PMC:4419340 / 33196-33767 JSONTXT

{"project":"2_test","denotations":[{"id":"25918342-18768888-59633064","span":{"begin":206,"end":210},"obj":"18768888"}],"text":"PBMC culture.\nFrozen PBMCs were cultured in the presence of 100 ng/ml thymic stromal lymphopoietin (R\u0026D Systems) in X-VIVO 15 (Lonza) plus 5% human AB serum (Lonza), as previously described (Rickel et al., 2008). PBMCs were collected, washed, and resuspended at a density of 4 × 106 cells/well in 48-well plates, in a final volume of 0.5 ml/well, in the presence of 10 ng/ml recombinant human IL-2 (R\u0026D Systems) and 10 ng/ml recombinant human IL-17E/IL-25 (R\u0026D Systems). After 3 d, the amount of IL-5 secreted into the medium was determined with ELISA kits (R\u0026D Systems)."}