PMC:4996395 / 26803-28940 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4996395","sourcedb":"PMC","sourceid":"4996395","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4996395","text":"6. Conclusions and Future Perspectives\nThe development of new strategies for protein profiling may improve analysis and reduce the time for screening of thousands of proteins simultaneously. In addition, the expression of the proteins at the moment of usage improves the quality of the proteins, since the final product does not suffer any change related to temperature, pH or degradation. The use of mammalian ribosomes and chaperone proteins contributes to accurate protein folding. Unlike conventional protein arrays, nucleic acid programmable protein arrays (NAPPA) are DNA-based arrays that convert into protein microarrays. A key advantage of this approach is its adaptability. To display a different set of proteins—the proteins of a recently discovered pathogen, a set of proteins related to a gene family, or a series of mutant versions of a protein of interest—the practitioner merely has to produce the DNA clones encoding the proteins. There is no need to go through a long development process of purifying the proteins. To produce the protein of interest, the coding-DNA inserted into a plasmid is printed onto a surface and then is transcribed/translated by using a cell-free expression system. Moreover, once printed, the coding material remains intact, even at ambient temperature, thanks to the high stability of nucleic acids. The labile protein is not expressed until it is required.\nMany investigators have employed this NAPPA approach for their studies, including cancer, autoimmune diseases, host-pathogens interactions, quantification of protein binding kinetics, and infection responses to microorganisms showing a high specificity and selectivity with accurate and reproducible results. Moreover, it has been demonstrated that the platform is unbiased and independent of molecular size or protein family type.\nIn summary, NAPPA technology seems to be a powerful tool for performing HT analysis in different formats (planar, beads…), with different samples (sera, urine, saliva…) and combined with other platforms. All in all, clinical and diagnostic screenings may be accomplished in a rapid and reliable manner.","divisions":[{"label":"Title","span":{"begin":0,"end":38}}],"tracks":[]}