PMC:4377207 / 36158-36573 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4377207","sourcedb":"PMC","sourceid":"4377207","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4377207","text":"Overexpression growth experiments on solid media\nYeast strains were grown overnight to saturation in appropriate media (YPD or SC-Ura media to maintain plasmids). These saturated cultures were serially diluted in 10-fold increments and frogged onto YPD or SC-URA plates containing 0 nM, 10 nM, 100 nM, 1 μM, or 10 μM β-estradiol (Tocris Biosciences). These plates were incubated at 30°C for two days before imaging.","divisions":[{"label":"title","span":{"begin":0,"end":48}}],"tracks":[]}