PMC:4719559 / 9467-10708
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4719559","sourcedb":"PMC","sourceid":"4719559","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4719559","text":"Peroneal nerve MSNA was recorded as described previously [22]. Briefly, the nerve was located with cutaneous electrical stimulation (Isostim A320, World Precision Instruments). A tungsten reference electrode (FHC, Bowdoinham, ME, USA) was inserted subcutaneously, ~2 cm from the nerve, and a tungsten recording electrode with an uninsulated tip diameter of ~10 μm was inserted through the skin near the nerve. Adjustments of the recording electrode position were made according to auditory signals generated by impaled nerves. Both electrodes were connected in series to a differential preamplifier and an amplifier (NASA, Houston, TX, USA), isolated by two 100 mA current limiters. The nerve signal was amplified (total gain 40,000–80,000), band-pass filtered (high pass of 0.7 kHz and low pass of 2–3 kHz), and then full-wave rectified and smoothed with a resistance–capacitance circuit (time constant, 0.1 s) to produce a recording of “integrated” MSNA. Satisfactory recordings of MSNA were defined by pulse-synchronous bursts that increased during end-expiratory apnoea or Valsalva straining and did not change during tactile or auditory stimulation. Due to technical issues, MSNA was only measured and analyzed during ambulatory phases.","tracks":[{"project":"TEST0","denotations":[{"id":"26793301-58-64-73092","span":{"begin":58,"end":60},"obj":"[\"6278945\"]"}],"attributes":[{"subj":"26793301-58-64-73092","pred":"source","obj":"TEST0"}]},{"project":"MyTest","denotations":[{"id":"26793301-6278945-30256711","span":{"begin":58,"end":60},"obj":"6278945"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"26793301-6278945-30256711","pred":"source","obj":"MyTest"}]},{"project":"0_colil","denotations":[{"id":"26793301-6278945-73092","span":{"begin":58,"end":60},"obj":"6278945"}],"attributes":[{"subj":"26793301-6278945-73092","pred":"source","obj":"0_colil"}]},{"project":"2_test","denotations":[{"id":"26793301-6278945-30256711","span":{"begin":58,"end":60},"obj":"6278945"}],"attributes":[{"subj":"26793301-6278945-30256711","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"TEST0","color":"#93c1ec","default":true},{"id":"MyTest","color":"#dbec93"},{"id":"0_colil","color":"#e393ec"},{"id":"2_test","color":"#93ecc9"}]}]}}