PMC:4103454 / 23622-24241 JSONTXT

{"project":"2_test","denotations":[{"id":"24981866-20124702-20021755","span":{"begin":463,"end":467},"obj":"20124702"}],"text":"Crystallography\nCrystals were obtained by the sitting drop vapor diffusion method using the Morpheus crystallization screen (Molecular Dimensions; Supplemental Information). Diffraction data were collected at 100 K on beamline IO4 at the Diamond Light Source and processed with Denzo/Scalepack (HKL Research). The structures of selenomethionine containing derivatives of stNUC and psNUC were determined by automated SAD/model building using PHENIX (Adams et al., 2010). saNUC was solved by molecular replacement with PHENIX using stNUC as a search model. Models were improved and refined using Coot, Refmac, and PHENIX."}