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addition to its suggested importance for phagocytosis of apoptotic cells, it has been proposed that Ptdsr fulfils a second crucial role in regulating and maintaining a non-inflammatory environment upon the recognition of apoptotic cells by macrophages [26]. We therefore tested whether Ptdsr -/- macrophages were able to release anti-inflammatory cytokines after ingestion of apoptotic cells. We examined levels of TGF-β1 and interleukin-10 (IL-10) after stimulation of FLDMs with lipopolysaccharide (LPS), with and without co-culture of apoptotic cells. Quantification of TGF-β1 and IL-10 levels after 22 hours of culture demonstrated that Ptdsr -/- macrophages were able to secrete these anti-inflammatory cytokines upon ingestion of apoptotic cells, although at a slightly lower level than wild-type (Figure 8a,b). This indicates that ablation of Ptdsr function does not compromise in general the ability of macrophages to release immune-suppressive cytokines after recognition and engulfment of apoptotic cells."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T8196","span":{"begin":44,"end":75},"obj":"GO:0043277"},{"id":"T8197","span":{"begin":60,"end":75},"obj":"CL:0000445"},{"id":"T8198","span":{"begin":70,"end":75},"obj":"CL_GO_EXT:cell"},{"id":"T8199","span":{"begin":103,"end":108},"obj":"PR_EXT:000009218"},{"id":"T8200","span":{"begin":142,"end":152},"obj":"GO:0065007"},{"id":"T8201","span":{"begin":175,"end":187},"obj":"GO_PATO_EXT:inflammatory_process_or_quality"},{"id":"T8202","span":{"begin":224,"end":233},"obj":"GO_PATO_EXT:apoptotic_process_or_quality"},{"id":"T8203","span":{"begin":224,"end":239},"obj":"CL:0000445"},{"id":"T8204","span":{"begin":234,"end":239},"obj":"CL_GO_EXT:cell"},{"id":"T8205","span":{"begin":243,"end":254},"obj":"CL:0000235"},{"id":"T8206","span":{"begin":289,"end":294},"obj":"PR_EXT:000009218"},{"id":"T8207","span":{"begin":295,"end":296},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T8208","span":{"begin":297,"end":298},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T8209","span":{"begin":299,"end":310},"obj":"CL:0000235"},{"id":"T8210","span":{"begin":324,"end":331},"obj":"GO_EXT:biological_release"},{"id":"T8211","span":{"begin":337,"end":349},"obj":"GO_PATO_EXT:inflammatory_process_or_quality"},{"id":"T8212","span":{"begin":350,"end":359},"obj":"GO_EXT:0005125"},{"id":"T8213","span":{"begin":366,"end":394},"obj":"GO:0043652"},{"id":"T8214","span":{"begin":379,"end":394},"obj":"CL:0000445"},{"id":"T8215","span":{"begin":389,"end":394},"obj":"CL_GO_EXT:cell"},{"id":"T8216","span":{"begin":418,"end":424},"obj":"PR_EXT:000000182"},{"id":"T8217","span":{"begin":429,"end":443},"obj":"PR_EXT:000001471"},{"id":"T8218","span":{"begin":445,"end":450},"obj":"PR_EXT:000001471"},{"id":"T8219","span":{"begin":484,"end":502},"obj":"CHEBI:16412"},{"id":"T8220","span":{"begin":504,"end":507},"obj":"CHEBI:16412"},{"id":"T8221","span":{"begin":541,"end":550},"obj":"GO_PATO_EXT:apoptotic_process_or_quality"},{"id":"T8222","span":{"begin":541,"end":556},"obj":"CL:0000445"},{"id":"T8223","span":{"begin":551,"end":556},"obj":"CL_GO_EXT:cell"},{"id":"T8224","span":{"begin":576,"end":582},"obj":"PR_EXT:000000182"},{"id":"T8225","span":{"begin":587,"end":592},"obj":"PR_EXT:000001471"},{"id":"T8226","span":{"begin":644,"end":649},"obj":"PR_EXT:000009218"},{"id":"T8227","span":{"begin":650,"end":651},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T8228","span":{"begin":652,"end":653},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T8229","span":{"begin":654,"end":665},"obj":"CL:0000235"},{"id":"T8230","span":{"begin":679,"end":686},"obj":"GO:0046903"},{"id":"T8231","span":{"begin":698,"end":710},"obj":"GO_PATO_EXT:inflammatory_process_or_quality"},{"id":"T8232","span":{"begin":711,"end":720},"obj":"GO_EXT:0005125"},{"id":"T8233","span":{"begin":726,"end":754},"obj":"GO:0043652"},{"id":"T8234","span":{"begin":739,"end":754},"obj":"CL:0000445"},{"id":"T8235","span":{"begin":749,"end":754},"obj":"CL_GO_EXT:cell"},{"id":"T8236","span":{"begin":796,"end":805},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T8237","span":{"begin":853,"end":858},"obj":"PR_EXT:000009218"},{"id":"T8238","span":{"begin":914,"end":925},"obj":"CL:0000235"},{"id":"T8239","span":{"begin":929,"end":936},"obj":"GO_EXT:biological_release"},{"id":"T8240","span":{"begin":937,"end":943},"obj":"UBERON:0002405"},{"id":"T8241","span":{"begin":956,"end":965},"obj":"GO_EXT:0005125"},{"id":"T8242","span":{"begin":988,"end":1017},"obj":"GO:0043652"},{"id":"T8243","span":{"begin":1002,"end":1017},"obj":"CL:0000445"},{"id":"T8244","span":{"begin":1012,"end":1017},"obj":"CL_GO_EXT:cell"}],"text":"In addition to its suggested importance for phagocytosis of apoptotic cells, it has been proposed that Ptdsr fulfils a second crucial role in regulating and maintaining a non-inflammatory environment upon the recognition of apoptotic cells by macrophages [26]. We therefore tested whether Ptdsr -/- macrophages were able to release anti-inflammatory cytokines after ingestion of apoptotic cells. We examined levels of TGF-β1 and interleukin-10 (IL-10) after stimulation of FLDMs with lipopolysaccharide (LPS), with and without co-culture of apoptotic cells. Quantification of TGF-β1 and IL-10 levels after 22 hours of culture demonstrated that Ptdsr -/- macrophages were able to secrete these anti-inflammatory cytokines upon ingestion of apoptotic cells, although at a slightly lower level than wild-type (Figure 8a,b). This indicates that ablation of Ptdsr function does not compromise in general the ability of macrophages to release immune-suppressive cytokines after recognition and engulfment of apoptotic cells."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T8106","span":{"begin":44,"end":75},"obj":"GO:0043277"},{"id":"T8107","span":{"begin":60,"end":75},"obj":"CL:0000445"},{"id":"T8108","span":{"begin":103,"end":108},"obj":"PR:000009218"},{"id":"T8109","span":{"begin":142,"end":152},"obj":"GO:0065007"},{"id":"T8110","span":{"begin":224,"end":239},"obj":"CL:0000445"},{"id":"T8111","span":{"begin":243,"end":254},"obj":"CL:0000235"},{"id":"T8112","span":{"begin":289,"end":294},"obj":"PR:000009218"},{"id":"T8113","span":{"begin":299,"end":310},"obj":"CL:0000235"},{"id":"T8114","span":{"begin":366,"end":394},"obj":"GO:0043652"},{"id":"T8115","span":{"begin":379,"end":394},"obj":"CL:0000445"},{"id":"T8116","span":{"begin":418,"end":424},"obj":"PR:000000182"},{"id":"T8117","span":{"begin":429,"end":443},"obj":"PR:000001471"},{"id":"T8118","span":{"begin":445,"end":450},"obj":"PR:000001471"},{"id":"T8119","span":{"begin":484,"end":502},"obj":"CHEBI:16412"},{"id":"T8120","span":{"begin":504,"end":507},"obj":"CHEBI:16412"},{"id":"T8121","span":{"begin":541,"end":556},"obj":"CL:0000445"},{"id":"T8122","span":{"begin":576,"end":582},"obj":"PR:000000182"},{"id":"T8123","span":{"begin":587,"end":592},"obj":"PR:000001471"},{"id":"T8124","span":{"begin":644,"end":649},"obj":"PR:000009218"},{"id":"T8125","span":{"begin":654,"end":665},"obj":"CL:0000235"},{"id":"T8126","span":{"begin":679,"end":686},"obj":"GO:0046903"},{"id":"T8127","span":{"begin":726,"end":754},"obj":"GO:0043652"},{"id":"T8128","span":{"begin":739,"end":754},"obj":"CL:0000445"},{"id":"T8129","span":{"begin":853,"end":858},"obj":"PR:000009218"},{"id":"T8130","span":{"begin":914,"end":925},"obj":"CL:0000235"},{"id":"T8131","span":{"begin":937,"end":943},"obj":"UBERON:0002405"},{"id":"T8132","span":{"begin":988,"end":1017},"obj":"GO:0043652"},{"id":"T8133","span":{"begin":1002,"end":1017},"obj":"CL:0000445"}],"text":"In addition to its suggested importance for phagocytosis of apoptotic cells, it has been proposed that Ptdsr fulfils a second crucial role in regulating and maintaining a non-inflammatory environment upon the recognition of apoptotic cells by macrophages [26]. We therefore tested whether Ptdsr -/- macrophages were able to release anti-inflammatory cytokines after ingestion of apoptotic cells. We examined levels of TGF-β1 and interleukin-10 (IL-10) after stimulation of FLDMs with lipopolysaccharide (LPS), with and without co-culture of apoptotic cells. Quantification of TGF-β1 and IL-10 levels after 22 hours of culture demonstrated that Ptdsr -/- macrophages were able to secrete these anti-inflammatory cytokines upon ingestion of apoptotic cells, although at a slightly lower level than wild-type (Figure 8a,b). This indicates that ablation of Ptdsr function does not compromise in general the ability of macrophages to release immune-suppressive cytokines after recognition and engulfment of apoptotic cells."}

{"project":"2_test","denotations":[{"id":"15345036-10811223-55938511","span":{"begin":256,"end":258},"obj":"10811223"}],"text":"In addition to its suggested importance for phagocytosis of apoptotic cells, it has been proposed that Ptdsr fulfils a second crucial role in regulating and maintaining a non-inflammatory environment upon the recognition of apoptotic cells by macrophages [26]. We therefore tested whether Ptdsr -/- macrophages were able to release anti-inflammatory cytokines after ingestion of apoptotic cells. We examined levels of TGF-β1 and interleukin-10 (IL-10) after stimulation of FLDMs with lipopolysaccharide (LPS), with and without co-culture of apoptotic cells. Quantification of TGF-β1 and IL-10 levels after 22 hours of culture demonstrated that Ptdsr -/- macrophages were able to secrete these anti-inflammatory cytokines upon ingestion of apoptotic cells, although at a slightly lower level than wild-type (Figure 8a,b). This indicates that ablation of Ptdsr function does not compromise in general the ability of macrophages to release immune-suppressive cytokines after recognition and engulfment of apoptotic cells."}