PMC:539297 / 17823-18418
Annnotations
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T3152","span":{"begin":0,"end":1},"obj":"UBERON:0002037"},{"id":"T3153","span":{"begin":1,"end":2},"obj":"GO:0005764"},{"id":"T3154","span":{"begin":9,"end":10},"obj":"GO:0042571"},{"id":"T3155","span":{"begin":25,"end":26},"obj":"GO:0005634"},{"id":"T3156","span":{"begin":31,"end":32},"obj":"GO:0005634"},{"id":"T3157","span":{"begin":51,"end":55},"obj":"PR:000006901"},{"id":"T3158","span":{"begin":101,"end":124},"obj":"GO:0006907"},{"id":"T3159","span":{"begin":156,"end":158},"obj":"UBERON:0002037"},{"id":"T3160","span":{"begin":158,"end":162},"obj":"PR:000005591"},{"id":"T3161","span":{"begin":191,"end":198},"obj":"CHEBI:52071"},{"id":"T3162","span":{"begin":199,"end":203},"obj":"CHEBI:37926"},{"id":"T3163","span":{"begin":274,"end":281},"obj":"CHEBI:52071"},{"id":"T3164","span":{"begin":282,"end":286},"obj":"CHEBI:37926"},{"id":"T3165","span":{"begin":355,"end":364},"obj":"GO:0006897"},{"id":"T3166","span":{"begin":355,"end":373},"obj":"GO:0030139"},{"id":"T3167","span":{"begin":405,"end":412},"obj":"GO:0005634"},{"id":"T3168","span":{"begin":441,"end":443},"obj":"UBERON:0002037"},{"id":"T3169","span":{"begin":443,"end":447},"obj":"PR:000005591"},{"id":"T3170","span":{"begin":497,"end":504},"obj":"CHEBI:52071"},{"id":"T3171","span":{"begin":505,"end":509},"obj":"CHEBI:37926"},{"id":"T3172","span":{"begin":539,"end":547},"obj":"GO:0031982"},{"id":"T3173","span":{"begin":573,"end":582},"obj":"GO:0005737"}],"text":"Consistent with the altered early endosome marker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7). Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region. However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell."}
craft-sa-dev
{"project":"craft-sa-dev","denotations":[{"id":"T3629","span":{"begin":0,"end":12},"obj":"sentence"},{"id":"T3695","span":{"begin":176,"end":178},"obj":"IN"},{"id":"T3696","span":{"begin":179,"end":187},"obj":"VBN"},{"id":"T3697","span":{"begin":188,"end":190},"obj":"IN"},{"id":"T3698","span":{"begin":191,"end":198},"obj":"NN"},{"id":"T3699","span":{"begin":199,"end":203},"obj":"NN"},{"id":"T3700","span":{"begin":198,"end":199},"obj":"HYPH"},{"id":"T3701","span":{"begin":204,"end":210},"obj":"NN"},{"id":"T3702","span":{"begin":211,"end":212},"obj":"-LRB-"},{"id":"T3703","span":{"begin":212,"end":216},"obj":"NN"},{"id":"T3704","span":{"begin":217,"end":218},"obj":"CD"},{"id":"T3705","span":{"begin":218,"end":219},"obj":"-RRB-"},{"id":"T3706","span":{"begin":219,"end":220},"obj":"."},{"id":"T3707","span":{"begin":220,"end":420},"obj":"sentence"},{"id":"T3708","span":{"begin":221,"end":230},"obj":"VBG"},{"id":"T3709","span":{"begin":321,"end":330},"obj":"VBD"},{"id":"T3710","span":{"begin":231,"end":232},"obj":"DT"},{"id":"T3711","span":{"begin":243,"end":253},"obj":"NN"},{"id":"T3712","span":{"begin":233,"end":235},"obj":"CD"},{"id":"T3713","span":{"begin":236,"end":242},"obj":"NN"},{"id":"T3749","span":{"begin":451,"end":452},"obj":"HYPH"},{"id":"T3750","span":{"begin":452,"end":453},"obj":"CD"},{"id":"T3751","span":{"begin":460,"end":464},"obj":"VBD"},{"id":"T3752","span":{"begin":465,"end":469},"obj":"RBR"},{"id":"T3753","span":{"begin":470,"end":478},"obj":"RB"},{"id":"T3754","span":{"begin":487,"end":491},"obj":"IN"},{"id":"T3755","span":{"begin":492,"end":496},"obj":"JJS"},{"id":"T3756","span":{"begin":510,"end":516},"obj":"NN"},{"id":"T3757","span":{"begin":497,"end":504},"obj":"NN"},{"id":"T3758","span":{"begin":505,"end":509},"obj":"NN"},{"id":"T3759","span":{"begin":504,"end":505},"obj":"HYPH"},{"id":"T3760","span":{"begin":517,"end":527},"obj":"VBG"},{"id":"T3761","span":{"begin":528,"end":530},"obj":"IN"},{"id":"T3762","span":{"begin":531,"end":538},"obj":"JJR"},{"id":"T3763","span":{"begin":539,"end":547},"obj":"NNS"},{"id":"T3764","span":{"begin":548,"end":557},"obj":"VBN"},{"id":"T3765","span":{"begin":558,"end":568},"obj":"IN"},{"id":"T3766","span":{"begin":569,"end":572},"obj":"DT"},{"id":"T3767","span":{"begin":573,"end":582},"obj":"NN"},{"id":"T3768","span":{"begin":583,"end":585},"obj":"IN"},{"id":"T3769","span":{"begin":586,"end":589},"obj":"DT"},{"id":"T3770","span":{"begin":590,"end":594},"obj":"NN"},{"id":"T3771","span":{"begin":594,"end":595},"obj":"."},{"id":"T3675","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T3630","span":{"begin":11,"end":12},"obj":"VBN"},{"id":"T3631","span":{"begin":1,"end":2},"obj":"NNS"},{"id":"T3632","span":{"begin":0,"end":1},"obj":"NN"},{"id":"T3633","span":{"begin":2,"end":3},"obj":"NN"},{"id":"T3634","span":{"begin":3,"end":4},"obj":"JJ"},{"id":"T3635","span":{"begin":4,"end":5},"obj":"IN"},{"id":"T3636","span":{"begin":5,"end":8},"obj":"NN"},{"id":"T3637","span":{"begin":9,"end":10},"obj":"NN"},{"id":"T3638","span":{"begin":8,"end":9},"obj":"CC"},{"id":"T3639","span":{"begin":12,"end":17},"obj":"sentence"},{"id":"T3640","span":{"begin":12,"end":13},"obj":"JJ"},{"id":"T3641","span":{"begin":13,"end":14},"obj":"VBN"},{"id":"T3642","span":{"begin":14,"end":15},"obj":"DT"},{"id":"T3643","span":{"begin":16,"end":17},"obj":"NN"},{"id":"T3644","span":{"begin":17,"end":26},"obj":"sentence"},{"id":"T3645","span":{"begin":17,"end":18},"obj":"JJ"},{"id":"T3646","span":{"begin":18,"end":19},"obj":"NNS"},{"id":"T3647","span":{"begin":20,"end":22},"obj":"JJR"},{"id":"T3648","span":{"begin":22,"end":23},"obj":"JJ"},{"id":"T3649","span":{"begin":23,"end":26},"obj":"JJ"},{"id":"T3650","span":{"begin":26,"end":38},"obj":"sentence"},{"id":"T3651","span":{"begin":26,"end":27},"obj":"VBD"},{"id":"T3652","span":{"begin":28,"end":29},"obj":"VBN"},{"id":"T3653","span":{"begin":29,"end":30},"obj":"NN"},{"id":"T3654","span":{"begin":30,"end":32},"obj":"JJ"},{"id":"T3655","span":{"begin":32,"end":33},"obj":"JJ"},{"id":"T3656","span":{"begin":34,"end":35},"obj":"NN"},{"id":"T3657","span":{"begin":35,"end":36},"obj":"IN"},{"id":"T3658","span":{"begin":36,"end":37},"obj":"VBN"},{"id":"T3659","span":{"begin":37,"end":38},"obj":"NN"},{"id":"T3660","span":{"begin":38,"end":43},"obj":"sentence"},{"id":"T3661","span":{"begin":40,"end":42},"obj":"NNS"},{"id":"T3662","span":{"begin":38,"end":39},"obj":"JJ"},{"id":"T3663","span":{"begin":39,"end":40},"obj":"JJ"},{"id":"T3664","span":{"begin":43,"end":45},"obj":"sentence"},{"id":"T3665","span":{"begin":43,"end":45},"obj":"NN"},{"id":"T3666","span":{"begin":45,"end":220},"obj":"sentence"},{"id":"T3667","span":{"begin":134,"end":141},"obj":"VBN"},{"id":"T3668","span":{"begin":57,"end":63},"obj":"NN"},{"id":"T3669","span":{"begin":45,"end":46},"obj":"JJ"},{"id":"T3670","span":{"begin":46,"end":49},"obj":"NN"},{"id":"T3671","span":{"begin":50,"end":51},"obj":"-LRB-"},{"id":"T3672","span":{"begin":51,"end":55},"obj":"NN"},{"id":"T3673","span":{"begin":55,"end":56},"obj":"-RRB-"},{"id":"T3674","span":{"begin":64,"end":72},"obj":"VBN"},{"id":"T3676","span":{"begin":76,"end":90},"obj":"NN"},{"id":"T3677","span":{"begin":91,"end":92},"obj":"-LRB-"},{"id":"T3678","span":{"begin":92,"end":96},"obj":"NN"},{"id":"T3679","span":{"begin":97,"end":98},"obj":"CD"},{"id":"T3680","span":{"begin":98,"end":99},"obj":"-RRB-"},{"id":"T3681","span":{"begin":99,"end":101},"obj":","},{"id":"T3682","span":{"begin":101,"end":106},"obj":"NN"},{"id":"T3683","span":{"begin":107,"end":112},"obj":"NN"},{"id":"T3684","span":{"begin":106,"end":107},"obj":"HYPH"},{"id":"T3685","span":{"begin":113,"end":124},"obj":"NN"},{"id":"T3686","span":{"begin":125,"end":128},"obj":"VBD"},{"id":"T3687","span":{"begin":129,"end":133},"obj":"RB"},{"id":"T3688","span":{"begin":142,"end":144},"obj":"IN"},{"id":"T3689","span":{"begin":145,"end":155},"obj":"JJ"},{"id":"T3690","span":{"begin":169,"end":174},"obj":"NNS"},{"id":"T3691","span":{"begin":156,"end":166},"obj":"NN"},{"id":"T3692","span":{"begin":166,"end":167},"obj":"HYPH"},{"id":"T3693","span":{"begin":167,"end":168},"obj":"CD"},{"id":"T3694","span":{"begin":174,"end":176},"obj":","},{"id":"T3714","span":{"begin":235,"end":236},"obj":"HYPH"},{"id":"T3715","span":{"begin":254,"end":256},"obj":"IN"},{"id":"T3716","span":{"begin":257,"end":262},"obj":"NNS"},{"id":"T3717","span":{"begin":263,"end":273},"obj":"VBG"},{"id":"T3718","span":{"begin":274,"end":281},"obj":"NN"},{"id":"T3719","span":{"begin":282,"end":286},"obj":"NN"},{"id":"T3720","span":{"begin":281,"end":282},"obj":"HYPH"},{"id":"T3721","span":{"begin":286,"end":288},"obj":","},{"id":"T3722","span":{"begin":288,"end":292},"obj":"JJ"},{"id":"T3723","span":{"begin":293,"end":297},"obj":"NN"},{"id":"T3724","span":{"begin":292,"end":293},"obj":"HYPH"},{"id":"T3725","span":{"begin":315,"end":320},"obj":"NNS"},{"id":"T3726","span":{"begin":298,"end":301},"obj":"CC"},{"id":"T3727","span":{"begin":302,"end":314},"obj":"NN"},{"id":"T3728","span":{"begin":331,"end":339},"obj":"RB"},{"id":"T3729","span":{"begin":340,"end":347},"obj":"VBN"},{"id":"T3730","span":{"begin":365,"end":373},"obj":"NNS"},{"id":"T3731","span":{"begin":347,"end":349},"obj":","},{"id":"T3732","span":{"begin":349,"end":354},"obj":"JJ"},{"id":"T3733","span":{"begin":355,"end":364},"obj":"JJ"},{"id":"T3734","span":{"begin":374,"end":378},"obj":"WDT"},{"id":"T3735","span":{"begin":384,"end":393},"obj":"VBN"},{"id":"T3736","span":{"begin":379,"end":383},"obj":"VBD"},{"id":"T3737","span":{"begin":394,"end":396},"obj":"IN"},{"id":"T3738","span":{"begin":397,"end":400},"obj":"DT"},{"id":"T3739","span":{"begin":413,"end":419},"obj":"NN"},{"id":"T3740","span":{"begin":401,"end":412},"obj":"JJ"},{"id":"T3741","span":{"begin":419,"end":420},"obj":"."},{"id":"T3742","span":{"begin":420,"end":595},"obj":"sentence"},{"id":"T3743","span":{"begin":421,"end":428},"obj":"RB"},{"id":"T3744","span":{"begin":479,"end":486},"obj":"VBN"},{"id":"T3745","span":{"begin":428,"end":430},"obj":","},{"id":"T3746","span":{"begin":430,"end":440},"obj":"JJ"},{"id":"T3747","span":{"begin":454,"end":459},"obj":"NNS"},{"id":"T3748","span":{"begin":441,"end":451},"obj":"NN"}],"relations":[{"id":"R2079","pred":"compound","subj":"T3683","obj":"T3685"},{"id":"R2080","pred":"punct","subj":"T3684","obj":"T3683"},{"id":"R2081","pred":"nsubjpass","subj":"T3685","obj":"T3667"},{"id":"R2082","pred":"auxpass","subj":"T3686","obj":"T3667"},{"id":"R2083","pred":"advmod","subj":"T3687","obj":"T3667"},{"id":"R2084","pred":"prep","subj":"T3688","obj":"T3667"},{"id":"R2085","pred":"amod","subj":"T3689","obj":"T3690"},{"id":"R2086","pred":"pobj","subj":"T3690","obj":"T3688"},{"id":"R2087","pred":"nmod","subj":"T3691","obj":"T3690"},{"id":"R2088","pred":"punct","subj":"T3692","obj":"T3691"},{"id":"R2089","pred":"nummod","subj":"T3693","obj":"T3691"},{"id":"R2090","pred":"punct","subj":"T3694","obj":"T3667"},{"id":"R2091","pred":"mark","subj":"T3695","obj":"T3696"},{"id":"R2092","pred":"advcl","subj":"T3696","obj":"T3667"},{"id":"R2093","pred":"agent","subj":"T3697","obj":"T3696"},{"id":"R2094","pred":"compound","subj":"T3698","obj":"T3699"},{"id":"R2095","pred":"compound","subj":"T3699","obj":"T3701"},{"id":"R2096","pred":"punct","subj":"T3700","obj":"T3699"},{"id":"R2097","pred":"pobj","subj":"T3701","obj":"T3697"},{"id":"R2098","pred":"punct","subj":"T3702","obj":"T3703"},{"id":"R2099","pred":"parataxis","subj":"T3703","obj":"T3667"},{"id":"R2100","pred":"nummod","subj":"T3704","obj":"T3703"},{"id":"R2101","pred":"punct","subj":"T3705","obj":"T3703"},{"id":"R2102","pred":"punct","subj":"T3706","obj":"T3667"},{"id":"R2103","pred":"prep","subj":"T3708","obj":"T3709"},{"id":"R2104","pred":"det","subj":"T3710","obj":"T3711"},{"id":"R2105","pred":"pobj","subj":"T3711","obj":"T3708"},{"id":"R2106","pred":"nummod","subj":"T3712","obj":"T3713"},{"id":"R2107","pred":"compound","subj":"T3713","obj":"T3711"},{"id":"R2108","pred":"punct","subj":"T3714","obj":"T3713"},{"id":"R2109","pred":"prep","subj":"T3715","obj":"T3711"},{"id":"R2110","pred":"pobj","subj":"T3716","obj":"T3715"},{"id":"R2111","pred":"acl","subj":"T3717","obj":"T3716"},{"id":"R2112","pred":"compound","subj":"T3718","obj":"T3719"},{"id":"R2113","pred":"dobj","subj":"T3719","obj":"T3717"},{"id":"R2114","pred":"punct","subj":"T3720","obj":"T3719"},{"id":"R2115","pred":"punct","subj":"T3721","obj":"T3709"},{"id":"R2116","pred":"amod","subj":"T3722","obj":"T3723"},{"id":"R2117","pred":"nmod","subj":"T3723","obj":"T3725"},{"id":"R2118","pred":"punct","subj":"T3724","obj":"T3723"},{"id":"R2119","pred":"nsubj","subj":"T3725","obj":"T3709"},{"id":"R2120","pred":"cc","subj":"T3726","obj":"T3723"},{"id":"R2121","pred":"conj","subj":"T3727","obj":"T3723"},{"id":"R2122","pred":"advmod","subj":"T3728","obj":"T3729"},{"id":"R2123","pred":"amod","subj":"T3729","obj":"T3730"},{"id":"R2124","pred":"dobj","subj":"T3730","obj":"T3709"},{"id":"R2125","pred":"punct","subj":"T3731","obj":"T3730"},{"id":"R2126","pred":"amod","subj":"T3732","obj":"T3730"},{"id":"R2127","pred":"amod","subj":"T3733","obj":"T3730"},{"id":"R2128","pred":"dep","subj":"T3734","obj":"T3735"},{"id":"R2129","pred":"relcl","subj":"T3735","obj":"T3730"},{"id":"R2130","pred":"auxpass","subj":"T3736","obj":"T3735"},{"id":"R2131","pred":"prep","subj":"T3737","obj":"T3735"},{"id":"R2132","pred":"det","subj":"T3738","obj":"T3739"},{"id":"R2133","pred":"pobj","subj":"T3739","obj":"T3737"},{"id":"R2134","pred":"amod","subj":"T3740","obj":"T3739"},{"id":"R2135","pred":"punct","subj":"T3741","obj":"T3709"},{"id":"R2136","pred":"advmod","subj":"T3743","obj":"T3744"},{"id":"R2137","pred":"punct","subj":"T3745","obj":"T3744"},{"id":"R2138","pred":"amod","subj":"T3746","obj":"T3747"},{"id":"R2139","pred":"nsubjpass","subj":"T3747","obj":"T3744"},{"id":"R2140","pred":"nmod","subj":"T3748","obj":"T3747"},{"id":"R2141","pred":"punct","subj":"T3749","obj":"T3748"},{"id":"R2142","pred":"nummod","subj":"T3750","obj":"T3748"},{"id":"R2143","pred":"auxpass","subj":"T3751","obj":"T3744"},{"id":"R2144","pred":"advmod","subj":"T3752","obj":"T3753"},{"id":"R2145","pred":"advmod","subj":"T3753","obj":"T3744"},{"id":"R2146","pred":"prep","subj":"T3754","obj":"T3744"},{"id":"R2147","pred":"amod","subj":"T3755","obj":"T3756"},{"id":"R2148","pred":"nsubj","subj":"T3756","obj":"T3760"},{"id":"R2149","pred":"compound","subj":"T3757","obj":"T3758"},{"id":"R2150","pred":"compound","subj":"T3758","obj":"T3756"},{"id":"R2151","pred":"punct","subj":"T3759","obj":"T3758"},{"id":"R2152","pred":"pcomp","subj":"T3760","obj":"T3754"},{"id":"R2153","pred":"prep","subj":"T3761","obj":"T3760"},{"id":"R2154","pred":"amod","subj":"T3762","obj":"T3763"},{"id":"R2155","pred":"pobj","subj":"T3763","obj":"T3761"},{"id":"R2156","pred":"acl","subj":"T3764","obj":"T3763"},{"id":"R2157","pred":"prep","subj":"T3765","obj":"T3764"},{"id":"R2158","pred":"det","subj":"T3766","obj":"T3767"},{"id":"R2159","pred":"pobj","subj":"T3767","obj":"T3765"},{"id":"R2060","pred":"nmod","subj":"T3636","obj":"T3637"},{"id":"R2061","pred":"pobj","subj":"T3637","obj":"T3635"},{"id":"R2062","pred":"cc","subj":"T3638","obj":"T3636"},{"id":"R2057","pred":"nmod","subj":"T3632","obj":"T3631"},{"id":"R2058","pred":"nsubjpass","subj":"T3633","obj":"T3630"},{"id":"R2059","pred":"prep","subj":"T3635","obj":"T3633"},{"id":"R2063","pred":"amod","subj":"T3640","obj":"T3641"},{"id":"R2064","pred":"amod","subj":"T3645","obj":"T3646"},{"id":"R2065","pred":"amod","subj":"T3663","obj":"T3661"},{"id":"R2066","pred":"amod","subj":"T3669","obj":"T3668"},{"id":"R2067","pred":"nmod","subj":"T3670","obj":"T3668"},{"id":"R2068","pred":"punct","subj":"T3671","obj":"T3670"},{"id":"R2069","pred":"appos","subj":"T3672","obj":"T3670"},{"id":"R2070","pred":"punct","subj":"T3673","obj":"T3668"},{"id":"R2071","pred":"acl","subj":"T3674","obj":"T3668"},{"id":"R2072","pred":"prep","subj":"T3675","obj":"T3674"},{"id":"R2073","pred":"pobj","subj":"T3676","obj":"T3675"},{"id":"R2074","pred":"punct","subj":"T3677","obj":"T3678"},{"id":"R2075","pred":"nummod","subj":"T3679","obj":"T3678"},{"id":"R2076","pred":"punct","subj":"T3680","obj":"T3678"},{"id":"R2077","pred":"punct","subj":"T3681","obj":"T3667"},{"id":"R2078","pred":"compound","subj":"T3682","obj":"T3683"},{"id":"R2160","pred":"prep","subj":"T3768","obj":"T3767"},{"id":"R2161","pred":"det","subj":"T3769","obj":"T3770"},{"id":"R2162","pred":"pobj","subj":"T3770","obj":"T3768"},{"id":"R2163","pred":"punct","subj":"T3771","obj":"T3744"}],"text":"Consistent with the altered early endosome marker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7). Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region. However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T3264","span":{"begin":0,"end":1},"obj":"UBERON:0002037"},{"id":"T3265","span":{"begin":1,"end":2},"obj":"GO:0005764"},{"id":"T3266","span":{"begin":2,"end":3},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T3267","span":{"begin":9,"end":10},"obj":"GO:0042571"},{"id":"T3268","span":{"begin":16,"end":17},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T3269","span":{"begin":18,"end":19},"obj":"CL_GO_EXT:cell"},{"id":"T3270","span":{"begin":25,"end":26},"obj":"GO:0005634"},{"id":"T3271","span":{"begin":31,"end":32},"obj":"GO:0005634"},{"id":"T3272","span":{"begin":46,"end":49},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T3273","span":{"begin":51,"end":55},"obj":"PR_EXT:000006901"},{"id":"T3274","span":{"begin":101,"end":124},"obj":"GO:0006907"},{"id":"T3275","span":{"begin":156,"end":158},"obj":"UBERON:0002037"},{"id":"T3276","span":{"begin":158,"end":162},"obj":"PR_EXT:000005591"},{"id":"T3277","span":{"begin":169,"end":174},"obj":"CL_GO_EXT:cell"},{"id":"T3278","span":{"begin":191,"end":198},"obj":"CHEBI:52071"},{"id":"T3279","span":{"begin":199,"end":203},"obj":"CHEBI:37926"},{"id":"T3280","span":{"begin":274,"end":281},"obj":"CHEBI:52071"},{"id":"T3281","span":{"begin":282,"end":286},"obj":"CHEBI:37926"},{"id":"T3282","span":{"begin":288,"end":297},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T3283","span":{"begin":315,"end":320},"obj":"CL_GO_EXT:cell"},{"id":"T3284","span":{"begin":355,"end":364},"obj":"GO:0006897"},{"id":"T3285","span":{"begin":355,"end":373},"obj":"GO:0030139"},{"id":"T3286","span":{"begin":405,"end":412},"obj":"GO:0005634"},{"id":"T3287","span":{"begin":441,"end":443},"obj":"UBERON:0002037"},{"id":"T3288","span":{"begin":443,"end":447},"obj":"PR_EXT:000005591"},{"id":"T3289","span":{"begin":454,"end":459},"obj":"CL_GO_EXT:cell"},{"id":"T3290","span":{"begin":497,"end":504},"obj":"CHEBI:52071"},{"id":"T3291","span":{"begin":505,"end":509},"obj":"CHEBI:37926"},{"id":"T3292","span":{"begin":517,"end":527},"obj":"GO_PATO_EXT:biological_localization_process_or_quality"},{"id":"T3293","span":{"begin":539,"end":547},"obj":"GO:0031982"},{"id":"T3294","span":{"begin":573,"end":582},"obj":"GO:0005737"},{"id":"T3295","span":{"begin":590,"end":594},"obj":"CL_GO_EXT:cell"}],"text":"Consistent with the altered early endosome marker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7). Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region. However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell."}