PMC:5069600 / 14903-15518 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/5069600","sourcedb":"PMC","sourceid":"5069600","source_url":"https://www.ncbi.nlm.nih.gov/pmc/5069600","text":"Western blotting\nWestern blotting was performed using sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Briefly, 20‐μg homogenates of sural nerve were resuspended in a reduced sample buffer, electrophoresed on a 10% Tris gel with Tris running buffer, blotted to a polyvinylidene difluoride membrane, and sequentially probed with polyclonal rabbit antihuman CD10 antibody (Abcam). The membrane was subsequently incubated with a horseradish peroxidase–labeled polymer‐conjugated antimouse antibody reagent (EnVision+ reagent; Dako, Tokyo, Japan). 3‐3′‐diaminobenzidine was used for chromogenic visualization.","divisions":[{"label":"Title","span":{"begin":0,"end":16}}],"tracks":[]}