PMC:539297 / 32772-33685 JSONTXT

Annnotations TAB JSON ListView MergeView

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T7097","span":{"begin":7,"end":13},"obj":"UBERON:0000479"},{"id":"T7098","span":{"begin":182,"end":186},"obj":"CHEBI:9754"},{"id":"T7099","span":{"begin":201,"end":213},"obj":"CHEBI:9750"},{"id":"T7100","span":{"begin":475,"end":486},"obj":"PR:000006025"},{"id":"T7101","span":{"begin":506,"end":517},"obj":"CHEBI:76219"},{"id":"T7102","span":{"begin":528,"end":539},"obj":"PR:000006025"}],"text":"100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes. The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay. 50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations. Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time."}

    craft-sa-dev

    {"project":"craft-sa-dev","denotations":[{"id":"T7121","span":{"begin":0,"end":3},"obj":"CD"},{"id":"T7122","span":{"begin":4,"end":6},"obj":"NN"},{"id":"T7123","span":{"begin":22,"end":28},"obj":"NNS"},{"id":"T7124","span":{"begin":7,"end":13},"obj":"NN"},{"id":"T7125","span":{"begin":14,"end":21},"obj":"NN"},{"id":"T7126","span":{"begin":78,"end":84},"obj":"VBN"},{"id":"T7127","span":{"begin":28,"end":30},"obj":","},{"id":"T7128","span":{"begin":30,"end":35},"obj":"WDT"},{"id":"T7129","span":{"begin":36,"end":40},"obj":"VBD"},{"id":"T7130","span":{"begin":41,"end":54},"obj":"RB"},{"id":"T7131","span":{"begin":58,"end":60},"obj":"CD"},{"id":"T7132","span":{"begin":55,"end":57},"obj":"CD"},{"id":"T7133","span":{"begin":57,"end":58},"obj":"SYM"},{"id":"T7134","span":{"begin":60,"end":61},"obj":"NN"},{"id":"T7135","span":{"begin":62,"end":71},"obj":"JJ"},{"id":"T7136","span":{"begin":71,"end":73},"obj":","},{"id":"T7137","span":{"begin":73,"end":77},"obj":"VBD"},{"id":"T7138","span":{"begin":85,"end":92},"obj":"RB"},{"id":"T7139","span":{"begin":93,"end":97},"obj":"IN"},{"id":"T7140","span":{"begin":98,"end":101},"obj":"NN"},{"id":"T7141","span":{"begin":102,"end":106},"obj":"JJ"},{"id":"T7142","span":{"begin":101,"end":102},"obj":"HYPH"},{"id":"T7143","span":{"begin":107,"end":110},"obj":"NN"},{"id":"T7144","span":{"begin":110,"end":112},"obj":","},{"id":"T7145","span":{"begin":112,"end":115},"obj":"CC"},{"id":"T7146","span":{"begin":116,"end":121},"obj":"JJ"},{"id":"T7147","span":{"begin":130,"end":138},"obj":"NNS"},{"id":"T7148","span":{"begin":122,"end":129},"obj":"NN"},{"id":"T7149","span":{"begin":144,"end":152},"obj":"VBN"},{"id":"T7150","span":{"begin":139,"end":143},"obj":"VBD"},{"id":"T7151","span":{"begin":153,"end":155},"obj":"IN"},{"id":"T7152","span":{"begin":156,"end":164},"obj":"VBG"},{"id":"T7153","span":{"begin":165,"end":170},"obj":"NNS"},{"id":"T7154","span":{"begin":171,"end":175},"obj":"IN"},{"id":"T7155","span":{"begin":176,"end":178},"obj":"CD"},{"id":"T7156","span":{"begin":179,"end":181},"obj":"NN"},{"id":"T7157","span":{"begin":182,"end":186},"obj":"NN"},{"id":"T7158","span":{"begin":186,"end":188},"obj":","},{"id":"T7159","span":{"begin":188,"end":190},"obj":"NN"},{"id":"T7160","span":{"begin":191,"end":194},"obj":"CD"},{"id":"T7161","span":{"begin":194,"end":196},"obj":","},{"id":"T7162","span":{"begin":196,"end":199},"obj":"CD"},{"id":"T7163","span":{"begin":199,"end":200},"obj":"NN"},{"id":"T7164","span":{"begin":208,"end":209},"obj":"NN"},{"id":"T7165","span":{"begin":201,"end":207},"obj":"NN"},{"id":"T7166","span":{"begin":209,"end":210},"obj":"HYPH"},{"id":"T7167","span":{"begin":210,"end":213},"obj":"CD"},{"id":"T7168","span":{"begin":214,"end":222},"obj":"VBN"},{"id":"T7169","span":{"begin":223,"end":225},"obj":"IN"},{"id":"T7170","span":{"begin":226,"end":236},"obj":"NN"},{"id":"T7171","span":{"begin":237,"end":239},"obj":"IN"},{"id":"T7172","span":{"begin":240,"end":243},"obj":"NN"},{"id":"T7173","span":{"begin":243,"end":245},"obj":","},{"id":"T7174","span":{"begin":245,"end":248},"obj":"IN"},{"id":"T7175","span":{"begin":249,"end":251},"obj":"CD"},{"id":"T7176","span":{"begin":252,"end":259},"obj":"NNS"},{"id":"T7177","span":{"begin":259,"end":260},"obj":"."},{"id":"T7178","span":{"begin":260,"end":419},"obj":"sentence"},{"id":"T7179","span":{"begin":261,"end":264},"obj":"DT"},{"id":"T7180","span":{"begin":275,"end":283},"obj":"NN"},{"id":"T7181","span":{"begin":265,"end":274},"obj":"JJ"},{"id":"T7182","span":{"begin":288,"end":299},"obj":"VBN"},{"id":"T7183","span":{"begin":284,"end":287},"obj":"VBD"},{"id":"T7184","span":{"begin":300,"end":302},"obj":"IN"},{"id":"T7185","span":{"begin":303,"end":309},"obj":"CD"},{"id":"T7186","span":{"begin":310,"end":311},"obj":"NN"},{"id":"T7187","span":{"begin":311,"end":313},"obj":","},{"id":"T7188","span":{"begin":313,"end":316},"obj":"DT"},{"id":"T7189","span":{"begin":317,"end":328},"obj":"NN"},{"id":"T7190","span":{"begin":333,"end":341},"obj":"VBN"},{"id":"T7191","span":{"begin":329,"end":332},"obj":"VBD"},{"id":"T7192","span":{"begin":341,"end":343},"obj":","},{"id":"T7193","span":{"begin":343,"end":346},"obj":"CC"},{"id":"T7194","span":{"begin":347,"end":354},"obj":"NN"},{"id":"T7195","span":{"begin":355,"end":368},"obj":"NN"},{"id":"T7196","span":{"begin":373,"end":383},"obj":"VBN"},{"id":"T7197","span":{"begin":369,"end":372},"obj":"VBD"},{"id":"T7198","span":{"begin":384,"end":389},"obj":"VBG"},{"id":"T7199","span":{"begin":390,"end":393},"obj":"DT"},{"id":"T7200","span":{"begin":413,"end":418},"obj":"NN"},{"id":"T7201","span":{"begin":394,"end":397},"obj":"NNP"},{"id":"T7202","span":{"begin":398,"end":401},"obj":"NNP"},{"id":"T7203","span":{"begin":397,"end":398},"obj":"HYPH"},{"id":"T7204","span":{"begin":402,"end":404},"obj":"NN"},{"id":"T7205","span":{"begin":405,"end":412},"obj":"NN"},{"id":"T7206","span":{"begin":418,"end":419},"obj":"."},{"id":"T7207","span":{"begin":419,"end":639},"obj":"sentence"},{"id":"T7208","span":{"begin":420,"end":422},"obj":"CD"},{"id":"T7209","span":{"begin":423,"end":425},"obj":"CD"},{"id":"T7210","span":{"begin":422,"end":423},"obj":"SYM"},{"id":"T7211","span":{"begin":426,"end":428},"obj":"NN"},{"id":"T7212","span":{"begin":459,"end":463},"obj":"VBN"},{"id":"T7213","span":{"begin":429,"end":431},"obj":"IN"},{"id":"T7214","span":{"begin":432,"end":437},"obj":"JJ"},{"id":"T7215","span":{"begin":446,"end":453},"obj":"NN"},{"id":"T7216","span":{"begin":438,"end":445},"obj":"NN"},{"id":"T7217","span":{"begin":454,"end":458},"obj":"VBD"},{"id":"T7218","span":{"begin":464,"end":466},"obj":"TO"},{"id":"T7219","span":{"begin":467,"end":474},"obj":"VB"},{"id":"T7220","span":{"begin":475,"end":484},"obj":"NN"},{"id":"T7221","span":{"begin":485,"end":486},"obj":"NN"},{"id":"T7222","span":{"begin":487,"end":495},"obj":"NN"},{"id":"T7223","span":{"begin":496,"end":501},"obj":"VBG"},{"id":"T7224","span":{"begin":502,"end":505},"obj":"DT"},{"id":"T7225","span":{"begin":569,"end":572},"obj":"NN"},{"id":"T7226","span":{"begin":506,"end":517},"obj":"JJ"},{"id":"T7227","span":{"begin":538,"end":539},"obj":"NN"},{"id":"T7228","span":{"begin":518,"end":526},"obj":"NNP"},{"id":"T7229","span":{"begin":526,"end":527},"obj":"SYM"},{"id":"T7230","span":{"begin":528,"end":537},"obj":"NN"},{"id":"T7231","span":{"begin":540,"end":553},"obj":"NN"},{"id":"T7232","span":{"begin":554,"end":562},"obj":"NN"},{"id":"T7233","span":{"begin":563,"end":568},"obj":"NN"},{"id":"T7234","span":{"begin":573,"end":574},"obj":"-LRB-"},{"id":"T7235","span":{"begin":578,"end":589},"obj":"NNP"},{"id":"T7236","span":{"begin":574,"end":577},"obj":"NNP"},{"id":"T7237","span":{"begin":589,"end":590},"obj":"-RRB-"},{"id":"T7238","span":{"begin":591,"end":600},"obj":"VBG"},{"id":"T7239","span":{"begin":601,"end":603},"obj":"IN"},{"id":"T7240","span":{"begin":604,"end":607},"obj":"DT"},{"id":"T7241","span":{"begin":608,"end":620},"obj":"NN"},{"id":"T7242","span":{"begin":623,"end":638},"obj":"NNS"},{"id":"T7243","span":{"begin":620,"end":622},"obj":"POS"},{"id":"T7244","span":{"begin":638,"end":639},"obj":"."},{"id":"T7245","span":{"begin":639,"end":913},"obj":"sentence"},{"id":"T7246","span":{"begin":640,"end":648},"obj":"JJ"},{"id":"T7247","span":{"begin":649,"end":661},"obj":"NN"},{"id":"T7248","span":{"begin":666,"end":674},"obj":"VBN"},{"id":"T7249","span":{"begin":662,"end":665},"obj":"VBD"},{"id":"T7250","span":{"begin":675,"end":680},"obj":"VBG"},{"id":"T7251","span":{"begin":681,"end":683},"obj":"DT"},{"id":"T7252","span":{"begin":701,"end":707},"obj":"NN"},{"id":"T7253","span":{"begin":684,"end":691},"obj":"NN"},{"id":"T7254","span":{"begin":692,"end":694},"obj":"NN"},{"id":"T7255","span":{"begin":695,"end":700},"obj":"NN"},{"id":"T7256","span":{"begin":708,"end":709},"obj":"-LRB-"},{"id":"T7257","span":{"begin":719,"end":726},"obj":"NNPS"},{"id":"T7258","span":{"begin":709,"end":718},"obj":"JJ"},{"id":"T7259","span":{"begin":726,"end":727},"obj":"-RRB-"},{"id":"T7260","span":{"begin":728,"end":732},"obj":"IN"},{"id":"T7261","span":{"begin":733,"end":736},"obj":"DT"},{"id":"T7262","span":{"begin":747,"end":754},"obj":"NNS"},{"id":"T7263","span":{"begin":737,"end":746},"obj":"JJ"},{"id":"T7264","span":{"begin":755,"end":758},"obj":"CC"},{"id":"T7265","span":{"begin":759,"end":767},"obj":"NNS"},{"id":"T7266","span":{"begin":767,"end":769},"obj":":"},{"id":"T7267","span":{"begin":769,"end":779},"obj":"NN"},{"id":"T7268","span":{"begin":780,"end":786},"obj":"NN"},{"id":"T7269","span":{"begin":792,"end":794},"obj":"CD"},{"id":"T7270","span":{"begin":786,"end":788},"obj":","},{"id":"T7271","span":{"begin":788,"end":791},"obj":"CD"},{"id":"T7272","span":{"begin":791,"end":792},"obj":"HYPH"},{"id":"T7273","span":{"begin":794,"end":795},"obj":":"},{"id":"T7274","span":{"begin":796,"end":804},"obj":"NN"},{"id":"T7275","span":{"begin":805,"end":811},"obj":"NN"},{"id":"T7276","span":{"begin":817,"end":819},"obj":"CD"},{"id":"T7277","span":{"begin":811,"end":813},"obj":","},{"id":"T7278","span":{"begin":813,"end":816},"obj":"CD"},{"id":"T7279","span":{"begin":816,"end":817},"obj":"HYPH"},{"id":"T7280","span":{"begin":819,"end":821},"obj":","},{"id":"T7281","span":{"begin":821,"end":826},"obj":"NN"},{"id":"T7282","span":{"begin":827,"end":831},"obj":"NN"},{"id":"T7283","span":{"begin":832,"end":836},"obj":"IN"},{"id":"T7284","span":{"begin":837,"end":840},"obj":"CD"},{"id":"T7285","span":{"begin":841,"end":849},"obj":"NNS"},{"id":"T7286","span":{"begin":849,"end":850},"obj":"SYM"},{"id":"T7287","span":{"begin":850,"end":854},"obj":"NN"},{"id":"T7288","span":{"begin":854,"end":856},"obj":","},{"id":"T7289","span":{"begin":856,"end":859},"obj":"CD"},{"id":"T7290","span":{"begin":860,"end":862},"obj":"NN"},{"id":"T7291","span":{"begin":863,"end":870},"obj":"IN"},{"id":"T7292","span":{"begin":871,"end":879},"obj":"NNS"},{"id":"T7293","span":{"begin":879,"end":881},"obj":","},{"id":"T7294","span":{"begin":881,"end":884},"obj":"CC"},{"id":"T7295","span":{"begin":885,"end":892},"obj":"CD"},{"id":"T7296","span":{"begin":893,"end":895},"obj":"NN"},{"id":"T7297","span":{"begin":908,"end":912},"obj":"NN"},{"id":"T7298","span":{"begin":896,"end":907},"obj":"NN"},{"id":"T7299","span":{"begin":912,"end":913},"obj":"."}],"relations":[{"id":"R4453","pred":"nummod","subj":"T7121","obj":"T7122"},{"id":"R4454","pred":"compound","subj":"T7122","obj":"T7123"},{"id":"R4455","pred":"nsubjpass","subj":"T7123","obj":"T7126"},{"id":"R4456","pred":"compound","subj":"T7124","obj":"T7125"},{"id":"R4457","pred":"compound","subj":"T7125","obj":"T7123"},{"id":"R4458","pred":"punct","subj":"T7127","obj":"T7123"},{"id":"R4459","pred":"dep","subj":"T7128","obj":"T7129"},{"id":"R4460","pred":"relcl","subj":"T7129","obj":"T7123"},{"id":"R4461","pred":"advmod","subj":"T7130","obj":"T7131"},{"id":"R4462","pred":"nummod","subj":"T7131","obj":"T7134"},{"id":"R4463","pred":"quantmod","subj":"T7132","obj":"T7131"},{"id":"R4464","pred":"punct","subj":"T7133","obj":"T7131"},{"id":"R4465","pred":"npadvmod","subj":"T7134","obj":"T7135"},{"id":"R4466","pred":"acomp","subj":"T7135","obj":"T7129"},{"id":"R4467","pred":"punct","subj":"T7136","obj":"T7126"},{"id":"R4468","pred":"auxpass","subj":"T7137","obj":"T7126"},{"id":"R4469","pred":"advmod","subj":"T7138","obj":"T7126"},{"id":"R4470","pred":"prep","subj":"T7139","obj":"T7126"},{"id":"R4471","pred":"npadvmod","subj":"T7140","obj":"T7141"},{"id":"R4472","pred":"amod","subj":"T7141","obj":"T7143"},{"id":"R4473","pred":"punct","subj":"T7142","obj":"T7141"},{"id":"R4474","pred":"pobj","subj":"T7143","obj":"T7139"},{"id":"R4475","pred":"punct","subj":"T7144","obj":"T7126"},{"id":"R4476","pred":"cc","subj":"T7145","obj":"T7126"},{"id":"R4477","pred":"amod","subj":"T7146","obj":"T7147"},{"id":"R4478","pred":"nsubjpass","subj":"T7147","obj":"T7149"},{"id":"R4479","pred":"compound","subj":"T7148","obj":"T7147"},{"id":"R4480","pred":"conj","subj":"T7149","obj":"T7126"},{"id":"R4481","pred":"auxpass","subj":"T7150","obj":"T7149"},{"id":"R4482","pred":"prep","subj":"T7151","obj":"T7149"},{"id":"R4483","pred":"pcomp","subj":"T7152","obj":"T7151"},{"id":"R4484","pred":"dobj","subj":"T7153","obj":"T7152"},{"id":"R4485","pred":"prep","subj":"T7154","obj":"T7152"},{"id":"R4486","pred":"nummod","subj":"T7155","obj":"T7156"},{"id":"R4487","pred":"compound","subj":"T7156","obj":"T7157"},{"id":"R4488","pred":"pobj","subj":"T7157","obj":"T7154"},{"id":"R4489","pred":"punct","subj":"T7158","obj":"T7157"},{"id":"R4490","pred":"npadvmod","subj":"T7159","obj":"T7157"},{"id":"R4491","pred":"nummod","subj":"T7160","obj":"T7159"},{"id":"R4512","pred":"auxpass","subj":"T7183","obj":"T7182"},{"id":"R4513","pred":"prep","subj":"T7184","obj":"T7182"},{"id":"R4514","pred":"nummod","subj":"T7185","obj":"T7186"},{"id":"R4515","pred":"pobj","subj":"T7186","obj":"T7184"},{"id":"R4516","pred":"punct","subj":"T7187","obj":"T7182"},{"id":"R4517","pred":"det","subj":"T7188","obj":"T7189"},{"id":"R4518","pred":"nsubjpass","subj":"T7189","obj":"T7190"},{"id":"R4520","pred":"auxpass","subj":"T7191","obj":"T7190"},{"id":"R4523","pred":"compound","subj":"T7194","obj":"T7195"},{"id":"R4524","pred":"nsubjpass","subj":"T7195","obj":"T7196"},{"id":"R4525","pred":"conj","subj":"T7196","obj":"T7190"},{"id":"R4526","pred":"auxpass","subj":"T7197","obj":"T7196"},{"id":"R4527","pred":"advcl","subj":"T7198","obj":"T7196"},{"id":"R4528","pred":"det","subj":"T7199","obj":"T7200"},{"id":"R4529","pred":"dobj","subj":"T7200","obj":"T7198"},{"id":"R4530","pred":"compound","subj":"T7201","obj":"T7202"},{"id":"R4531","pred":"compound","subj":"T7202","obj":"T7200"},{"id":"R4532","pred":"punct","subj":"T7203","obj":"T7202"},{"id":"R4533","pred":"compound","subj":"T7204","obj":"T7200"},{"id":"R4534","pred":"compound","subj":"T7205","obj":"T7200"},{"id":"R4535","pred":"punct","subj":"T7206","obj":"T7196"},{"id":"R4536","pred":"quantmod","subj":"T7208","obj":"T7209"},{"id":"R4537","pred":"nummod","subj":"T7209","obj":"T7211"},{"id":"R4538","pred":"punct","subj":"T7210","obj":"T7209"},{"id":"R4539","pred":"nsubjpass","subj":"T7211","obj":"T7212"},{"id":"R4540","pred":"prep","subj":"T7213","obj":"T7211"},{"id":"R4541","pred":"amod","subj":"T7214","obj":"T7215"},{"id":"R4542","pred":"pobj","subj":"T7215","obj":"T7213"},{"id":"R4543","pred":"compound","subj":"T7216","obj":"T7215"},{"id":"R4544","pred":"auxpass","subj":"T7217","obj":"T7212"},{"id":"R4545","pred":"aux","subj":"T7218","obj":"T7219"},{"id":"R4546","pred":"advcl","subj":"T7219","obj":"T7212"},{"id":"R4547","pred":"compound","subj":"T7220","obj":"T7221"},{"id":"R4548","pred":"compound","subj":"T7221","obj":"T7222"},{"id":"R4549","pred":"dobj","subj":"T7222","obj":"T7219"},{"id":"R4550","pred":"advcl","subj":"T7223","obj":"T7219"},{"id":"R4551","pred":"det","subj":"T7224","obj":"T7225"},{"id":"R4552","pred":"dobj","subj":"T7225","obj":"T7223"},{"id":"R4553","pred":"amod","subj":"T7226","obj":"T7227"},{"id":"R4554","pred":"compound","subj":"T7227","obj":"T7225"},{"id":"R4555","pred":"nmod","subj":"T7228","obj":"T7227"},{"id":"R4556","pred":"punct","subj":"T7229","obj":"T7227"},{"id":"R4557","pred":"compound","subj":"T7230","obj":"T7227"},{"id":"R4558","pred":"compound","subj":"T7231","obj":"T7225"},{"id":"R4559","pred":"compound","subj":"T7232","obj":"T7225"},{"id":"R4560","pred":"compound","subj":"T7233","obj":"T7225"},{"id":"R4561","pred":"punct","subj":"T7234","obj":"T7235"},{"id":"R4562","pred":"parataxis","subj":"T7235","obj":"T7225"},{"id":"R4563","pred":"compound","subj":"T7236","obj":"T7235"},{"id":"R4564","pred":"punct","subj":"T7237","obj":"T7235"},{"id":"R4565","pred":"prep","subj":"T7238","obj":"T7223"},{"id":"R4566","pred":"prep","subj":"T7239","obj":"T7238"},{"id":"R4567","pred":"det","subj":"T7240","obj":"T7241"},{"id":"R4568","pred":"poss","subj":"T7241","obj":"T7242"},{"id":"R4569","pred":"pobj","subj":"T7242","obj":"T7239"},{"id":"R4570","pred":"case","subj":"T7243","obj":"T7241"},{"id":"R4571","pred":"punct","subj":"T7244","obj":"T7212"},{"id":"R4572","pred":"amod","subj":"T7246","obj":"T7247"},{"id":"R4573","pred":"nsubjpass","subj":"T7247","obj":"T7248"},{"id":"R4574","pred":"auxpass","subj":"T7249","obj":"T7248"},{"id":"R4575","pred":"advcl","subj":"T7250","obj":"T7248"},{"id":"R4576","pred":"det","subj":"T7251","obj":"T7252"},{"id":"R4577","pred":"dobj","subj":"T7252","obj":"T7250"},{"id":"R4578","pred":"compound","subj":"T7253","obj":"T7254"},{"id":"R4579","pred":"compound","subj":"T7254","obj":"T7252"},{"id":"R4580","pred":"compound","subj":"T7255","obj":"T7252"},{"id":"R4581","pred":"punct","subj":"T7256","obj":"T7257"},{"id":"R4582","pred":"parataxis","subj":"T7257","obj":"T7252"},{"id":"R4583","pred":"amod","subj":"T7258","obj":"T7257"},{"id":"R4584","pred":"punct","subj":"T7259","obj":"T7257"},{"id":"R4585","pred":"prep","subj":"T7260","obj":"T7250"},{"id":"R4586","pred":"det","subj":"T7261","obj":"T7262"},{"id":"R4587","pred":"pobj","subj":"T7262","obj":"T7260"},{"id":"R4588","pred":"amod","subj":"T7263","obj":"T7262"},{"id":"R4589","pred":"cc","subj":"T7264","obj":"T7262"},{"id":"R4590","pred":"conj","subj":"T7265","obj":"T7262"},{"id":"R4591","pred":"punct","subj":"T7266","obj":"T7248"},{"id":"R4592","pred":"compound","subj":"T7267","obj":"T7268"},{"id":"R4593","pred":"dep","subj":"T7268","obj":"T7269"},{"id":"R4602","pred":"punct","subj":"T7277","obj":"T7276"},{"id":"R4603","pred":"nummod","subj":"T7278","obj":"T7276"},{"id":"R4604","pred":"punct","subj":"T7279","obj":"T7276"},{"id":"R4605","pred":"punct","subj":"T7280","obj":"T7276"},{"id":"R4606","pred":"compound","subj":"T7281","obj":"T7282"},{"id":"R4607","pred":"conj","subj":"T7282","obj":"T7276"},{"id":"R4608","pred":"prep","subj":"T7283","obj":"T7282"},{"id":"R4609","pred":"nummod","subj":"T7284","obj":"T7285"},{"id":"R4610","pred":"pobj","subj":"T7285","obj":"T7283"},{"id":"R4611","pred":"punct","subj":"T7286","obj":"T7287"},{"id":"R4612","pred":"prep","subj":"T7287","obj":"T7285"},{"id":"R4613","pred":"punct","subj":"T7288","obj":"T7282"},{"id":"R4614","pred":"nummod","subj":"T7289","obj":"T7290"},{"id":"R4615","pred":"conj","subj":"T7290","obj":"T7282"},{"id":"R4616","pred":"prep","subj":"T7291","obj":"T7290"},{"id":"R4617","pred":"pobj","subj":"T7292","obj":"T7291"},{"id":"R4618","pred":"punct","subj":"T7293","obj":"T7290"},{"id":"R4619","pred":"cc","subj":"T7294","obj":"T7290"},{"id":"R4620","pred":"nummod","subj":"T7295","obj":"T7296"},{"id":"R4621","pred":"compound","subj":"T7296","obj":"T7297"},{"id":"R4622","pred":"conj","subj":"T7297","obj":"T7290"},{"id":"R4623","pred":"compound","subj":"T7298","obj":"T7297"},{"id":"R4624","pred":"punct","subj":"T7299","obj":"T7248"},{"id":"R4492","pred":"punct","subj":"T7161","obj":"T7157"},{"id":"R4493","pred":"nummod","subj":"T7162","obj":"T7163"},{"id":"R4494","pred":"compound","subj":"T7163","obj":"T7164"},{"id":"R4495","pred":"appos","subj":"T7164","obj":"T7157"},{"id":"R4496","pred":"compound","subj":"T7165","obj":"T7164"},{"id":"R4497","pred":"punct","subj":"T7166","obj":"T7164"},{"id":"R4498","pred":"nummod","subj":"T7167","obj":"T7164"},{"id":"R4499","pred":"acl","subj":"T7168","obj":"T7157"},{"id":"R4500","pred":"agent","subj":"T7169","obj":"T7168"},{"id":"R4501","pred":"pobj","subj":"T7170","obj":"T7169"},{"id":"R4502","pred":"prep","subj":"T7171","obj":"T7170"},{"id":"R4503","pred":"pobj","subj":"T7172","obj":"T7171"},{"id":"R4504","pred":"punct","subj":"T7173","obj":"T7170"},{"id":"R4505","pred":"prep","subj":"T7174","obj":"T7170"},{"id":"R4506","pred":"nummod","subj":"T7175","obj":"T7176"},{"id":"R4507","pred":"pobj","subj":"T7176","obj":"T7174"},{"id":"R4508","pred":"punct","subj":"T7177","obj":"T7149"},{"id":"R4509","pred":"det","subj":"T7179","obj":"T7180"},{"id":"R4511","pred":"amod","subj":"T7181","obj":"T7180"},{"id":"R4510","pred":"nsubjpass","subj":"T7180","obj":"T7182"},{"id":"R4519","pred":"conj","subj":"T7190","obj":"T7182"},{"id":"R4521","pred":"punct","subj":"T7192","obj":"T7190"},{"id":"R4522","pred":"cc","subj":"T7193","obj":"T7190"},{"id":"R4594","pred":"dep","subj":"T7269","obj":"T7248"},{"id":"R4595","pred":"punct","subj":"T7270","obj":"T7269"},{"id":"R4596","pred":"nummod","subj":"T7271","obj":"T7269"},{"id":"R4597","pred":"punct","subj":"T7272","obj":"T7269"},{"id":"R4598","pred":"punct","subj":"T7273","obj":"T7269"},{"id":"R4599","pred":"compound","subj":"T7274","obj":"T7275"},{"id":"R4600","pred":"dep","subj":"T7275","obj":"T7276"},{"id":"R4601","pred":"conj","subj":"T7276","obj":"T7269"}],"text":"100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes. The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay. 50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations. Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time."}

    craft-ca-core-ex-dev

    {"project":"craft-ca-core-ex-dev","denotations":[{"id":"T7104","span":{"begin":7,"end":13},"obj":"UBERON:0000479"},{"id":"T7105","span":{"begin":122,"end":129},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7106","span":{"begin":165,"end":170},"obj":"CL_GO_EXT:cell"},{"id":"T7107","span":{"begin":182,"end":186},"obj":"CHEBI:9754"},{"id":"T7108","span":{"begin":201,"end":213},"obj":"CHEBI:9750"},{"id":"T7109","span":{"begin":347,"end":354},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7110","span":{"begin":405,"end":412},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7111","span":{"begin":438,"end":445},"obj":"CHEBI_PR_EXT:protein"},{"id":"T7112","span":{"begin":475,"end":486},"obj":"PR_EXT:000006025"},{"id":"T7113","span":{"begin":506,"end":517},"obj":"CHEBI_EXT:76219"},{"id":"T7114","span":{"begin":528,"end":539},"obj":"PR_EXT:000006025"},{"id":"T7115","span":{"begin":709,"end":718},"obj":"CHEBI_EXT:polyatomic_entity_or_group"}],"text":"100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes. The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay. 50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations. Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time."}