Western blotting
Western blotting was performed using sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Briefly, 20‐μg homogenates of sural nerve were resuspended in a reduced sample buffer, electrophoresed on a 10% Tris gel with Tris running buffer, blotted to a polyvinylidene difluoride membrane, and sequentially probed with polyclonal rabbit antihuman CD10 antibody (Abcam). The membrane was subsequently incubated with a horseradish peroxidase–labeled polymer‐conjugated antimouse antibody reagent (EnVision+ reagent; Dako, Tokyo, Japan). 3‐3′‐diaminobenzidine was used for chromogenic visualization.