517-76397138","span":{"begin":85,"end":87},"obj":"18274517"},{"id":"29214045-11420333-76397139","span":{"begin":405,"end":407},"obj":"11420333"}],"text":"Determination of MIC\nMICs were determined using a modified broth microdilution method36 in cation adjusted Mueller Hinton broth (MHB) following the International Organization for Standardization 20776-1 standard for MIC testing with a final bacterial inoculum concentration of 5 × 105 CFU/ml.37 Peptides dissolved in sterile distilled water and comparator antibiotics dissolved in the appropriate solvents38 were added to sterile U-bottom polypropylene 96-well microplates at desired concentrations. MIC was defined as the lowest concentration of peptide or antibiotic, which inhibited the visible growth of bacteria after 18–24 h incubation at appropriate temperatures."}