PMC:4502371 / 12375-13139 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4502371","sourcedb":"PMC","sourceid":"4502371","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4502371","text":"The bacteria-mediated feeding RNAi was performed as described (Fraser et al. 2000), with the following modifications. The mua-3(uy19) mutants were fed with the clones of dbl-1 and dpy-17 from the Ahringer feeding library (Fraser et al. 2000; Kamath and Ahringer 2003). The plates containing NGM agar with 1 mM IPTG and 50 mg/ml carbenicillin were inoculated with bacterial cultures grown 16–18 hr for each targeted gene. L4-stage C. elegans were transferred to the plates for each gene at 15°. Forty-eight hours later, adults were removed and the plates were placed at 25°. Forty-eight hours later, the number of progeny that survived was counted and the percentage was calculated. The RNAi clones of dpy-17 and dbl-1 were sequenced and validated (data not shown).","tracks":[{"project":"2_test","denotations":[{"id":"25917920-11099033-43299629","span":{"begin":77,"end":81},"obj":"11099033"},{"id":"25917920-11099033-43299630","span":{"begin":236,"end":240},"obj":"11099033"},{"id":"25917920-12828945-43299631","span":{"begin":262,"end":266},"obj":"12828945"}],"attributes":[{"subj":"25917920-11099033-43299629","pred":"source","obj":"2_test"},{"subj":"25917920-11099033-43299630","pred":"source","obj":"2_test"},{"subj":"25917920-12828945-43299631","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ece493","default":true}]}]}}