PMC:523229 / 26726-28235 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/523229","sourcedb":"PMC","sourceid":"523229","source_url":"http://www.ncbi.nlm.nih.gov/pmc/523229","text":"\nFigure 5 Bmpr1a Is Required to Maintain Expression of ECM Components in Articular Cartilage\nIn situ hybridization or LACZ staining on near adjacent sections of metacarpal-phalangeal joints (A–C and F–H) and the tarsal 2-metatarsal II joint (D–E and I–J) of P0 mice. At birth, articular cartilage of controls (A–E) and mutants (F–J) appears similar by Safranin O staining (A and F), and Col2 expression (B, G). Mat4 expression confirms that articular cartilage is initially specified in mutants (D andI, brackets). LACZ expression confirms Cre-mediated recombination has occurred in articular cartilage (C, H, E, and J). (K–T) Near adjacent sections of the metacarpal-phalangeal joints of P14 mice. Two weeks after birth, articular cartilage of controls stains with pericellular Safranin O (orange staining, K), and expresses Col2 (L), Agg (M), and SOX9 (N). In contrast, mutant articular cells are smaller and more densely packed, lack pericellular Safranin O staining (P), have reduced expression of Col2 (Q) and Agg (R), but retain normal levels of SOX9 protein (S, brackets; dashed line marks faint edges of articular surfaces). LACZ expression confirms Cre-mediated recombination has occurred in articular cells (O ansd T, brackets). (A and K) Scale bar = 75 μm. To determine whether articular cells were properly specified in mutants, we also analyzed expression of Matrilin-4 (Mat4), a gene expressed specifically in the periarticular and perichondral regions of developing joints (Klatt et al. 2001).","divisions":[{"label":"figure","span":{"begin":1,"end":1268}},{"label":"label","span":{"begin":1,"end":9}},{"label":"caption","span":{"begin":11,"end":1268}},{"label":"title","span":{"begin":11,"end":94}},{"label":"p","span":{"begin":94,"end":1268}}],"tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T6126","span":{"begin":0,"end":1509},"obj":"sentence"},{"id":"T6127","span":{"begin":1269,"end":1271},"obj":"TO"},{"id":"T6128","span":{"begin":1272,"end":1281},"obj":"VB"},{"id":"T6129","span":{"begin":1350,"end":1358},"obj":"VBD"},{"id":"T6130","span":{"begin":1282,"end":1289},"obj":"IN"},{"id":"T6131","span":{"begin":1320,"end":1329},"obj":"VBN"},{"id":"T6132","span":{"begin":1290,"end":1299},"obj":"JJ"},{"id":"T6133","span":{"begin":1300,"end":1305},"obj":"NNS"},{"id":"T6134","span":{"begin":1306,"end":1310},"obj":"VBD"},{"id":"T6135","span":{"begin":1311,"end":1319},"obj":"RB"},{"id":"T6136","span":{"begin":1330,"end":1332},"obj":"IN"},{"id":"T6137","span":{"begin":1333,"end":1340},"obj":"NNS"},{"id":"T6138","span":{"begin":1340,"end":1342},"obj":","},{"id":"T6139","span":{"begin":1342,"end":1344},"obj":"PRP"},{"id":"T6140","span":{"begin":1345,"end":1349},"obj":"RB"},{"id":"T6141","span":{"begin":1359,"end":1369},"obj":"NN"},{"id":"T6142","span":{"begin":1370,"end":1372},"obj":"IN"},{"id":"T6143","span":{"begin":1373,"end":1381},"obj":"NN"},{"id":"T6144","span":{"begin":1381,"end":1382},"obj":"HYPH"},{"id":"T6145","span":{"begin":1382,"end":1383},"obj":"CD"},{"id":"T6146","span":{"begin":1384,"end":1385},"obj":"-LRB-"},{"id":"T6147","span":{"begin":1385,"end":1389},"obj":"NN"},{"id":"T6148","span":{"begin":1389,"end":1390},"obj":"-RRB-"},{"id":"T6149","span":{"begin":1390,"end":1392},"obj":","},{"id":"T6150","span":{"begin":1392,"end":1393},"obj":"DT"},{"id":"T6151","span":{"begin":1394,"end":1398},"obj":"NN"},{"id":"T6152","span":{"begin":1399,"end":1408},"obj":"VBN"},{"id":"T6153","span":{"begin":1409,"end":1421},"obj":"RB"},{"id":"T6154","span":{"begin":1422,"end":1424},"obj":"IN"},{"id":"T6155","span":{"begin":1425,"end":1428},"obj":"DT"},{"id":"T6156","span":{"begin":1460,"end":1467},"obj":"NNS"},{"id":"T6157","span":{"begin":1429,"end":1442},"obj":"JJ"},{"id":"T6158","span":{"begin":1443,"end":1446},"obj":"CC"},{"id":"T6159","span":{"begin":1447,"end":1459},"obj":"JJ"},{"id":"T6160","span":{"begin":1468,"end":1470},"obj":"IN"},{"id":"T6161","span":{"begin":1471,"end":1481},"obj":"VBG"},{"id":"T6162","span":{"begin":1482,"end":1488},"obj":"NNS"},{"id":"T6163","span":{"begin":1489,"end":1490},"obj":"-LRB-"},{"id":"T6164","span":{"begin":1490,"end":1495},"obj":"NNP"},{"id":"T6165","span":{"begin":1496,"end":1498},"obj":"FW"},{"id":"T6166","span":{"begin":1499,"end":1502},"obj":"FW"},{"id":"T6167","span":{"begin":1503,"end":1507},"obj":"CD"},{"id":"T6168","span":{"begin":1507,"end":1508},"obj":"-RRB-"},{"id":"T6169","span":{"begin":1508,"end":1509},"obj":"."}],"relations":[{"id":"R3778","pred":"aux","subj":"T6127","obj":"T6128"},{"id":"R3779","pred":"advcl","subj":"T6128","obj":"T6129"},{"id":"R3780","pred":"mark","subj":"T6130","obj":"T6131"},{"id":"R3781","pred":"ccomp","subj":"T6131","obj":"T6128"},{"id":"R3782","pred":"amod","subj":"T6132","obj":"T6133"},{"id":"R3783","pred":"nsubjpass","subj":"T6133","obj":"T6131"},{"id":"R3784","pred":"auxpass","subj":"T6134","obj":"T6131"},{"id":"R3785","pred":"advmod","subj":"T6135","obj":"T6131"},{"id":"R3786","pred":"prep","subj":"T6136","obj":"T6131"},{"id":"R3787","pred":"pobj","subj":"T6137","obj":"T6136"},{"id":"R3788","pred":"punct","subj":"T6138","obj":"T6129"},{"id":"R3789","pred":"nsubj","subj":"T6139","obj":"T6129"},{"id":"R3790","pred":"advmod","subj":"T6140","obj":"T6129"},{"id":"R3791","pred":"dobj","subj":"T6141","obj":"T6129"},{"id":"R3792","pred":"prep","subj":"T6142","obj":"T6141"},{"id":"R3793","pred":"pobj","subj":"T6143","obj":"T6142"},{"id":"R3794","pred":"punct","subj":"T6144","obj":"T6143"},{"id":"R3795","pred":"nummod","subj":"T6145","obj":"T6143"},{"id":"R3796","pred":"punct","subj":"T6146","obj":"T6143"},{"id":"R3797","pred":"appos","subj":"T6147","obj":"T6143"},{"id":"R3798","pred":"punct","subj":"T6148","obj":"T6143"},{"id":"R3799","pred":"punct","subj":"T6149","obj":"T6143"},{"id":"R3800","pred":"det","subj":"T6150","obj":"T6151"},{"id":"R3801","pred":"appos","subj":"T6151","obj":"T6143"},{"id":"R3802","pred":"acl","subj":"T6152","obj":"T6151"},{"id":"R3803","pred":"advmod","subj":"T6153","obj":"T6152"},{"id":"R3804","pred":"prep","subj":"T6154","obj":"T6152"},{"id":"R3805","pred":"det","subj":"T6155","obj":"T6156"},{"id":"R3806","pred":"pobj","subj":"T6156","obj":"T6154"},{"id":"R3807","pred":"amod","subj":"T6157","obj":"T6156"},{"id":"R3808","pred":"cc","subj":"T6158","obj":"T6157"},{"id":"R3809","pred":"conj","subj":"T6159","obj":"T6157"},{"id":"R3810","pred":"prep","subj":"T6160","obj":"T6156"},{"id":"R3811","pred":"amod","subj":"T6161","obj":"T6162"},{"id":"R3812","pred":"pobj","subj":"T6162","obj":"T6160"},{"id"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