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Endolymph volume in Slc26a4-/- mice was increased and tissue masses in areas normally occupied by type I and II fibrocytes were reduced. Slc26a4-/- mice lacked the endocochlear potential, which is generated across the basal cell barrier by the K+ channel KCNJ10 localized in intermediate cells. Stria vascularis was hyperpigmented, suggesting unalleviated free radical damage. The basal cell barrier appeared intact; intermediate cells and KCNJ10 mRNA were present but KCNJ10 protein was absent. Endolymphatic K+ concentrations were normal and membrane proteins necessary for K+ secretion were present, including the K+ channel KCNQ1 and KCNE1, Na+/2Cl-/K+ cotransporter SLC12A2 and the gap junction GJB2."}

    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T97","span":{"begin":0,"end":7},"obj":"PR:000015039"},{"id":"T98","span":{"begin":25,"end":32},"obj":"UBERON:0001844"},{"id":"T99","span":{"begin":36,"end":55},"obj":"_FRAGMENT"},{"id":"T100","span":{"begin":74,"end":79},"obj":"GO:0016324"},{"id":"T101","span":{"begin":56,"end":73},"obj":"UBERON:0028194"},{"id":"T102","span":{"begin":108,"end":124},"obj":"UBERON:0002282"},{"id":"T103","span":{"begin":129,"end":141},"obj":"UBERON:0008832"},{"id":"T104","span":{"begin":158,"end":167},"obj":"UBERON:0001852"},{"id":"T105","span":{"begin":178,"end":185},"obj":"PR:000015039"},{"id":"T106","span":{"begin":189,"end":193},"obj":"NCBITaxon:10088"},{"id":"T107","span":{"begin":212,"end":218},"obj":"UBERON:0000479"},{"id":"T108","span":{"begin":256,"end":262},"obj":"_FRAGMENT"},{"id":"T109","span":{"begin":270,"end":280},"obj":"CL:0002670"},{"id":"T110","span":{"begin":256,"end":260},"obj":"_FRAGMENT"},{"id":"T111","span":{"begin":267,"end":280},"obj":"CL:0002666"},{"id":"T112","span":{"begin":295,"end":302},"obj":"PR:000015039"},{"id":"T113","span":{"begin":306,"end":310},"obj":"NCBITaxon:10088"},{"id":"T114","span":{"begin":376,"end":386},"obj":"CL:0000646"},{"id":"T115","span":{"begin":402,"end":404},"obj":"CHEBI:29103"},{"id":"T116","span":{"begin":413,"end":419},"obj":"PR:000001979"},{"id":"T117","span":{"begin":433,"end":451},"obj":"CL:0002486"},{"id":"T118","span":{"begin":453,"end":469},"obj":"UBERON:0002282"},{"id":"T119","span":{"begin":479,"end":488},"obj":"CHEBI:26130"},{"id":"T120","span":{"begin":514,"end":526},"obj":"CHEBI:26519"},{"id":"T121","span":{"begin":539,"end":549},"obj":"CL:0000646"},{"id":"T122","span":{"begin":575,"end":593},"obj":"CL:0002486"},{"id":"T123","span":{"begin":598,"end":604},"obj":"PR:000001979"},{"id":"T124","span":{"begin":627,"end":633},"obj":"PR:000001979"},{"id":"T125","span":{"begin":654,"end":667},"obj":"UBERON:0001852"},{"id":"T126","span":{"begin":668,"end":670},"obj":"CHEBI:29103"},{"id":"T127","span":{"begin":702,"end":710},"obj":"GO:0016020"},{"id":"T128","span":{"begin":734,"end":736},"obj":"CHEBI:29103"},{"id":"T129","span":{"begin":775,"end":777},"obj":"CHEBI:29103"},{"id":"T130","span":{"begin":786,"end":791},"obj":"PR:000000728"},{"id":"T131","span":{"begin":796,"end":801},"obj":"PR:000009255"},{"id":"T132","span":{"begin":803,"end":806},"obj":"CHEBI:29101"},{"id":"T133","span":{"begin":812,"end":814},"obj":"CHEBI:29103"},{"id":"T134","span":{"begin":829,"end":836},"obj":"PR:000014925"},{"id":"T135","span":{"begin":845,"end":857},"obj":"GO:0005921"},{"id":"T136","span":{"begin":858,"end":862},"obj":"PR:000007998"}],"relations":[{"id":"R1","pred":"_lexicallyChainedTo","subj":"T100","obj":"T99"},{"id":"R2","pred":"_lexicallyChainedTo","subj":"T109","obj":"T108"},{"id":"R3","pred":"_lexicallyChainedTo","subj":"T111","obj":"T110"}],"text":"Pendrin was found in the cochlea in apical membranes of spiral prominence cells and spindle-shaped cells of stria vascularis, in outer sulcus and root cells. Endolymph volume in Slc26a4-/- mice was increased and tissue masses in areas normally occupied by type I and II fibrocytes were reduced. Slc26a4-/- mice lacked the endocochlear potential, which is generated across the basal cell barrier by the K+ channel KCNJ10 localized in intermediate cells. Stria vascularis was hyperpigmented, suggesting unalleviated free radical damage. The basal cell barrier appeared intact; intermediate cells and KCNJ10 mRNA were present but KCNJ10 protein was absent. Endolymphatic K+ concentrations were normal and membrane proteins necessary for K+ secretion were present, including the K+ channel KCNQ1 and KCNE1, Na+/2Cl-/K+ cotransporter SLC12A2 and the gap junction GJB2."}