PMC:4136538 / 36185-37193 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4136538","sourcedb":"PMC","sourceid":"4136538","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4136538","text":"Table 1 Primary monoclonal antibodies (mAb) used for flow cytometry.\nPrimary mAb Source Clone Isotype Stockconcentration Workingdilution\nSurface staining a APC-anti-mouse CD45 eBioscience 30-F11 Rat IgG2b 0.2 mg/ml 1:50\nFITC-anti-mouse CD4 BD Biosciences GK1.5 Rat IgG2b 0.5 mg/ml 1:50\nPerCP-anti-mouse CD3 BD Biosciences 145-2C11 Armenian Hamster IgG1 0.2 mg/ml 1:50\nIntracellular staining b PE-anti-mouse T-bet eBioscience eBio4B10 Mouse IgG1 0.2 mg/ml 1:100\nPE-anti-mouse GATA-3 eBioscience TWAJ Rat IgG2b 3 μg/ml 1:100\nPE-anti-mouse IFN-γ eBioscience XMG1.2 Rat IgG1 0.2 mg/ml 1:400\nPE-anti-mouse IL-4 eBioscience 11B11 Rat IgG1 0.2 mg/ml 1:100\nPE-anti-mouse TNF-α eBioscience MP6-XT22 Rat IgG1 0.2 mg/ml 1:100\nPE-anti-mouse GM-CSF eBioscience MP1-22E9 Rat IgG2a 0.2 mg/ml 1:200\na For each sample tube, all three mAbs for surface staining were added at the same time.\nb For each sample tube, one of these mAbs was used during intracellular staining.","divisions":[{"label":"label","span":{"begin":0,"end":7}},{"label":"caption","span":{"begin":9,"end":69}},{"label":"p","span":{"begin":9,"end":69}},{"label":"table","span":{"begin":70,"end":835}},{"label":"tr","span":{"begin":70,"end":142}},{"label":"th","span":{"begin":70,"end":81}},{"label":"th","span":{"begin":83,"end":89}},{"label":"th","span":{"begin":91,"end":96}},{"label":"th","span":{"begin":98,"end":105}},{"label":"th","span":{"begin":107,"end":125}},{"label":"th","span":{"begin":127,"end":142}},{"label":"tr","span":{"begin":143,"end":231}},{"label":"td","span":{"begin":143,"end":162}},{"label":"td","span":{"begin":163,"end":182}},{"label":"td","span":{"begin":184,"end":195}},{"label":"td","span":{"begin":197,"end":203}},{"label":"td","span":{"begin":205,"end":214}},{"label":"td","span":{"begin":216,"end":225}},{"label":"td","span":{"begin":227,"end":231}},{"label":"tr","span":{"begin":232,"end":302}},{"label":"td","span":{"begin":232,"end":251}},{"label":"td","span":{"begin":253,"end":267}},{"label":"td","span":{"begin":269,"end":274}},{"label":"td","span":{"begin":276,"end":285}},{"label":"td","span":{"begin":287,"end":296}},{"label":"td","span":{"begin":298,"end":302}},{"label":"tr","span":{"begin":303,"end":389}},{"label":"td","span":{"begin":303,"end":323}},{"label":"td","span":{"begin":325,"end":339}},{"label":"td","span":{"begin":341,"end":349}},{"label":"td","span":{"begin":351,"end":372}},{"label":"td","span":{"begin":374,"end":383}},{"label":"td","span":{"begin":385,"end":389}},{"label":"tr","span":{"begin":390,"end":488}},{"label":"td","span":{"begin":390,"end":415}},{"label":"td","span":{"begin":416,"end":435}},{"label":"td","span":{"begin":437,"end":448}},{"label":"td","span":{"begin":450,"end":458}},{"label":"td","span":{"begin":460,"end":470}},{"label":"td","span":{"begin":472,"end":481}},{"label":"td","span":{"begin":483,"end":488}},{"label":"tr","span":{"begin":489,"end":555}},{"label":"td","span":{"begin":489,"end":509}},{"label":"td","span":{"begin":511,"end":522}},{"label":"td","span":{"begin":524,"end":528}},{"label":"td","span":{"begin":530,"end":539}},{"label":"td","span":{"begin":541,"end":548}},{"label":"td","span":{"begin":550,"end":555}},{"label":"tr","span":{"begin":556,"end":624}},{"label":"td","span":{"begin":556,"end":575}},{"label":"td","span":{"begin":577,"end":588}},{"label":"td","span":{"begin":590,"end":596}},{"label":"td","span":{"begin":598,"end":606}},{"label":"td","span":{"begin":608,"end":617}},{"label":"td","span":{"begin":619,"end":624}},{"label":"tr","span":{"begin":625,"end":691}},{"label":"td","span":{"begin":625,"end":643}},{"label":"td","span":{"begin":645,"end":656}},{"label":"td","span":{"begin":658,"end":663}},{"label":"td","span":{"begin":665,"end":673}},{"label":"td","span":{"begin":675,"end":684}},{"label":"td","span":{"begin":686,"end":691}},{"label":"tr","span":{"begin":692,"end":762}},{"label":"td","span":{"begin":692,"end":711}},{"label":"td","span":{"begin":713,"end":724}},{"label":"td","span":{"begin":726,"end":734}},{"label":"td","span":{"begin":736,"end":744}},{"label":"td","span":{"begin":746,"end":755}},{"label":"td","span":{"begin":757,"end":762}},{"label":"tr","span":{"begin":763,"end":835}},{"label":"td","span":{"begin":763,"end":783}},{"label":"td","span":{"begin":785,"end":796}},{"label":"td","span":{"begin":798,"end":806}},{"label":"td","span":{"begin":808,"end":817}},{"label":"td","span":{"begin":819,"end":828}},{"label":"td","span":{"begin":830,"end":835}},{"label":"footnote","span":{"begin":836,"end":925}},{"label":"label","span":{"begin":836,"end":837}},{"label":"p","span":{"begin":839,"end":925}},{"label":"label","span":{"begin":926,"end":927}}],"tracks":[]}