PMC:4502371 / 12375-13139 JSONTXT

{"project":"2_test","denotations":[{"id":"25917920-11099033-43299629","span":{"begin":77,"end":81},"obj":"11099033"},{"id":"25917920-11099033-43299630","span":{"begin":236,"end":240},"obj":"11099033"},{"id":"25917920-12828945-43299631","span":{"begin":262,"end":266},"obj":"12828945"}],"text":"The bacteria-mediated feeding RNAi was performed as described (Fraser et al. 2000), with the following modifications. The mua-3(uy19) mutants were fed with the clones of dbl-1 and dpy-17 from the Ahringer feeding library (Fraser et al. 2000; Kamath and Ahringer 2003). The plates containing NGM agar with 1 mM IPTG and 50 mg/ml carbenicillin were inoculated with bacterial cultures grown 16–18 hr for each targeted gene. L4-stage C. elegans were transferred to the plates for each gene at 15°. Forty-eight hours later, adults were removed and the plates were placed at 25°. Forty-eight hours later, the number of progeny that survived was counted and the percentage was calculated. The RNAi clones of dpy-17 and dbl-1 were sequenced and validated (data not shown)."}