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PMC:539297 / 32772-33685 JSONTXT

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craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7097 7-13 UBERON:0000479 denotes tissue
T7098 182-186 CHEBI:9754 denotes Tris
T7099 201-213 CHEBI:9750 denotes Triton X-100
T7100 475-486 PR:000006025 denotes cathepsin D
T7101 506-517 CHEBI:76219 denotes Fluorogenic
T7102 528-539 PR:000006025 denotes Cathepsin D

craft-sa-dev

Id Subject Object Predicate Lexical cue
T7121 0-3 CD denotes 100
T7122 4-6 NN denotes mm
T7123 22-28 NNS denotes dishes
T7124 7-13 NN denotes tissue
T7125 14-21 NN denotes culture
T7126 78-84 VBN denotes washed
T7127 28-30 , denotes ,
T7128 30-35 WDT denotes which
T7129 36-40 VBD denotes were
T7130 41-54 RB denotes approximately
T7131 58-60 CD denotes 90
T7132 55-57 CD denotes 80
T7133 57-58 SYM denotes –
T7134 60-61 NN denotes %
T7135 62-71 JJ denotes confluent
T7136 71-73 , denotes ,
T7137 73-77 VBD denotes were
T7138 85-92 RB denotes briefly
T7139 93-97 IN denotes with
T7140 98-101 NN denotes ice
T7141 102-106 JJ denotes cold
T7142 101-102 HYPH denotes -
T7143 107-110 NN denotes PBS
T7144 110-112 , denotes ,
T7145 112-115 CC denotes and
T7146 116-121 JJ denotes total
T7147 130-138 NNS denotes extracts
T7148 122-129 NN denotes protein
T7149 144-152 VBN denotes isolated
T7150 139-143 VBD denotes were
T7151 153-155 IN denotes by
T7152 156-164 VBG denotes scraping
T7153 165-170 NNS denotes cells
T7154 171-175 IN denotes into
T7155 176-178 CD denotes 10
T7156 179-181 NN denotes mM
T7157 182-186 NN denotes Tris
T7158 186-188 , denotes ,
T7159 188-190 NN denotes pH
T7160 191-194 CD denotes 7.4
T7161 194-196 , denotes ,
T7162 196-199 CD denotes 0.1
T7163 199-200 NN denotes %
T7164 208-209 NN denotes X
T7165 201-207 NN denotes Triton
T7166 209-210 HYPH denotes -
T7167 210-213 CD denotes 100
T7168 214-222 VBN denotes followed
T7169 223-225 IN denotes by
T7170 226-236 NN denotes incubation
T7171 237-239 IN denotes on
T7172 240-243 NN denotes ice
T7173 243-245 , denotes ,
T7174 245-248 IN denotes for
T7175 249-251 CD denotes 20
T7176 252-259 NNS denotes minutes
T7177 259-260 . denotes .
T7178 260-419 sentence denotes The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay.
T7179 261-264 DT denotes The
T7180 275-283 NN denotes material
T7181 265-274 JJ denotes insoluble
T7182 288-299 VBN denotes centrifuged
T7183 284-287 VBD denotes was
T7184 300-302 IN denotes at
T7185 303-309 CD denotes 14,000
T7186 310-311 NN denotes g
T7187 311-313 , denotes ,
T7188 313-316 DT denotes the
T7189 317-328 NN denotes supernatant
T7190 333-341 VBN denotes isolated
T7191 329-332 VBD denotes was
T7192 341-343 , denotes ,
T7193 343-346 CC denotes and
T7194 347-354 NN denotes protein
T7195 355-368 NN denotes concentration
T7196 373-383 VBN denotes determined
T7197 369-372 VBD denotes was
T7198 384-389 VBG denotes using
T7199 390-393 DT denotes the
T7200 413-418 NN denotes Assay
T7201 394-397 NNP denotes Bio
T7202 398-401 NNP denotes rad
T7203 397-398 HYPH denotes -
T7204 402-404 NN denotes Dc
T7205 405-412 NN denotes Protein
T7206 418-419 . denotes .
T7207 419-639 sentence denotes 50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations.
T7208 420-422 CD denotes 50
T7209 423-425 CD denotes 70
T7210 422-423 SYM denotes –
T7211 426-428 NN denotes μg
T7212 459-463 VBN denotes used
T7213 429-431 IN denotes of
T7214 432-437 JJ denotes total
T7215 446-453 NN denotes extract
T7216 438-445 NN denotes protein
T7217 454-458 VBD denotes were
T7218 464-466 TO denotes to
T7219 467-474 VB denotes measure
T7220 475-484 NN denotes cathepsin
T7221 485-486 NN denotes D
T7222 487-495 NN denotes activity
T7223 496-501 VBG denotes using
T7224 502-505 DT denotes the
T7225 569-572 NN denotes Kit
T7226 506-517 JJ denotes Fluorogenic
T7227 538-539 NN denotes D
T7228 518-526 NNP denotes Innozyme
T7229 526-527 SYM denotes ™
T7230 528-537 NN denotes Cathepsin
T7231 540-553 NN denotes Immunocapture
T7232 554-562 NN denotes Activity
T7233 563-568 NN denotes Assay
T7234 573-574 -LRB- denotes (
T7235 578-589 NNP denotes Biosciences
T7236 574-577 NNP denotes EMD
T7237 589-590 -RRB- denotes )
T7238 591-600 VBG denotes according
T7239 601-603 IN denotes to
T7240 604-607 DT denotes the
T7241 608-620 NN denotes manufacturer
T7242 623-638 NNS denotes recommendations
T7243 620-622 POS denotes 's
T7244 638-639 . denotes .
T7245 639-913 sentence denotes Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time.
T7246 640-648 JJ denotes Relative
T7247 649-661 NN denotes fluorescence
T7248 666-674 VBN denotes measured
T7249 662-665 VBD denotes was
T7250 675-680 VBG denotes using
T7251 681-683 DT denotes an
T7252 701-707 NN denotes reader
T7253 684-691 NN denotes Analyst
T7254 692-694 NN denotes AD
T7255 695-700 NN denotes plate
T7256 708-709 -LRB- denotes (
T7257 719-726 NNPS denotes Devices
T7258 709-718 JJ denotes Molecular
T7259 726-727 -RRB- denotes )
T7260 728-732 IN denotes with
T7261 733-736 DT denotes the
T7262 747-754 NNS denotes filters
T7263 737-746 JJ denotes following
T7264 755-758 CC denotes and
T7265 759-767 NNS denotes settings
T7266 767-769 : denotes :
T7267 769-779 NN denotes excitation
T7268 780-786 NN denotes filter
T7269 792-794 CD denotes 35
T7270 786-788 , denotes ,
T7271 788-791 CD denotes 360
T7272 791-792 HYPH denotes -
T7273 794-795 : denotes ;
T7274 796-804 NN denotes emission
T7275 805-811 NN denotes filter
T7276 817-819 CD denotes 20
T7277 811-813 , denotes ,
T7278 813-816 CD denotes 400
T7279 816-817 HYPH denotes -
T7280 819-821 , denotes ,
T7281 821-826 NN denotes Flash
T7282 827-831 NN denotes lamp
T7283 832-836 IN denotes with
T7284 837-840 CD denotes 100
T7285 841-849 NNS denotes readings
T7286 849-850 SYM denotes /
T7287 850-854 NN denotes well
T7288 854-856 , denotes ,
T7289 856-859 CD denotes 100
T7290 860-862 NN denotes ms
T7291 863-870 IN denotes between
T7292 871-879 NNS denotes readings
T7293 879-881 , denotes ,
T7294 881-884 CC denotes and
T7295 885-892 CD denotes 100,000
T7296 893-895 NN denotes μs
T7297 908-912 NN denotes time
T7298 896-907 NN denotes integration
T7299 912-913 . denotes .
R4453 T7121 T7122 nummod 100,mm
R4454 T7122 T7123 compound mm,dishes
R4455 T7123 T7126 nsubjpass dishes,washed
R4456 T7124 T7125 compound tissue,culture
R4457 T7125 T7123 compound culture,dishes
R4458 T7127 T7123 punct ", ",dishes
R4459 T7128 T7129 dep which,were
R4460 T7129 T7123 relcl were,dishes
R4461 T7130 T7131 advmod approximately,90
R4462 T7131 T7134 nummod 90,%
R4463 T7132 T7131 quantmod 80,90
R4464 T7133 T7131 punct –,90
R4465 T7134 T7135 npadvmod %,confluent
R4466 T7135 T7129 acomp confluent,were
R4467 T7136 T7126 punct ", ",washed
R4468 T7137 T7126 auxpass were,washed
R4469 T7138 T7126 advmod briefly,washed
R4470 T7139 T7126 prep with,washed
R4471 T7140 T7141 npadvmod ice,cold
R4472 T7141 T7143 amod cold,PBS
R4473 T7142 T7141 punct -,cold
R4474 T7143 T7139 pobj PBS,with
R4475 T7144 T7126 punct ", ",washed
R4476 T7145 T7126 cc and,washed
R4477 T7146 T7147 amod total,extracts
R4478 T7147 T7149 nsubjpass extracts,isolated
R4479 T7148 T7147 compound protein,extracts
R4480 T7149 T7126 conj isolated,washed
R4481 T7150 T7149 auxpass were,isolated
R4482 T7151 T7149 prep by,isolated
R4483 T7152 T7151 pcomp scraping,by
R4484 T7153 T7152 dobj cells,scraping
R4485 T7154 T7152 prep into,scraping
R4486 T7155 T7156 nummod 10,mM
R4487 T7156 T7157 compound mM,Tris
R4488 T7157 T7154 pobj Tris,into
R4489 T7158 T7157 punct ", ",Tris
R4490 T7159 T7157 npadvmod pH,Tris
R4491 T7160 T7159 nummod 7.4,pH
R4512 T7183 T7182 auxpass was,centrifuged
R4513 T7184 T7182 prep at,centrifuged
R4514 T7185 T7186 nummod "14,000",g
R4515 T7186 T7184 pobj g,at
R4516 T7187 T7182 punct ", ",centrifuged
R4517 T7188 T7189 det the,supernatant
R4518 T7189 T7190 nsubjpass supernatant,isolated
R4520 T7191 T7190 auxpass was,isolated
R4523 T7194 T7195 compound protein,concentration
R4524 T7195 T7196 nsubjpass concentration,determined
R4525 T7196 T7190 conj determined,isolated
R4526 T7197 T7196 auxpass was,determined
R4527 T7198 T7196 advcl using,determined
R4528 T7199 T7200 det the,Assay
R4529 T7200 T7198 dobj Assay,using
R4530 T7201 T7202 compound Bio,rad
R4531 T7202 T7200 compound rad,Assay
R4532 T7203 T7202 punct -,rad
R4533 T7204 T7200 compound Dc,Assay
R4534 T7205 T7200 compound Protein,Assay
R4535 T7206 T7196 punct .,determined
R4536 T7208 T7209 quantmod 50,70
R4537 T7209 T7211 nummod 70,μg
R4538 T7210 T7209 punct –,70
R4539 T7211 T7212 nsubjpass μg,used
R4540 T7213 T7211 prep of,μg
R4541 T7214 T7215 amod total,extract
R4542 T7215 T7213 pobj extract,of
R4543 T7216 T7215 compound protein,extract
R4544 T7217 T7212 auxpass were,used
R4545 T7218 T7219 aux to,measure
R4546 T7219 T7212 advcl measure,used
R4547 T7220 T7221 compound cathepsin,D
R4548 T7221 T7222 compound D,activity
R4549 T7222 T7219 dobj activity,measure
R4550 T7223 T7219 advcl using,measure
R4551 T7224 T7225 det the,Kit
R4552 T7225 T7223 dobj Kit,using
R4553 T7226 T7227 amod Fluorogenic,D
R4554 T7227 T7225 compound D,Kit
R4555 T7228 T7227 nmod Innozyme,D
R4556 T7229 T7227 punct ™,D
R4557 T7230 T7227 compound Cathepsin,D
R4558 T7231 T7225 compound Immunocapture,Kit
R4559 T7232 T7225 compound Activity,Kit
R4560 T7233 T7225 compound Assay,Kit
R4561 T7234 T7235 punct (,Biosciences
R4562 T7235 T7225 parataxis Biosciences,Kit
R4563 T7236 T7235 compound EMD,Biosciences
R4564 T7237 T7235 punct ),Biosciences
R4565 T7238 T7223 prep according,using
R4566 T7239 T7238 prep to,according
R4567 T7240 T7241 det the,manufacturer
R4568 T7241 T7242 poss manufacturer,recommendations
R4569 T7242 T7239 pobj recommendations,to
R4570 T7243 T7241 case 's,manufacturer
R4571 T7244 T7212 punct .,used
R4572 T7246 T7247 amod Relative,fluorescence
R4573 T7247 T7248 nsubjpass fluorescence,measured
R4574 T7249 T7248 auxpass was,measured
R4575 T7250 T7248 advcl using,measured
R4576 T7251 T7252 det an,reader
R4577 T7252 T7250 dobj reader,using
R4578 T7253 T7254 compound Analyst,AD
R4579 T7254 T7252 compound AD,reader
R4580 T7255 T7252 compound plate,reader
R4581 T7256 T7257 punct (,Devices
R4582 T7257 T7252 parataxis Devices,reader
R4583 T7258 T7257 amod Molecular,Devices
R4584 T7259 T7257 punct ),Devices
R4585 T7260 T7250 prep with,using
R4586 T7261 T7262 det the,filters
R4587 T7262 T7260 pobj filters,with
R4588 T7263 T7262 amod following,filters
R4589 T7264 T7262 cc and,filters
R4590 T7265 T7262 conj settings,filters
R4591 T7266 T7248 punct : ,measured
R4592 T7267 T7268 compound excitation,filter
R4593 T7268 T7269 dep filter,35
R4602 T7277 T7276 punct ", ",20
R4603 T7278 T7276 nummod 400,20
R4604 T7279 T7276 punct -,20
R4605 T7280 T7276 punct ", ",20
R4606 T7281 T7282 compound Flash,lamp
R4607 T7282 T7276 conj lamp,20
R4608 T7283 T7282 prep with,lamp
R4609 T7284 T7285 nummod 100,readings
R4610 T7285 T7283 pobj readings,with
R4611 T7286 T7287 punct /,well
R4612 T7287 T7285 prep well,readings
R4613 T7288 T7282 punct ", ",lamp
R4614 T7289 T7290 nummod 100,ms
R4615 T7290 T7282 conj ms,lamp
R4616 T7291 T7290 prep between,ms
R4617 T7292 T7291 pobj readings,between
R4618 T7293 T7290 punct ", ",ms
R4619 T7294 T7290 cc and,ms
R4620 T7295 T7296 nummod "100,000",μs
R4621 T7296 T7297 compound μs,time
R4622 T7297 T7290 conj time,ms
R4623 T7298 T7297 compound integration,time
R4624 T7299 T7248 punct .,measured
R4492 T7161 T7157 punct ", ",Tris
R4493 T7162 T7163 nummod 0.1,%
R4494 T7163 T7164 compound %,X
R4495 T7164 T7157 appos X,Tris
R4496 T7165 T7164 compound Triton,X
R4497 T7166 T7164 punct -,X
R4498 T7167 T7164 nummod 100,X
R4499 T7168 T7157 acl followed,Tris
R4500 T7169 T7168 agent by,followed
R4501 T7170 T7169 pobj incubation,by
R4502 T7171 T7170 prep on,incubation
R4503 T7172 T7171 pobj ice,on
R4504 T7173 T7170 punct ", ",incubation
R4505 T7174 T7170 prep for,incubation
R4506 T7175 T7176 nummod 20,minutes
R4507 T7176 T7174 pobj minutes,for
R4508 T7177 T7149 punct .,isolated
R4509 T7179 T7180 det The,material
R4511 T7181 T7180 amod insoluble,material
R4510 T7180 T7182 nsubjpass material,centrifuged
R4519 T7190 T7182 conj isolated,centrifuged
R4521 T7192 T7190 punct ", ",isolated
R4522 T7193 T7190 cc and,isolated
R4594 T7269 T7248 dep 35,measured
R4595 T7270 T7269 punct ", ",35
R4596 T7271 T7269 nummod 360,35
R4597 T7272 T7269 punct -,35
R4598 T7273 T7269 punct ;,35
R4599 T7274 T7275 compound emission,filter
R4600 T7275 T7276 dep filter,20
R4601 T7276 T7269 conj 20,35

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T7104 7-13 UBERON:0000479 denotes tissue
T7105 122-129 CHEBI_PR_EXT:protein denotes protein
T7106 165-170 CL_GO_EXT:cell denotes cells
T7107 182-186 CHEBI:9754 denotes Tris
T7108 201-213 CHEBI:9750 denotes Triton X-100
T7109 347-354 CHEBI_PR_EXT:protein denotes protein
T7110 405-412 CHEBI_PR_EXT:protein denotes Protein
T7111 438-445 CHEBI_PR_EXT:protein denotes protein
T7112 475-486 PR_EXT:000006025 denotes cathepsin D
T7113 506-517 CHEBI_EXT:76219 denotes Fluorogenic
T7114 528-539 PR_EXT:000006025 denotes Cathepsin D
T7115 709-718 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular