Table 1  Primary monoclonal antibodies (mAb) used for flow cytometry.
Primary mAb  Source  Clone  Isotype  Stockconcentration  Workingdilution
Surface staining a  APC-anti-mouse CD45  eBioscience  30-F11  Rat IgG2b  0.2 mg/ml  1:50
FITC-anti-mouse CD4  BD Biosciences  GK1.5  Rat IgG2b  0.5 mg/ml  1:50
PerCP-anti-mouse CD3  BD Biosciences  145-2C11  Armenian Hamster IgG1  0.2 mg/ml  1:50
Intracellular staining b  PE-anti-mouse T-bet  eBioscience  eBio4B10  Mouse IgG1  0.2 mg/ml  1:100
PE-anti-mouse GATA-3  eBioscience  TWAJ  Rat IgG2b  3 μg/ml  1:100
PE-anti-mouse IFN-γ  eBioscience  XMG1.2  Rat IgG1  0.2 mg/ml  1:400
PE-anti-mouse IL-4  eBioscience  11B11  Rat IgG1  0.2 mg/ml  1:100
PE-anti-mouse TNF-α  eBioscience  MP6-XT22  Rat IgG1  0.2 mg/ml  1:100
PE-anti-mouse GM-CSF  eBioscience  MP1-22E9  Rat IgG2a  0.2 mg/ml  1:200
a  For each sample tube, all three mAbs for surface staining were added at the same time.
b  For each sample tube, one of these mAbs was used during intracellular staining.