PMC:4236617 / 16431-17686 JSONTXT

{"project":"2_test","denotations":[{"id":"25070625-19695336-12189975","span":{"begin":1098,"end":1100},"obj":"19695336"}],"text":"Growth inhibition of intestinal pathogens by the culture supernatants of L. amylovorus\nThe supernatants collected (650 g, 20 min, +4°C) from overnight cultures of the L. amylovorus strains were filter-sterilized through 0.22 μm pore-size filters and stored at -20°C. The inhibitory effects of the supernatants were assessed by monitoring the abilities of the pathogens to grow in the presence (10% V/V) of the supernatants in a microtiter plate format as previously described [36]. Briefly, the A600nm values of the pathogen cultures were measured every 30 minutes with an automatic reader (Bioscreen C, Growth Curves Oy, Helsinki, Finland) in the presence or absence of pH-adjusted (pH 6.2) or non-adjusted culture supernatants at 36.5 +/-0.5°C, with three parallel wells for each supernatant and control. The inhibition was quantified using the area under the growth curve (AUC) obtained during the first 12 hours of growth, automatically created by the Research Express software (Transgalactic Ltd, Vantaa, Finland), and expressed as the area reduction percentage (ARP) as previously described [29]. Linear regression (SPSS) was used to estimate the relationship between the ARP values and colony forming unit (CFU) counts as previously described [36]."}

{"project":"MicrobeTaxon","denotations":[{"id":"T107","span":{"begin":73,"end":86},"obj":"1604"},{"id":"T108","span":{"begin":167,"end":180},"obj":"1604"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Growth inhibition of intestinal pathogens by the culture supernatants of L. amylovorus\nThe supernatants collected (650 g, 20 min, +4°C) from overnight cultures of the L. amylovorus strains were filter-sterilized through 0.22 μm pore-size filters and stored at -20°C. The inhibitory effects of the supernatants were assessed by monitoring the abilities of the pathogens to grow in the presence (10% V/V) of the supernatants in a microtiter plate format as previously described [36]. Briefly, the A600nm values of the pathogen cultures were measured every 30 minutes with an automatic reader (Bioscreen C, Growth Curves Oy, Helsinki, Finland) in the presence or absence of pH-adjusted (pH 6.2) or non-adjusted culture supernatants at 36.5 +/-0.5°C, with three parallel wells for each supernatant and control. The inhibition was quantified using the area under the growth curve (AUC) obtained during the first 12 hours of growth, automatically created by the Research Express software (Transgalactic Ltd, Vantaa, Finland), and expressed as the area reduction percentage (ARP) as previously described [29]. Linear regression (SPSS) was used to estimate the relationship between the ARP values and colony forming unit (CFU) counts as previously described [36]."}