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in wild-type, heterozygous and homozygous CbCln3Δex7/8 cells Dextran-FITC uptake in wild-type, heterozygous and homozygous CbCln3Δex7/8 precursor cells is shown. In wild-type (CbCln3+/+, left panel) and heterozygous (CbCln3+/Δex7/8, middle panel) cells, dextran-FITC label was observed in a perinuclear-clustered vesicular pattern with scattered labeled vesicles also present in the periphery. In contrast, dextran-FITC label of homozygous mutant (CbCln3Δex7/8/Δex7/8, right panel) cells was reduced overall and exhibited smaller stained vesicles with less perinuclear clustering. Confocal images were captured with identical exposure settings. 40 × magnification."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T9780","span":{"begin":0,"end":11},"obj":"GO:0006897"},{"id":"T9781","span":{"begin":15,"end":24},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T9782","span":{"begin":54,"end":56},"obj":"UBERON:0002037"},{"id":"T9783","span":{"begin":56,"end":60},"obj":"PR_EXT:000005591"},{"id":"T9784","span":{"begin":67,"end":72},"obj":"CL_GO_EXT:cell"},{"id":"T9785","span":{"begin":73,"end":80},"obj":"CHEBI:52071"},{"id":"T9786","span":{"begin":81,"end":85},"obj":"CHEBI:37926"},{"id":"T9787","span":{"begin":96,"end":105},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T9788","span":{"begin":135,"end":137},"obj":"UBERON:0002037"},{"id":"T9789","span":{"begin":137,"end":141},"obj":"PR_EXT:000005591"},{"id":"T9790","span":{"begin":158,"end":163},"obj":"CL_GO_EXT:cell"},{"id":"T9791","span":{"begin":177,"end":186},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T9792","span":{"begin":188,"end":190},"obj":"UBERON:0002037"},{"id":"T9793","span":{"begin":190,"end":194},"obj":"PR_EXT:000005591"},{"id":"T9794","span":{"begin":194,"end":195},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T9795","span":{"begin":196,"end":197},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T9796","span":{"begin":229,"end":231},"obj":"UBERON:0002037"},{"id":"T9797","span":{"begin":231,"end":235},"obj":"PR_EXT:000005591"},{"id":"T9798","span":{"begin":235,"end":236},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T9799","span":{"begin":259,"end":264},"obj":"CL_GO_EXT:cell"},{"id":"T9800","span":{"begin":266,"end":273},"obj":"CHEBI:52071"},{"id":"T9801","span":{"begin":274,"end":278},"obj":"CHEBI:37926"},{"id":"T9802","span":{"begin":279,"end":284},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T9803","span":{"begin":307,"end":314},"obj":"GO:0005634"},{"id":"T9804","span":{"begin":325,"end":334},"obj":"GO:0031982"},{"id":"T9805","span":{"begin":358,"end":365},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T9806","span":{"begin":366,"end":374},"obj":"GO:0031982"},{"id":"T9807","span":{"begin":419,"end":426},"obj":"CHEBI:52071"},{"id":"T9808","span":{"begin":427,"end":431},"obj":"CHEBI:37926"},{"id":"T9809","span":{"begin":432,"end":437},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T9810","span":{"begin":452,"end":458},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T9811","span":{"begin":460,"end":462},"obj":"UBERON:0002037"},{"id":"T9812","span":{"begin":462,"end":466},"obj":"PR_EXT:000005591"},{"id":"T9813","span":{"begin":494,"end":499},"obj":"CL_GO_EXT:cell"},{"id":"T9814","span":{"begin":550,"end":558},"obj":"GO:0031982"},{"id":"T9815","span":{"begin":573,"end":580},"obj":"GO:0005634"}],"text":"Endocytosis in wild-type, heterozygous and homozygous CbCln3Δex7/8 cells Dextran-FITC uptake in wild-type, heterozygous and homozygous CbCln3Δex7/8 precursor cells is shown. In wild-type (CbCln3+/+, left panel) and heterozygous (CbCln3+/Δex7/8, middle panel) cells, dextran-FITC label was observed in a perinuclear-clustered vesicular pattern with scattered labeled vesicles also present in the periphery. In contrast, dextran-FITC label of homozygous mutant (CbCln3Δex7/8/Δex7/8, right panel) cells was reduced overall and exhibited smaller stained vesicles with less perinuclear clustering. Confocal images were captured with identical exposure settings. 40 × magnification."}