PMC:539061 / 26401-27061 JSONTXT

{"project":"2_test","denotations":[{"id":"15630473-10660044-84810266","span":{"begin":275,"end":276},"obj":"10660044"},{"id":"T30922","span":{"begin":275,"end":276},"obj":"10660044"}],"text":"(E) Ajuba interacts with Grb-2 under conditions where α-catenin levels are reduced. Protein extracts were made from skins of P30 Wt and K14-Snail Tg P30 mice (blots on the left) and of newborn Wt and K14-Cre/α-catenin (fl/fl) conditionally null animals (blots on the right) [7]. Equal amounts of protein extracts were treated with anti-Grb-2 antibody (+) or control isotype antibody (–), and following centrifugation, immunoprecipitates were subjected to SDS-PAGE and Western blot analysis with anti-Ajuba and anti-Grb-2 antibodies. Note the presence of Ajuba only under conditions where levels of α-catenin and other AJ proteins were aberrantly low or absent."}