Id |
Subject |
Object |
Predicate |
Lexical cue |
T7121 |
0-3 |
CD |
denotes |
100 |
T7122 |
4-6 |
NN |
denotes |
mm |
T7123 |
22-28 |
NNS |
denotes |
dishes |
T7124 |
7-13 |
NN |
denotes |
tissue |
T7125 |
14-21 |
NN |
denotes |
culture |
T7126 |
78-84 |
VBN |
denotes |
washed |
T7127 |
28-30 |
, |
denotes |
, |
T7128 |
30-35 |
WDT |
denotes |
which |
T7129 |
36-40 |
VBD |
denotes |
were |
T7130 |
41-54 |
RB |
denotes |
approximately |
T7131 |
58-60 |
CD |
denotes |
90 |
T7132 |
55-57 |
CD |
denotes |
80 |
T7133 |
57-58 |
SYM |
denotes |
– |
T7134 |
60-61 |
NN |
denotes |
% |
T7135 |
62-71 |
JJ |
denotes |
confluent |
T7136 |
71-73 |
, |
denotes |
, |
T7137 |
73-77 |
VBD |
denotes |
were |
T7138 |
85-92 |
RB |
denotes |
briefly |
T7139 |
93-97 |
IN |
denotes |
with |
T7140 |
98-101 |
NN |
denotes |
ice |
T7141 |
102-106 |
JJ |
denotes |
cold |
T7142 |
101-102 |
HYPH |
denotes |
- |
T7143 |
107-110 |
NN |
denotes |
PBS |
T7144 |
110-112 |
, |
denotes |
, |
T7145 |
112-115 |
CC |
denotes |
and |
T7146 |
116-121 |
JJ |
denotes |
total |
T7147 |
130-138 |
NNS |
denotes |
extracts |
T7148 |
122-129 |
NN |
denotes |
protein |
T7149 |
144-152 |
VBN |
denotes |
isolated |
T7150 |
139-143 |
VBD |
denotes |
were |
T7151 |
153-155 |
IN |
denotes |
by |
T7152 |
156-164 |
VBG |
denotes |
scraping |
T7153 |
165-170 |
NNS |
denotes |
cells |
T7154 |
171-175 |
IN |
denotes |
into |
T7155 |
176-178 |
CD |
denotes |
10 |
T7156 |
179-181 |
NN |
denotes |
mM |
T7157 |
182-186 |
NN |
denotes |
Tris |
T7158 |
186-188 |
, |
denotes |
, |
T7159 |
188-190 |
NN |
denotes |
pH |
T7160 |
191-194 |
CD |
denotes |
7.4 |
T7161 |
194-196 |
, |
denotes |
, |
T7162 |
196-199 |
CD |
denotes |
0.1 |
T7163 |
199-200 |
NN |
denotes |
% |
T7164 |
208-209 |
NN |
denotes |
X |
T7165 |
201-207 |
NN |
denotes |
Triton |
T7166 |
209-210 |
HYPH |
denotes |
- |
T7167 |
210-213 |
CD |
denotes |
100 |
T7168 |
214-222 |
VBN |
denotes |
followed |
T7169 |
223-225 |
IN |
denotes |
by |
T7170 |
226-236 |
NN |
denotes |
incubation |
T7171 |
237-239 |
IN |
denotes |
on |
T7172 |
240-243 |
NN |
denotes |
ice |
T7173 |
243-245 |
, |
denotes |
, |
T7174 |
245-248 |
IN |
denotes |
for |
T7175 |
249-251 |
CD |
denotes |
20 |
T7176 |
252-259 |
NNS |
denotes |
minutes |
T7177 |
259-260 |
. |
denotes |
. |
T7178 |
260-419 |
sentence |
denotes |
The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay. |
T7179 |
261-264 |
DT |
denotes |
The |
T7180 |
275-283 |
NN |
denotes |
material |
T7181 |
265-274 |
JJ |
denotes |
insoluble |
T7182 |
288-299 |
VBN |
denotes |
centrifuged |
T7183 |
284-287 |
VBD |
denotes |
was |
T7184 |
300-302 |
IN |
denotes |
at |
T7185 |
303-309 |
CD |
denotes |
14,000 |
T7186 |
310-311 |
NN |
denotes |
g |
T7187 |
311-313 |
, |
denotes |
, |
T7188 |
313-316 |
DT |
denotes |
the |
T7189 |
317-328 |
NN |
denotes |
supernatant |
T7190 |
333-341 |
VBN |
denotes |
isolated |
T7191 |
329-332 |
VBD |
denotes |
was |
T7192 |
341-343 |
, |
denotes |
, |
T7193 |
343-346 |
CC |
denotes |
and |
T7194 |
347-354 |
NN |
denotes |
protein |
T7195 |
355-368 |
NN |
denotes |
concentration |
T7196 |
373-383 |
VBN |
denotes |
determined |
T7197 |
369-372 |
VBD |
denotes |
was |
T7198 |
384-389 |
VBG |
denotes |
using |
T7199 |
390-393 |
DT |
denotes |
the |
T7200 |
413-418 |
NN |
denotes |
Assay |
T7201 |
394-397 |
NNP |
denotes |
Bio |
T7202 |
398-401 |
NNP |
denotes |
rad |
T7203 |
397-398 |
HYPH |
denotes |
- |
T7204 |
402-404 |
NN |
denotes |
Dc |
T7205 |
405-412 |
NN |
denotes |
Protein |
T7206 |
418-419 |
. |
denotes |
. |
T7207 |
419-639 |
sentence |
denotes |
50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations. |
T7208 |
420-422 |
CD |
denotes |
50 |
T7209 |
423-425 |
CD |
denotes |
70 |
T7210 |
422-423 |
SYM |
denotes |
– |
T7211 |
426-428 |
NN |
denotes |
μg |
T7212 |
459-463 |
VBN |
denotes |
used |
T7213 |
429-431 |
IN |
denotes |
of |
T7214 |
432-437 |
JJ |
denotes |
total |
T7215 |
446-453 |
NN |
denotes |
extract |
T7216 |
438-445 |
NN |
denotes |
protein |
T7217 |
454-458 |
VBD |
denotes |
were |
T7218 |
464-466 |
TO |
denotes |
to |
T7219 |
467-474 |
VB |
denotes |
measure |
T7220 |
475-484 |
NN |
denotes |
cathepsin |
T7221 |
485-486 |
NN |
denotes |
D |
T7222 |
487-495 |
NN |
denotes |
activity |
T7223 |
496-501 |
VBG |
denotes |
using |
T7224 |
502-505 |
DT |
denotes |
the |
T7225 |
569-572 |
NN |
denotes |
Kit |
T7226 |
506-517 |
JJ |
denotes |
Fluorogenic |
T7227 |
538-539 |
NN |
denotes |
D |
T7228 |
518-526 |
NNP |
denotes |
Innozyme |
T7229 |
526-527 |
SYM |
denotes |
™ |
T7230 |
528-537 |
NN |
denotes |
Cathepsin |
T7231 |
540-553 |
NN |
denotes |
Immunocapture |
T7232 |
554-562 |
NN |
denotes |
Activity |
T7233 |
563-568 |
NN |
denotes |
Assay |
T7234 |
573-574 |
-LRB- |
denotes |
( |
T7235 |
578-589 |
NNP |
denotes |
Biosciences |
T7236 |
574-577 |
NNP |
denotes |
EMD |
T7237 |
589-590 |
-RRB- |
denotes |
) |
T7238 |
591-600 |
VBG |
denotes |
according |
T7239 |
601-603 |
IN |
denotes |
to |
T7240 |
604-607 |
DT |
denotes |
the |
T7241 |
608-620 |
NN |
denotes |
manufacturer |
T7242 |
623-638 |
NNS |
denotes |
recommendations |
T7243 |
620-622 |
POS |
denotes |
's |
T7244 |
638-639 |
. |
denotes |
. |
T7245 |
639-913 |
sentence |
denotes |
Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time. |
T7246 |
640-648 |
JJ |
denotes |
Relative |
T7247 |
649-661 |
NN |
denotes |
fluorescence |
T7248 |
666-674 |
VBN |
denotes |
measured |
T7249 |
662-665 |
VBD |
denotes |
was |
T7250 |
675-680 |
VBG |
denotes |
using |
T7251 |
681-683 |
DT |
denotes |
an |
T7252 |
701-707 |
NN |
denotes |
reader |
T7253 |
684-691 |
NN |
denotes |
Analyst |
T7254 |
692-694 |
NN |
denotes |
AD |
T7255 |
695-700 |
NN |
denotes |
plate |
T7256 |
708-709 |
-LRB- |
denotes |
( |
T7257 |
719-726 |
NNPS |
denotes |
Devices |
T7258 |
709-718 |
JJ |
denotes |
Molecular |
T7259 |
726-727 |
-RRB- |
denotes |
) |
T7260 |
728-732 |
IN |
denotes |
with |
T7261 |
733-736 |
DT |
denotes |
the |
T7262 |
747-754 |
NNS |
denotes |
filters |
T7263 |
737-746 |
JJ |
denotes |
following |
T7264 |
755-758 |
CC |
denotes |
and |
T7265 |
759-767 |
NNS |
denotes |
settings |
T7266 |
767-769 |
: |
denotes |
: |
T7267 |
769-779 |
NN |
denotes |
excitation |
T7268 |
780-786 |
NN |
denotes |
filter |
T7269 |
792-794 |
CD |
denotes |
35 |
T7270 |
786-788 |
, |
denotes |
, |
T7271 |
788-791 |
CD |
denotes |
360 |
T7272 |
791-792 |
HYPH |
denotes |
- |
T7273 |
794-795 |
: |
denotes |
; |
T7274 |
796-804 |
NN |
denotes |
emission |
T7275 |
805-811 |
NN |
denotes |
filter |
T7276 |
817-819 |
CD |
denotes |
20 |
T7277 |
811-813 |
, |
denotes |
, |
T7278 |
813-816 |
CD |
denotes |
400 |
T7279 |
816-817 |
HYPH |
denotes |
- |
T7280 |
819-821 |
, |
denotes |
, |
T7281 |
821-826 |
NN |
denotes |
Flash |
T7282 |
827-831 |
NN |
denotes |
lamp |
T7283 |
832-836 |
IN |
denotes |
with |
T7284 |
837-840 |
CD |
denotes |
100 |
T7285 |
841-849 |
NNS |
denotes |
readings |
T7286 |
849-850 |
SYM |
denotes |
/ |
T7287 |
850-854 |
NN |
denotes |
well |
T7288 |
854-856 |
, |
denotes |
, |
T7289 |
856-859 |
CD |
denotes |
100 |
T7290 |
860-862 |
NN |
denotes |
ms |
T7291 |
863-870 |
IN |
denotes |
between |
T7292 |
871-879 |
NNS |
denotes |
readings |
T7293 |
879-881 |
, |
denotes |
, |
T7294 |
881-884 |
CC |
denotes |
and |
T7295 |
885-892 |
CD |
denotes |
100,000 |
T7296 |
893-895 |
NN |
denotes |
μs |
T7297 |
908-912 |
NN |
denotes |
time |
T7298 |
896-907 |
NN |
denotes |
integration |
T7299 |
912-913 |
. |
denotes |
. |
R4453 |
T7121 |
T7122 |
nummod |
100,mm |
R4454 |
T7122 |
T7123 |
compound |
mm,dishes |
R4455 |
T7123 |
T7126 |
nsubjpass |
dishes,washed |
R4456 |
T7124 |
T7125 |
compound |
tissue,culture |
R4457 |
T7125 |
T7123 |
compound |
culture,dishes |
R4458 |
T7127 |
T7123 |
punct |
", ",dishes |
R4459 |
T7128 |
T7129 |
dep |
which,were |
R4460 |
T7129 |
T7123 |
relcl |
were,dishes |
R4461 |
T7130 |
T7131 |
advmod |
approximately,90 |
R4462 |
T7131 |
T7134 |
nummod |
90,% |
R4463 |
T7132 |
T7131 |
quantmod |
80,90 |
R4464 |
T7133 |
T7131 |
punct |
–,90 |
R4465 |
T7134 |
T7135 |
npadvmod |
%,confluent |
R4466 |
T7135 |
T7129 |
acomp |
confluent,were |
R4467 |
T7136 |
T7126 |
punct |
", ",washed |
R4468 |
T7137 |
T7126 |
auxpass |
were,washed |
R4469 |
T7138 |
T7126 |
advmod |
briefly,washed |
R4470 |
T7139 |
T7126 |
prep |
with,washed |
R4471 |
T7140 |
T7141 |
npadvmod |
ice,cold |
R4472 |
T7141 |
T7143 |
amod |
cold,PBS |
R4473 |
T7142 |
T7141 |
punct |
-,cold |
R4474 |
T7143 |
T7139 |
pobj |
PBS,with |
R4475 |
T7144 |
T7126 |
punct |
", ",washed |
R4476 |
T7145 |
T7126 |
cc |
and,washed |
R4477 |
T7146 |
T7147 |
amod |
total,extracts |
R4478 |
T7147 |
T7149 |
nsubjpass |
extracts,isolated |
R4479 |
T7148 |
T7147 |
compound |
protein,extracts |
R4480 |
T7149 |
T7126 |
conj |
isolated,washed |
R4481 |
T7150 |
T7149 |
auxpass |
were,isolated |
R4482 |
T7151 |
T7149 |
prep |
by,isolated |
R4483 |
T7152 |
T7151 |
pcomp |
scraping,by |
R4484 |
T7153 |
T7152 |
dobj |
cells,scraping |
R4485 |
T7154 |
T7152 |
prep |
into,scraping |
R4486 |
T7155 |
T7156 |
nummod |
10,mM |
R4487 |
T7156 |
T7157 |
compound |
mM,Tris |
R4488 |
T7157 |
T7154 |
pobj |
Tris,into |
R4489 |
T7158 |
T7157 |
punct |
", ",Tris |
R4490 |
T7159 |
T7157 |
npadvmod |
pH,Tris |
R4491 |
T7160 |
T7159 |
nummod |
7.4,pH |
R4512 |
T7183 |
T7182 |
auxpass |
was,centrifuged |
R4513 |
T7184 |
T7182 |
prep |
at,centrifuged |
R4514 |
T7185 |
T7186 |
nummod |
"14,000",g |
R4515 |
T7186 |
T7184 |
pobj |
g,at |
R4516 |
T7187 |
T7182 |
punct |
", ",centrifuged |
R4517 |
T7188 |
T7189 |
det |
the,supernatant |
R4518 |
T7189 |
T7190 |
nsubjpass |
supernatant,isolated |
R4520 |
T7191 |
T7190 |
auxpass |
was,isolated |
R4523 |
T7194 |
T7195 |
compound |
protein,concentration |
R4524 |
T7195 |
T7196 |
nsubjpass |
concentration,determined |
R4525 |
T7196 |
T7190 |
conj |
determined,isolated |
R4526 |
T7197 |
T7196 |
auxpass |
was,determined |
R4527 |
T7198 |
T7196 |
advcl |
using,determined |
R4528 |
T7199 |
T7200 |
det |
the,Assay |
R4529 |
T7200 |
T7198 |
dobj |
Assay,using |
R4530 |
T7201 |
T7202 |
compound |
Bio,rad |
R4531 |
T7202 |
T7200 |
compound |
rad,Assay |
R4532 |
T7203 |
T7202 |
punct |
-,rad |
R4533 |
T7204 |
T7200 |
compound |
Dc,Assay |
R4534 |
T7205 |
T7200 |
compound |
Protein,Assay |
R4535 |
T7206 |
T7196 |
punct |
.,determined |
R4536 |
T7208 |
T7209 |
quantmod |
50,70 |
R4537 |
T7209 |
T7211 |
nummod |
70,μg |
R4538 |
T7210 |
T7209 |
punct |
–,70 |
R4539 |
T7211 |
T7212 |
nsubjpass |
μg,used |
R4540 |
T7213 |
T7211 |
prep |
of,μg |
R4541 |
T7214 |
T7215 |
amod |
total,extract |
R4542 |
T7215 |
T7213 |
pobj |
extract,of |
R4543 |
T7216 |
T7215 |
compound |
protein,extract |
R4544 |
T7217 |
T7212 |
auxpass |
were,used |
R4545 |
T7218 |
T7219 |
aux |
to,measure |
R4546 |
T7219 |
T7212 |
advcl |
measure,used |
R4547 |
T7220 |
T7221 |
compound |
cathepsin,D |
R4548 |
T7221 |
T7222 |
compound |
D,activity |
R4549 |
T7222 |
T7219 |
dobj |
activity,measure |
R4550 |
T7223 |
T7219 |
advcl |
using,measure |
R4551 |
T7224 |
T7225 |
det |
the,Kit |
R4552 |
T7225 |
T7223 |
dobj |
Kit,using |
R4553 |
T7226 |
T7227 |
amod |
Fluorogenic,D |
R4554 |
T7227 |
T7225 |
compound |
D,Kit |
R4555 |
T7228 |
T7227 |
nmod |
Innozyme,D |
R4556 |
T7229 |
T7227 |
punct |
™,D |
R4557 |
T7230 |
T7227 |
compound |
Cathepsin,D |
R4558 |
T7231 |
T7225 |
compound |
Immunocapture,Kit |
R4559 |
T7232 |
T7225 |
compound |
Activity,Kit |
R4560 |
T7233 |
T7225 |
compound |
Assay,Kit |
R4561 |
T7234 |
T7235 |
punct |
(,Biosciences |
R4562 |
T7235 |
T7225 |
parataxis |
Biosciences,Kit |
R4563 |
T7236 |
T7235 |
compound |
EMD,Biosciences |
R4564 |
T7237 |
T7235 |
punct |
),Biosciences |
R4565 |
T7238 |
T7223 |
prep |
according,using |
R4566 |
T7239 |
T7238 |
prep |
to,according |
R4567 |
T7240 |
T7241 |
det |
the,manufacturer |
R4568 |
T7241 |
T7242 |
poss |
manufacturer,recommendations |
R4569 |
T7242 |
T7239 |
pobj |
recommendations,to |
R4570 |
T7243 |
T7241 |
case |
's,manufacturer |
R4571 |
T7244 |
T7212 |
punct |
.,used |
R4572 |
T7246 |
T7247 |
amod |
Relative,fluorescence |
R4573 |
T7247 |
T7248 |
nsubjpass |
fluorescence,measured |
R4574 |
T7249 |
T7248 |
auxpass |
was,measured |
R4575 |
T7250 |
T7248 |
advcl |
using,measured |
R4576 |
T7251 |
T7252 |
det |
an,reader |
R4577 |
T7252 |
T7250 |
dobj |
reader,using |
R4578 |
T7253 |
T7254 |
compound |
Analyst,AD |
R4579 |
T7254 |
T7252 |
compound |
AD,reader |
R4580 |
T7255 |
T7252 |
compound |
plate,reader |
R4581 |
T7256 |
T7257 |
punct |
(,Devices |
R4582 |
T7257 |
T7252 |
parataxis |
Devices,reader |
R4583 |
T7258 |
T7257 |
amod |
Molecular,Devices |
R4584 |
T7259 |
T7257 |
punct |
),Devices |
R4585 |
T7260 |
T7250 |
prep |
with,using |
R4586 |
T7261 |
T7262 |
det |
the,filters |
R4587 |
T7262 |
T7260 |
pobj |
filters,with |
R4588 |
T7263 |
T7262 |
amod |
following,filters |
R4589 |
T7264 |
T7262 |
cc |
and,filters |
R4590 |
T7265 |
T7262 |
conj |
settings,filters |
R4591 |
T7266 |
T7248 |
punct |
: ,measured |
R4592 |
T7267 |
T7268 |
compound |
excitation,filter |
R4593 |
T7268 |
T7269 |
dep |
filter,35 |
R4602 |
T7277 |
T7276 |
punct |
", ",20 |
R4603 |
T7278 |
T7276 |
nummod |
400,20 |
R4604 |
T7279 |
T7276 |
punct |
-,20 |
R4605 |
T7280 |
T7276 |
punct |
", ",20 |
R4606 |
T7281 |
T7282 |
compound |
Flash,lamp |
R4607 |
T7282 |
T7276 |
conj |
lamp,20 |
R4608 |
T7283 |
T7282 |
prep |
with,lamp |
R4609 |
T7284 |
T7285 |
nummod |
100,readings |
R4610 |
T7285 |
T7283 |
pobj |
readings,with |
R4611 |
T7286 |
T7287 |
punct |
/,well |
R4612 |
T7287 |
T7285 |
prep |
well,readings |
R4613 |
T7288 |
T7282 |
punct |
", ",lamp |
R4614 |
T7289 |
T7290 |
nummod |
100,ms |
R4615 |
T7290 |
T7282 |
conj |
ms,lamp |
R4616 |
T7291 |
T7290 |
prep |
between,ms |
R4617 |
T7292 |
T7291 |
pobj |
readings,between |
R4618 |
T7293 |
T7290 |
punct |
", ",ms |
R4619 |
T7294 |
T7290 |
cc |
and,ms |
R4620 |
T7295 |
T7296 |
nummod |
"100,000",μs |
R4621 |
T7296 |
T7297 |
compound |
μs,time |
R4622 |
T7297 |
T7290 |
conj |
time,ms |
R4623 |
T7298 |
T7297 |
compound |
integration,time |
R4624 |
T7299 |
T7248 |
punct |
.,measured |
R4492 |
T7161 |
T7157 |
punct |
", ",Tris |
R4493 |
T7162 |
T7163 |
nummod |
0.1,% |
R4494 |
T7163 |
T7164 |
compound |
%,X |
R4495 |
T7164 |
T7157 |
appos |
X,Tris |
R4496 |
T7165 |
T7164 |
compound |
Triton,X |
R4497 |
T7166 |
T7164 |
punct |
-,X |
R4498 |
T7167 |
T7164 |
nummod |
100,X |
R4499 |
T7168 |
T7157 |
acl |
followed,Tris |
R4500 |
T7169 |
T7168 |
agent |
by,followed |
R4501 |
T7170 |
T7169 |
pobj |
incubation,by |
R4502 |
T7171 |
T7170 |
prep |
on,incubation |
R4503 |
T7172 |
T7171 |
pobj |
ice,on |
R4504 |
T7173 |
T7170 |
punct |
", ",incubation |
R4505 |
T7174 |
T7170 |
prep |
for,incubation |
R4506 |
T7175 |
T7176 |
nummod |
20,minutes |
R4507 |
T7176 |
T7174 |
pobj |
minutes,for |
R4508 |
T7177 |
T7149 |
punct |
.,isolated |
R4509 |
T7179 |
T7180 |
det |
The,material |
R4511 |
T7181 |
T7180 |
amod |
insoluble,material |
R4510 |
T7180 |
T7182 |
nsubjpass |
material,centrifuged |
R4519 |
T7190 |
T7182 |
conj |
isolated,centrifuged |
R4521 |
T7192 |
T7190 |
punct |
", ",isolated |
R4522 |
T7193 |
T7190 |
cc |
and,isolated |
R4594 |
T7269 |
T7248 |
dep |
35,measured |
R4595 |
T7270 |
T7269 |
punct |
", ",35 |
R4596 |
T7271 |
T7269 |
nummod |
360,35 |
R4597 |
T7272 |
T7269 |
punct |
-,35 |
R4598 |
T7273 |
T7269 |
punct |
;,35 |
R4599 |
T7274 |
T7275 |
compound |
emission,filter |
R4600 |
T7275 |
T7276 |
dep |
filter,20 |
R4601 |
T7276 |
T7269 |
conj |
20,35 |